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Guanidine HCl

Name: Guanidine HCl
Brand: Selleck Chemicals
SKU: S4070
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.S4070

Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.

Chemical Structure

Molecular Weight: 95.53

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S407001 DMSO]19 mg/mL]false]Water]19 mg/mL]false]Ethanol]6 mg/mL]false Purity: 100.00%

100.00

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Chemical Information, Storage & Stability

Molecular Weight	95.53	Formula	CH₅N₃.HCl	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	50-01-1	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Aminoformamidine HCl			Smiles	C(=N)(N)N.Cl

Solubility

In vitro
Batch:

DMSO : 19 mg/mL (198.89 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 19 mg/mL

Ethanol : 6 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro

Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by this compound provides several important parameters regarding the mechanism of conformational stability of proteins. ^[1] It at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. This chemical (> 1 M) causes co-operative unfolding of the molten globule state. ^[2] It is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △G^H₂O_app. values of this compound are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. ^[3] It at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI⁺] element from yeast cells. 5 mM of this chemical in growth media cures [PSI⁺] and other prions of yeast. ^[4] 5 mM of this compound significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. It also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. ^[5]

References

[4] http://www.ncbi.nlm.nih.gov/pmc/articles/PMC26647/
[5] https://pubmed.ncbi.nlm.nih.gov/11375656/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03658902	Completed	Botulism; Poisoning	Centre Hospitalier le Mans	January 1 2000	--

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