BAPTA-AM

Catalog No.S7534

For research use only.

BAPTA-AM is a selective, membrane-permeable calcium chelator.

BAPTA-AM Chemical Structure

CAS No. 126150-97-8

Selleck's BAPTA-AM has been cited by 51 publications

Purity & Quality Control

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Biological Activity

Description BAPTA-AM is a selective, membrane-permeable calcium chelator.
In vitro

BAPTA-AM, as an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. [1] BAPTA-AM exerts an open channel blocking effect on hERG, hKv1.3 and hKv1.5 channels with IC50 of 1.3, 1.45 and 1.23 μM in HEK 293 cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SKOV3 MX7GeY5kfGmxbjDhd5NigQ>? NWDZNXBYOTJiaB?= NH3ZZY11emWjdH3lcpQhf2m2aDDCRXBVSS2DTTDy[YR2[2WmIITo[UBt\X[nbIOgc4YhY0OjMjvdbS=> M4fkTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzNyOEG5NlMxLz5|MEixPVI{ODxxYU6=
OVCAR3 MV\GeY5kfGmxbjDhd5NigQ>? NGi4cIIyOiCq NUHzN5pifHKnYYTt[Y51KHerdHigRmFRXEFvQV2gdoVlfWOnZDD0bIUhdGW4ZXzzJI9nKFuFYUKrYYk> NHnrOlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9|MEixPVI{OCd-M{C4NVkzOzB:L3G+
MC3T3-E1 cells NH2w[ZJHfW6ldHnvckBie3OjeR?= MnTPNuKh|ryP MXiyOEBp Ml\3RmFRXEFxQV2gZZR1\W63YYTl[EBV\yi2aHHwd4lo[XKpaX6pMYlv\HWlZXSgUmFFWEhib4jp[IF{\SCjY4Tpeol1gQ>? NXriTJRURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMke0OVcxPzVpPkK3OFU4ODd3PD;hQi=>
HUVECs NFXBO4hHfW6ldHnvckBie3OjeR?= MUG1JO69dW:uL1y= NFTTUVg{KGh? NYHsWnpXdWG{a3XkcJkhe3WycILld5Nm\CCFYUKrJIlv[3KnYYPl MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ2M{G0O{c,OjV4NEOxOFc9N2F-
Assay
Methods Test Index PMID
Western blot p-ERK / ERK 23185493
In vivo BAPTA-AM abolishes the UDCA- and TUDCA-induced increases in Ca2+ levels or Ca2+-Dependent PKC-α phosphorylation, and neutralizes the cytoprotective effects of UDCA and TUDCA on the vagotomy-induced damage of the biliary tree in the BDL rats. [3]

Protocol (from reference)

Animal Research:[3]
  • Animal Models: BDL rats
  • Dosages: ~6 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 20 mg/mL
(26.15 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 764.68
Formula

C34H40N2O18

CAS No. 126150-97-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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