Catalog No.S2818 Synonyms: PD-123654, GOE-5549, Acetyldinaline
Molecular Weight(MW): 269.3
Tacedinaline (CI994) is a selective class I HDAC inhibitor with IC50 of 0.9, 0.9, 1.2, and >20 μM for human HDAC 1, 2, 3, and 8, respectively. Phase 3.
Cited by 11 Publications
4 Customer Reviews
A, 5×106 HeLa, A549, 293T, and H1299 cells were seeded in 10-cm cell culture dishes on day 0 and treated with dimethyl sulfoxide, 10 μM CI994, or 1 μM RGFP966 for 12 h. Cell lysates were collected for Western blotting analysis of Apaf-1 and -actin.
J Biol Chem, 2016, 291(14):7386-95. Tacedinaline (CI994) purchased from Selleck.
Apoptosis marker analysis. Daoy and D283 Med cells were exposed to various HDACi 24 h before total RNA samples were prepared for qRT-PCR. Daoy cells were exposed to DMSO, 25 nM TSA, 1 μM SAHA, 1.5 μM MS-275, 0.7 μM mocetinostat, 7 μM tacedinaline, 1.5 nM romidepsin and 2 μM parthenolide, respectively. D283 Med cells were exposed to DMSO, 40 nM TSA, 1.5 μM SAHA, 2 μM MS-275, 0.3 μM mocetinostat, 8 μM tacedinaline, 1 nM romidepsin and 4 μM parthenolide, respectively. Expression of the apoptosis markers BAX and BCL2, as well as the apoptotic BAX/BCL2 ratios, are shown. * p < 0.05; **p < 0.01.
Cell Oncol (Dordr), 2017, 40(3):263-279. Tacedinaline (CI994) purchased from Selleck.
(B, C) Wild-type cells (white bars) and Ep300−/− cells (black bars) were incubated in 5 (5G) or 25 mM glucose (25G), in thepresence or absence of HDAC inhibitor CI994 (25 M) for 24 h. Cells were thenfixed and sonicated. DNA was precipitated using antibodies binding to H3K9ac(B) or H4ac (C). Quantitative PCR was run using primers flanking the promoter region and results are presented as fold change over 5G.The values represent the mean ± SEM of four to five independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. respective 5G (wild-type or knock out) or as indi-cated;†p < 0.05,††p < 0.01 vs. 25G.
Int J Biochem Cell Biol, 2016, 81(Pt A):82-91. Tacedinaline (CI994) purchased from Selleck.
HCT116 cells pre-treated with HDAC inhibitor CI994 were additionally incubated in the presence or absence of 5FU (20 mg/ml) for 24 h. The cells were subjected to western blotting with antibodies to poly (ADP-ribose) polymerase (PARP) and cleaved caspase-3.
Anticancer Res, 2018, 38(1):271-277. Tacedinaline (CI994) purchased from Selleck.
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|Description||Tacedinaline (CI994) is a selective class I HDAC inhibitor with IC50 of 0.9, 0.9, 1.2, and >20 μM for human HDAC 1, 2, 3, and 8, respectively. Phase 3.|
CI-994 (< 160 mM) shows cytostatic effect with concomitant increase at G0/G1 phase, a reduction at S phase level and increased apoptosis in A-549 and LX-1 cells.  CI-994 inhibits growth of LNCaP cell with IC50 of 7.4 μM.  CI-994 exerts activity against several tumor cell lines with greater cytotoxicity against the solid tumors relative to both the leukemia and normal fibroblast cell lines.  CI-994 inhibits growth of rat leukemia BCLO cells with IC50 of 2.5 μM. 
|In vivo||CI-994 exerts demonstrated antitumor activity against several tumor models, including the chemo-resistant mouse pancreatic ductal carcinoma as well as the human prostate tumor model LNCaP. |
-  Moradei OM, et al. J Med Chem. 2007, 50(23), 5543-5546.
-  Loprevite M, et al. A Oncol Res, 2005, 15(1), 39-48.
-  Gediya LK, et al. Bioorg Med Chem, 2008, 16(6), 3352-3360.
|In vitro||DMSO||54 mg/mL (200.51 mM)|
|In vivo||Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5% DMSO+40% PEG 300+ddH2O
For best results, use promptly after mixing.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
|Synonyms||PD-123654, GOE-5549, Acetyldinaline|
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