α-Hederin

Catalog No.S3914

For research use only.

α-hederin is a water-soluble pentacyclic triterpenoid saponin which has shown hemolytic and apoptotic properties.

α-Hederin Chemical Structure

CAS No. 27013-91-8

Purity & Quality Control

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Biological Activity

Description α-hederin is a water-soluble pentacyclic triterpenoid saponin which has shown hemolytic and apoptotic properties.
In vitro

α-hederin shows strong inhibitory activity on the growth of breast cancer cells and induces apoptosis in these cells. α-hederin induces depolarization of mitochondrial membrane potential which releases Apaf-1 and cytochrome c from the intermembrane space into the cytosol, where they promote caspase-3 and caspase-9 activation[1].

In vivo After a single i.v. (2 mg/kg) administration, the mean plasma clearance (CL), volume of distribution (VSS) and elimination half-life (t1/2) of α-hederin in rtas are 0.24 L/h/kg, 0.25 L/kg and 2.67 h, respectively. The oral bioavailability (F) of α-hederin in rats is about 0.14%, which might result from the poor intestinal absorption and/or extensive biliary excretion. α-Hederin decreases the hepatotoxicity of cadmium in mice by inducing hepatic metallothionein I/II, and the mechanism partly involved the upregulation of metal-lothionein expression mediated by TNF-α and IL-6[2].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: The human breast cancer cell lines MCF-7 and MDA-MB-231
  • Concentrations: 0.08, 0.4, 2 and 10 μg/ml
  • Incubation Time: 12, 24 and 48 h
  • Method: The MTT assay is used to measure the inhibition of growth by α-hederin in breast cancer cell lines. Briefly, 5×103 cells are seeded into a 96-well plate in triplicate and 8 h later α-hederin is added into the wells at the indicated final concentrations (0.08, 0.4, 2 and 10 μg/ml), while cells cultured in medium with 0.05% DMSO as a negative control. After incubation with α-hederin for 12, 24 and 48 h, the medium in each well is replaced with 20 μl of MTT at 5 mg/ml final concentration, and 4 h later 150 μl DMSO/well is added to dissolve the formed violet formazan crystals within metabolically viable cells. The plates are incubated at room temperature for 15 min and then read at 490 nm with a microplate reader. The percentage of growth inhibition is calculated as (OD of the control-OD of the experiment samples)/OD of the control × 100.
  • (Only for Reference)
Animal Research:[2]
  • Animal Models: Sprague-Dawley (SD) rats
  • Dosages: 10 mg/kg (p.o.) or 2 mg/kg (i.v.)
  • Administration: p.o. or i.v.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 100 mg/mL
(133.16 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 750.96
Formula

C41H66O12

CAS No. 27013-91-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1C(C(C(C(O1)OC2C(C(COC2OC3CCC4(C(C3(C)CO)CCC5(C4CC=C6C5(CCC7(C6CC(CC7)(C)C)C(=O)O)C)C)C)O)O)O)O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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