Hydroxytyrosol

Catalog No.S3826 Batch:S382601

Technical Data

Formula	C₈H₁₀O₃
Molecular Weight	154.16	CAS No.	10597-60-1
Solubility (25°C)*	In vitro	DMSO	30 mg/mL (194.6 mM)


* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Hydroxytyrosol (3,4-dihydroxyphenylethanol) is one of the main phenolic components of olive oil with excellent antioxidant, antimicrobial and anticarcinogenic activities.
In vitro	Hydroxytyrosol decreases the intracellular reactive oxygen species (ROS) level in MCF10A cells but not in MCF7 or MDA-MB-231 cells. Hydroxytyrosol prevents oxidative DNA damage in the three breast cell lines: MCF10A, MDA-MB-231 and MCF7 cells. It is a potent antioxidant because of its marked antioxidant activity, its ability to scavenge oxygen and nitrogen free radicals, to inhibit Low Density Lipoprotein (LDL) oxidation, platelet aggregation and endothelial cell activation and its protection against DNA damage. Hydroxytyrosol is able to reduce the synthesis of prostaglandin E2 blocking the transcription of COX-2 and 5-lipooxygenase, thereby reducing the chronic influence associated with diseases such as cancer^[1].
In vivo	HT administration protects against hepatic I/R injury, as indicated by the decreased levels of serum aminotransferase and less parenchymal necrosis and apoptosis. Serum levels of tumor necrosis factor-α, interleukin-6, macrophage inflammatory protein 2, as well as reactive oxygen species (ROS) content in liver tissues, are all decreased by HT^[2].

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines MDA-MB-231, MCF10A and MCF7 cells Concentrations 1 to 100 μM Incubation Time 24 hours Method Cells are seeded at 2 × 10³ cells/well (MCF7) or 1 × 10³ cells/well (MDA-MB-231 and MCF10A) into 96-well culture plates (flat bottom) (100 μL of cell suspension/well). At 24 h after plating, 100 μL of fresh culture medium, with different concentrations of HT is added in triplicate to the wells. Plates are incubated for 24 h or 24 h followed by a 48 h proliferation period with fresh medium at 37 °C and 5% CO2. At these time points, medium is removed and 200 μL of fresh RPMI medium without phenol red that contained XTT (200 μg/mL) and PMS (20 μg/mL) is added. Plates are incubated for 3 h at 37 °C in 5% CO2 and absorbance is measured at 450 nm wavelength (620 nm as reference) in a plate reader.
Animal Study:^{^[2]}	Animal Models A mouse model of partial hepatic I/R injury (Male BALB/c mice) Dosages 1 or 10 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
MDA-MB-231, MCF10A and MCF7 cells
Concentrations
1 to 100 μM
Incubation Time
24 hours
Method
Cells are seeded at 2 × 10³ cells/well (MCF7) or 1 × 10³ cells/well (MDA-MB-231 and MCF10A) into 96-well culture plates (flat bottom) (100 μL of cell suspension/well). At 24 h after plating, 100 μL of fresh culture medium, with different concentrations of HT is added in triplicate to the wells. Plates are incubated for 24 h or 24 h followed by a 48 h proliferation period with fresh medium at 37 °C and 5% CO2. At these time points, medium is removed and 200 μL of fresh RPMI medium without phenol red that contained XTT (200 μg/mL) and PMS (20 μg/mL) is added. Plates are incubated for 3 h at 37 °C in 5% CO2 and absorbance is measured at 450 nm wavelength (620 nm as reference) in a plate reader.

Animal Study:^{^[2]}

Animal Models
A mouse model of partial hepatic I/R injury (Male BALB/c mice)
Dosages
1 or 10 mg/kg
Administration
i.p.

References

Selleck's Hydroxytyrosol has been cited by 2 publications

Hydroxytyrosol Recovers SARS-CoV-2-PLpro-Dependent Impairment of Interferon Related Genes in Polarized Human Airway, Intestinal and Liver Epithelial Cells [ Antioxidants (Basel), 2022, 11(8)1466]	PubMed: 36009185
Combination Treatment with Hydroxytyrosol and Vitamin E Improves NAFLD-Related Fibrosis [ Nutrients, 2022, 14(18)3791]	PubMed: 36145170

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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