L-Mimosine

Catalog No.S7446 Batch:S744604

Technical Data

Formula	C₈H₁₀N₂O₄
Molecular Weight	198.18	CAS No.	500-44-7
Solubility (25°C)*	In vitro	5%TFA	3 mg/mL (15.13 mM)
		DMSO	0.1 mg/mL (0.5 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	L-Mimosine (Leucenol) is a non-protein amino acid, and acts as an iron chelator.
In vitro	L-Mimosine arrests the cell cycle at the G1 phase in PC-3 cells and at the S phase in LNCaP cells, thus attenuating cell proliferation. ^[1] L-Mimosine, as a prolyl hydroxylase (PHD) inhibitor, stimulates VEGF production, and increases their proangiogenic capacity in dental pulp-derived cells. ^[2]
In vivo	L-mimosine (50 mg/kg i.p.) substantially upregulates HIF-1alpha expression in the kidneys, and remarkably enhances the natriuretic response to renal perfusion pressure in Sprague-Dawley rats. ^[3]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines PC-3 and LNCaP cells Concentrations ~800 μM Incubation Time 48 hours Method Cell proliferation in response to L-mimosine is measured using a [³H]thymidine incorporation assay. In this assay, 1 × 10⁴ cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [³H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO₂ atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate.
Animal Study:^{^[3]}	Animal Models Sprague-Dawley rats Dosages ~50 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
PC-3 and LNCaP cells
Concentrations
~800 μM
Incubation Time
48 hours
Method
Cell proliferation in response to L-mimosine is measured using a [³H]thymidine incorporation assay. In this assay, 1 × 10⁴ cells are cultured in each well of a 12-well plate in RPMI-1640 medium with 10% FCS and different concentrations (0–800 μM) of L-mimosine. After the required incubation periods (24 and 48 h), 0.5 μCi/ml of [³H]thymidine is added to each well of the 12-well plate. The cells are then incubated at 37°C in a humidified 5% CO₂ atmosphere for 4 h. Cells are then washed twice with cold phosphate-buffered saline (PBS) and then with cold 5% trichloroacetic acid. Cells are lysed by adding 0.5 ml of 0.5 N NaOH. Then 400 μl of the solubilized cell solution are mixed with 4 ml of scintillation cocktail and counted in a liquid scintillation analyzer . Each sample was tested in quadruplicate.

Animal Study:^{^[3]}

Animal Models
Sprague-Dawley rats
Dosages
~50 mg/kg
Administration
i.p.

References

Selleck's L-Mimosine has been cited by 5 publications

DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
DNA nicks induce mutational signatures associated with BRCA1 deficiency [ Nat Commun, 2022, 13(1):4285]	PubMed: 35879372
Identification of amitriptyline HCl, flavin adenine dinucleotide, azacitidine and calcitriol as repurposing drugs for influenza A H5N1 virus-induced lung injury. [ PLoS Pathog, 2020, 16;16(3):e1008341]	PubMed: 32176725
CCDC84 Acetylation Oscillation Regulates Centrosome Duplication by Modulating HsSAS-6 Degradation [ Cell Rep, 2019, 29(7):2078-2091.e5]	PubMed: 31722219
M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole. [ Nat Commun, 2018, 9(1):4511]	PubMed: 30375385

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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