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MG132 Proteasome inhibitor

Name: MG132
Brand: Selleck Chemicals
SKU: S2619
Availability: InStock
Rating: 5 (2386 reviews)

Cat.No.S2619

MG132 ((S,R,S)-(-)-MG132, Z-Leu-D-Leu-Leu-al) is a potent proteasome (ChTL, TL, and PGPH) inhibitor. This compound also inhibits calpain (IC50=1.2 μM). It can be used to induce animal models of Parkinson’s disease.

Chemical Structure

Molecular Weight: 475.62

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Selleck's MG-132 had a noticeably greater effect when compared with similar products from Sigma: This is directly due to using the (S, R, S) - (-) - 2 configuration.

P21 protein can be degraded through ubiquitylation pathway. Inhibition of proteasome activities results in the accumulation of P21 protein, therefore, the more powerful the inhibiting effect, the more P21 protein is accumulated. (testing protein: P21; Cell line: Hela; treatment condition: final concentration of MG-132 is 5μM, with a 6 hour treatment time)

Quality Control

Batch: Purity: 99.69%

99.69

Products Often Used Together with MG132

Cycloheximide

Cycloheximide is a protein synthesis inhibitor. It is used in combination with this compound to investigate whether the protein changes are due to the synthesis pathway or the proteasome degradation pathway.

Epoxomicin (BU-4061T)

Epoxomicin is a highly selective and irreversible proteasome inhibitor, which is used in conjunction with it to compare the efficacy differences of different proteasome inhibitors.

Cycloheximide

It and Cycloheximide co-treatment rescues the DM1 protein from degradation in HEK293 cells.

Y-27632 Dihydrochloride

This compound and Y-27632 2HCl treatment inhibits protrusion and increases maturation-promoting factor (MPF) activities, whereas mitogen-activated protein kinase (MAPK) activity remains unchanged in oocytes.

Related Targets	HDAC Caspase Secretase MMP HCV Protease Cysteine Protease DPP Tyrosinase HIV Protease Serine Protease
Other Proteasome Products	Epoxomicin (BU-4061T) Celastrol Oprozomib ONX-0914 (PR-957) Delanzomib VR23 Marizomib (Salinosporamide A) PI-1840 Isoginkgetin S5a/PSMD4 Antibody [L2F5]

