Name: DTT (Dithiothreitol)
Brand: Selleck Chemicals
SKU: S6829
Availability: InStock
Rating: 5 (2 reviews)

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Quality Control

Batch: Purity: 99%

99

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Solubility

In vitro
Batch:

DMSO : 31 mg/mL (200.97 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 31 mg/mL

Ethanol : 31 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.550mg/ml (10.05mM)	Taking the 1 mL working solution as an example, add 50 μL of 31 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.250mg/ml (1.62mM)	Taking the 1 mL working solution as an example, add 50 μL of 5 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	154.25	Formula	C₄H₁₀O₂S₂	Storage (From the date of receipt)	3 years -20°C powder (seal)
CAS No.	3483-12-3	--		Storage of Stock Solutions	Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.
Synonyms	Cleland's reagent			SMILES	C(C(C(CS)O)O)S

Read more about storage stability CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	disulfide bond
References	[1] https://pubmed.ncbi.nlm.nih.gov/22353145/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-06-25)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07660978	NOT_YET_RECRUITING	PARKINSON DISEASE (Disorder); Parkinson Disease (PD); Parkinson s Disease; Postural Instability; Postural Instability Gait Disorders; Gait Disorders; Cognitive Dysfunction, Cognitive Disorder	Bezmialem Vakif University	2026-06-20
NCT03883139	ACTIVE_NOT_RECRUITING	Autism Spectrum Disorder	University of California, Los Angeles	2021-03-01
NCT07209982	COMPLETED	Hemiplegia; Hemiplegia Following Ischemic Stroke; Stroke	University of Lahore	2025-01-15
NCT04057976	COMPLETED	Vestibular Schwannoma	King's College Hospital NHS Trust	2019-06-27
NCT05730790	UNKNOWN	Mild Cognitive Impairment	Tan Tock Seng Hospital	2021-03-31
NCT06255119	Completed	Opioid Use Disorder	3D Therapy LLC	2022-10-18	Not Applicable

Tech Support

Handling Instructions

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DTT (Dithiothreitol)

Chemical Structure

Molecular Weight: 154.25