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Iodoacetamide

Name: Iodoacetamide
Brand: Selleck Chemicals
SKU: S9827
Availability: InStock
Rating: 5 (2 reviews)

Cat.No.S9827

Iodoacetamide (IA, IAM, 2-Iodoacetamide) is an alkylating agent that is commonly used for alkylation of cysteine during sample preparation for proteomics.

Chemical Structure

Molecular Weight: 184.96

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Purity: >97%

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Chemical Information, Storage & Stability

Molecular Weight	184.96	Formula	C₂H₄INO	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	144-48-9	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Solubility

In vitro
Batch:

DMSO : 40 mg/mL (216.26 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 40 mg/mL

Ethanol : 40 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.29mg/ml (1.57mM)	Taking the 1 mL working solution as an example, add 50 μL of 5.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2mg/ml (10.81mM)	Taking the 1 mL working solution as an example, add 50 μL of 40 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro	A multiple cleavage cascade mechanism is partially utilized to produce virus structural proteins. In infected cells, the largest viral induced protein obtained has a molecular weight of 144,000. Pretreatment of the infected cells with iodoacetamide before pulsing with 35S-methionine results in the appearance of two polypeptides of molecular weights 205,000 and 190,000. Other changes occur in viral protein precursors in the presence of this compound.^[2]
In vivo	Iodoacetamide induces a concentration-dependent weight loss and reduces water intake in both species (Wistar rats and CD1, C57Bl/6J mice). This compound induces gastritis in rats.^[3]
References	[1] https://pubmed.ncbi.nlm.nih.gov/28799334/ [2] https://pubmed.ncbi.nlm.nih.gov/6788024/ [3] https://pubmed.ncbi.nlm.nih.gov/32764237/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT05986214	Not yet recruiting	Chronic Obstructive Pulmonary Disease	VA Office of Research and Development	March 1 2026	Not Applicable
NCT06376253	Not yet recruiting	Ovarian Cancer\|Lung Cancer	Novartis Pharmaceuticals\|Novartis	September 6 2024	Phase 1
NCT06406309	Not yet recruiting	Attention Deficit Hyperactivity Disorder\|Sleep Disturbance	University of Pittsburgh\|The Klingenstein Third Generation Foundation	September 2024	Not Applicable

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