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
LP-1	Apoptosis Assay	300 nM	24 h	DMSO	Induces apoptosis by increasing cleaved PARP level and reducing Mcl-1 protein level	15958589
PC3	Growth Inhibition Assay	20 μM	48 h	DMSO	IC50=0.6 μM	16686537
PC12	Function Assay	100 μM	24 h	DMSO	Inhibits 6-OHDA- and H2O2-induced cytotoxicity	17158454
LPS-stimulated RAW264.7 cells	Function Assay			DMSO	Inhibits NF-kappaB DNA binding	17407277
OCI-Ly3	Function Assay	10 μM	4 h	DMSO	Induces human IkappaBalpha stabilization with EC50 of 1 μM	18024113
A2780 cDDP	Apoptosis Assay		24 h	DMSO	Induces apoptosis by inhibiting PTEN degradation	17684018
COS-7	Cytotoxic Assay	10 μM		DMSO	IC50<10 μM	18088097
HuH-7	Cytotoxic Assay	10 μM		DMSO	IC50<10 μM	18088097
Hepa-1c1c7	Function Assay	25 μM	6 h	DMSO	Increases Nrf2 protein level	20392544
IPC227F	Cytotoxic Assay	1 μM	48 h	DMSO	IC50=0.077 μM	21924528
Calu6	Function Assay	10 μM	18 h	DMSO	Significantly accumulates frataxin precursor	23506486
IFN-gamma-induced RAW264.7	Function Assay			DMSO	Inhibits nitric oxide production with IC50 of 0.077 μM	22277277
HEK-293	Kinase Assay	2 and 20 μM	2 h	DMSO	Inhibits ChT-L activity with IC50 of 9 nM	23540790
MCF10A	Growth Inhibition Assay	1 μM	72 h	DMSO	IC50=0.29 μM	24153206
MCF-7	Growth Inhibition Assay	1 μM	72 h	DMSO	IC50=0.13 μM	24153206
HeLa	Kinase Assay	10 μM	1 h	DMSO	Induces PARP cleavage by inhibiting proseasome activity	24321833
MDA-MB-231	Growth Inhibition Assay	1 μM	72 h	DMSO	IC50=0.18 μM	24153206
293T	Cytotoxic Assay	10 μM	72 h	DMSO	IC50<2 μM	24625088
293T	Function Assay	10 μM	24 h	DMSO	Moderately induces heat-shock	24625088
NCI-H929	Cytotoxic Assay	1 μM	72 h	DMSO	IC50=0.17 μM	24625088
A-549	Cytotoxic Assay	40 μM	48 h	DMSO	IC50<10 μM	24697496
MCF-7	Cytotoxic Assay	40 μM	48 h	DMSO	IC50=7.3 μM	24697496
SW-480	Cytotoxic Assay	40 μM	48 h	DMSO	IC50=4 μM	24697496
HL-60	Cytotoxic Assay	40 μM	48 h	DMSO	IC50<10 μM	24697496
SMMC-7721	Cytotoxic Assay	40 μM	48 h	DMSO	IC50=7.1 μM	24697496
LPS-stimulated RAW264.7 cells	Function Assay	25 μM		DMSO	Inhibits nitric oxide production with IC50 of 0.1 μM	24697496
lymphoblastoid	Function assay				IC50 = 0.002 μM	17996987
LCL	Function assay		12 h		IC50 = 0.002 μM	20687609
HL-60	Function assay				IC50 = 0.068 μM	15780623
IPC227F	Function assay		1 h		IC50 = 0.077 μM	16220988
RAW264.7	Antiinflammatory activity assay		18 h		IC50 = 0.08 μM	22620677
RAW264.7	Antiinflammatory activity assay		18 h		IC50 = 0.1 μM	23819871
RAW264.7	Function assay				IC50 = 0.1 μM	24597894
RAW264.7	Function assay		18 h		IC50 = 0.1 μM	24219809
RAW264.7	Function assay		18 h		IC50 = 0.15 μM	23327668
RAW264.7	Function assay		18 h		IC50 = 0.15 μM	26757019
RAW264.7	Antiinflammatory activity assay		18 h		IC50 = 0.18 μM	27203291
RAW264.7	Antiinflammatory activity assay		1 h		IC50 = 0.2 μM	29338260
MDA-MB-231	Cytotoxicity assay				IC50 = 0.25 μM	18598018
MCF7	Cytotoxicity assay				IC50 = 0.3 μM	18598018
RAW264.7	Function assay		18 h		IC50 = 0.35 μM	21044847
RAW264.7	Antiinflammatory activity assay				IC50 = 0.439 μM	21561060
HEK293	Function assay		2 h		IC50 = 0.6 μM	23540790
RAW264.7	Toxicity assay				IC50 = 0.63 μM	22858297
lymphoblastoid	Function assay				IC50 = 1.077 μM	17996987
LCL	Function assay		12 h		IC50 = 1.077 μM	20687609
HL-60	Function assay				IC50 = 1.4 μM	15780623
HL-60	Function assay				IC50 = 4.5 μM	15780623
C8166	Growth Inhibition Assay				IC50=345.338 μM	SANGER
P30-OHK	Growth Inhibition Assay				IC50=283.847 μM	SANGER
NCI-H1838	Growth Inhibition Assay				IC50=271.456 μM	SANGER
THP-1	Growth Inhibition Assay				IC50=264.738 μM	SANGER
MS-1	Growth Inhibition Assay				IC50=239.121 μM	SANGER
REH	Growth Inhibition Assay				IC50=236.626 μM	SANGER
TE-441-T	Growth Inhibition Assay				IC50=199.739 μM	SANGER
TALL-1	Growth Inhibition Assay				IC50=198.304 μM	SANGER
SF539	Growth Inhibition Assay				IC50=192.728 μM	SANGER
NCI-H187	Growth Inhibition Assay				IC50=190.064 μM	SANGER
NCI-H226	Growth Inhibition Assay				IC50=188.866 μM	SANGER
IMR-5	Growth Inhibition Assay				IC50=181.571 μM	SANGER
NCI-H1417	Growth Inhibition Assay				IC50=180.331 μM	SANGER
TUR	Growth Inhibition Assay				IC50=167.87 μM	SANGER
NCI-H1770	Growth Inhibition Assay				IC50=156.279 μM	SANGER
MDA-MB-134-VI	Growth Inhibition Assay				IC50=148.268 μM	SANGER
NB14	Growth Inhibition Assay				IC50=147.082 μM	SANGER
EW-12	Growth Inhibition Assay				IC50=144.699 μM	SANGER
SUP-T1	Growth Inhibition Assay				IC50=143.708 μM	SANGER
KP-N-YS	Growth Inhibition Assay				IC50=139.736 μM	SANGER
BE-13	Growth Inhibition Assay				IC50=134.044 μM	SANGER
NCI-H510A	Growth Inhibition Assay				IC50=127.32 μM	SANGER
DMS-153	Growth Inhibition Assay				IC50=121.745 μM	SANGER
SK-MEL-1	Growth Inhibition Assay				IC50=118.009 μM	SANGER
DMS-79	Growth Inhibition Assay				IC50=117.382 μM	SANGER
DG-75	Growth Inhibition Assay				IC50=113.352 μM	SANGER
MN-60	Growth Inhibition Assay				IC50=109.69 μM	SANGER
WSU-NHL	Growth Inhibition Assay				IC50=109.671 μM	SANGER
BV-173	Growth Inhibition Assay				IC50=108.728 μM	SANGER
EW-22	Growth Inhibition Assay				IC50=106.19 μM	SANGER
NCI-H889	Growth Inhibition Assay				IC50=104.242 μM	SANGER
NCI-H446	Growth Inhibition Assay				IC50=99.7588 μM	SANGER
NCI-H720	Growth Inhibition Assay				IC50=98.4061 μM	SANGER
NB7	Growth Inhibition Assay				IC50=95.7786 μM	SANGER
EW-3	Growth Inhibition Assay				IC50=94.9081 μM	SANGER
ECC4	Growth Inhibition Assay				IC50=93.8269 μM	SANGER
KY821	Growth Inhibition Assay				IC50=91.6551 μM	SANGER
RPMI-8226	Growth Inhibition Assay				IC50=84.2107 μM	SANGER
LU-134-A	Growth Inhibition Assay				IC50=83.1351 μM	SANGER
NCI-H716	Growth Inhibition Assay				IC50=82.9075 μM	SANGER
RS4-11	Growth Inhibition Assay				IC50=82.2505 μM	SANGER
COLO-668	Growth Inhibition Assay				IC50=81.5884 μM	SANGER
NCI-H2171	Growth Inhibition Assay				IC50=79.4136 μM	SANGER
NCI-H526	Growth Inhibition Assay				IC50=78.5877 μM	SANGER
NCI-H322M	Growth Inhibition Assay				IC50=78.3709 μM	SANGER
HCC1187	Growth Inhibition Assay				IC50=77.6122 μM	SANGER
SK-NEP-1	Growth Inhibition Assay				IC50=75.0754 μM	SANGER
DOHH-2	Growth Inhibition Assay				IC50=74.9514 μM	SANGER
NCI-H1155	Growth Inhibition Assay				IC50=74.9367 μM	SANGER
HCC1599	Growth Inhibition Assay				IC50=74.8732 μM	SANGER
RPMI-8866	Growth Inhibition Assay				IC50=74.7509 μM	SANGER
ATN-1	Growth Inhibition Assay				IC50=74.505 μM	SANGER
NCI-H1436	Growth Inhibition Assay				IC50=72.7456 μM	SANGER
SCC-15	Growth Inhibition Assay				IC50=72.34 μM	SANGER
NCI-H82	Growth Inhibition Assay				IC50=71.9507 μM	SANGER
NCI-H1694	Growth Inhibition Assay				IC50=71.032 μM	SANGER
KARPAS-299	Growth Inhibition Assay				IC50=70.4447 μM	SANGER
NB5	Growth Inhibition Assay				IC50=70.3468 μM	SANGER
MHH-CALL-2	Growth Inhibition Assay				IC50=70.06 μM	SANGER
UACC-812	Growth Inhibition Assay				IC50=69.5209 μM	SANGER
NCI-H1522	Growth Inhibition Assay				IC50=69.0047 μM	SANGER
U-698-M	Growth Inhibition Assay				IC50=68.5687 μM	SANGER
SK-MEL-2	Growth Inhibition Assay				IC50=63.9892 μM	SANGER
TGBC24TKB	Growth Inhibition Assay				IC50=62.1491 μM	SANGER
LOXIMVI	Growth Inhibition Assay				IC50=61.8266 μM	SANGER
NCI-H2196	Growth Inhibition Assay				IC50=61.231 μM	SANGER
SCH	Growth Inhibition Assay				IC50=60.9638 μM	SANGER
RPMI-8402	Growth Inhibition Assay				IC50=58.9526 μM	SANGER
NCI-H1395	Growth Inhibition Assay				IC50=58.8172 μM	SANGER
KP-N-RT-BM-1	Growth Inhibition Assay				IC50=56.6638 μM	SANGER
SW962	Growth Inhibition Assay				IC50=54.8357 μM	SANGER
TC-YIK	Growth Inhibition Assay				IC50=50.0363 μM	SANGER
