GsMTx4 (Grammostola spatulata mechanotoxin 4, M-theraphotoxin-Gr1a, M-TRTX-Gr1a) is a spider venom peptide that inhibits cationic mechanosensitive channels (MSCs).

Amino-acid Sequence

CAS No. 1209500-46-8

Purity & Quality Control

Batch: Purity: 99.82%

99.82

Chemical Information & Solubility

Storage (From the date of receipt)	3 years-20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

Water : 100 mg/mL

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Biological Activity

Description

GsMTx4 (Grammostola spatulata mechanotoxin 4, M-theraphotoxin-Gr1a, M-TRTX-Gr1a) is a spider venom peptide that inhibits cationic mechanosensitive channels (MSCs).

Targets

MSCs

In vitro
In vitro	Pretreatment of GsMTx4 and BAPTA-AM suppress the Yoda1-induced accumulation of HIF-1alpha, NFATC1, and NFATC3 proteins and also suppress Yoda1-induced VEGF-A expression at the mRNA and protein levels.^[2]
Cell Research	Cell lines	BMDMs, RAW264.7 cells
	Concentrations	5, 10 µM
	Incubation Time	30 min
	Method	To study the effect of mechanical stretching, BMDMs or RAW264.7 cells were treated with Yoda1, with or without pretreatment with inhibitors for 30 min. The inhibitors included cyclosporin A, FK506, echinomycin, GsMTx4, BAPTA-AM and RN-1734. In some experiments, BMDMs were treated with GSK1016790A. (Data sourced from selleck products)

References
[1] Radhakrishnan Gnanasambandam, et al. Biophys J. 2017 Jan 10;112(1):31-45. [2] Zhang X, et al. Theranostics. 2022 Jan 16;12(4):1621-1638. [3] Liu Z, et al. Biochem Biophys Res Commun. 2022 Nov 21;638:140-146. [4] Xu H, et al. FASEB J. 2022 Aug;36(8):e22423. + Expand

References

[4] Xu H, et al. FASEB J. 2022 Aug;36(8):e22423.

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Product Information

Molecular Formula:	C₁₈₅H₂₇₃N₄₉O₄₅S₆
Molecular Weight:	4095.86
CAS No.:	1209500-46-8
Appearance:	White Powder
Peptide Content (by %N):	≥80%
Single Impurity (by HPLC):	≤1.0%
Assay (By Anhydrous, Acetic Acid-free):	95.0~105.0%
MS:	Consistent
Packing:	plastic vial(dedicated for peptide packing) or glass vial, quantity according to customer's detail requirement.
Storage (From the date of receipt):	3 years-20°C powder
Shipping:	Room temperature shipping(Stability testing shows this product can be shipped without any cooling measures.)