5-Aminolevulinic acid HCl

Catalog No.S2553 Synonyms: 5-ALA hydrochloride

For research use only.

5-Aminolevulinic Acid HCl(5-ALA hydrochloride) is an intermediate in the porphyrin synthesis pathway, used as a photosensitizing agent and a antineoplastic agent.

5-Aminolevulinic acid HCl Chemical Structure

CAS No. 5451-09-2

Selleck's 5-Aminolevulinic acid HCl has been cited by 8 Publications

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Biological Activity

Description 5-Aminolevulinic Acid HCl(5-ALA hydrochloride) is an intermediate in the porphyrin synthesis pathway, used as a photosensitizing agent and a antineoplastic agent.
In vitro

5-aminolevulinic acid (5-ALA)-Photodynamic therapy (PDT) results in down regulation of nuclear factor kappa B (NFkappaB) and baculovirus inhibitor-of-apoptosis repeat containing-3 (BIRC-3) protein. 5-aminolevulinic acid (5-ALA)-Photodynamic therapy (PDT) causes increase in Bax:Bcl-2 ratio and mitochondrial release of cytochrome c and apoptosis-inducing factor (AIF). [1] 5-aminolevulinic acid yields reactive oxygen species upon metal-catalyzed oxidation and causes in vivo and in vitro impairment of rat liver mitochondria and DNA damage. 5-aminolevulinic acid induces a dose-dependent damage in nuclear and mitochondrial DNA in human SVNF fibroblasts and rat PC12 cells. [2] 5-aminolevulinic acid dose-dependently decreases cAMP levels (maximal inhibition of 38%, at 1 mM), due to an inhibition of basaladenylate cyclase activity. 5-aminolevulinic acid also inhibits fluoride- and Gpp(NH)p-stimulated, but not the forskolin-stimulated adenylate cyclase activity. 5-aminolevulinic acid also inhibits the activity of adenylate cyclase in membranes isolated from rat cortex and striatum and from human cortex. [3] 5-aminolevulinic acid (0-3mM) dose-dependently inhibits glutamate uptake by astrocyte cultures. 5-aminolevulinic acid significantly reduces both the K(m) and V(max) of glutamate uptake indicating an uncompetitive inhibition. 5-aminolevulinic acid significantly increases astrocyte lipoperoxidation in astrocytes incubated under these conditions. [4] 5-aminolevulinic acid mediated sonodynamic therapy exhibits synergistic apoptotic effects on THP-1 macrophages, involving excessive intracellular reactive oxygen species generation and MMP loss. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 167.59


CAS No. 5451-09-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C(CC(=O)O)C(=O)CN.Cl

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04626661 Recruiting Device: COMET measurement system Mitochondrial Oxygenation Measurement|Measurement Error|Healthy Sanquin-LUMC J.J van Rood Center for Clinical Transfusion Research|Leiden University Medical Center June 15 2020 --
NCT00241670 Completed Drug: 5-aminolevulinic acid (5-ALA) Brain Cancer|Brain Tumors|Cancer of Brain|Primary Brain Tumors|Brain Tumor Primary medac GmbH October 1999 Phase 3

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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