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GSK4112

Name: GSK4112
Brand: Selleck Chemicals
SKU: S5182
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S5182

GSK4112 is a functional Rev-erbα agonist with an EC50 of 0.4 μM. It represses the expression of gluconeogenic genes in liver cells and reduces glucose output in primary hepatocytes.

Chemical Structure

Molecular Weight: 396.89

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Quality Control

Batch: S518201 DMSO]68 mg/mL]false]Ethanol]3 mg/mL]false]Water]Insoluble]false Purity: 99.66%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS
Datasheet

99.66

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Others Inhibitors	BAY 2927088（Sevabertinib） Guanidinoacetic acid BAPTA-AM Blebbistatin Methyl-β-cyclodextrin (MβCD) Carbazochrome sodium sulfonate (AC-17) Ursolic Acid Osthole 4-Methylumbelliferone (4-MU) Dioscin

Solubility

In vitro
Batch:

DMSO : 68 mg/mL (171.33 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml (6.30mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	396.89	Formula	C₁₈H₂₁ClN₂O₄S	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1216744-19-2	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	CC(C)(C)OC(=O)CN(CC1=CC=C(C=C1)Cl)CC2=CC=C(S2)[N+](=O)[O-]

Mechanism of Action

Targets/IC₅₀/Ki	Rev-erbα (Rev-erbα/NCoR ID1 FRET assay) 0.4 μM(EC50)
In vitro	In cells, GSK4112 profiles as a Rev-erbα agonist to inhibit expression of the circadian target gene bmal1. This compound suppresses the expression of gluconeogenic genes in liver cells and reduces glucose output in primary hepatocytes.
References	[1] https://pubmed.ncbi.nlm.nih.gov/20677822/

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