For research use only.

Catalog No.S9827 Synonyms: IA, IAM, 2-Iodoacetamide

2 publications

Iodoacetamide Chemical Structure

CAS No. 144-48-9

Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.

Selleck's Iodoacetamide has been cited by 2 publications

Purity & Quality Control

Biological Activity

Description Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.
In vitro

A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of iodoacetamide.[2]

In vivo

Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). Iodoacetamide induces gastritis in rats.[3]


Cell Research:


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  • Cell lines: Drosophila melanogaster cells
  • Concentrations: 2 mM
  • Incubation Time: 15 mins
  • Method:

    Infected cells pretreated with 2 mM iodoaeetamide for 15 minutes before pulsing with 500 μCi of 35S-methionine for 30 minutes. The molecular weights are calculated by direct comparison of the stained dried slab gel and the corresponding autoradiogram.

    (Only for Reference)
Animal Research:


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  • Animal Models: Wistar rats, 8-week-old male CD1 and C57Bl/6J mice
  • Dosages: 0.05%, 0.1%, 0.2%, 0.3% and 0.5% concentration
  • Administration: Oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 40 mg/mL (216.26 mM)
Water 40 mg/mL (216.26 mM)
Ethanol '''40 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 184.96


CAS No. 144-48-9
Storage powder
in solvent
Synonyms IA, IAM, 2-Iodoacetamide

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03948984 Not yet recruiting Behavioral: Therapeutic Music Session Anxiety|Pain|Critical Illness|Relaxation Vanderbilt University Medical Center July 2022 Not Applicable
NCT05016960 Not yet recruiting Behavioral: Sleep-SMART Insomnia|Mild Cognitive Impairment VA Office of Research and Development January 4 2022 Not Applicable
NCT04999436 Not yet recruiting Behavioral: APOL1 Counseling Training Program Chronic Kidney Diseases|Genetic Predisposition Northwestern University|Georgetown University December 2021 Not Applicable
NCT04890548 Not yet recruiting Drug: AZD3427 Heart Failure AstraZeneca|Parexel October 4 2021 Phase 1
NCT04965363 Not yet recruiting -- Internet Addiction|Internet Gaming Disorder Assiut University October 1 2021 --

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID