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Ethidium bromide

Name: Ethidium bromide
Brand: Selleck Chemicals
SKU: S3689
Rating: 5 (5 reviews)

Synonyms: Homidium bromide, EtBr, EB

Catalog No.S3689 Purity: 99.73%

Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

Ethidium bromide Chemical Structure

CAS: 1239-45-8

Selleck's Ethidium bromide has been cited by 5 publications

Purity & Quality Control

Batch: Purity: 99.73%

99.73

Ethidium bromide Related Products

Biological Activity

Description	Ethidium Bromide (Homidium bromide, EtBr, EB) is an intercalating agent which resembles a DNA base pair and commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis.

In vitro
In vitro	Ethidium Bromide (EtBr) is sometimes added to running buffer during the separation of DNA fragments by agarose gel electrophoresis. It is used because upon binding of the molecule to the DNA and illumination with a UV light source, the DNA banding pattern can be visualized. The mode of binding of EtBr is intercalation between the base pairs. This binding changes the charge, weight, conformation, and flexibility of the DNA molecule. The mobility of DNA was always less in the gels with EtBr^[1].

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT06007235	Not yet recruiting	Epidermolysis Bullosa Dystrophica\|Epidermolysis Bullosa Junctional	Organ Tissue Regeneration Repair and Replacement\|Euraxi Pharma\|ARANZ Medical	December 2023	Not Applicable
NCT06073132	Not yet recruiting	Generalized Epidermolysis Bullosa Simplex	TWi Biotechnology Inc.	December 2023	Phase 2\|Phase 3
NCT05557890	Recruiting	Psychological and Systems Functioning	University of Witten/Herdecke\|FH Münster University of Applied Sciences\|The Witten Institute For Family Business	November 1 2023	Not Applicable
NCT05691244	Not yet recruiting	Acute Ischemic Stroke\|Cerebral Stroke	Pharmazz Inc.	November 2023	Phase 3
NCT05955326	Not yet recruiting	Acute Cerebral Ischemia	Pharmazz Inc.	November 2023	Phase 4

References

[1] Sigmon J, et al. Electrophoresis. 1996, 17(10):1524-7.

Chemical Information & Solubility

Molecular Weight	394.31	Formula	C₂₁H₂₀N₃.Br
CAS No.	1239-45-8	SDF	Download Ethidium bromide SDF
Smiles	CC[N+]1=C2C=C(C=CC2=C3C=CC(=CC3=C1C4=CC=CC=C4)N)N.[Br-]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 78 mg/mL ( (197.81 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 7 mg/mL

Ethanol : 1 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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