Ipriflavone (Osteofix)

Catalog No.S2422

Ipriflavone (Osteofix) Chemical Structure

Molecular Weight(MW): 280.32

Ipriflavone (7-Isopropoxyisoflavon) is used to inhibit bone resorption.

Size Price Stock Quantity  
USD 70 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Purity & Quality Control

Biological Activity

Description Ipriflavone (7-Isopropoxyisoflavon) is used to inhibit bone resorption.
Targets
advanced glycation end products (AGE) [2]
In vitro

Ipriflavone regulates the differentiation and biosynthetic properties of human bone marrow stromal osteoprogenitor cells (BMC) by enhancing the expression of some important matrix proteins and facilitating the mineralization process. [1] Ipriflavone treatment at 10 μM significantly inhibits advanced glycation end products (AGE) enhanced bone resorption in cultured mouse unfractionated bone cells. [2] In addition, Ipriflavone treatment potently enhances calcium uptake by duodenal cells. [3] Ipriflavone inhibits the proliferation and DNA synthesis of MDA-231 cells in a dose-dependent manner, and blocks the ligand-induced phosphorylation of Tyr845 of the EGFR. Ipriflavone treatment at 50 μM does not induce apoptosis of MDA-231 cells. [4]

In vivo Oral administration of Ipriflavone at 12 mg but not 6 mg significantly inhibits the development of new osteolytic bone metastases of MDA-231 cells, suppresses the progression of established osteolytic lesions, and reduces the number of osteoclasts adjacent to cancer cells in bone metastases. Consistent with the in vitro activity, Ipriflavone treatment at 6 or 12 mg/day significantly inhibits tumor growth of MDA-231 xenografts with 25% and 45% reductions by volume and 47% and 63% by weight, respectively, compared to untreated mice, and prolongs the life of tumor-bearing mice. [4] LD50: Mice >2.5g/kg (i.p.), >5g/kg (s.c.), >10g/kg (i.g.); Rats >2.5g/kg (i.p.), >5g/kg (s.c.), >10g/kg (i.g.) [5]

Protocol

Cell Research:[4]
- Collapse
  • Cell lines: MDA-231 cells
  • Concentrations: Dissolved in absolute ethanol, final concentrations ~50 μM
  • Incubation Time: 24, 48, 72 and 96 hours
  • Method: Cells are exposed to various concentrations of Ipriflavone for 24, 48, 72 and 96 hours. Cells are treated with trypan blue to estimate the number of viable cells. For thymidine incorporation analysis, [3H]Thymidine (1 μCi) is added, and cells are harvested onto glass fiber filters 4 hours later. Radioactivity is determined by scintillation counting.
    (Only for Reference)
Animal Research:[4]
- Collapse
  • Animal Models: Male athymic nude mice (ICR-nu/nu) injected s.c. or intracardially with MDA-231 human breast cancer cells
  • Formulation: Suspended in water containing 0.5% (w/v) methylcellulose
  • Dosages: ~12 mg/day
  • Administration: Oral administration
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 56 mg/mL (199.77 mM)
Ethanol 2 mg/mL (7.13 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 280.32
Formula

C18H16O3

CAS No. 35212-22-7
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Related Others Products

Tags: buy Ipriflavone (Osteofix) | Ipriflavone (Osteofix) supplier | purchase Ipriflavone (Osteofix) | Ipriflavone (Osteofix) cost | Ipriflavone (Osteofix) manufacturer | order Ipriflavone (Osteofix) | Ipriflavone (Osteofix) distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID