Palmitic acid

Catalog No.S3794 Synonyms: hexadecanoic acid, Cetylic acid

For research use only.

Palmitic acid (Hexadecanoic acid, Cetylic acid) is the most common saturated fatty acid found in animals, plants and microorganisms with anti-tumor activity.

Palmitic acid Chemical Structure

CAS No. 57-10-3

Selleck's Palmitic acid has been cited by 2 Publications

Purity & Quality Control

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Biological Activity

Description Palmitic acid (Hexadecanoic acid, Cetylic acid) is the most common saturated fatty acid found in animals, plants and microorganisms with anti-tumor activity.
In vitro

Palmitic acid (PA) binds TLR4 via the adaptor protein MD2 and that the TLR4 signal induced by PA stimulates dendritic cells activation and maturation accompanied by a robust IL-1β response. Palmitic acid is a TLR4/MD-2 ligand. It exerts pro-inflammatory activity through TLR4[1].

In vivo In a dose-dependent fashion, palmitic acid rapidly reduces mouse locomotor activity by a mechanism that did not rely on TLR4, MyD88, IL-1, IL-6 or TNFα but is dependent on fatty acid chain length. Palmitic acid induces anxiety-like behavior in mice while increasing amygdala-based serotonin metabolism[2].

Protocol (from reference)

Cell Research:[3]
  • Cell lines: The insulinoma cell line INS-1
  • Concentrations: 250 μmol/L
  • Incubation Time: 24 or 48 h
  • Method: The INS-1 cells are seeded onto 96-well plates at 4,000 cells/well with 10% BSA and incubated with T0901317 (10 μmol/L), palmitic acid (250 μmol/L), a combination of T0901317 and palmitic acid or PBS (mock control) for 24 or 48 h. After the treatment incubation, 20 μl of MTT solution (5 mg/mL MTT in PBS, pH 7.4) are added to each well, followed by incubation at 37°C for 4 h. The supernatants are removed from the wells and DMSO (15 μl/well) is added to dissolve the crystals. The optical density at a wavelength of 490 nm is measured using a microplate reader.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: C57BL/6J mice
  • Dosages: 0.3, 3, and 30 μmol/mouse
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 51 mg/mL
(198.89 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 256.42


CAS No. 57-10-3
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04541095 Active not recruiting Other: EX_IMF|Other: ST_IMF Weight Gain Ospedali Riuniti Ancona|Istituto di Ricerca Pediatrica Città della Speranza|Bunge Loders Croklaan September 1 2020 Not Applicable
NCT03665922 Recruiting Dietary Supplement: BroccoMax®|Other: Placebo Prostate Cancer University of Pittsburgh May 1 2019 Not Applicable
NCT04832217 Completed Other: Pyrenees´ Beef diet Body Weight Changes|Habits Diet|Habit Food|Life Change Events Universidad de Zaragoza January 20 2019 Not Applicable
NCT02502578 Completed Drug: CNT-01 500 mg capsule Idiopathic Triglyceride Deposit Cardiomyovasculopathy Osaka University September 2015 Phase 1|Phase 2

(data from, updated on 2021-09-06)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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