Tacedinaline (CI994)

Catalog No.S2818 Synonyms: PD-123654, GOE-5549, Acetyldinaline

Tacedinaline (CI994) Chemical Structure

Molecular Weight(MW): 269.3

Tacedinaline (CI994) is a selective class I HDAC inhibitor with IC50 of 0.9, 0.9, 1.2, and >20 μM for human HDAC 1, 2, 3, and 8, respectively. Phase 3.

Size Price Stock Quantity  
In DMSO USD 90 In stock
USD 70 In stock
USD 270 In stock
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Cited by 18 Publications

4 Customer Reviews

  • A, 5×106 HeLa, A549, 293T, and H1299 cells were seeded in 10-cm cell culture dishes on day 0 and treated with dimethyl sulfoxide, 10 μM CI994, or 1 μM RGFP966 for 12 h. Cell lysates were collected for Western blotting analysis of Apaf-1 and -actin.

    J Biol Chem, 2016, 291(14):7386-95. Tacedinaline (CI994) purchased from Selleck.

  • Apoptosis marker analysis. Daoy and D283 Med cells were exposed to various HDACi 24 h before total RNA samples were prepared for qRT-PCR. Daoy cells were exposed to DMSO, 25 nM TSA, 1 μM SAHA, 1.5 μM MS-275, 0.7 μM mocetinostat, 7 μM tacedinaline, 1.5 nM romidepsin and 2 μM parthenolide, respectively. D283 Med cells were exposed to DMSO, 40 nM TSA, 1.5 μM SAHA, 2 μM MS-275, 0.3 μM mocetinostat, 8 μM tacedinaline, 1 nM romidepsin and 4 μM parthenolide, respectively. Expression of the apoptosis markers BAX and BCL2, as well as the apoptotic BAX/BCL2 ratios, are shown. * p < 0.05; **p < 0.01.

    Cell Oncol (Dordr), 2017, 40(3):263-279. Tacedinaline (CI994) purchased from Selleck.

  • (B, C) Wild-type cells (white bars) and Ep300−/− cells (black bars) were incubated in 5 (5G) or 25 mM glucose (25G), in thepresence or absence of HDAC inhibitor CI994 (25 M) for 24 h. Cells were thenfixed and sonicated. DNA was precipitated using antibodies binding to H3K9ac(B) or H4ac (C). Quantitative PCR was run using primers flanking the promoter region and results are presented as fold change over 5G.The values represent the mean ± SEM of four to five independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. respective 5G (wild-type or knock out) or as indi-cated;†p < 0.05,††p < 0.01 vs. 25G.

    Int J Biochem Cell Biol, 2016, 81(Pt A):82-91. Tacedinaline (CI994) purchased from Selleck.

  • HCT116 cells pre-treated with HDAC inhibitor CI994 were additionally incubated in the presence or absence of 5FU (20 mg/ml) for 24 h. The cells were subjected to western blotting with antibodies to poly (ADP-ribose) polymerase (PARP) and cleaved caspase-3.

    Anticancer Res, 2018, 38(1):271-277. Tacedinaline (CI994) purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Tacedinaline (CI994) is a selective class I HDAC inhibitor with IC50 of 0.9, 0.9, 1.2, and >20 μM for human HDAC 1, 2, 3, and 8, respectively. Phase 3.
HDAC1 [1]
(Cell-free assay)
HDAC2 [1]
(Cell-free assay)
HDAC3 [1]
(Cell-free assay)
0.9 μM 0.9 μM 1.2 μM
In vitro

CI-994 (< 160 mM) shows cytostatic effect with concomitant increase at G0/G1 phase, a reduction at S phase level and increased apoptosis in A-549 and LX-1 cells. [2] CI-994 inhibits growth of LNCaP cell with IC50 of 7.4 μM. [3] CI-994 exerts activity against several tumor cell lines with greater cytotoxicity against the solid tumors relative to both the leukemia and normal fibroblast cell lines. [4] CI-994 inhibits growth of rat leukemia BCLO cells with IC50 of 2.5 μM. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human MDA-MB-231 cells MoXIS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl? M2rFTVUh\GG7cx?= M4nkVWdzd3e2aDDpcohq[mm2aX;uJI9nKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IF3UWEBie3OjeTygS2k2OD1yLkG3JO69VQ>? NUPwTpFvOTh3NEO5NFI>
human PC3 cells M3Xrb2dzd3e2aDDpcohq[mm2aX;uJIF{e2G7 MYi1JIRigXN? MXnHdo94fGhiaX7obYJqfGmxbjDv[kBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiNTDkZZl{KGK7IF3UWEBie3OjeTygS2k2OD1yLkK5JO69VQ>? NHi3bIIyQDV2M{mwNi=>
human HCT116 cells M{fwNmN6fG:2b4jpZ:Kh[XO|YYm= MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[nliTWTUJIF{e2G7LDDJR|UxRTRizszN NWfHS3NnOTd7NEG2NlU>
293T cells MmLLSpVv[3Srb36gZZN{[Xl? M4DSU2lvcGmkaYTpc44hd2ZiSFTBR{BqdiB{OUPUJINmdGy|LDDJR|UxRTZizszN MX2xO|k1OTZ{NR?=
human LNCap NXHhVopTWHKxbHnm[ZJifGmxbjDhd5NigQ>? MljPRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCOTlPhdEBjgSCPVGSgZZN{[XoxvJygTWM2OD15LkSg{txO MVuxPFE3PjR4NR?=
human NCI-H661 cells MnnsVJJwdGmoZYLheIlwdiCjc4PhfS=> NG[xdGo1QCCq MnTBRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCQQ1mtTFY3OSClZXzsd{Bi\nSncjC0PEBpenNuIFnDOVA:OjBizszN NEnMS3AyQDZ7MkO5Oy=>
human NCI-H661 cells NHXZPFRHfW6ldHnvckBie3OjeR?= MWm2JIg> M{LJd2lvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBPS0lvSE[2NUBk\WyuczDhd5Nme3OnZDDhd{BpcXO2b37lJGg1KGGlZYT5cIF1cW:wIHHmeIVzKDZiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MmDLNVg3QTJ|OUe=

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Growth inhibition assay
Cell viability; 

PubMed: 30108928     

Dose-dependent antiproliferative activities of chlordinaline against six cancer cell lines. The values represent the means of three experiments. (A) Treatment in A549 cells. (B) Treatment in A375 cells. (C) Treatment in SMMC7721 cells. (D) Treatment in HepG2 cells. (E) Treatment in H1299 cells. (F) Treatment in H460 cells.

In vivo CI-994 exerts demonstrated antitumor activity against several tumor models, including the chemo-resistant mouse pancreatic ductal carcinoma as well as the human prostate tumor model LNCaP. [4]


Cell Research:


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  • Cell lines: LNCaP cell lines
  • Concentrations: 2.5 μM
  • Incubation Time: 2-4 days
  • Method:

    LNCaP cell lines are maintained in RPMI 1640 medium containing 10% fetal bovine serum, 1% penicillin and streptomycin, as the complete culture medium. Cells (2×104) are seeded in 24-well plates and incubated in a 5% CO2 incubator at 37 °C for 1 day. Cultures are treated with CI-994, alone and in combination on day 2 and 4. Cells are washed on day 2 and 4 and media are changed. Mitochondrial metabolism is measured as a marker for cell growth by adding 100 μL/well MTT (5 mg/mL in medium) with 2 hours incubation at 37 °C on Day 6. Crystals formed are dissolved in 500 μL of DMSO. The absorbance is determined using a microplate reader at 560 nm. The absorbance data are converted into cell proliferation percentage. Each assay is performed in triplicate.

    (Only for Reference)
Animal Research:


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  • Animal Models: human prostate tumor model LNCaP
  • Formulation: 5% ethanol, 1% P.O.E. and 94% dH20
  • Dosages: 535 mg/kg
  • Administration: Administered via oral gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 54 mg/mL (200.51 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 269.3


CAS No. 112522-64-2
Storage powder
in solvent
Synonyms PD-123654, GOE-5549, Acetyldinaline

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID