Catalog No.S3852

For research use only.

L-Theanine is a relaxing and nondietary amino acid found pretty much exclusively in teas from Camellia sinensis and is known to promote relaxation without sedation.

L-Theanine Chemical Structure

CAS No. 3081-61-6

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Biological Activity

Description L-Theanine is a relaxing and nondietary amino acid found pretty much exclusively in teas from Camellia sinensis and is known to promote relaxation without sedation.
In vitro

L-theanine promotes NO production in endothelial cells. L-Theanine mediated eNOS phosphorylation and NO production are PI3K/ERK pathway dependant. Treatment of EA.hy926 cells with L-theanine dose-dependently increases eNOS Ser1177 phosporylation. This increase occurrs at 2-5 min of L-theanine treatment and coincides with NO production. L-theanine confers neuroprotective effects by increasing the expression levels of PLC-beta1 and gamma1, and thereby minimizes apoptosis in the neural cells[2].

In vivo L-theanine can enhance innate immune function by regulating the secretion of immune cytokines. Its administration increases the splenic organ index and decreases the contents of ILs-4/6/10 and the ratio of IL-4/IFN-γ in the serum. High-dose L-theanine administration increases the levels of dopamine and 5-hydroxytryptamine in the pituitary and hippocampus, resulting in decrease in corticosterone level in the serum. L-theanine administration decreases the mRNA expressions of PLC isomers in the liver and PLC-γ1 and PLC-δ1 in the spleen. mRNA expressions of PLC-βf1 in the spleen and PLC isomers mRNA in the heart are up-regulated by L-theanine administration. After being absorbed into the body, L-theanine can penetrate the blood-brain barrier and regulate the secretion of neurotransmitters of central nervous system cells and levels of hormones such as corticosterone (CORT) and adrenaline (EPI) in the serum. L-theanine can increase levels of 5-hydroxytryptamine (5-HT) and noradrenaline, and decrease levels of adrenocorticotropic hormone and CORT in the hippocampus and prefrontal cortex of rats[1]. L-theanine has been implicated in reduction of blood pressure in hypertensive rats[2]. L-theanine could inhibit the glucose uptake by downregulating the related gene expression in the small intestine of rats. Intestinal gene expression of transporters responding to amino acids absorption was stimulated by L-theanine administration[3].

Protocol (from reference)

Cell Research:[2]
  • Cell lines: EA.hy926 cells
  • Concentrations: 0.01, 0.1, 1, 10 μM
  • Incubation Time: 30 min
  • Method: EA.hy926 cells are treated with varying concentration of L-theanine (0.01 μM, 0.1 μM, 1.0 μM, 10 μM) for 30 min and after 24 h of incubation cell viability was determined using CMFDA as described elsewhere.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: Sprague-Dawley rats
  • Dosages: 0, 50, 200, and 400 mg/kg BW
  • Administration: intragastric infusion
  • (Only for Reference)

Solubility (25°C)

In vitro

Water 34 mg/mL
(195.17 mM)
DMSO Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 174.20


CAS No. 3081-61-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCNC(=O)CCC(C(=O)O)N

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04391452 Not yet recruiting Dietary Supplement: Stress Resist® (oral dietary supplement) Stress Psychological University Hospital Clermont-Ferrand June 2020 Not Applicable

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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