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Methyl-β-cyclodextrin (MβCD)

Name: Methyl-β-cyclodextrin (MβCD)
Brand: Selleck Chemicals
SKU: S6827
Availability: InStock
Rating: 5 (13 reviews)

Cat.No.S6827

Methyl-β-cyclodextrin (MβCD) is a cholesterol-removing agent mainly used for lipid raft disruption. It induces caspase-dependent apoptosis in PEL cells and inhibits the growth and invasion of PEL cells without apparent adverse effects, suggesting the potential effective antitumor activities.

Chemical Structure

Molecular Weight:

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S682701 Purity: 98%

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Chemical Information, Storage & Stability

Molecular Weight		Formula		Storage (From the date of receipt)	3 years -20°C powder
CAS No.	128446-36-6	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Solubility

In vitro
Batch:

Water : 100 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	cholesterol ^[1] lipid raft ^[1]
In vitro	Methyl-β-cyclodextrin (MβCD) inhibits SDF-1α-induced platelet aggregation and Akt phosphorylation by raft disruption.^[2]
In vivo	In a Primary effusion lymphoma (PEL) xenograft mouse model, Methyl-β-cyclodextrin (MβCD) significantly inhibits the growth and invasion of PEL cells without apparent adverse effects. Mice treated with this compound appear to be healthy, whereas non-treated mice have a distended abdominal region. The body weights of control are significantly higher than those of the treated mice. It also results in a significantly lower volume of ascites than that of non-treated mice.^[1]
References	https://pubmed.ncbi.nlm.nih.gov/25446086/ https://pubmed.ncbi.nlm.nih.gov/28072855/

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