Ipriflavone (Osteofix)

Catalog No.S2422

For research use only.

Ipriflavone (7-Isopropoxyisoflavon) is used to inhibit bone resorption.

Ipriflavone (Osteofix) Chemical Structure

CAS No. 35212-22-7

Purity & Quality Control

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Biological Activity

Description Ipriflavone (7-Isopropoxyisoflavon) is used to inhibit bone resorption.
Targets
advanced glycation end products (AGE) [2]
In vitro

Ipriflavone regulates the differentiation and biosynthetic properties of human bone marrow stromal osteoprogenitor cells (BMC) by enhancing the expression of some important matrix proteins and facilitating the mineralization process. [1] Ipriflavone treatment at 10 μM significantly inhibits advanced glycation end products (AGE) enhanced bone resorption in cultured mouse unfractionated bone cells. [2] In addition, Ipriflavone treatment potently enhances calcium uptake by duodenal cells. [3] Ipriflavone inhibits the proliferation and DNA synthesis of MDA-231 cells in a dose-dependent manner, and blocks the ligand-induced phosphorylation of Tyr845 of the EGFR. Ipriflavone treatment at 50 μM does not induce apoptosis of MDA-231 cells. [4]

In vivo Oral administration of Ipriflavone at 12 mg but not 6 mg significantly inhibits the development of new osteolytic bone metastases of MDA-231 cells, suppresses the progression of established osteolytic lesions, and reduces the number of osteoclasts adjacent to cancer cells in bone metastases. Consistent with the in vitro activity, Ipriflavone treatment at 6 or 12 mg/day significantly inhibits tumor growth of MDA-231 xenografts with 25% and 45% reductions by volume and 47% and 63% by weight, respectively, compared to untreated mice, and prolongs the life of tumor-bearing mice. [4] LD50: Mice >2.5g/kg (i.p.), >5g/kg (s.c.), >10g/kg (i.g.); Rats >2.5g/kg (i.p.), >5g/kg (s.c.), >10g/kg (i.g.) [5]

Protocol (from reference)

Cell Research:[4]
  • Cell lines: MDA-231 cells
  • Concentrations: Dissolved in absolute ethanol, final concentrations ~50 μM
  • Incubation Time: 24, 48, 72 and 96 hours
  • Method: Cells are exposed to various concentrations of Ipriflavone for 24, 48, 72 and 96 hours. Cells are treated with trypan blue to estimate the number of viable cells. For thymidine incorporation analysis, [3H]Thymidine (1 μCi) is added, and cells are harvested onto glass fiber filters 4 hours later. Radioactivity is determined by scintillation counting.
  • (Only for Reference)
Animal Research:[4]
  • Animal Models: Male athymic nude mice (ICR-nu/nu) injected s.c. or intracardially with MDA-231 human breast cancer cells
  • Dosages: ~12 mg/day
  • Administration: Oral administration
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 56 mg/mL
(199.77 mM)
Ethanol 2 mg/mL
(7.13 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 280.32
Formula

C18H16O3

CAS No. 35212-22-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(C)OC1=CC2=C(C=C1)C(=O)C(=CO2)C3=CC=CC=C3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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