4-Hydroxynonenal

Catalog No.S9793 Synonyms: 4-HNE, HNE, 4-hydroxy Nonenal, 4-hydroxy-2-nonenal

For research use only.

4-Hydroxynonenal (4-HNE, HNE, 4-hydroxy Nonenal, 4-hydroxy-2-nonenal) is one of the major end products of lipid peroxidation and has been widely accepted as an inducer of oxidative stress.

4-Hydroxynonenal Chemical Structure

CAS No. 75899-68-2

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Biological Activity

Description

4-Hydroxynonenal (4-HNE, HNE, 4-hydroxy Nonenal, 4-hydroxy-2-nonenal) is one of the major end products of lipid peroxidation and has been widely accepted as an inducer of oxidative stress.

In vitro

4-Hydroxynonenal (4-HNE) is one of the main end products of lipid peroxidation,  which induces the expression of heme oxygenase-1 (HO-1), peroxiredoxin-1 and stress protein A170 in an Nrf2-dependent manner in murine macrophages.<sup><a class="sref" href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Research:

<sup><a class="sref" href="#s_ref">[2]</a></sup>

  • Cell lines: Mouse Peritoneal Macrophages
  • Concentrations: 10 μM
  • Incubation Time: 24 h
  • Method:

    Oil Red O Staining: Mouse peritoneal macrophages were incubated for 24 h at 37℃ in 5% CO2 in PRMI 1640 medium containing 10% FCS in the absence or presence of 4-Hydroxynonenal (4-HNE) (10 μM) or moxLDL or oxLDL (200 μg protein/ml). Cells were washed twice, fixed in formalin, and stained with oil red O. The area of lipid-loaded macrophages was measured using a computerized MacSCOPE image analysis system.

Chemical Information

Molecular Weight 156.22
Formula

C9H16O2

Density 0.9 g/mL
CAS No. 75899-68-2
Storage 2 years -20°C liquid

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05414370 Not yet recruiting Drug: Liquid oxygen|Drug: medical air Oxygen Toxicity|Pulmonary Injury|Acute Lung Injury Belfast Health and Social Care Trust August 2022 Not Applicable
NCT04979637 Not yet recruiting -- Wound Infection|Wound Heal ConvaTec Inc.|ClinSearch|Qualizyme Diagnostics GmbH & Co KG|Perfectus BioMed|Welsh Wound Innovation Centre|Maison de Santé Protestante de Bordeaux July 2021 --
NCT04259255 Recruiting Drug: Edaravone Amyotrophic Lateral Sclerosis|ALS Mitsubishi Tanabe Pharma America Inc.|Massachusetts General Hospital October 21 2019 --
NCT03576729 Completed -- Mucopolysaccharidosis Type I University of Minnesota|Rare Diseases Clinical Research Network|National Center for Advancing Translational Science (NCATS)|National Institute of Neurological Disorders and Stroke (NINDS)|National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)|Lysosomal Disease Network November 1 2018 --

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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