Methyl Dihydrojasmonate

Catalog No.S5139 Synonyms: Hedione, Kharismal

For research use only.

Methyl dihydrojasmonate (Hedione, Kharismal) is a flavouring ingredien with the smell vaguely similar to jasmin.

Methyl Dihydrojasmonate Chemical Structure

CAS No. 24851-98-7

Purity & Quality Control

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Biological Activity

Description Methyl dihydrojasmonate (Hedione, Kharismal) is a flavouring ingredien with the smell vaguely similar to jasmin.
In vitro

Methyl dihydrojasmonate inhibits the growth of leukemia cells as well as lung, breast, and prostate cancer cells. It opens the mitocondrial permeability transition-pore complex, allowing cytochrome C release and induction of apoptosis, and the expression of reactive oxygen species. Jasmonate-derivative compounds, including jasmonic acid (JA) and methyl dihydrojasmonate (MJ), also induces the suppression of cell proliferation in human and mouse cancer cell lines, such as prostate cancer and breast cancers, melanoma, leukemia, lymphoid, and myeloid[2].

In vivo Methyl dihydrojasmonate (MDHJ) is a promising anti-cancer drug of a plant origin (jasmine plant)[1]. Jasmonate-derivative compounds are poorly water-soluble, and cannot be administered intravenously or adequately absorbed by the body[2]. The oral LD50 of Methyl dihydrojasmonate in rats exceeds 5.0 g/kg. By the dermal route, the rabbit LD50 also exceeds 5.0 g/kg. By the intraperitoneal route in ICR mice, the LD50 is 1397.2 mg/kg. While in rats, an intraperitoneal injection of Methyl dihydrojasmonate in corn oil produces clinical signs at 1000 mg/kg and mortality at 2000 mg/kg. Methyl dihydrojasmonate is not considered to possess a mutagenic or genotoxic potential[3].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: MCF-7 cells
  • Concentrations: 0, 5, 12.5, 25, 50 μl/ml
  • Incubation Time: 48 h
  • Method:

    Measurement of potential cytotoxicity is performed by SRB assay where Cells are plated in 96-multiwell plate (104 cells/well) for 24 h before exposure to the compound(s) to allow attachment of the cells to the wall of the plate. Different concentrations of the compound under test; 0, 5, 12.5, 25, 50 μl/ml for the Pure MDHJ (Oily liquid) and 0, 25, 50, 75, 100 μl/ml for the used formulation are added to the cell monolayer triplicate wells; which are prepared for each individual concentration. A monolayer cells are incubated together with the compound(s) for 48 h at 37 °C and in atmosphere of 5% CO2. After 48 h, cells are fixed, washed and stained with Sulfo-Rhodamine-B stain (SRB). Excess stain is washed with acetic acid and the attached stain is recovered with Tris EDTA buffer. Color intensity is measured utilizing an ELISA reader.

  • (Only for Reference)
Animal Research:

[3]

  • Animal Models: Sprague-Dawley rats
  • Dosages: 0, 40, 80 or 120 mg/kg/day
  • Administration: oral
  • (Only for Reference)

Chemical Information

Molecular Weight 226.31
Formula

C13H22O3

Density 0.998 g/mL at 25 °C
CAS No. 24851-98-7
Storage 2 years 4°C liquid
Smiles CCCCCC1C(CCC1=O)CC(=O)OC

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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