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BAPTA-AM

Name: BAPTA-AM
Brand: Selleck Chemicals
SKU: S7534
Availability: InStock
Rating: 5 (85 reviews)

Cat.No.S7534

BAPTA-AM is a selective, membrane-permeable calcium chelator.

Chemical Structure

Molecular Weight: 764.68

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.34%

99.34

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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
SKOV3	Function assay		12 h	treatment with BAPTA-AM reduced the levels of [Ca2+]i	30819230
OVCAR3	Function assay		12 h	treatment with BAPTA-AM reduced the levels of [Ca2+]i	30819230
MC3T3-E1 cells	Function assay	2 μM	24 h	BAPTA/AM attenuated Tg(thapsigargin)-induced NADPH oxidase activity	27457075
HUVECs	Function assay	5 μmol/L	3 h	markedly suppressed Ca2+ increase	25643147
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	764.68	Formula	C₃₄H₄₀N₂O₁₈	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	126150-97-8	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(=O)OCOC(=O)CN(CC(=O)OCOC(=O)C)C1=CC=CC=C1OCCOC2=CC=CC=C2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C

Solubility

In vitro
Batch:

DMSO : 20 mg/mL ( (26.15 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	BAPTA-AM, as an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. ^[1] This compound exerts an open channel blocking effect on hERG, hKv1.3 and hKv1.5 channels with IC50 of 1.3, 1.45 and 1.23 μM in HEK 293 cells. ^[2]
In vivo	BAPTA-AM abolishes the UDCA- and TUDCA-induced increases in Ca²⁺ levels or Ca2+-Dependent PKC-α phosphorylation, and neutralizes the cytoprotective effects of UDCA and TUDCA on the vagotomy-induced damage of the biliary tree in the BDL rats. ^[3]
References	https://pubmed.ncbi.nlm.nih.gov/11642660/ https://pubmed.ncbi.nlm.nih.gov/17826747/ https://pubmed.ncbi.nlm.nih.gov/16436655/ https://pubmed.ncbi.nlm.nih.gov/35696763/

Applications

Methods	Biomarkers	Images	PMID
Western blot	p-ERK / ERK		23185493

Tech Support

Handling Instructions

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