Pitstop 2

For research use only.

Catalog No.S9670

3 publications

Pitstop 2 Chemical Structure

CAS No. 1332879-52-3

Pitstop 2 is an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin that inhibits both clathrin-dependent endocytosis (CDE) and clathrin independent endocytosis (CIE).

Selleck's Pitstop 2 has been cited by 3 publications

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Biological Activity

Description Pitstop 2 is an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin that inhibits both clathrin-dependent endocytosis (CDE) and clathrin independent endocytosis (CIE).
Targets
CIE [1]
()
In vitro

Pitstop 2 is an inhibitor of the interaction of amphiphysin with the amino terminal domain of clathrin and inhibits clathrin-dependent endocytosis (CDE) and clathrin independent endocytosis (CIE) in cells. The effects of pitstop 2 are not restricted to inhibition of clathrin since knockdown of clathrin fails to rescue the inhibition of endocytosis of CIE proteins by the drug. Thus pitstop 2 has additional cellular targets besides the amino terminal domain of clathrin and thus cannot be used to distinguish CIE from CDE.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: Hela cells
  • Concentrations: 20 μM
  • Incubation Time: 15 min
  • Method:

    Hela cells are preincubated with DMSO (control) or 20 μM pitstop 2 or pitstop 2-negative control for 15 min. Cells are allowed to internalize Alexa594-Transferrin and antibodies to MHCI for 30 min at 37℃ in the presence of DMSO or drugs. Surface bound antibodies are removed by low pH wash to visualize the internalized proteins (Internal) prior to fixation or cells are fixed immediately after the internalization to label for the internal and surface pools (Total) of the protein. Cells are then labeled with secondary antibodies to detect MHCI.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 95 mg/mL (200.69 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 473.36
Formula

C20H13BrN2O3S2

CAS No. 1332879-52-3
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID