Name: Blebbistatin
Brand: Selleck Chemicals
SKU: E1249
Rating: 5 (10 reviews)

Blebbistatin is a selective inhibitor of non-muscle myosin II (NMII). It also potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. It promotes directional migration of corneal endothelial cells (CECs) and accelerates wound healing, and better preserves cell junctional integrity and barrier function.

Blebbistatin Chemical Structure

CAS: 674289-55-5

Selleck's Blebbistatin has been cited by 10 publications

Purity & Quality Control

Batch: E124901 DMSO] 58 mg/mL] false] Ethanol] 5 mg/mL] false] Water] Insoluble] false Purity: 99.39%

99.39

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Biological Activity

Description

Blebbistatin is a selective inhibitor of non-muscle myosin II (NMII). It also potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. It promotes directional migration of corneal endothelial cells (CECs) and accelerates wound healing, and better preserves cell junctional integrity and barrier function.

References

Chemical Information & Solubility

Molecular Weight	292.33	Formula	C₁₈H₁₆N₂O₂
CAS No.	674289-55-5	SDF	--
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 58 mg/mL ( (198.4 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 5 mg/mL

Water : Insoluble

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In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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