A3-KAW	Growth Inhibition Assay				IC50=49.8061 μM	SANGER
HT	Growth Inhibition Assay				IC50=45.7007 μM	SANGER
SW954	Growth Inhibition Assay				IC50=47.4128 μM	SANGER
CW-2	Growth Inhibition Assay				IC50=44.8342 μM	SANGER
EoL-1-	Growth Inhibition Assay				IC50=44.1568 μM	SANGER
MPP-89	Growth Inhibition Assay				IC50=42.5875 μM	SANGER
MV-4-11	Growth Inhibition Assay				IC50=42.9067 μM	SANGER
SBC-1	Growth Inhibition Assay				IC50=42.1806 μM	SANGER
MLMA	Growth Inhibition Assay				IC50=41.7595 μM	SANGER
DEL	Growth Inhibition Assay				IC50=41.4405 μM	SANGER
LB996-RCC	Growth Inhibition Assay				IC50=40.8716 μM	SANGER
NCI-H64	Growth Inhibition Assay				IC50=40.0948 μM	SANGER
MHH-PREB-1	Growth Inhibition Assay				IC50=39.7388 μM	SANGER
NCI-SNU-16	Growth Inhibition Assay				IC50=38.5653 μM	SANGER
LC-2-ad	Growth Inhibition Assay				IC50=39.4337 μM	SANGER
RCC10RGB	Growth Inhibition Assay				IC50=38.2083 μM	SANGER
NCI-H1299	Growth Inhibition Assay				IC50=38.1603 μM	SANGER
K-562	Growth Inhibition Assay				IC50=37.0864 μM	SANGER
CGTH-W-1	Growth Inhibition Assay				IC50=36.9048 μM	SANGER
KP-N-YN	Growth Inhibition Assay				IC50=34.9057 μM	SANGER
TE-11	Growth Inhibition Assay				IC50=34.4822 μM	SANGER
L-428	Growth Inhibition Assay				IC50=34.7536 μM	SANGER
Ramos-2G6-4C10	Growth Inhibition Assay				IC50=34.2993 μM	SANGER
EW-13	Growth Inhibition Assay				IC50=33.8969 μM	SANGER
NB69	Growth Inhibition Assay				IC50=33.6454 μM	SANGER
EW-24	Growth Inhibition Assay				IC50=32.3474 μM	SANGER
L-363	Growth Inhibition Assay				IC50=32.2144 μM	SANGER
SNU-C1	Growth Inhibition Assay				IC50=31.3126 μM	SANGER
JAR	Growth Inhibition Assay				IC50=31.3799 μM	SANGER
NCI-H2107	Growth Inhibition Assay				IC50=31.2338 μM	SANGER
NB6	Growth Inhibition Assay				IC50=30.9503 μM	SANGER
SIG-M5	Growth Inhibition Assay				IC50=30.7952 μM	SANGER
LNCaP-Clone-FGC	Growth Inhibition Assay				IC50=29.9281 μM	SANGER
COLO-800	Growth Inhibition Assay				IC50=28.2956 μM	SANGER
ETK-1	Growth Inhibition Assay				IC50=28.3446 μM	SANGER
LU-165	Growth Inhibition Assay				IC50=28.0861 μM	SANGER
COR-L279	Growth Inhibition Assay				IC50=26.7922 μM	SANGER
U-87-MG	Growth Inhibition Assay				IC50=26.7644 μM	SANGER
NCI-H1963	Growth Inhibition Assay				IC50=26.0623 μM	SANGER
ST486	Growth Inhibition Assay				IC50=25.1113 μM	SANGER
LS-123	Growth Inhibition Assay				IC50=24.9012 μM	SANGER
NB1	Growth Inhibition Assay				IC50=24.6939 μM	SANGER
CPC-N	Growth Inhibition Assay				IC50=24.0201 μM	SANGER
EHEB	Growth Inhibition Assay				IC50=21.6731 μM	SANGER
CHP-126	Growth Inhibition Assay				IC50=21.6181 μM	SANGER
GR-ST	Growth Inhibition Assay				IC50=21.417 μM	SANGER
NCI-H345	Growth Inhibition Assay				IC50=21.0643 μM	SANGER
RPMI-6666	Growth Inhibition Assay				IC50=21.3602 μM	SANGER
HDLM-2	Growth Inhibition Assay				IC50=20.8821 μM	SANGER
NCI-H1304	Growth Inhibition Assay				IC50=21.0311 μM	SANGER
IM-9	Growth Inhibition Assay				IC50=20.6224 μM	SANGER
P31-FUJ	Growth Inhibition Assay				IC50=20.4753 μM	SANGER
HL-60	Growth Inhibition Assay				IC50=20.1965 μM	SANGER
KMOE-2	Growth Inhibition Assay				IC50=19.6482 μM	SANGER
J-RT3-T3-5	Growth Inhibition Assay				IC50=19.4066 μM	SANGER
MOLT-4	Growth Inhibition Assay				IC50=18.8208 μM	SANGER
Daudi	Growth Inhibition Assay				IC50=18.9231 μM	SANGER
CAL-148	Growth Inhibition Assay				IC50=17.9796 μM	SANGER
HCC2157	Growth Inhibition Assay				IC50=17.4755 μM	SANGER
LS-1034	Growth Inhibition Assay				IC50=17.6818 μM	SANGER
BOKU	Growth Inhibition Assay				IC50=15.9608 μM	SANGER
GOTO	Growth Inhibition Assay				IC50=16.9617 μM	SANGER
COLO-824	Growth Inhibition Assay				IC50=14.7994 μM	SANGER
BC-3	Growth Inhibition Assay				IC50=15.398 μM	SANGER
MSTO-211H	Growth Inhibition Assay				IC50=14.7448 μM	SANGER
JVM-2	Growth Inhibition Assay				IC50=14.7735 μM	SANGER
DB	Growth Inhibition Assay				IC50=13.9885 μM	SANGER
697	Growth Inhibition Assay				IC50=14.4511 μM	SANGER
TE-12	Growth Inhibition Assay				IC50=13.6994 μM	SANGER
NCI-N87	Growth Inhibition Assay				IC50=13.736 μM	SANGER
EB2	Growth Inhibition Assay				IC50=13.8505 μM	SANGER
MHH-NB-11	Growth Inhibition Assay				IC50=13.2902 μM	SANGER
KU812	Growth Inhibition Assay				IC50=13.6124 μM	SANGER
NB13	Growth Inhibition Assay				IC50=12.9783 μM	SANGER
MRK-nu-1	Growth Inhibition Assay				IC50=13.195 μM	SANGER
COR-L88	Growth Inhibition Assay				IC50=12.9382 μM	SANGER
K5	Growth Inhibition Assay				IC50=12.9462 μM	SANGER
KARPAS-422	Growth Inhibition Assay				IC50=12.7496 μM	SANGER
LU-139	Growth Inhibition Assay				IC50=12.9026 μM	SANGER
NOS-1	Growth Inhibition Assay				IC50=12.0345 μM	SANGER
AM-38	Growth Inhibition Assay				IC50=12.5633 μM	SANGER
A498	Growth Inhibition Assay				IC50=12.7069 μM	SANGER
SK-MM-2	Growth Inhibition Assay				IC50=11.8061 μM	SANGER
NCI-H524	Growth Inhibition Assay				IC50=11.9822 μM	SANGER
EW-11	Growth Inhibition Assay				IC50=11.5186 μM	SANGER
TGBC1TKB	Growth Inhibition Assay				IC50=11.4102 μM	SANGER
NCI-H1648	Growth Inhibition Assay				IC50=11.0965 μM	SANGER
SR	Growth Inhibition Assay				IC50=11.0185 μM	SANGER
EC-GI-10	Growth Inhibition Assay				IC50=10.7031 μM	SANGER
SCLC-21H	Growth Inhibition Assay				IC50=10.6596 μM	SANGER
NCI-SNU-1	Growth Inhibition Assay				IC50=10.4969 μM	SANGER
CA46	Growth Inhibition Assay				IC50=10.361 μM	SANGER
NH-12	Growth Inhibition Assay				IC50=10.2433 μM	SANGER
CTV-1	Growth Inhibition Assay				IC50=10.0979 μM	SANGER
IST-MEL1	Growth Inhibition Assay				IC50=9.74198 μM	SANGER
JiyoyeP-2003	Growth Inhibition Assay				IC50=9.29317 μM	SANGER
SJSA-1	Growth Inhibition Assay				IC50=9.09091 μM	SANGER
MC116	Growth Inhibition Assay				IC50=8.9794 μM	SANGER
D-283MED	Growth Inhibition Assay				IC50=8.91719 μM	SANGER
OMC-1	Growth Inhibition Assay				IC50=8.52191 μM	SANGER
RL95-2	Growth Inhibition Assay				IC50=8.10842 μM	SANGER
NB10	Growth Inhibition Assay				IC50=7.99556 μM	SANGER
MZ1-PC	Growth Inhibition Assay				IC50=7.86558 μM	SANGER
LB1047-RCC	Growth Inhibition Assay				IC50=7.72231 μM	SANGER
SK-N-DZ	Growth Inhibition Assay				IC50=7.71089 μM	SANGER
NOMO-1	Growth Inhibition Assay				IC50=7.24254 μM	SANGER
SHP-77	Growth Inhibition Assay				IC50=7.2243 μM	SANGER
OCI-AML2	Growth Inhibition Assay				IC50=7.00326 μM	SANGER
NALM-6	Growth Inhibition Assay				IC50=6.86754 μM	SANGER
KASUMI-1	Growth Inhibition Assay				IC50=6.84787 μM	SANGER
EM-2	Growth Inhibition Assay				IC50=6.67099 μM	SANGER
NMC-G1	Growth Inhibition Assay				IC50=6.71895 μM	SANGER
SK-N-FI	Growth Inhibition Assay				IC50=6.45854 μM	SANGER
U-266	Growth Inhibition Assay				IC50=6.51926 μM	SANGER
HUTU-80	Growth Inhibition Assay				IC50=6.36897 μM	SANGER
TGW	Growth Inhibition Assay				IC50=6.45593 μM	SANGER
A4-Fuk	Growth Inhibition Assay				IC50=6.08118 μM	SANGER
NCI-H1581	Growth Inhibition Assay				IC50=6.23849 μM	SANGER
ARH-77	Growth Inhibition Assay				IC50=5.67087 μM	SANGER
ALL-PO	Growth Inhibition Assay				IC50=5.63795 μM	SANGER
GCIY	Growth Inhibition Assay				IC50=5.47236 μM	SANGER
Calu-6	Growth Inhibition Assay				IC50=5.622 μM	SANGER
SIMA	Growth Inhibition Assay				IC50=5.30765 μM	SANGER
RL	Growth Inhibition Assay				IC50=5.37541 μM	SANGER
RKO	Growth Inhibition Assay				IC50=5.23422 μM	SANGER
NCI-H209	Growth Inhibition Assay				IC50=5.19352 μM	SANGER
BT-474	Growth Inhibition Assay				IC50=5.23102 μM	SANGER
EB-3	Growth Inhibition Assay				IC50=5.03041 μM	SANGER
GT3TKB	Growth Inhibition Assay				IC50=5.17662 μM	SANGER
HAL-01	Growth Inhibition Assay				IC50=4.57034 μM	SANGER
LP-1	Growth Inhibition Assay				IC50=4.72371 μM	SANGER
