Name: Hydroxytyrosol
Brand: Selleck Chemicals
SKU: S3826
Availability: InStock
Rating: 5 (4 reviews)

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Quality Control

Batch: Purity: 99.92%

99.92

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Solubility

In vitro
Batch:

DMSO : 31 mg/mL (201.08 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 31 mg/mL

Ethanol : 31 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 95 %Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.22mg/ml (1.43mM)	Taking the 1 mL working solution as an example, add 50 μL of 4.4 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.55mg/ml (10.05mM)	Taking the 1 mL working solution as an example, add 50 μL of 31 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	154.16	Formula	C₈H₁₀O₃	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	10597-60-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	3-Hydroxytyrosol; 3,4-dihydroxyphenylethanol; Dihydroxyphenylethanol; 2-(3,4-Di-hydroxyphenyl)-ethanol			SMILES	C1=CC(=C(C=C1CCO)O)O

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

In vitro	Hydroxytyrosol decreases the intracellular reactive oxygen species (ROS) level in MCF10A cells but not in MCF7 or MDA-MB-231 cells. This compound prevents oxidative DNA damage in the three breast cell lines: MCF10A, MDA-MB-231 and MCF7 cells. It is a potent antioxidant because of its marked antioxidant activity, its ability to scavenge oxygen and nitrogen free radicals, to inhibit Low Density Lipoprotein (LDL) oxidation, platelet aggregation and endothelial cell activation and its protection against DNA damage. It is able to reduce the synthesis of prostaglandin E2 blocking the transcription of COX-2 and 5-lipooxygenase, thereby reducing the chronic influence associated with diseases such as cancer.
In vivo	HT administration protects against hepatic I/R injury, as indicated by the decreased levels of serum aminotransferase and less parenchymal necrosis and apoptosis. Serum levels of tumor necrosis factor-α, interleukin-6, macrophage inflammatory protein 2, as well as reactive oxygen species (ROS) content in liver tissues, are all decreased by this compound.
References	[1] https://pubmed.ncbi.nlm.nih.gov/22254082/ [2] https://pubmed.ncbi.nlm.nih.gov/23650136/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-06-30)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07672938	RECRUITING	Mild Cognitive Impairment (MCI)	Panhellenic Federation of Alzheimer's Disease and Related Disorders	2025-03-31
NCT07689214	RECRUITING	Diabete Type 2	University of Roma La Sapienza	2024-07-17
NCT06833866	RECRUITING	Advanced or Metastatic CRC	The Methodist Hospital Research Institute	2024-12-04	PHASE1
NCT07298759	COMPLETED	Hypertension in Pregnancy	Dr Cipto Mangunkusumo General Hospital	2025-06-11
NCT06992323	ACTIVE_NOT_RECRUITING	Hypertriglyceridemia; Normal	National Research Council, Spain	2025-02-17
NCT07074548	COMPLETED	Hypertension in Pregnancy	Dr Cipto Mangunkusumo General Hospital	2025-07-10

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Hydroxytyrosol

Chemical Structure

Molecular Weight: 154.16