BAPTA-AM

BAPTA-AM is a selective, membrane-permeable calcium chelator.

BAPTA-AM Chemical Structure

BAPTA-AM Chemical Structure

CAS: 126150-97-8

Selleck's BAPTA-AM has been cited by 69 publications

Purity & Quality Control

Batch: Purity: 99.34%
99.34

BAPTA-AM Related Products

Cell Data

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
SKOV3 Function assay 12 h treatment with BAPTA-AM reduced the levels of [Ca2+]i 30819230
OVCAR3 Function assay 12 h treatment with BAPTA-AM reduced the levels of [Ca2+]i 30819230
MC3T3-E1 cells Function assay 2 μM 24 h BAPTA/AM attenuated Tg(thapsigargin)-induced NADPH oxidase activity 27457075
HUVECs Function assay 5 μmol/L 3 h markedly suppressed Ca2+ increase 25643147
Click to View More Cell Line Experimental Data

Biological Activity

Description BAPTA-AM is a selective, membrane-permeable calcium chelator.
In vitro
In vitro

BAPTA-AM, as an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. [1]

BAPTA-AM exerts an open channel blocking effect on hERG, hKv1.3 and hKv1.5 channels with IC50 of 1.3, 1.45 and 1.23 μM in HEK 293 cells. [2]

Experimental Result Images Methods Biomarkers Images PMID
Western blot p-ERK / ERK 23185493
In Vivo
In vivo

BAPTA-AM abolishes the UDCA- and TUDCA-induced increases in Ca2+ levels or Ca2+-Dependent PKC-α phosphorylation, and neutralizes the cytoprotective effects of UDCA and TUDCA on the vagotomy-induced damage of the biliary tree in the BDL rats. [3]

Animal Research Animal Models BDL rats
Dosages ~6 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 764.68 Formula

C34H40N2O18

CAS No. 126150-97-8 SDF Download BAPTA-AM SDF
Smiles CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 20 mg/mL ( (26.15 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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