BC-1	Growth Inhibition Assay				IC50=5.00447 μM	SANGER
LB647-SCLC	Growth Inhibition Assay				IC50=4.56308 μM	SANGER
ES5	Growth Inhibition Assay				IC50=4.40255 μM	SANGER
ES3	Growth Inhibition Assay				IC50=4.32824 μM	SANGER
YT	Growth Inhibition Assay				IC50=4.35424 μM	SANGER
NCI-H1650	Growth Inhibition Assay				IC50=4.15797 μM	SANGER
D-336MG	Growth Inhibition Assay				IC50=4.01166 μM	SANGER
TE-6	Growth Inhibition Assay				IC50=3.94561 μM	SANGER
NKM-1	Growth Inhibition Assay				IC50=3.77378 μM	SANGER
COLO-320-HSR	Growth Inhibition Assay				IC50=3.75634 μM	SANGER
SKM-1	Growth Inhibition Assay				IC50=3.71376 μM	SANGER
KMS-12-PE	Growth Inhibition Assay				IC50=3.67723 μM	SANGER
SNB75	Growth Inhibition Assay				IC50=3.60103 μM	SANGER
NB12	Growth Inhibition Assay				IC50=3.59888 μM	SANGER
MEG-01	Growth Inhibition Assay				IC50=3.5968 μM	SANGER
HCC2218	Growth Inhibition Assay				IC50=3.46937 μM	SANGER
NCI-H748	Growth Inhibition Assay				IC50=3.44266 μM	SANGER
RH-1	Growth Inhibition Assay				IC50=3.38748 μM	SANGER
SCC-3	Growth Inhibition Assay				IC50=3.10848 μM	SANGER
ES4	Growth Inhibition Assay				IC50=3.06111 μM	SANGER
KG-1	Growth Inhibition Assay				IC50=2.88791 μM	SANGER
SW982	Growth Inhibition Assay				IC50=2.5148 μM	SANGER
MFM-223	Growth Inhibition Assay				IC50=2.50871 μM	SANGER
HCE-T	Growth Inhibition Assay				IC50=2.44883 μM	SANGER
ECC12	Growth Inhibition Assay				IC50=2.3794 μM	SANGER
KM-H2	Growth Inhibition Assay				IC50=2.29043 μM	SANGER
MC-CAR	Growth Inhibition Assay				IC50=2.2295 μM	SANGER
QIMR-WIL	Growth Inhibition Assay				IC50=2.16762 μM	SANGER
no-11	Growth Inhibition Assay				IC50=2.03104 μM	SANGER
DJM-1	Growth Inhibition Assay				IC50=2.02552 μM	SANGER
EW-18	Growth Inhibition Assay				IC50=2.02337 μM	SANGER
IST-SL2	Growth Inhibition Assay				IC50=2.01252 μM	SANGER
JVM-3	Growth Inhibition Assay				IC50=1.93904 μM	SANGER
TE-15	Growth Inhibition Assay				IC50=1.92373 μM	SANGER
BB30-HNC	Growth Inhibition Assay				IC50=1.72685 μM	SANGER
SU-DHL-1	Growth Inhibition Assay				IC50=1.71281 μM	SANGER
LS-411N	Growth Inhibition Assay				IC50=1.69807 μM	SANGER
NCI-H2141	Growth Inhibition Assay				IC50=1.67317 μM	SANGER
D-502MG	Growth Inhibition Assay				IC50=1.67211 μM	SANGER
NCI-H2227	Growth Inhibition Assay				IC50=1.61561 μM	SANGER
LC-1F	Growth Inhibition Assay				IC50=1.55198 μM	SANGER
NCI-H2081	Growth Inhibition Assay				IC50=1.55196 μM	SANGER
GI-1	Growth Inhibition Assay				IC50=1.47131 μM	SANGER
DMS-114	Growth Inhibition Assay				IC50=1.44539 μM	SANGER
Raji	Growth Inhibition Assay				IC50=1.4185 μM	SANGER
IST-MES1	Growth Inhibition Assay				IC50=1.40208 μM	SANGER
HC-1	Growth Inhibition Assay				IC50=1.3817 μM	SANGER
NCI-H128	Growth Inhibition Assay				IC50=1.35814 μM	SANGER
EW-16	Growth Inhibition Assay				IC50=1.31347 μM	SANGER
HCE-4	Growth Inhibition Assay				IC50=1.30221 μM	SANGER
SW684	Growth Inhibition Assay				IC50=1.28659 μM	SANGER
NCI-H719	Growth Inhibition Assay				IC50=1.27411 μM	SANGER
PSN1	Growth Inhibition Assay				IC50=1.27016 μM	SANGER
IA-LM	Growth Inhibition Assay				IC50=1.24116 μM	SANGER
NCI-H23	Growth Inhibition Assay				IC50=1.2041 μM	SANGER
MFH-ino	Growth Inhibition Assay				IC50=1.17501 μM	SANGER
LU-65	Growth Inhibition Assay				IC50=1.12753 μM	SANGER
KURAMOCHI	Growth Inhibition Assay				IC50=1.12374 μM	SANGER
L-540	Growth Inhibition Assay				IC50=1.11839 μM	SANGER
ES1	Growth Inhibition Assay				IC50=1.07921 μM	SANGER
KGN	Growth Inhibition Assay				IC50=1.05045 μM	SANGER
KARPAS-45	Growth Inhibition Assay				IC50=1.04016 μM	SANGER
EKVX	Growth Inhibition Assay				IC50=1.03442 μM	SANGER
SF126	Growth Inhibition Assay				IC50=0.98971 μM	SANGER
TE-10	Growth Inhibition Assay				IC50=0.9643 μM	SANGER
HCC2998	Growth Inhibition Assay				IC50=0.93854 μM	SANGER
COLO-684	Growth Inhibition Assay				IC50=0.90658 μM	SANGER
ES7	Growth Inhibition Assay				IC50=0.8965 μM	SANGER
KM12	Growth Inhibition Assay				IC50=0.85466 μM	SANGER
Becker	Growth Inhibition Assay				IC50=0.79621 μM	SANGER
CTB-1	Growth Inhibition Assay				IC50=0.77955 μM	SANGER
GB-1	Growth Inhibition Assay				IC50=0.72124 μM	SANGER
SNU-C2B	Growth Inhibition Assay				IC50=0.70739 μM	SANGER
NCI-SNU-5	Growth Inhibition Assay				IC50=0.69545 μM	SANGER
BL-70	Growth Inhibition Assay				IC50=0.69388 μM	SANGER
NCI-H1355	Growth Inhibition Assay				IC50=0.68619 μM	SANGER
LS-513	Growth Inhibition Assay				IC50=0.66751 μM	SANGER
SW872	Growth Inhibition Assay				IC50=0.65729 μM	SANGER
SF268	Growth Inhibition Assay				IC50=0.65641 μM	SANGER
NCI-H2126	Growth Inhibition Assay				IC50=0.62669 μM	SANGER
TE-9	Growth Inhibition Assay				IC50=0.61033 μM	SANGER
NCI-H1882	Growth Inhibition Assay				IC50=0.59036 μM	SANGER
BB65-RCC	Growth Inhibition Assay				IC50=0.56811 μM	SANGER
MONO-MAC-6	Growth Inhibition Assay				IC50=0.53611 μM	SANGER
Mo-T	Growth Inhibition Assay				IC50=0.51762 μM	SANGER
EVSA-T	Growth Inhibition Assay				IC50=0.49911 μM	SANGER
BB49-HNC	Growth Inhibition Assay				IC50=0.49096 μM	SANGER
A704	Growth Inhibition Assay				IC50=0.48692 μM	SANGER
OS-RC-2	Growth Inhibition Assay				IC50=0.47972 μM	SANGER
CCRF-CEM	Growth Inhibition Assay				IC50=0.47674 μM	SANGER
KALS-1	Growth Inhibition Assay				IC50=0.46584 μM	SANGER
TE-1	Growth Inhibition Assay				IC50=0.45119 μM	SANGER
LC4-1	Growth Inhibition Assay				IC50=0.43607 μM	SANGER
NB17	Growth Inhibition Assay				IC50=0.4297 μM	SANGER
8-MG-BA	Growth Inhibition Assay				IC50=0.39837 μM	SANGER
NCI-H1092	Growth Inhibition Assay				IC50=0.38844 μM	SANGER
NCI-H747	Growth Inhibition Assay				IC50=0.37103 μM	SANGER
GDM-1	Growth Inhibition Assay				IC50=0.35104 μM	SANGER
TE-8	Growth Inhibition Assay				IC50=0.34279 μM	SANGER
HH	Growth Inhibition Assay				IC50=0.32897 μM	SANGER
NCCIT	Growth Inhibition Assay				IC50=0.31783 μM	SANGER
KLE	Growth Inhibition Assay				IC50=0.31306 μM	SANGER
LAN-6	Growth Inhibition Assay				IC50=0.313 μM	SANGER
CMK	Growth Inhibition Assay				IC50=0.3112 μM	SANGER
KNS-81-FD	Growth Inhibition Assay				IC50=0.30323 μM	SANGER
MOLT-16	Growth Inhibition Assay				IC50=0.29524 μM	SANGER
K052	Growth Inhibition Assay				IC50=0.2899 μM	SANGER
C2BBe1	Growth Inhibition Assay				IC50=0.27907 μM	SANGER
CP67-MEL	Growth Inhibition Assay				IC50=0.2673 μM	SANGER
MZ7-mel	Growth Inhibition Assay				IC50=0.26374 μM	SANGER
LOUCY	Growth Inhibition Assay				IC50=0.25456 μM	SANGER
TK10	Growth Inhibition Assay				IC50=0.25435 μM	SANGER
RXF393	Growth Inhibition Assay				IC50=0.25015 μM	SANGER
ES8	Growth Inhibition Assay				IC50=0.24763 μM	SANGER
COLO-829	Growth Inhibition Assay				IC50=0.24257 μM	SANGER
LB373-MEL-D	Growth Inhibition Assay				IC50=0.24198 μM	SANGER
HD-MY-Z	Growth Inhibition Assay				IC50=0.23303 μM	SANGER
SK-PN-DW	Growth Inhibition Assay				IC50=0.2319 μM	SANGER
ACN	Growth Inhibition Assay				IC50=0.22638 μM	SANGER
VA-ES-BJ	Growth Inhibition Assay				IC50=0.22257 μM	SANGER
SK-UT-1	Growth Inhibition Assay				IC50=0.22049 μM	SANGER
LAMA-84	Growth Inhibition Assay				IC50=0.21851 μM	SANGER
EW-1	Growth Inhibition Assay				IC50=0.21047 μM	SANGER
GAK	Growth Inhibition Assay				IC50=0.20534 μM	SANGER
IST-SL1	Growth Inhibition Assay				IC50=0.20094 μM	SANGER
DU-4475	Growth Inhibition Assay				IC50=0.19658 μM	SANGER
LB831-BLC	Growth Inhibition Assay				IC50=0.1934 μM	SANGER
GI-ME-N	Growth Inhibition Assay				IC50=0.19306 μM	SANGER
SK-LMS-1	Growth Inhibition Assay				IC50=0.19303 μM	SANGER
D-392MG	Growth Inhibition Assay				IC50=0.19115 μM	SANGER
HEL	Growth Inhibition Assay				IC50=0.18517 μM	SANGER
