Iodoacetamide

For research use only.

Catalog No.S9827 Synonyms: IA, IAM, 2-Iodoacetamide

2 publications

Iodoacetamide Chemical Structure

CAS No. 144-48-9

Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.

Selleck's Iodoacetamide has been cited by 2 publications

Purity & Quality Control

Biological Activity

Description Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.
In vitro

A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of iodoacetamide.[2]

In vivo

Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). Iodoacetamide induces gastritis in rats.[3]

Protocol

Cell Research:

[2]

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  • Cell lines: Drosophila melanogaster cells
  • Concentrations: 2 mM
  • Incubation Time: 15 mins
  • Method:

    Infected cells pretreated with 2 mM iodoaeetamide for 15 minutes before pulsing with 500 μCi of 35S-methionine for 30 minutes. The molecular weights are calculated by direct comparison of the stained dried slab gel and the corresponding autoradiogram.


    (Only for Reference)
Animal Research:

[3]

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  • Animal Models: Wistar rats, 8-week-old male CD1 and C57Bl/6J mice
  • Dosages: 0.05%, 0.1%, 0.2%, 0.3% and 0.5% concentration
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 40 mg/mL (216.26 mM)
Water 40 mg/mL (216.26 mM)
Ethanol '''40 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 184.96
Formula

C2H4INO

CAS No. 144-48-9
Storage powder
in solvent
Synonyms IA, IAM, 2-Iodoacetamide

In vivo Formulation Calculator (Clear solution)

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Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03948984 Not yet recruiting Behavioral: Therapeutic Music Session Anxiety|Pain|Critical Illness|Relaxation Vanderbilt University Medical Center July 2022 Not Applicable
NCT05016960 Not yet recruiting Behavioral: Sleep-SMART Insomnia|Mild Cognitive Impairment VA Office of Research and Development January 4 2022 Not Applicable
NCT04999436 Not yet recruiting Behavioral: APOL1 Counseling Training Program Chronic Kidney Diseases|Genetic Predisposition Northwestern University|Georgetown University December 2021 Not Applicable
NCT04890548 Not yet recruiting Drug: AZD3427 Heart Failure AstraZeneca|Parexel October 4 2021 Phase 1
NCT04965363 Not yet recruiting -- Internet Addiction|Internet Gaming Disorder Assiut University October 1 2021 --

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID