Tacedinaline (CI994)

Catalog No.S2818 Synonyms: PD-123654, GOE-5549, Acetyldinaline

Tacedinaline (CI994) Chemical Structure

Molecular Weight(MW): 269.3

Tacedinaline (CI994) is a selective class I HDAC inhibitor with IC50 of 0.9, 0.9, 1.2, and >20 μM for human HDAC 1, 2, 3, and 8, respectively. Phase 3.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 70 In stock
USD 270 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

2 Customer Reviews

  • A, 5×106 HeLa, A549, 293T, and H1299 cells were seeded in 10-cm cell culture dishes on day 0 and treated with dimethyl sulfoxide, 10 μM CI994, or 1 μM RGFP966 for 12 h. Cell lysates were collected for Western blotting analysis of Apaf-1 and -actin.

    J Biol Chem, 2016, 291(14):7386-95. Tacedinaline (CI994) purchased from Selleck.

    Apoptosis marker analysis. Daoy and D283 Med cells were exposed to various HDACi 24 h before total RNA samples were prepared for qRT-PCR. Daoy cells were exposed to DMSO, 25 nM TSA, 1 μM SAHA, 1.5 μM MS-275, 0.7 μM mocetinostat, 7 μM tacedinaline, 1.5 nM romidepsin and 2 μM parthenolide, respectively. D283 Med cells were exposed to DMSO, 40 nM TSA, 1.5 μM SAHA, 2 μM MS-275, 0.3 μM mocetinostat, 8 μM tacedinaline, 1 nM romidepsin and 4 μM parthenolide, respectively. Expression of the apoptosis markers BAX and BCL2, as well as the apoptotic BAX/BCL2 ratios, are shown. * p < 0.05; **p < 0.01.

    Cell Oncol (Dordr), 2017, 40(3):263-279. Tacedinaline (CI994) purchased from Selleck.

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Tacedinaline (CI994) is a selective class I HDAC inhibitor with IC50 of 0.9, 0.9, 1.2, and >20 μM for human HDAC 1, 2, 3, and 8, respectively. Phase 3.
HDAC1 [1]
(Cell-free assay)
HDAC2 [1]
(Cell-free assay)
HDAC3 [1]
(Cell-free assay)
0.9 μM 0.9 μM 1.2 μM
In vitro

CI-994 (< 160 mM) shows cytostatic effect with concomitant increase at G0/G1 phase, a reduction at S phase level and increased apoptosis in A-549 and LX-1 cells. [2] CI-994 inhibits growth of LNCaP cell with IC50 of 7.4 μM. [3] CI-994 exerts activity against several tumor cell lines with greater cytotoxicity against the solid tumors relative to both the leukemia and normal fibroblast cell lines. [4] CI-994 inhibits growth of rat leukemia BCLO cells with IC50 of 2.5 μM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-231 cells MYrHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>? MmTwOUBl[Xm| MoruS5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDh[pRmeiB3IHThfZMh[nliTWTUJIF{e2G7LDDHTVUxRTBwMUeg{txO M3Tke|E5PTR|OUCy
human PC3 cells NGnMVlBIem:5dHigbY5pcWKrdHnvckBie3OjeR?= M1PUSlUh\GG7cx?= NGHje4dIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBRSzNiY3XscJMh[W[2ZYKgOUBl[Xm|IHL5JG1VXCCjc4PhfUwhT0l3ME2wMlI6KM7:TR?= NVTCcGF[OTh3NEO5NFI>
human HCT116 cells M{HUS2N6fG:2b4jpZ:Kh[XO|YYm= MUjDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxRTRizszN Mm\XNVc6PDF4MkW=
293T cells MkHUSpVv[3Srb36gZZN{[Xl? NFv6[|NKdmirYnn0bY9vKG:oIFjERWMhcW5iMkmzWEBk\WyuczygTWM2OD14IN88US=> M17yOVE4QTRzNkK1
human LNCap M3rBbnBzd2yrZnXyZZRqd25iYYPzZZk> NXroeFBiSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDMUmNieCCkeTDNWHQh[XO|YYpvwKwhUUN3ME23MlQh|ryP MYOxPFE3PjR4NR?=
human NCI-H661 cells MXTQdo9tcW[ncnH0bY9vKGG|c3H5 M3j4SFQ5KGh? MmLZRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCQQ1mtTFY3OSClZXzsd{Bi\nSncjC0PEBpenNuIFnDOVA:OjBizszN MXKxPFY6OjN7Nx?=
human NCI-H661 cells NU\BSppyTnWwY4Tpc44h[XO|YYm= MYq2JIg> M2rueWlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBPS0lvSE[2NUBk\WyuczDhd5Nme3OnZDDhd{BpcXO2b37lJGg1KGGlZYT5cIF1cW:wIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= NVPHPJp7OTh4OUKzPVc>

... Click to View More Cell Line Experimental Data

In vivo CI-994 exerts demonstrated antitumor activity against several tumor models, including the chemo-resistant mouse pancreatic ductal carcinoma as well as the human prostate tumor model LNCaP. [4]


Cell Research:


+ Expand
  • Cell lines: LNCaP cell lines
  • Concentrations: 2.5 μM
  • Incubation Time: 2-4 days
  • Method:

    LNCaP cell lines are maintained in RPMI 1640 medium containing 10% fetal bovine serum, 1% penicillin and streptomycin, as the complete culture medium. Cells (2×104) are seeded in 24-well plates and incubated in a 5% CO2 incubator at 37 °C for 1 day. Cultures are treated with CI-994, alone and in combination on day 2 and 4. Cells are washed on day 2 and 4 and media are changed. Mitochondrial metabolism is measured as a marker for cell growth by adding 100 μL/well MTT (5 mg/mL in medium) with 2 hours incubation at 37 °C on Day 6. Crystals formed are dissolved in 500 μL of DMSO. The absorbance is determined using a microplate reader at 560 nm. The absorbance data are converted into cell proliferation percentage. Each assay is performed in triplicate.

    (Only for Reference)
Animal Research:


+ Expand
  • Animal Models: human prostate tumor model LNCaP
  • Formulation: 5% ethanol, 1% P.O.E. and 94% dH20
  • Dosages: 535 mg/kg
  • Administration: Administered via oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 54 mg/mL (200.51 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 269.3


CAS No. 112522-64-2
Storage powder
in solvent
Synonyms PD-123654, GOE-5549, Acetyldinaline

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT00005093 Completed Lung Cancer Pfizer December 1999 Phase 3
NCT00004861 Completed Pancreatic Cancer Pfizer October 1999 Phase 2
NCT00005624 Completed Multiple Myeloma H. Lee Moffitt Cancer Center and Research Institute|National Cancer Institute (NCI)|Parke-Davis August 1997 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

HDAC Signaling Pathway Map

Related HDAC Products0

Tags: buy Tacedinaline (CI994) | Tacedinaline (CI994) supplier | purchase Tacedinaline (CI994) | Tacedinaline (CI994) cost | Tacedinaline (CI994) manufacturer | order Tacedinaline (CI994) | Tacedinaline (CI994) distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID