Uric Acid

Catalog No.S3955 Synonyms: 2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine

For research use only.

Uric Acid (2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine), a normal component of urine, is a product of the metabolic breakdown of purine nucleotides.

Uric Acid Chemical Structure

CAS No. 69-93-2

Purity & Quality Control

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Biological Activity

Description Uric Acid (2,6,8-Trioxypurine, 2,6,8-Trihydroxypurine, 2,6,8-Trioxopurine), a normal component of urine, is a product of the metabolic breakdown of purine nucleotides.
In vitro

Uric acid activates NFκB in a variety of cell culture models including proximal tubular cells. Uric acid suppresses 1-α hydroxylase mRNA and protein expression in dose dependent and time dependent manner[1].

In vivo Uric acid is synthesized mainly in the liver, intestines and the vascular endothelium as the end product of an exogenous pool of purines, and endogenously from damaged, dying and dead cells, whereby nucleic acids, adenine and guanine, are degraded into uric acid. Uric acid is a strong reactive oxygen species (ROS) and peroxynitrite scavenger and antioxidant. Uric acid may exert fundamental roles in tissue healing via initiating the inflammatory process that is necessary for tissue repair, scavenging oxygen free radicals, and mobilizing progenitor endothelial cells[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HK2 cells
  • Concentrations: 2.5, 5.0, 7.5, and 10 mg/dL
  • Incubation Time: 1, 2, 4, 8, 16, and 24 hours
  • Method: Cells are cultured in Keratinocyte-SFM basal media, supplemented with Bovine Pituitary Extract (20-30 μg/mL) and recombinant Epidermal Growth Factor (0.1-0.2 ng/mL), 5% Fetal Bovine Serum, 100 U/ml penicillin, and 10 g/ml streptomycin. Cells are cultured at 37°C in 95% air-5% CO2 until they were 90% confluent, and then allowed to differentiate for 5-7 days. After serum starvation for 24 hours, cells are stimulated with uric acid. For the dose response experiments, uric acid is given at 2.5, 5.0, 7.5, and 10 mg/dL and the cells are collected after 24 hours of treatment. Uric acid is used at 10 mg/dL for the time course experiments and the cells are collected at: 1, 2, 4, 8, 16, and 24 hours. To prevent uric acid crystal formation in the media, uric acid is dissolved in prewarmed media, and kept at 37°C for a minimum of 30 minutes prior to application in cell culture.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 0.01 mg/mL
(0.05 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 168.11
Formula

C5H4N4O3

CAS No. 69-93-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C12=C(NC(=O)N1)NC(=O)NC2=O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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