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4-HNE (4-Hydroxynonenal) Oxidative Stress Inducer

Name: 4-HNE (4-Hydroxynonenal)
Brand: Selleck Chemicals
SKU: S9793
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S9793

4-HNE (4-Hydroxynonenal) is one of the major end products of lipid peroxidation and has been widely accepted as an inducer of oxidative stress.

Chemical Structure

Molecular Weight: 156.22

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Quality Control

Batch: Purity: 99.55%

99.55

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Endogenous Metabolite Inhibitors	Heparan Sulfate Ademetionine (S-Adenosylmethionine) Sodium L-lactate

Solubility

In vitro
Batch:

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (32.01mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.830mg/ml (5.31mM)	Taking the 1 mL working solution as an example, add 50 μL of 16.6 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	156.22	Formula	C₉H₁₆O₂	Storage (From the date of receipt)	2 years -20°C liquid
CAS No.	75899-68-2	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Mechanism of Action

In vitro	4-Hydroxynonenal (4-HNE) is one of the main end products of lipid peroxidation, which induces the expression of heme oxygenase-1 (HO-1), peroxiredoxin-1 and stress protein A170 in an Nrf2-dependent manner in murine macrophages.<sup><a class="sref" href="#s_ref">[2]</a></sup>
References	[1] https://pubmed.ncbi.nlm.nih.gov/16754333/ [2] https://pubmed.ncbi.nlm.nih.gov/14752028/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06126315	Not yet recruiting	Amyotrophic Lateral Sclerosis	Mario Negri Institute for Pharmacological Research\|University of Sydney\|FightMND	June 2024	Phase 2\|Phase 3
NCT05742698	Active not recruiting	Frontotemporal Dementia\|Frontotemporal Dementia Behavioral Variant\|Primary Progressive Aphasia\|bvFTD\|PPA\|FTD	Simon Ducharme MD\|Alzheimer''s Drug Discovery Foundation\|Douglas Mental Health University Institute	March 7 2023	Phase 2
NCT05829382	Active not recruiting	Healthy Aging	Helse Stavanger HF	March 15 2023	Not Applicable

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Handling Instructions

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