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Guanidine HCl

Cat.No.S4070

Guanidine HCl (Aminoformamidine), the crystalline compound of strong alkalinity formed by the oxidation of guanine, is a normal product of protein metabolism and a protein denaturant.
Guanidine HCl Chemical Structure

Chemical Structure

Molecular Weight: 95.53

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Quality Control

Batch: S407001 DMSO]19 mg/mL]false]Water]19 mg/mL]false]Ethanol]6 mg/mL]false Purity: 100.00%
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100.00

Solubility

In vitro
Batch:

DMSO : 19 mg/mL (198.89 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 19 mg/mL

Ethanol : 6 mg/mL

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In vivo
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In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Chemical Information, Storage & Stability

Molecular Weight 95.53 Formula

CH5N3.HCl

 Storage (From the date of receipt)
CAS No. 50-01-1 Download SDF Storage of Stock Solutions

Synonyms Aminoformamidine HCl Smiles C(=N)(N)N.Cl

Mechanism of Action

In vitro
Guanidine HCl is the most popular protein denaturant. Analysis of unfolding transitions by this compound provides several important parameters regarding the mechanism of conformational stability of proteins. It at low concentrations refolds acid-unfolds apomyoglobin and cytochrome c, stabilizing the molten globule state. This chemical (> 1 M) causes co-operative unfolding of the molten globule state. It is 2.8 times more effective than urea in unfolding ribonuclease but only 1.7 times more effective for lysozyme. △GH2Oapp. values of this compound are 9.7 Cal/mole for ribonuclease at pH 6.6, 6.1 for lysozyme at pH 2.9, 8.3 for α-chymotrypsin at pH 4.3, and 11.7 for β-lactoglobulin at pH 3.2. It at millimolar concentrations, is able to causes efficient loss of the normally stable [PSI+] element from yeast cells. 5 mM of this chemical in growth media cures [PSI+] and other prions of yeast. 5 mM of this compound significantly reduces Hsp104-mediated basal and acquired thermotolerance by 30-fold and 50 fold, respectively. It also reduces the ability of Hsp104 to restore activity of thermally denatured luciferase.
References
  • [4] http://www.ncbi.nlm.nih.gov/pmc/articles/PMC26647/
  • [5] https://pubmed.ncbi.nlm.nih.gov/11375656/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03658902 Completed
Botulism; Poisoning
Centre Hospitalier le Mans
 January 1 2000 --

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