Valproic Acid (NSC 93819) sodium salt

For research use only.

Catalog No.S1168 Synonyms: Sodium valproate

26 publications

Valproic Acid (NSC 93819) sodium salt Chemical Structure

CAS No. 1069-66-5

Valproic Acid sodium salt (NSC 93819, Sodium valproate) is a HDAC inhibitor by selectively inducing proteasomal degradation of HDAC2, used in the treatment of epilepsy, bipolar disorder and prevention of migraine headaches. Valproic acid induces Notch1 signaling in small cell lung cancer (SCLC) cells. Valproic acid is under investigation for treatment of HIV and various cancers. Valproic acid (VPA) induces autophagy and mitophagy by upregulation of BNIP3 and mitochondrial biogenesis by upregulating PGC-1α.

Selleck's Valproic Acid (NSC 93819) sodium salt has been cited by 26 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Valproic Acid sodium salt (NSC 93819, Sodium valproate) is a HDAC inhibitor by selectively inducing proteasomal degradation of HDAC2, used in the treatment of epilepsy, bipolar disorder and prevention of migraine headaches. Valproic acid induces Notch1 signaling in small cell lung cancer (SCLC) cells. Valproic acid is under investigation for treatment of HIV and various cancers. Valproic acid (VPA) induces autophagy and mitophagy by upregulation of BNIP3 and mitochondrial biogenesis by upregulating PGC-1α.
Targets
HDAC [1]
(Cell-free assay)
Autophagy [1]
(Cell-free assay)
GABA receptor [1]
(Cell-free assay)
In vitro

Valproic acid acts through a distinct pathway that involves direct inhibition of histone deacetylase (IC(50) for HDAC1 = 0.4 mM). Valproic acid mimics the histone deacetylase inhibitor trichostatin A, causing hyperacetylation of histones in cultured cells. Valproic acid, like trichostatin A, also activates transcription from diverse exogenous and endogenous promoters. Valproic acid and trichostatin A have remarkably similar teratogenic effects in vertebrate embryos, while non-teratogenic analogues of valproic acid do not inhibit histone deacetylase and do not activate transcription. [1] Valproic acid induces proliferation of peroxisomes in the rodent liver. Valproic acid at a concentration of 1 mM induces relief of this repression by Gal4 fusions of N‐CoR, TR or PPARδ in a cell line expressing the ligand‐binding domain of PPARδ fused to the DNA‐binding domain of the glucocorticoid receptor (GR) together with a GR‐controlled reporter gene. Valproic acid induces accumulation of hyperacetylated histone and inhibits HDAC activity. Valproic acid induces a specific type of differentiation characterized by reduced proliferation, morphological alterations, marker gene expression and particularly the accumulation of the AP-2 transcription factor as a potential marker of neuronal or neural crest cell-like differentiation in F9 teratocarcinoma cells. Valproic acid impairs cell proliferation or survival as indicated by decreased incorporation of [3H]thymidine in F9 and P19 teratocarcinoma cells. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
HEK293 NHvPRWJHfW6ldHnvckBie3OjeR?= M3;4e|EhdU1? M3vFNGlv[3KnYYPlJIlvKHC{b4TlbY4h\Gm|dXzmbYRmKGm|b33ldoF{\SCuZY\lcEBqdiCKRVuyPVMh[2WubIOgZZQhOSCvTTDifUBqdW23bn;icI91 M1mzOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NU[2O|MzLz5zN{W2Olc{OjxxYU6=
HEK293 NUTwSnRITnWwY4Tpc44h[XO|YYm= NWjIT|FvOSCvTR?= MVXJcoNz\WG|ZTDpckBIWlB5ODDwdo91\WmwIHzleoVtKGmwIFjFT|I6OyClZXzsd{BifCBzIH3NJIJ6KGmvbYXuc4Jtd3R? NV;YO2xMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1OlY4OzJpPkG3OVY3PzN{PD;hQi=>
A549 Ml33SpVv[3Srb36gZZN{[Xl? MWOxOVAhfU1? MUCyOEBpenN? M4rPS2lvcGmkaYTpc44hd2ZiaIXtZY4hUESDQzDpckBCPTR7IHPlcIx{KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWtTFQh[WOndInsZZRqd25iYYSgNVUxKHWPIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4S= NG\hfIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK5OFg1PCd-MUiyPVQ5PDR:L3G+
GM15850 M3rKemZ2dmO2aX;uJIF{e2G7 MX60NFAhfU1? MUWxNkBpenN? M{i4SmlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBIVTF3OEWwJINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKHSxdHHsJIFk\XS7bHH0[YQhcGm|dH;u[UBt\X[nbDDheEA1ODBidV2gZYZ1\XJiMUKgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> NHjzPHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
PC12 M1fnOGZ2dmO2aX;uJIF{e2G7 MUexJJVO M{exVFI1KGi{cx?= MVnJcoR2[3Srb36gc4Yh[XW2b4DoZYd6KGmwIILheEB{fGGkbHWgbY5lfWOrYnzlJHBEOTJiY3XscJMh\XiycnXzd4lv\yCDNUPUJIFteGijLYP5cpVkdGWrbjDhd5Nme3OnZDDhd{BCPTOWIHHsdIhiNXO7boXjcIVqdiClbHXhdoFv[2ViYYSgNUB2VSCjZoTldkAzPCCqcoOgZpkh\GWwc3n0c41mfHKrYzDhcoFtgXOrcx?= NIrJOpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO5NVk1QSd-MUizPVE6PDl:L3G+
PC12 MnHsSpVv[3Srb36gZZN{[Xl? NVf6UZl{OSC3TR?= MWG5OkBpenN? MYnJcoR2[3Srb36gc4Yh[XW2b4DoZYd6KGmwIILheEB{fGGkbHWgbY5lfWOrYnzlJHBEOTJiY3XscJMh\XiycnXzd4lv\yCHR1\QMWhFWTd2IHHzd4V{e2WmIHHzJJNwdHWkbHWgSWdHWC2KRGG3OEBkdGWjcnHuZ4Uh[XRiMTD1UUBi\nSncjC5OkBpenNiYomg[IVve2m2b33leJJq[yCjbnHsfZNqew>? MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7MUm0PUc,OTh|OUG5OFk9N2F-
SK-N-MC NGmweWVHfW6ldHnvckBie3OjeR?= MWSxJI1O Moe2OFghcHK| NVvIUVNtUW6mdXP0bY9vKG:oIHH1eI9xcGGpeTDpckBpfW2jbjDTT{1PNU2FIHPlcIx{KGW6cILld5NqdmdiRVfGVE1JTFF5NDDhd5Nme3OnZDDhd{Bz\WS3Y4Tpc44hcW5iRVfGVE1JTFF5NDDh[4dz\WejdHnvckBifCBzIIXNJIFnfGW{IES4JIhzeyCkeTDk[Y5{cXSxbXX0dolkKGGwYXz5d4l{ MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN7MUm0PUc,OTh|OUG5OFk9N2F-
HL60 MoC5SpVv[3Srb36gZZN{[Xl? M4LSVVEhdU1? MkfoNlQhcHK| NV7VOXRuUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEiONkCgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm|dH;u[UBJOyCjY3X0fYxifGmxbjDheEAyKG2PIHHmeIVzKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4T0bY5oKG2ndHjv[C=> NYLKZpBuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkWzNFQ5QTZpPkK1N|A1QDl4PD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Acetyl Histone H3 ; 

