Catalog No.S4070 Synonyms: Aminoformamidine HCl
Molecular Weight(MW): 95.53
Guanidine HCl, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.
Purity & Quality Control
|Description||Guanidine HCl, the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.|
Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by Guanidine HCl provides several important parameters regarding the mechanism of conformational stability of proteins.  Guanidine HCl at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. Guanidine HCl (> 1 M) causes co-operative unfolding of the molten globule state.  Guanidine HCl is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △GH2Oapp. values of Guanidine HCl are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2.  Guanidine HCl at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM Guanidine HCl in growth media cures [PSI+] and other prions of yeast.  5 mM Guanidine HCl significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. Guanidine HCl also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase. 
-  Pace CN. Methods Enzymol, 1986, 131, 266-280.
-  Hagihara Y, et al. J Mol Biol, 1993, 231(2), 180-184.
-  Greene RF Jr, et al. J Biol Chem, 1974, 249(17), 5388-5393.
|In vitro||DMSO||19 mg/mL (198.89 mM)|
|Water||19 mg/mL (198.89 mM)|
|Ethanol||6 mg/mL (62.8 mM)|
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