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Tacedinaline (CI994) HDAC inhibitor

Name: Tacedinaline (CI994)
Brand: Selleck Chemicals
SKU: S2818
Availability: InStock
Rating: 5 (42 reviews)

Cat.No.S2818

Tacedinaline (CI994, PD-123654, GOE-5549, Acetyldinaline) is a selective class I HDAC inhibitor with IC50 of 0.9, 0.9, 1.2, and >20 μM for human HDAC 1, 2, 3, and 8, respectively. Phase 3.

Chemical Structure

Molecular Weight: 269.3

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.74%

99.74

Related Targets	Epigenetic Reader Domain JAK Histone Methyltransferase AMPK PKC PARP HIF Histone Acetyltransferase Aurora Kinase Sirtuin PRMT Histone Demethylase EZH1/2 DNA Methyltransferase Pim LSD1 JMJD G9a/GLP MLL NSD FTO MicroRNA PAD SETD SMYD DOT1 COMT WDR5 HNMT SAM MTase
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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Incubation Time	Activity Description
human MDA-MB-231 cells	Growth inhibition assay	5 days	Growth inhibition of human MDA-MB-231 cells after 5 days by MTT assay, GI50=0.17 μM
human PC3 cells	Growth inhibition assay	5 days	Growth inhibition of human PC3 cells after 5 days by MTT assay, GI50=0.29 μM
human HCT116 cells	Cytotoxic assay		Cytotoxicity against human HCT116 cells by MTT assay, IC50=4 μM
293T cells	Function assay		Inhibition of HDAC in 293T cells, IC50=6 μM
human LNCap	Proliferation assay		Antiproliferative activity against human LNCap by MTT assay， IC50=7.4 μM
human NCI-H661 cells	Proliferation assay	48 h	Antiproliferative activity against human NCI-H661 cells after 48 hrs, IC50=20 μM
human NCI-H661 cells	Function assay	6 h	Inhibition of HDAC in human NCI-H661 cells assessed as histone H4 acetylation after 6 hrs by Western blot analysis
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	269.3	Formula	C₁₅H₁₅N₃O₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	112522-64-2	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	PD-123654, GOE-5549, Acetyldinaline			Smiles	CC(=O)NC1=CC=C(C=C1)C(=O)NC2=CC=CC=C2N

Solubility

In vitro
Batch:

DMSO : 54 mg/mL (200.51 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 55%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (18.57mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG 300, mix evenly to clarify it; then continue to add 550 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	HDAC1 ^[1] (Cell-free assay) 0.9 μM HDAC2 ^[1] (Cell-free assay) 0.9 μM HDAC3 ^[1] (Cell-free assay) 1.2 μM
In vitro	CI-994 (< 160 mM) shows cytostatic effect with concomitant increase at G0/G1 phase, a reduction at S phase level and increased apoptosis in A-549 and LX-1 cells. ^[2] CI-994 inhibits growth of LNCaP cell with IC50 of 7.4 μM. ^[3] CI-994 exerts activity against several tumor cell lines with greater cytotoxicity against the solid tumors relative to both the leukemia and normal fibroblast cell lines. ^[4] CI-994 inhibits growth of rat leukemia BCLO cells with IC50 of 2.5 μM. ^[5]
In vivo	CI-994 exerts demonstrated antitumor activity against several tumor models, including the chemo-resistant mouse pancreatic ductal carcinoma as well as the human prostate tumor model LNCaP. ^[4]
References	[1] https://pubmed.ncbi.nlm.nih.gov/17941625/ [2] https://pubmed.ncbi.nlm.nih.gov/15839304/ [3] https://pubmed.ncbi.nlm.nih.gov/18166465/ [4] https://pubmed.ncbi.nlm.nih.gov/9157069/ [5] https://pubmed.ncbi.nlm.nih.gov/18497959/

Applications

Methods	Biomarkers	Images	PMID
Growth inhibition assay	Cell viability		30108928

Tech Support

Handling Instructions

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