PubMed: 28542253     


Histone acetylation levels were determined by western blotting after 72 hours of sodium valproate treatment and was used as an indicator of HDAC inhibition.

Active caspase-3 / PARP / Cleaved PARP ; 

PubMed: 28542253     


To confirm the induction of apoptosis western blotting was carried out on all three cell lines treated with ≤5 mM valproate for 72 hours.

acetyl-H4; 

PubMed: 20025549     


Changes in histone acetylation after valproic acid (VPA) exposure. LS174T and HCT116 cells were exposed to varying concentrations of VPA for 16 hours. Cellular protein extracts were prepared, as described in Materials and Methods, and analyzed by immunoblot assay with antibody against acetylated histone H4 (acetyl-H4). β-actin was included as a control to show equivalent protein loading.

28542253 20025549
Growth inhibition assay
Cell viability; 

PubMed: 28498322     


The viability of MIHA, HepG2, and SNU475 cells was determined by EZ-Cytox assay after 48-h exposure to the indicated concentration of VPA; 

28498322
In vivo Valproic acid delays growth of the primary tumors in the MT‐450 rat breast cancer model. [2]

Protocol

Solubility (25°C)

In vitro DMSO 33 mg/mL (198.56 mM)
Water 33 mg/mL (198.56 mM)
Ethanol ''33 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 166.19
Formula

C8H15NaO2

CAS No. 1069-66-5
Storage powder
in solvent
Synonyms Sodium valproate
Smiles CCCC(CCC)C(=O)[O-].[Na+]

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03919292 Recruiting Drug: Neratinib|Drug: Divalproex Sodium Solid Tumor Adult Virginia Commonwealth University|Puma Biotechnology Inc. May 1 2019 Phase 1|Phase 2
NCT03681158 Completed Drug: sodium valproate Epilepsy Sanofi October 5 2018 Phase 1
NCT03112889 Completed Drug: Sodium Valproate Glycogen Storage Disease Type V|McArdle Disease University College London January 2015 Phase 2
NCT00139074 Terminated Drug: Quetiapine fumarate|Drug: sodium valproate Bipolar Disorder AstraZeneca July 2005 Phase 4

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

HDAC Signaling Pathway Map

Related HDAC Products

Tags: buy Valproic Acid (NSC 93819) sodium salt | Valproic Acid (NSC 93819) sodium salt supplier | purchase Valproic Acid (NSC 93819) sodium salt | Valproic Acid (NSC 93819) sodium salt cost | Valproic Acid (NSC 93819) sodium salt manufacturer | order Valproic Acid (NSC 93819) sodium salt | Valproic Acid (NSC 93819) sodium salt distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID