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L-Theanine

Cat.No.S3852

L-Theanine is a relaxing and nondietary amino acid found pretty much exclusively in teas from Camellia sinensis and is known to promote relaxation without sedation.
L-Theanine Chemical Structure

Chemical Structure

Molecular Weight: 174.2

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Quality Control

Batch: S385201 Water]34 mg/mL]false]DMSO]Insoluble]false]]]false Purity: 99.57%
99.57

Solubility

In vitro
Batch:

Water : 34 mg/mL

DMSO : Insoluble
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Chemical Information, Storage & Stability

Molecular Weight 174.2 Formula

C7H14N2O3

 Storage (From the date of receipt)
CAS No. 3081-61-6 -- Storage of Stock Solutions

Synonyms N/A Smiles CCNC(=O)CCC(C(=O)O)N

Mechanism of Action

In vitro
L-theanine promotes NO production in endothelial cells. L-Theanine mediated eNOS phosphorylation and NO production are PI3K/ERK pathway dependant. Treatment of EA.hy926 cells with L-theanine dose-dependently increases eNOS Ser1177 phosporylation. This increase occurrs at 2-5 min of L-theanine treatment and coincides with NO production. L-theanine confers neuroprotective effects by increasing the expression levels of PLC-beta1 and gamma1, and thereby minimizes apoptosis in the neural cells.
In vivo
L-theanine can enhance innate immune function by regulating the secretion of immune cytokines. Its administration increases the splenic organ index and decreases the contents of ILs-4/6/10 and the ratio of IL-4/IFN-γ in the serum. High-dose L-theanine administration increases the levels of dopamine and 5-hydroxytryptamine in the pituitary and hippocampus, resulting in decrease in corticosterone level in the serum. L-theanine administration decreases the mRNA expressions of PLC isomers in the liver and PLC-γ1 and PLC-δ1 in the spleen. mRNA expressions of PLC-βf1 in the spleen and PLC isomers mRNA in the heart are up-regulated by L-theanine administration. After being absorbed into the body, L-theanine can penetrate the blood-brain barrier and regulate the secretion of neurotransmitters of central nervous system cells and levels of hormones such as corticosterone (CORT) and adrenaline (EPI) in the serum. L-theanine can increase levels of 5-hydroxytryptamine (5-HT) and noradrenaline, and decrease levels of adrenocorticotropic hormone and CORT in the hippocampus and prefrontal cortex of rats. L-theanine has been implicated in reduction of blood pressure in hypertensive rats. L-theanine could inhibit the glucose uptake by downregulating the related gene expression in the small intestine of rats. Intestinal gene expression of transporters responding to amino acids absorption was stimulated by L-theanine administration.
References

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05808595 Completed
Stress
Ethical Naturals Inc.|KGK Science Inc.
 April 4 2023 Phase 2
NCT04391452 Completed
Stress Psychological
University Hospital Clermont-Ferrand
 July 1 2020 Not Applicable

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