MZ2-MEL	Growth Inhibition Assay				IC50=0.17559 μM	SANGER
CESS	Growth Inhibition Assay				IC50=0.1706 μM	SANGER
PF-382	Growth Inhibition Assay				IC50=0.16706 μM	SANGER
A253	Growth Inhibition Assay				IC50=0.16128 μM	SANGER
D-263MG	Growth Inhibition Assay				IC50=0.16006 μM	SANGER
CAS-1	Growth Inhibition Assay				IC50=0.15482 μM	SANGER
NCI-H69	Growth Inhibition Assay				IC50=0.15141 μM	SANGER
KINGS-1	Growth Inhibition Assay				IC50=0.1479 μM	SANGER
TE-5	Growth Inhibition Assay				IC50=0.14278 μM	SANGER
LB2241-RCC	Growth Inhibition Assay				IC50=0.14247 μM	SANGER
HT-144	Growth Inhibition Assay				IC50=0.13895 μM	SANGER
KE-37	Growth Inhibition Assay				IC50=0.13278 μM	SANGER
ONS-76	Growth Inhibition Assay				IC50=0.12935 μM	SANGER
NEC8	Growth Inhibition Assay				IC50=0.11995 μM	SANGER
UACC-257	Growth Inhibition Assay				IC50=0.11927 μM	SANGER
ML-2	Growth Inhibition Assay				IC50=0.11806 μM	SANGER
HOP-62	Growth Inhibition Assay				IC50=0.10949 μM	SANGER
OVCAR-4	Growth Inhibition Assay				IC50=0.10938 μM	SANGER
OPM-2	Growth Inhibition Assay				IC50=0.10474 μM	SANGER
A388	Growth Inhibition Assay				IC50=0.09065 μM	SANGER
no-10	Growth Inhibition Assay				IC50=0.08909 μM	SANGER
LB771-HNC	Growth Inhibition Assay				IC50=0.08036 μM	SANGER
CP66-MEL	Growth Inhibition Assay				IC50=0.075 μM	SANGER
MMAC-SF	Growth Inhibition Assay				IC50=0.07268 μM	SANGER
D-247MG	Growth Inhibition Assay				IC50=0.07209 μM	SANGER
LXF-289	Growth Inhibition Assay				IC50=0.07066 μM	SANGER
BL-41	Growth Inhibition Assay				IC50=0.06947 μM	SANGER
KS-1	Growth Inhibition Assay				IC50=0.06458 μM	SANGER
D-542MG	Growth Inhibition Assay				IC50=0.05957 μM	SANGER
NTERA-S-cl-D1	Growth Inhibition Assay				IC50=0.05772 μM	SANGER
DSH1	Growth Inhibition Assay				IC50=0.05289 μM	SANGER
KNS-42	Growth Inhibition Assay				IC50=0.04441 μM	SANGER
SH-4	Growth Inhibition Assay				IC50=0.0431 μM	SANGER
LB2518-MEL	Growth Inhibition Assay				IC50=0.0376 μM	SANGER
OCUB-M	Growth Inhibition Assay				IC50=0.03737 μM	SANGER
A101D	Growth Inhibition Assay				IC50=0.03279 μM	SANGER
ES6	Growth Inhibition Assay				IC50=0.01306 μM	SANGER
MFH-ino	Growth Inhibition Assay				IC50=0.1111 μM	SANGER
TE-8	Growth Inhibition Assay				IC50=0.27009 μM	SANGER
A101D	Growth Inhibition Assay				IC50=0.30094 μM	SANGER
ES6	Growth Inhibition Assay				IC50=0.33014 μM	SANGER
HT-144	Growth Inhibition Assay				IC50=0.33422 μM	SANGER
RPMI-8226	Growth Inhibition Assay				IC50=0.4231 μM	SANGER
NTERA-S-cl-D1	Growth Inhibition Assay				IC50=0.42592 μM	SANGER
ML-2	Growth Inhibition Assay				IC50=0.47349 μM	SANGER
LB831-BLC	Growth Inhibition Assay				IC50=0.47626 μM	SANGER
BE-13	Growth Inhibition Assay				IC50=0.56207 μM	SANGER
HH	Growth Inhibition Assay				IC50=0.57053 μM	SANGER
PF-382	Growth Inhibition Assay				IC50=0.58379 μM	SANGER
SH-4	Growth Inhibition Assay				IC50=0.60509 μM	SANGER
no-10	Growth Inhibition Assay				IC50=0.60813 μM	SANGER
KINGS-1	Growth Inhibition Assay				IC50=0.62764 μM	SANGER
KE-37	Growth Inhibition Assay				IC50=0.63942 μM	SANGER
TGBC1TKB	Growth Inhibition Assay				IC50=0.68642 μM	SANGER
NCI-H747	Growth Inhibition Assay				IC50=0.71274 μM	SANGER
MONO-MAC-6	Growth Inhibition Assay				IC50=0.7182 μM	SANGER
LC4-1	Growth Inhibition Assay				IC50=0.74747 μM	SANGER
DSH1	Growth Inhibition Assay				IC50=0.75792 μM	SANGER
OPM-2	Growth Inhibition Assay				IC50=0.75837 μM	SANGER
CP66-MEL	Growth Inhibition Assay				IC50=0.83646 μM	SANGER
LB2241-RCC	Growth Inhibition Assay				IC50=0.87076 μM	SANGER
EM-2	Growth Inhibition Assay				IC50=0.87844 μM	SANGER
ONS-76	Growth Inhibition Assay				IC50=0.89904 μM	SANGER
RKO	Growth Inhibition Assay				IC50=0.93176 μM	SANGER
RS4-11	Growth Inhibition Assay				IC50=0.95567 μM	SANGER
KS-1	Growth Inhibition Assay				IC50=0.97175 μM	SANGER
HEL	Growth Inhibition Assay				IC50=1.07025 μM	SANGER
LB771-HNC	Growth Inhibition Assay				IC50=1.07291 μM	SANGER
KGN	Growth Inhibition Assay				IC50=1.17566 μM	SANGER
NMC-G1	Growth Inhibition Assay				IC50=1.18954 μM	SANGER
C2BBe1	Growth Inhibition Assay				IC50=1.20033 μM	SANGER
HCC1599	Growth Inhibition Assay				IC50=1.25589 μM	SANGER
TK10	Growth Inhibition Assay				IC50=1.28397 μM	SANGER
IST-SL1	Growth Inhibition Assay				IC50=1.30754 μM	SANGER
Raji	Growth Inhibition Assay				IC50=1.46531 μM	SANGER
KALS-1	Growth Inhibition Assay				IC50=1.46658 μM	SANGER
BB49-HNC	Growth Inhibition Assay				IC50=1.49911 μM	SANGER
LB996-RCC	Growth Inhibition Assay				IC50=1.55279 μM	SANGER
NCI-H1092	Growth Inhibition Assay				IC50=1.58368 μM	SANGER
DEL	Growth Inhibition Assay				IC50=1.5921 μM	SANGER
HT	Growth Inhibition Assay				IC50=1.70043 μM	SANGER
TE-5	Growth Inhibition Assay				IC50=1.83901 μM	SANGER
RPMI-6666	Growth Inhibition Assay				IC50=1.85897 μM	SANGER
JVM-2	Growth Inhibition Assay				IC50=1.95209 μM	SANGER
Daudi	Growth Inhibition Assay				IC50=2.05459 μM	SANGER
NCI-SNU-5	Growth Inhibition Assay				IC50=2.07059 μM	SANGER
BL-41	Growth Inhibition Assay				IC50=2.11804 μM	SANGER
OMC-1	Growth Inhibition Assay				IC50=2.15065 μM	SANGER
HCC2157	Growth Inhibition Assay				IC50=2.15677 μM	SANGER
MLMA	Growth Inhibition Assay				IC50=2.18576 μM	SANGER
LB373-MEL-D	Growth Inhibition Assay				IC50=2.23667 μM	SANGER
KURAMOCHI	Growth Inhibition Assay				IC50=2.25183 μM	SANGER
697	Growth Inhibition Assay				IC50=2.26226 μM	SANGER
KARPAS-422	Growth Inhibition Assay				IC50=2.30393 μM	SANGER
CCRF-CEM	Growth Inhibition Assay				IC50=2.31255 μM	SANGER
A3-KAW	Growth Inhibition Assay				IC50=2.38186 μM	SANGER
KARPAS-299	Growth Inhibition Assay				IC50=2.38967 μM	SANGER
LAMA-84	Growth Inhibition Assay				IC50=2.45622 μM	SANGER
SCC-3	Growth Inhibition Assay				IC50=2.47955 μM	SANGER
SW872	Growth Inhibition Assay				IC50=2.60022 μM	SANGER
KNS-81-FD	Growth Inhibition Assay				IC50=2.67382 μM	SANGER
NCCIT	Growth Inhibition Assay				IC50=2.67795 μM	SANGER
ACN	Growth Inhibition Assay				IC50=2.84394 μM	SANGER
CAS-1	Growth Inhibition Assay				IC50=2.86126 μM	SANGER
GB-1	Growth Inhibition Assay				IC50=2.88421 μM	SANGER
EW-16	Growth Inhibition Assay				IC50=2.98023 μM	SANGER
LB647-SCLC	Growth Inhibition Assay				IC50=3.0086 μM	SANGER
KM-H2	Growth Inhibition Assay				IC50=3.14413 μM	SANGER
Becker	Growth Inhibition Assay				IC50=3.20254 μM	SANGER
MMAC-SF	Growth Inhibition Assay				IC50=3.21082 μM	SANGER
BC-1	Growth Inhibition Assay				IC50=3.24287 μM	SANGER
GR-ST	Growth Inhibition Assay				IC50=3.59266 μM	SANGER
SCC-15	Growth Inhibition Assay				IC50=3.59326 μM	SANGER
KP-N-YS	Growth Inhibition Assay				IC50=3.62126 μM	SANGER
A253	Growth Inhibition Assay				IC50=3.76302 μM	SANGER
JiyoyeP-2003	Growth Inhibition Assay				IC50=3.82083 μM	SANGER
LAN-6	Growth Inhibition Assay				IC50=3.85926 μM	SANGER
VA-ES-BJ	Growth Inhibition Assay				IC50=3.96369 μM	SANGER
MPP-89	Growth Inhibition Assay				IC50=3.98406 μM	SANGER
K-562	Growth Inhibition Assay				IC50=3.9918 μM	SANGER
KASUMI-1	Growth Inhibition Assay				IC50=3.99677 μM	SANGER
THP-1	Growth Inhibition Assay				IC50=4.04835 μM	SANGER
DOHH-2	Growth Inhibition Assay				IC50=4.06592 μM	SANGER
EW-1	Growth Inhibition Assay				IC50=4.19255 μM	SANGER
K5	Growth Inhibition Assay				IC50=4.26164 μM	SANGER
SR	Growth Inhibition Assay				IC50=4.3002 μM	SANGER
NCI-H719	Growth Inhibition Assay				IC50=4.36265 μM	SANGER
A388	Growth Inhibition Assay				IC50=4.39507 μM	SANGER
HCC2218	Growth Inhibition Assay				IC50=4.44812 μM	SANGER
EB2	Growth Inhibition Assay				IC50=4.5114 μM	SANGER
UACC-257	Growth Inhibition Assay				IC50=4.51454 μM	SANGER
LC-1F	Growth Inhibition Assay				IC50=4.63784 μM	SANGER
NB1	Growth Inhibition Assay				IC50=4.8413 μM	SANGER
NCI-H748	Growth Inhibition Assay				IC50=4.90737 μM	SANGER
NALM-6	Growth Inhibition Assay				IC50=4.92582 μM	SANGER
NB7	Growth Inhibition Assay				IC50=5.04589 μM	SANGER
ECC4	Growth Inhibition Assay				IC50=5.05273 μM	SANGER
no-11	Growth Inhibition Assay				IC50=5.16913 μM	SANGER
COLO-824	Growth Inhibition Assay				IC50=5.40415 μM	SANGER
LXF-289	Growth Inhibition Assay				IC50=5.40704 μM	SANGER
MOLT-4	Growth Inhibition Assay				IC50=5.48599 μM	SANGER
NCI-H1355	Growth Inhibition Assay				IC50=5.63865 μM	SANGER
ATN-1	Growth Inhibition Assay				IC50=5.76359 μM	SANGER
NOS-1	Growth Inhibition Assay				IC50=5.85194 μM	SANGER
PSN1	Growth Inhibition Assay				IC50=5.90945 μM	SANGER
NOMO-1	Growth Inhibition Assay				IC50=5.99228 μM	SANGER
NCI-H23	Growth Inhibition Assay				IC50=6.10875 μM	SANGER
LB1047-RCC	Growth Inhibition Assay				IC50=6.3288 μM	SANGER
ES8	Growth Inhibition Assay				IC50=6.36816 μM	SANGER
LS-1034	Growth Inhibition Assay				IC50=6.55658 μM	SANGER
MDA-MB-134-VI	Growth Inhibition Assay				IC50=6.64033 μM	SANGER
MZ1-PC	Growth Inhibition Assay				IC50=6.674 μM	SANGER
ES4	Growth Inhibition Assay				IC50=6.92652 μM	SANGER
SNB75	Growth Inhibition Assay				IC50=7.20588 μM	SANGER
P30-OHK	Growth Inhibition Assay				IC50=7.21282 μM	SANGER
Calu-6	Growth Inhibition Assay				IC50=7.27563 μM	SANGER
MHH-PREB-1	Growth Inhibition Assay				IC50=7.32929 μM	SANGER
DU-4475	Growth Inhibition Assay				IC50=7.57964 μM	SANGER
U-698-M	Growth Inhibition Assay				IC50=7.85992 μM	SANGER
IM-9	Growth Inhibition Assay				IC50=8.12437 μM	SANGER
QIMR-WIL	Growth Inhibition Assay				IC50=8.13337 μM	SANGER
NB5	Growth Inhibition Assay				IC50=8.23226 μM	SANGER
RPMI-8402	Growth Inhibition Assay				IC50=8.27928 μM	SANGER
MZ2-MEL	Growth Inhibition Assay				IC50=8.46677 μM	SANGER
SK-MEL-2	Growth Inhibition Assay				IC50=8.5574 μM	SANGER
NCI-H69	Growth Inhibition Assay				IC50=8.79201 μM	SANGER
CMK	Growth Inhibition Assay				IC50=8.84538 μM	SANGER
MV-4-11	Growth Inhibition Assay				IC50=8.86801 μM	SANGER
MEG-01	Growth Inhibition Assay				IC50=8.88154 μM	SANGER
NCI-H1581	Growth Inhibition Assay				IC50=9.15716 μM	SANGER
MZ7-mel	Growth Inhibition Assay				IC50=9.27307 μM	SANGER
SJSA-1	Growth Inhibition Assay				IC50=9.52951 μM	SANGER
SKM-1	Growth Inhibition Assay				IC50=9.61824 μM	SANGER
YT	Growth Inhibition Assay				IC50=9.83673 μM	SANGER
LS-123	Growth Inhibition Assay				IC50=10.1011 μM	SANGER
NCI-H720	Growth Inhibition Assay				IC50=10.2198 μM	SANGER
NH-12	Growth Inhibition Assay				IC50=10.2432 μM	SANGER
NKM-1	Growth Inhibition Assay				IC50=10.5981 μM	SANGER
HUTU-80	Growth Inhibition Assay				IC50=10.616 μM	SANGER
TUR	Growth Inhibition Assay				IC50=10.8222 μM	SANGER
NB14	Growth Inhibition Assay				IC50=11.7876 μM	SANGER
CESS	Growth Inhibition Assay				IC50=12.0629 μM	SANGER
ES5	Growth Inhibition Assay				IC50=12.1105 μM	SANGER
EB-3	Growth Inhibition Assay				IC50=12.4485 μM	SANGER
SNU-C1	Growth Inhibition Assay				IC50=12.6517 μM	SANGER
CA46	Growth Inhibition Assay				IC50=12.9298 μM	SANGER
NCI-H1304	Growth Inhibition Assay				IC50=13.0111 μM	SANGER
NCI-H1522	Growth Inhibition Assay				IC50=13.2394 μM	SANGER
ST486	Growth Inhibition Assay				IC50=13.3147 μM	SANGER
D-263MG	Growth Inhibition Assay				IC50=13.565 μM	SANGER
L-540	Growth Inhibition Assay				IC50=13.6084 μM	SANGER
LOUCY	Growth Inhibition Assay				IC50=13.7564 μM	SANGER
EW-3	Growth Inhibition Assay				IC50=13.8253 μM	SANGER
HCC1187	Growth Inhibition Assay				IC50=13.8349 μM	SANGER
KU812	Growth Inhibition Assay				IC50=14.0902 μM	SANGER
NCI-H1963	Growth Inhibition Assay				IC50=14.1004 μM	SANGER
SCH	Growth Inhibition Assay				IC50=14.1615 μM	SANGER
NCI-H1417	Growth Inhibition Assay				IC50=14.3226 μM	SANGER
NCI-H2081	Growth Inhibition Assay				IC50=14.5781 μM	SANGER
WSU-NHL	Growth Inhibition Assay				IC50=14.6373 μM	SANGER
CGTH-W-1	Growth Inhibition Assay				IC50=15.0867 μM	SANGER
TE-12	Growth Inhibition Assay				IC50=15.1938 μM	SANGER
OCUB-M	Growth Inhibition Assay				IC50=15.3376 μM	SANGER
ES3	Growth Inhibition Assay				IC50=15.6556 μM	SANGER
GOTO	Growth Inhibition Assay				IC50=15.8269 μM	SANGER
SW962	Growth Inhibition Assay				IC50=15.8866 μM	SANGER
BC-3	Growth Inhibition Assay				IC50=16.566 μM	SANGER
GT3TKB	Growth Inhibition Assay				IC50=17.0105 μM	SANGER
SK-N-DZ	Growth Inhibition Assay				IC50=17.1644 μM	SANGER
LU-65	Growth Inhibition Assay				IC50=17.6506 μM	SANGER
EC-GI-10	Growth Inhibition Assay				IC50=18.0735 μM	SANGER
ARH-77	Growth Inhibition Assay				IC50=18.3741 μM	SANGER
BB65-RCC	Growth Inhibition Assay				IC50=18.5752 μM	SANGER
NCI-H1648	Growth Inhibition Assay				IC50=18.6376 μM	SANGER
SK-NEP-1	Growth Inhibition Assay				IC50=18.7023 μM	SANGER
NCI-H64	Growth Inhibition Assay				IC50=18.944 μM	SANGER
TE-15	Growth Inhibition Assay				IC50=18.9815 μM	SANGER
LNCaP-Clone-FGC	Growth Inhibition Assay				IC50=19.3517 μM	SANGER
ES7	Growth Inhibition Assay				IC50=19.46 μM	SANGER
NCI-H2141	Growth Inhibition Assay				IC50=19.5619 μM	SANGER
U-87-MG	Growth Inhibition Assay				IC50=19.6307 μM	SANGER
GAK	Growth Inhibition Assay				IC50=19.7956 μM	SANGER
TE-9	Growth Inhibition Assay				IC50=20.0459 μM	SANGER
TE-1	Growth Inhibition Assay				IC50=20.8915 μM	SANGER
DMS-79	Growth Inhibition Assay				IC50=20.9557 μM	SANGER
GDM-1	Growth Inhibition Assay				IC50=21.0468 μM	SANGER
NCI-H1650	Growth Inhibition Assay				IC50=21.2129 μM	SANGER
D-283MED	Growth Inhibition Assay				IC50=21.4585 μM	SANGER
HD-MY-Z	Growth Inhibition Assay				IC50=22.3294 μM	SANGER
EW-13	Growth Inhibition Assay				IC50=22.4135 μM	SANGER
8-MG-BA	Growth Inhibition Assay				IC50=22.956 μM	SANGER
A498	Growth Inhibition Assay				IC50=23.2708 μM	SANGER
NCI-H524	Growth Inhibition Assay				IC50=23.8942 μM	SANGER
D-247MG	Growth Inhibition Assay				IC50=23.9489 μM	SANGER
IST-MES1	Growth Inhibition Assay				IC50=24.239 μM	SANGER
NCI-H1838	Growth Inhibition Assay				IC50=24.9878 μM	SANGER
SK-UT-1	Growth Inhibition Assay				IC50=25.1851 μM	SANGER
LS-513	Growth Inhibition Assay				IC50=25.5136 μM	SANGER
MHH-NB-11	Growth Inhibition Assay				IC50=25.9507 μM	SANGER
TALL-1	Growth Inhibition Assay				IC50=26.5085 μM	SANGER
RCC10RGB	Growth Inhibition Assay				IC50=26.5486 μM	SANGER
L-363	Growth Inhibition Assay				IC50=26.6166 μM	SANGER
MC-CAR	Growth Inhibition Assay				IC50=26.6901 μM	SANGER
KNS-42	Growth Inhibition Assay				IC50=26.8177 μM	SANGER
LB2518-MEL	Growth Inhibition Assay				IC50=27.479 μM	SANGER
SF126	Growth Inhibition Assay				IC50=27.6883 μM	SANGER
BV-173	Growth Inhibition Assay				IC50=27.8955 μM	SANGER
C8166	Growth Inhibition Assay				IC50=27.9353 μM	SANGER
SU-DHL-1	Growth Inhibition Assay				IC50=28.9415 μM	SANGER
LP-1	Growth Inhibition Assay				IC50=29.1543 μM	SANGER
SK-PN-DW	Growth Inhibition Assay				IC50=29.2129 μM	SANGER
RL95-2	Growth Inhibition Assay				IC50=29.5637 μM	SANGER
GCIY	Growth Inhibition Assay				IC50=29.7428 μM	SANGER
NCI-H1770	Growth Inhibition Assay				IC50=29.9399 μM	SANGER
KY821	Growth Inhibition Assay				IC50=30.5434 μM	SANGER
NB13	Growth Inhibition Assay				IC50=30.9584 μM	SANGER
BB30-HNC	Growth Inhibition Assay				IC50=31.0823 μM	SANGER
EW-24	Growth Inhibition Assay				IC50=32.3473 μM	SANGER
GI-1	Growth Inhibition Assay				IC50=33.7814 μM	SANGER
OVCAR-4	Growth Inhibition Assay				IC50=34.065 μM	SANGER
HC-1	Growth Inhibition Assay				IC50=34.4958 μM	SANGER
NCI-H209	Growth Inhibition Assay				IC50=34.7578 μM	SANGER
COLO-829	Growth Inhibition Assay				IC50=35.1091 μM	SANGER
NCI-H1395	Growth Inhibition Assay				IC50=35.8459 μM	SANGER
HOP-62	Growth Inhibition Assay				IC50=36.8021 μM	SANGER
NCI-H2171	Growth Inhibition Assay				IC50=36.9848 μM	SANGER
LS-411N	Growth Inhibition Assay				IC50=36.9919 μM	SANGER
ETK-1	Growth Inhibition Assay				IC50=37.05 μM	SANGER
KMOE-2	Growth Inhibition Assay				IC50=37.2234 μM	SANGER
EKVX	Growth Inhibition Assay				IC50=37.3946 μM	SANGER
SF539	Growth Inhibition Assay				IC50=38.6705 μM	SANGER
RL	Growth Inhibition Assay				IC50=38.6818 μM	SANGER
MOLT-16	Growth Inhibition Assay				IC50=38.9944 μM	SANGER
NCI-H128	Growth Inhibition Assay				IC50=39.5567 μM	SANGER
SHP-77	Growth Inhibition Assay				IC50=39.7722 μM	SANGER
EVSA-T	Growth Inhibition Assay				IC50=40.0164 μM	SANGER
DB	Growth Inhibition Assay				IC50=40.4441 μM	SANGER
TE-441-T	Growth Inhibition Assay				IC50=40.7601 μM	SANGER
NCI-SNU-16	Growth Inhibition Assay				IC50=40.7771 μM	SANGER
ALL-PO	Growth Inhibition Assay				IC50=40.8264 μM	SANGER
P31-FUJ	Growth Inhibition Assay				IC50=41.0485 μM	SANGER
EW-24	Growth Inhibition Assay				IC50=41.4224 μM	SANGER
LC-2-ad	Growth Inhibition Assay				IC50=41.7115 μM	SANGER
AM-38	Growth Inhibition Assay				IC50=43.4398 μM	SANGER
COLO-684	Growth Inhibition Assay				IC50=43.4687 μM	SANGER
L-428	Growth Inhibition Assay				IC50=44.6708 μM	SANGER
RXF393	Growth Inhibition Assay				IC50=44.7331 μM	SANGER
MRK-nu-1	Growth Inhibition Assay				IC50=45.9253 μM	SANGER
TE-10	Growth Inhibition Assay				IC50=46.4915 μM	SANGER
Ramos-2G6-4C10	Growth Inhibition Assay				IC50=46.5661 μM	SANGER
NCI-H1694	Growth Inhibition Assay				IC50=46.8105 μM	SANGER
A4-Fuk	Growth Inhibition Assay				IC50=47.7106 μM	SANGER
REH	Growth Inhibition Assay				IC50=47.8793 μM	SANGER
CAL-148	Growth Inhibition Assay				IC50=49.101 μM	SANGER
KARPAS-45	Growth Inhibition Assay				IC50=51.0359 μM	SANGER
NEC8	Growth Inhibition Assay				IC50=51.178 μM	SANGER
NCI-H446	Growth Inhibition Assay				IC50=54.3092 μM	SANGER
SF268	Growth Inhibition Assay				IC50=56.4043 μM	SANGER
EoL-1-cell	Growth Inhibition Assay				IC50=57.0315 μM	SANGER
IST-MEL1	Growth Inhibition Assay				IC50=58.6442 μM	SANGER
OS-RC-2	Growth Inhibition Assay				IC50=58.6841 μM	SANGER
BL-70	Growth Inhibition Assay				IC50=58.8002 μM	SANGER
GI-ME-N	Growth Inhibition Assay				IC50=59.7717 μM	SANGER
SK-LMS-1	Growth Inhibition Assay				IC50=60.4282 μM	SANGER
RH-1	Growth Inhibition Assay				IC50=61.2697 μM	SANGER
D-336MG	Growth Inhibition Assay				IC50=61.2933 μM	SANGER
NCI-H2196	Growth Inhibition Assay				IC50=62.2931 μM	SANGER
IST-SL2	Growth Inhibition Assay				IC50=65.0102 μM	SANGER
DG-75	Growth Inhibition Assay				IC50=65.873 μM	SANGER
SK-MEL-1	Growth Inhibition Assay				IC50=66.8299 μM	SANGER
SK-N-FI	Growth Inhibition Assay				IC50=67.97 μM	SANGER
SCLC-21H	Growth Inhibition Assay				IC50=69.6286 μM	SANGER
KM12	Growth Inhibition Assay				IC50=70.1607 μM	SANGER
TE-6	Growth Inhibition Assay				IC50=71.6266 μM	SANGER
HCE-T	Growth Inhibition Assay				IC50=72.7372 μM	SANGER
ES1	Growth Inhibition Assay				IC50=75.1944 μM	SANGER
SK-MM-2	Growth Inhibition Assay				IC50=75.8461 μM	SANGER
SW954	Growth Inhibition Assay				IC50=78.0688 μM	SANGER
LU-139	Growth Inhibition Assay				IC50=78.4739 μM	SANGER
SBC-1	Growth Inhibition Assay				IC50=79.7008 μM	SANGER
K052	Growth Inhibition Assay				IC50=81.9603 μM	SANGER
SIG-M5	Growth Inhibition Assay				IC50=82.9596 μM	SANGER
TGW	Growth Inhibition Assay				IC50=84.6867 μM	SANGER
LU-134-A	Growth Inhibition Assay				IC50=85.9404 μM	SANGER
OCI-AML2	Growth Inhibition Assay				IC50=86.8607 μM	SANGER
LOXIMVI	Growth Inhibition Assay				IC50=88.1676 μM	SANGER
MC116	Growth Inhibition Assay				IC50=88.8718 μM	SANGER
JAR	Growth Inhibition Assay				IC50=89.0582 μM	SANGER
BT-474	Growth Inhibition Assay				IC50=97.7609 μM	SANGER
IMR-5	Growth Inhibition Assay				IC50=99.7585 μM	SANGER
HL-60	Growth Inhibition Assay				IC50=102.901 μM	SANGER
NCI-H1882	Growth Inhibition Assay				IC50=103.457 μM	SANGER
NB17	Growth Inhibition Assay				IC50=106.528 μM	SANGER
NCI-H2126	Growth Inhibition Assay				IC50=107.635 μM	SANGER
J-RT3-T3-5	Growth Inhibition Assay				IC50=108.33 μM	SANGER
NB69	Growth Inhibition Assay				IC50=112.333 μM	SANGER
JVM-3	Growth Inhibition Assay				IC50=113.512 μM	SANGER
NCI-H226	Growth Inhibition Assay				IC50=114.376 μM	SANGER
CTV-1	Growth Inhibition Assay				IC50=115.234 μM	SANGER
KMS-12-PE	Growth Inhibition Assay				IC50=115.583 μM	SANGER
DMS-153	Growth Inhibition Assay				IC50=117.411 μM	SANGER
NCI-H1436	Growth Inhibition Assay				IC50=118.868 μM	SANGER
DJM-1	Growth Inhibition Assay				IC50=119.978 μM	SANGER
CTB-1	Growth Inhibition Assay				IC50=123.948 μM	SANGER
RPMI-8866	Growth Inhibition Assay				IC50=134.232 μM	SANGER
NB6	Growth Inhibition Assay				IC50=135.081 μM	SANGER
NCI-H510A	Growth Inhibition Assay				IC50=135.852 μM	SANGER
HAL-01	Growth Inhibition Assay				IC50=136.429 μM	SANGER
COLO-668	Growth Inhibition Assay				IC50=136.607 μM	SANGER
NB10	Growth Inhibition Assay				IC50=139.732 μM	SANGER
U-266	Growth Inhibition Assay				IC50=146.561 μM	SANGER
D-502MG	Growth Inhibition Assay				IC50=148.717 μM	SANGER
HDLM-2	Growth Inhibition Assay				IC50=165.958 μM	SANGER
MN-60	Growth Inhibition Assay				IC50=167.261 μM	SANGER
SW684	Growth Inhibition Assay				IC50=182.57 μM	SANGER
COR-L279	Growth Inhibition Assay				IC50=186.961 μM	SANGER
CPC-N	Growth Inhibition Assay				IC50=188.945 μM	SANGER
NCI-SNU-1	Growth Inhibition Assay				IC50=201.087 μM	SANGER
COLO-320-HSR	Growth Inhibition Assay				IC50=209.004 μM	SANGER
NCI-H889	Growth Inhibition Assay				IC50=209.602 μM	SANGER
MHH-CALL-2	Growth Inhibition Assay				IC50=230.298 μM	SANGER
COR-L88	Growth Inhibition Assay				IC50=231.162 μM	SANGER
CHP-126	Growth Inhibition Assay				IC50=237.146 μM	SANGER
NCI-H82	Growth Inhibition Assay				IC50=240.731 μM	SANGER
SUP-T1	Growth Inhibition Assay				IC50=243.062 μM	SANGER
NCI-H345	Growth Inhibition Assay				IC50=246.503 μM	SANGER
LU-165	Growth Inhibition Assay				IC50=247.336 μM	SANGER
DMS-114	Growth Inhibition Assay				IC50=253.811 μM	SANGER
NCI-H716	Growth Inhibition Assay				IC50=259.637 μM	SANGER
SIMA	Growth Inhibition Assay				IC50=312.028 μM	SANGER
NCI-H526	Growth Inhibition Assay				IC50=312.685 μM	SANGER
NH-12	Growth Inhibition Assay				IC50=316.901 μM	SANGER
MS-1	Growth Inhibition Assay				IC50=327.666 μM	SANGER
MFM-223	Growth Inhibition Assay				IC50=340.486 μM	SANGER
EW-11	Growth Inhibition Assay				IC50=348.123 μM	SANGER
KG-1	Growth Inhibition Assay				IC50=387.778 μM	SANGER
NCI-H1155	Growth Inhibition Assay				IC50=414.626 μM	SANGER
NCI-H187	Growth Inhibition Assay				IC50=428.541 μM	SANGER
MSTO-211H	Growth Inhibition Assay				IC50=434.624 μM	SANGER
NCI-H322M	Growth Inhibition Assay				IC50=452.677 μM	SANGER
CW-2	Growth Inhibition Assay				IC50=483.609 μM	SANGER
COLO-800	Growth Inhibition Assay				IC50=525.413 μM	SANGER
EW-12	Growth Inhibition Assay				IC50=557.847 μM	SANGER
EW-18	Growth Inhibition Assay				IC50=672.665 μM	SANGER
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 95 mg/mL (199.73 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 95 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml (5.26mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	475.62	Formula	C₂₆H₄₁N₃O₅	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	1211877-36-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	(S,R,S)-(-)-MG-132, Z-Leu-D-Leu-Leu-al			Smiles	CC(C)CC(C=O)NC(=O)C(CC(C)C)NC(=O)C(CC(C)C)NC(=O)OCC1=CC=CC=C1

Mechanism of Action

Targets/IC₅₀/Ki	ChTL (Cell-free assay) 0.22 μM
In vitro	MG-132 displays >1000 times more activity than ZLLal in inhibiting the ZLLL-MCA-degrading activity of 20S proteasome with IC50 of 100 nM versus 110 μM. This compound also inhibits calpain with IC50 of 1.2 μM. This chemical induces neurite outgrowth in PC12 cells at an optimal concentration of 20 nM, displaying 500 times more potency than ZLLal. This compound (10 μM) potently inhibits TNF-α-induced NF-κB activation, interleukin-8 (IL-8) gene transcription, and IL-8 protein release in A549 cells by inhibition of proteasome-mediated IκBα degradation. This chemical treatment potently induces p53-dependent apoptosis in KIM-2 cells by 26S proteasome inhibition. Unlike BzLLLCOCHO or PS-341, this compound treatment results in weak inhibition of the chymotrypsinlike (CT-L) and peptidylglutamyl peptide hydrolysing (PGPH) activities of the 26S proteasome, whereas multiple myeloma cells (U266 and OPM-2) are more sensitive to induction of apoptosis by this chemical than BzLLLCOCHO. This compound (1 μM) sensitizes TRAIL-resistant prostate cancer cells by activating the AP-1 family members c-Fos and c-Jun, which, in turn, repress the antiapoptotic molecule c-FLIP(L). This chemical significantly enhances the ability of inositol hexakisphosphate (IP6) to reduce cellular metabolic activity in both PC3 and DU145 androgen-independent prostate cancer (AIPCa) cell lines.
Kinase Assay	Measurement of inhibitory activities of MG-132 against 20S proteasome
Kinase Assay	The reaction mixture for the 20S proteasome inhibitory assay contains 0.1 M Tris-acetate, pH 7.0, 20S proteasome, MG-132, and 25 μM substrate dissolved in dimethyl sulfoxide in a final volume of 1 mL. After incubation at 37 °C for 15 minutes, the reaction is stopped by the addition of 0.1 mL of 10% SDS and 0.9 mL of 0.1M Tris acetate, pH 9.0. The fluorescence of the reaction products is measured. To determine the IC50 against 20S proteasome, various concentrations of this compound are included in the assay mixture.
In vivo	Administration of MG-132 effectively rescues the expression levels and plasma membrane localization of dystrophin, β-dystroglycan, α-bdystroglycan, and α-sarcoglycan in skeletal muscle fibers from mdx mice, reduces muscle membrane damage, and ameliorates the histopathological signs of muscular dystrophy. Treatment with this compound significantly reduces immobilization-induced skeletal muscle atrophy in mice, by downregulating the muscle-specific ubiquitin ligases atrogin-1/MAFbx and MuRF-1 mRNA.
References	[1] https://pubmed.ncbi.nlm.nih.gov/8830056/ [2] https://pubmed.ncbi.nlm.nih.gov/9698598/ [3] https://pubmed.ncbi.nlm.nih.gov/11319603/ [4] https://pubmed.ncbi.nlm.nih.gov/16778216/ [5] https://pubmed.ncbi.nlm.nih.gov/17332355/ [6] https://pubmed.ncbi.nlm.nih.gov/18941459/ [7] https://pubmed.ncbi.nlm.nih.gov/14507673/ [8] https://pubmed.ncbi.nlm.nih.gov/21843349/ [9] https://pubmed.ncbi.nlm.nih.gov/20112914/

Applications

Methods	Biomarkers	PMID
Western Blot	Bcr-Abl Ac-histone H3 Cleaved-caspase3 Cleaved-PARP ASCL1 CgA Nrf2 HO-1	26722260
Immunofluorescence	C2GnT-M / NMIIA AGS3 / Vimentin Bak	22525330
Growth inhibition assay	Apoptosis assay Cell viability cell proliferation	20557369
ELISA	IL-8 IL-1beta	11160671