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Iodoacetamide

Synonyms: IA, IAM, 2-Iodoacetamide

Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.

Iodoacetamide Chemical Structure

Iodoacetamide Chemical Structure

CAS: 144-48-9

Selleck's Iodoacetamide has been cited by 2 publications

Purity & Quality Control

Batch: S982701 DMSO] 40 mg/mL] false] Water] 40 mg/mL] false] Ethanol] 40 mg/mL] false Purity: >97%
97

Iodoacetamide Related Products

Biological Activity

Description Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.
In vitro
In vitro

A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of iodoacetamide.[2]
Cell Research Cell lines Drosophila melanogaster cells
Concentrations 2 mM
Incubation Time 15 mins
Method

Infected cells pretreated with 2 mM iodoaeetamide for 15 minutes before pulsing with 500 μCi of 35S-methionine for 30 minutes. The molecular weights are calculated by direct comparison of the stained dried slab gel and the corresponding autoradiogram.
In Vivo
In vivo

Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). Iodoacetamide induces gastritis in rats.[3]
Animal Research Animal Models Wistar rats, 8-week-old male CD1 and C57Bl/6J mice
Dosages 0.05%, 0.1%, 0.2%, 0.3% and 0.5% concentration
Administration Oral gavage
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05986214 Not yet recruiting
Chronic Obstructive Pulmonary Disease
VA Office of Research and Development
 March 1 2026 Not Applicable
NCT06188585 Not yet recruiting
Acute Gastrointestinal Bleeding
Medtronic - MITG|NAMSA
 May 2024 Not Applicable
NCT05884307 Not yet recruiting
Gender Dysphoria
University of Wisconsin Madison
 April 2024 Not Applicable

Chemical Information & Solubility

Molecular Weight 184.96 Formula

C2H4INO
CAS No. 144-48-9 SDF --
Smiles NC(=O)CI
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 40 mg/mL ( (216.26 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 40 mg/mL

Ethanol : 40 mg/mL


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In vivo
Batch:

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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