Catalog No.S4047

For research use only.

Bisacodyl (INN) is a stimulant laxative drug that works directly on the colon to produce a bowel movement.

Bisacodyl Chemical Structure

CAS No. 603-50-9

Purity & Quality Control

Related Other Products

Biological Activity

Description Bisacodyl (INN) is a stimulant laxative drug that works directly on the colon to produce a bowel movement.
In vitro

Bisacodyl (10 μg/mL) increases mRNA expression level of TNF-α, COX2 and PGE2 in Raw264.7 cells. [1]

In vivo Bisacodyl (20 mg/kg) results in a decrease in AQP3 protein expression and increased mRNA expression level of TNF-α in the colon of rats. [1] Bisacodyl inhibits water absorption in rat jejunum, ileum, and colon, the degree of inhibition is linearly related to the logarithm of the bisacodyl concentration over the range of 0.05 mg to 2.0 mg per 100 mL. [2] Bisacodyl (10 mg/kg, intragastrically) induces a significant decrease in jejunal NOS activity in rats. Bisacodyl (10 mg/kg, intragastrically) increases the distance traveled by the marker in all time periods. [3] Bisacodyl (5.9 mg/kg) decreases significantly jejunal and colonic (Na + K) ATPase activity as compared to saline-treated rats. Bisacodyl (5.9 mg/kg) increases significantly jejunal and colonic PGE2 content and stimulates jejunal and colonic adenyl cyclase activity as compared to those in control rats without affecting cAMP content. [4] Bisacodyl (4.3 mg/kg) coupled with AOM increases the number of crypt per focus, but not the number of tumors in rats. Bisacodyl (43 mg/kg) significantly increases the number of crypt per focus and tumors in rats. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 361.39


CAS No. 603-50-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=O)OC1=CC=C(C=C1)C(C2=CC=C(C=C2)OC(=O)C)C3=CC=CC=N3

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03279341 Completed Drug: polyethylene glycol|Drug: Bisacodyl|Drug: Prucalopride Chronic Constipation University Hospital Gasthuisberg December 3 2012 Phase 4
NCT01424228 Completed Drug: placebo|Drug: prucalopride Constipation Shire|Takeda April 6 2011 Phase 4
NCT01147926 Completed Drug: Placebo|Drug: Prucalopride Male Subjects With Chronic Constipation Shire|Takeda September 23 2010 Phase 3
NCT01116206 Completed Drug: Prucalopride|Drug: Placebo Constipation Johnson & Johnson Pharmaceutical Research & Development L.L.C. May 2010 Phase 3

(data from, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Bisacodyl | Bisacodyl ic50 | Bisacodyl price | Bisacodyl cost | Bisacodyl solubility dmso | Bisacodyl purchase | Bisacodyl manufacturer | Bisacodyl research buy | Bisacodyl order | Bisacodyl mouse | Bisacodyl chemical structure | Bisacodyl mw | Bisacodyl molecular weight | Bisacodyl datasheet | Bisacodyl supplier | Bisacodyl in vitro | Bisacodyl cell line | Bisacodyl concentration | Bisacodyl nmr