BAPTA-AM

Catalog No.S7534

BAPTA-AM Chemical Structure

Molecular Weight(MW): 764.68

BAPTA-AM is a selective, membrane-permeable calcium chelator.

Size Price Stock Quantity  
USD 97 In stock
USD 339 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 17 Publications

6 Customer Reviews

  • (A) Representative fluorescent images of co-localization of NLRP3 and mitochondria in NP cells as described in Figure 6C. Red and green fluorescence indicated NLRP3 and mitochondria signals, respectively. Cell nuclei are stained by DAPI. Representative fluorescent images of caspase-1 activity (B) in NP cells.

    J Cell Mol Med, 2017, 21(7):1373-1387. BAPTA-AM purchased from Selleck.

  • BAPTA/AM and STO609 pretreatments did not inhibit BAM15-induced AMPK activation in A10 cells. A10 cells were treated with BAM15 (5 μmol/L, 5min) after BAPTA-AM (20 μmol/L, 1h) and STO-609 (50 μmol/L, 1 h) pretreatments. **P<0.01 vs DMSO (control).

    Acta Pharm Sin B, 2018, 8(6):909-918. BAPTA-AM purchased from Selleck.

  • Formation of the AnxA2 complex in MBMECs was evaluated by immunoprecipitation and Western blot analysis after cryptococcal exposure. Endothelial cells were treated with PP2, BAPTA-AM, or DMSO (control) for 1 h before infection with cryptococcal cells and then rinsed twice with warm PBS. Lysates of infected or noninfected endothelial cells were preincubated with anti-AnxA2 Ab, and bound proteins were then blotted with anti-S100A10 Ab.

    CNS Neurosci Ther, 2017, 23(4):291-300. BAPTA-AM purchased from Selleck.

  • Calcium chelator (BAPTA-AM) reduces HBV replication in CD36OE of HepG2.2.15 and HepAD38 cells. BAPTA-AM or vehicle controls (DMSO) were added to the CD36OE of HepG2.2.15 and HepAD38 cells for 96 h, and HBV DNA were extracted and detected by Real-time PCR (n = 4). Cytosolic Ca2+ was measured after treatment for 48 h using flow cytometry (n = 3).

    Exp Cell Res, 2017, 358(2):360-368. BAPTA-AM purchased from Selleck.

  • A549 lung cancer cells were serum starved prior to treatment with TGF-β1 (10 ng/mL) or control for 12h in the presence or absence of BAPTA-AM (100μM). Protein expressions of Smad2/3, PSmad2 and P-Smad2 were detected by western blotting. (Values are shown as the mean ± SD. n=3, a representative experiment is shown; ‘*’means P<0.05)

    Int J Biochem Cell Biol, 2017, 90:103-113. BAPTA-AM purchased from Selleck.

  • ER stress is necessary for MHBs-induced p38/NF-κB activation and an increase in IL-6. Huh7, L-02 and SMMC-7721 cells were transfected with MHB or GFP, the cells were treated with BAPTA-AM. (a) The phosphorylation of p38 MAPK and degradation of Iκ-B were detected by western blot.

    PLoS One, 2016, 11(7):e0159089.. BAPTA-AM purchased from Selleck.

Purity & Quality Control

Biological Activity

Description BAPTA-AM is a selective, membrane-permeable calcium chelator.
In vitro

BAPTA-AM, as an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. [1] BAPTA-AM exerts an open channel blocking effect on hERG, hKv1.3 and hKv1.5 channels with IC50 of 1.3, 1.45 and 1.23 μM in HEK 293 cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SKOV3 MnnDSpVv[3Srb36gZZN{[Xl? M4\CV|EzKGh? NEH3[|J1emWjdH3lcpQhf2m2aDDCRXBVSS2DTTDy[YR2[2WmIITo[UBt\X[nbIOgc4YhY0OjMjvdbS=> Ml3uN|A5OTl{M{C=
OVCAR3 NVLPS5QzTnWwY4Tpc44h[XO|YYm= M1TLclEzKGh? M2DIVJRz\WG2bXXueEB4cXSqIFLBVHRCNUGPIILl[JVk\WRidHjlJIxmfmWuczDv[kBcS2F{K23p NUjBeWhzOzB6MUmyN|A>
MC3T3-E1 cells MWPGeY5kfGmxbjDhd5NigQ>? NYLI[lY{OsLizszN MnzFNlQhcA>? MUjCRXBVSS:DTTDheJRmdnWjdHXkJHRoMHSqYYDzbYdiemerbjmtbY5lfWOnZDDORWRRUCCxeHnkZZNmKGGldHn2bZR6 NUXrVHQzOjd2NUewO|U>
HUVECs Mn;ZSpVv[3Srb36gZZN{[Xl? NELOUFM2KM7:bX;sM2w> NVvLV49rOyCq M4L3UY1iemunZHz5JJN2eHC{ZYPz[YQhS2F{KzDpcoNz\WG|ZR?= NVLjflVUOjV4NEOxOFc>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p-ERK / ERK ; 

PubMed: 23185493     


Effects of BAPTA-AM (20 µM, 30 min) on SKF83959 (30 µM, 10 min)-induced ERK1/2 phosphorylation. 

23185493
In vivo BAPTA-AM abolishes the UDCA- and TUDCA-induced increases in Ca2+ levels or Ca2+-Dependent PKC-α phosphorylation, and neutralizes the cytoprotective effects of UDCA and TUDCA on the vagotomy-induced damage of the biliary tree in the BDL rats. [3]

Protocol

Animal Research:[3]
- Collapse
  • Animal Models: BDL rats
  • Formulation: 0.1% DMSO
  • Dosages: ~6 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 20 mg/mL (26.15 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 764.68
Formula

C34H40N2O18

CAS No. 126150-97-8
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Related Others Products

Tags: buy BAPTA-AM | BAPTA-AM supplier | purchase BAPTA-AM | BAPTA-AM cost | BAPTA-AM manufacturer | order BAPTA-AM | BAPTA-AM distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID