Name: Dacinostat (LAQ824)
Brand: Selleck Chemicals
SKU: S1095
Rating: 5 (18 reviews)

CAS No. 404951-53-7

Selleck's Dacinostat (LAQ824) has been cited by 18 publications

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HDAC Signaling Pathway Map

Biological Activity

Description

Dacinostat (LAQ824, NVP-LAQ824) is a novel HDAC inhibitor with IC50 of 32 nM and is known to activate the p21 promoter.

Targets

HDAC ^[1]
(Cell-free assay)

32 nM

In vitro

LAQ824 activates the expression of the gene encoding the p21 cell cycle inhibitor by activating the p21 promoter with 50% of the maximal promoter activation (AC50) of 0.30 μM. LAQ824 inhibits the cell growth of both H1299, a non-small cell lung carcinoma line, and HCT116, a colon cancer cell line with IC50 of 0.15 μM and 0.01 μM, respectively, and the antiproliferative effect of LAQ824 is selective toward the tumor cell lines while inducing only growth arrest in normal fibroblasts. Furthermore, LAQ824 induces a dose-dependent increase of p21 protein in A549 cells and an increase in the hypophosphorylated state of the Rb tumor suppressor. ^[1] A recent study shows that LAQ824 induces chromatin changes at the level of the IL-10 gene promoter that lead to enhanced recruitment of the transcriptional repressors HDAC11 and PU.1 and inhibits IL-10 production in BALB/c murine macrophages. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

H1299	NH;Od\|ZIem:5dHigbY5pcWKrdHnvckBie3OjeR?=			M3fjbGlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJOTJ7OTDoeY1idiCudX7nJINiemOrbn;tZUBk\WyuIHfyc5d1cCxiSVO1NF0xNjF3IN88US=>	MY[xOFUzOTR{Mh?=
HCT116	NV;ySnI2T3Kxd4ToJIlvcGmkaYTpc44h[XO\|YYm=			M4DZVGlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJS1RzMU[gbJVu[W5iY3;sc44h[2WubDDndo94fGhuIFnDOVA:OC5yMTFOwG0>	M4X2T\|E1PTJzNEKy
HCT116	Mlu5R5l1d3SxeHnjbZR6KGG\|c3H5			NEjOO5ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJS1RzMU[gZ4VtdHNiYomgUXRVKGG\|c3H5MEBKSzVyPUCuNFUh\|ryP	NXLoO4RqOTd7NEG2NlU>
human mammary epithelial cells	MlvyR5l1d3SxeHnjbZR6KGG\|c3H5			M1TkRmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJI1idW2jcomg[ZBqfGinbHnhcEBk\WyuczDifUBOXFRiYYPzZZktKEmFNUC9NUDPxE1?	NF;JPYoyPzl2MU[yOS=>
HEK293 cells	NGDucnFHfW6ldHnvckBie3OjeR?=			NWnXbmE6UW6qaXLpeIlwdiCxZjDISGFEOSCrbjDISWszQTNiY3XscJMtKEmFNUC9NE4xODJ4IN88US=>	MVKxPFMxQDV4Mx?=
HeLa cells	M4fr[WZ2dmO2aX;uJIF{e2G7	NEjYSWkxNjNizszN	MmTFNVYhcA>?	M4noemlvcGmkaYTpc44hd2ZiSFTBR\|QudWWmaXH0[YQhemWycnXzd4lwdiCxZjDNSWYzNWSncHXu[IVvfCC2cnHud4NzcXC2aX;uJIlvKEinTHGgZ4VtdHNiYYSgNE4{KHWPIHHmeIVzKDF4IHjyd{BjgSCudXPp[oVz[XOnIILldI9zfGW{IHflcoUh[XO\|YYm=	M1i1dlE4QTV4OUi4
HCT116 cells	M4rueXBzd2yrZnXyZZRqd25iYYPzZZk>			NVW4c\|JuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[nliTWTTJIF{e2G7LDDJR\|UxRTBwMEGzJO69VQ>?	NEf3UYIzODJyNUO5OC=>
H1299 cells	MUfQdo9tcW[ncnH0bY9vKGG\|c3H5			MnfxRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKMUK5PUBk\WyuczDifUBOXFNiYYPzZZktKEmFNUC9NE4yPjFizszN	MmXkNlAzODV\|OUS=
H460 cells	MlLMS5Jwf3SqIHnubIljcXSrb36gZZN{[Xl?		MWG0PEBp	Ml\0S5Jwf3SqIHnubIljcXSrb36gc4YhcHWvYX6gTFQ3OCClZXzsd{Bi\nSncjC0PEBpenNiYomgV3JDKGG\|c3H5MEBKSzVyPUCuNFch\|ryP	NIPKeVMzOTB5M{G2NC=>
HCT116 cells	MXXHdo94fGhiaX7obYJqfGmxbjDhd5NigQ>?		NXLnfIQ1PDhiaB?=	NInZO4xIem:5dHigbY5pcWKrdHnvckBw\iCqdX3hckBJS1RzMU[gZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KFOUQjDhd5NigSxiSVO1NF0xNjBzODFOwG0>	MmfhNlExPzNzNkC=
CCRF-CEM cells	NFSyWo1Rem:uaX\ldoF1cW:wIHHzd4F6			MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEOFUl[tR2VOKGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjJ3MTFOwG0>	M3LJcVIyPjJzOEiz
K562 cells	M4XkSnBzd2yrZnXyZZRqd25iYYPzZZk>			MmLoRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCNNU[yJINmdGy\|IHL5JHNTSiCjc4PhfUwhT0l3ME2wMlU2KM7:TR?=	MlrtNlE3OjF6OEO=
MOLT4 cells	MXjQdo9tcW[ncnH0bY9vKGG\|c3H5			MkLvRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPT1zUOEBk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9NE4{OTFizszN	MmrUNlE3OjF6OEO=
SR cells	NWrUcGZWWHKxbHnm[ZJifGmxbjDhd5NigQ>?			NWjTbHF5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTVkBk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9Nk46QSEQvF2=	NFXwXmkzOTZ{MUi4Ny=>
A549 cells	MnO2VJJwdGmoZYLheIlwdiCjc4PhfS=>			M2\0W2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUW0PUBk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9NU4xQSEQvF2=	M4jIcVIyPjJzOEiz
EKVX cells	NGHWZWRRem:uaX\ldoF1cW:wIHHzd4F6			NHHoSoFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFXLWngh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVEvPzVizszN	MWiyNVYzOTh6Mx?=
HOP62 cells	M1HYUnBzd2yrZnXyZZRqd25iYYPzZZk>			NGjw[mNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjPVFYzKGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjVyMjFOwG0>	M3TPOVIyPjJzOEiz
HOP92 cells	NVPJZnVJWHKxbHnm[ZJifGmxbjDhd5NigQ>?			M{nuOWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSF;QPVIh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvOTl7IN88US=>	NGnZSFAzOTZ{MUi4Ny=>
NCI-H226 cells	M1LO[3Bzd2yrZnXyZZRqd25iYYPzZZk>			MmO3RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCQQ1mtTFIzPiClZXzsd{BjgSCVUlKgZZN{[XluIFfJOVA:OS52MTFOwG0>	M{\D[\|IyPjJzOEiz
NCI-H23 cells	NUTSXXZ5WHKxbHnm[ZJifGmxbjDhd5NigQ>?			NX36dVd{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDJ\|IHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD1zLkWg{txO	NGD4co0zOTZ{MUi4Ny=>
NCI-H322M cells	NUn3d3gxWHKxbHnm[ZJifGmxbjDhd5NigQ>?			MlzwRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCQQ1mtTFMzOk1iY3XscJMh[nliU2LCJIF{e2G7LDDHTVUxRTJwMk[g{txO	MleyNlE3OjF6OEO=
NCI-H460 cells	NE\re4JRem:uaX\ldoF1cW:wIHHzd4F6			Ml7xRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCQQ1mtTFQ3OCClZXzsd{BjgSCVUlKgZZN{[XluIFfJOVA:OC55IN88US=>	Mn3sNlE3OjF6OEO=
COLO205 cells	NIjTV5lRem:uaX\ldoF1cW:wIHHzd4F6			NVK2XnBTSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDDU2xQOjB3IHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD1zLkC4JO69VQ>?	M{C5SFIyPjJzOEiz
HCT116 cells	M4nTe3Bzd2yrZnXyZZRqd25iYYPzZZk>			MV3BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9NE4{PDdizszN	MVKyNVYzOTh6Mx?=
HCT15 cells	NGDvepFRem:uaX\ldoF1cW:wIHHzd4F6			MYDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEG1JINmdGy\|IHL5JHNTSiCjc4PhfUwhT0l3ME2yMlA4KM7:TR?=	M{Lt[VIyPjJzOEiz
HT-29 cells	MVTQdo9tcW[ncnH0bY9vKGG\|c3H5			NVXXbFQ4SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIWE0zQSClZXzsd{BjgSCVUlKgZZN{[XluIFfJOVA:OC53Nk[g{txO	NEW5SmQzOTZ{MUi4Ny=>
KM12 cells	M3nBR3Bzd2yrZnXyZZRqd25iYYPzZZk>			NWnR[GN1SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDLUVEzKGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0yNjB2IN88US=>	MWmyNVYzOTh6Mx?=
SW620 cells	MXjQdo9tcW[ncnH0bY9vKGG\|c3H5			M4fyVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU2e2NlAh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvPDd6IN88US=>	NYLM[4xGOjF4MkG4PFM>
SF268 cells	MkmxVJJwdGmoZYLheIlwdiCjc4PhfS=>			M4KxZmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1[yOlgh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVEvPTNizszN	M1zSeFIyPjJzOEiz
SF295 cells	NHP3NY5Rem:uaX\ldoF1cW:wIHHzd4F6			M3vrRWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1[yPVUh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvPzR\|IN88US=>	NETI[GUzOTZ{MUi4Ny=>
SF539 cells	NITzWnNRem:uaX\ldoF1cW:wIHHzd4F6			M1\qb2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU1[1N\|kh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvQTZ3IN88US=>	NHm0S3kzOTZ{MUi4Ny=>
SNB19 cells	NW[0eVRMWHKxbHnm[ZJifGmxbjDhd5NigQ>?			NI\ISYpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPORlE6KGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0yNjV\|IN88US=>	NWLHW2p2OjF4MkG4PFM>
SNB75 cells	NVP4cJBYWHKxbHnm[ZJifGmxbjDhd5NigQ>?			M4jCWGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU17CO\|Uh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvOzF6IN88US=>	MnX4NlE3OjF6OEO=
U251 cells	NEPO[HlRem:uaX\ldoF1cW:wIHHzd4F6			NYPnW\|I{SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDVNlUyKGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjd5MzFOwG0>	MXGyNVYzOTh6Mx?=
LOXIMVI cells	MXPQdo9tcW[ncnH0bY9vKGG\|c3H5			NGr2VW5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFzPXGlOXkliY3XscJMh[nliU2LCJIF{e2G7LDDHTVUxRTFwMEig{txO	MX[yNVYzOTh6Mx?=
MALME-3M cells	NH7we45Rem:uaX\ldoF1cW:wIHHzd4F6			NHfoV2VCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3BUG1GNTOPIHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD1yLkS2NUDPxE1?	NYW0SY9oOjF4MkG4PFM>
M14 cells	Ml;SVJJwdGmoZYLheIlwdiCjc4PhfS=>			Mn7CRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPMUSgZ4VtdHNiYomgV3JDKGG\|c3H5MEBIUTVyPUCuPFQ6KM7:TR?=	NWjPRlhwOjF4MkG4PFM>
MDA-MB-435 cells	M1uwVnBzd2yrZnXyZZRqd25iYYPzZZk>			NXPPNWRqSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDNSGEuVUJvNEO1JINmdGy\|IHL5JHNTSiCjc4PhfUwhT0l3ME2wMlU{PyEQvF2=	M2e5WlIyPjJzOEiz
SK-MEL-28 cells	NFrwXJNRem:uaX\ldoF1cW:wIHHzd4F6			NHO0eGtCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLMW1GVC1{ODDj[YxteyCkeTDTVmIh[XO\|YYmsJGdKPTB;MD65PVMh\|ryP	NUL2W\|JPOjF4MkG4PFM>
SK-MEL-5 cells	MWnQdo9tcW[ncnH0bY9vKGG\|c3H5			NYrD[npESW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvNTDj[YxteyCkeTDTVmIh[XO\|YYmsJGdKPTB;MD6yO\|Eh\|ryP	NVnSeHlNOjF4MkG4PFM>
UACC257 cells	M4LTSnBzd2yrZnXyZZRqd25iYYPzZZk>			NIDpPYlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGXBR2MzPTdiY3XscJMh[nliU2LCJIF{e2G7LDDHTVUxRTBwM{mzJO69VQ>?	MXiyNVYzOTh6Mx?=
UACC62 cells	MWLQdo9tcW[ncnH0bY9vKGG\|c3H5			MmnMRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCXQVPDOlIh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvOzd7IN88US=>	NXzvZ5lLOjF4MkG4PFM>
IGROV1 cells	NHXmTlVRem:uaX\ldoF1cW:wIHHzd4F6			M1;1T2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iSVfSU3YyKGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjF6NzFOwG0>	NIPIeFczOTZ{MUi4Ny=>
OVCAR3 cells	M1;D[HBzd2yrZnXyZZRqd25iYYPzZZk>			NHfnTVlCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF;WR2FTOyClZXzsd{BjgSCVUlKgZZN{[XluIFfJOVA:OS5zMTFOwG0>	NYOwcGt7OjF4MkG4PFM>
OVCAR4 cells	MmLTVJJwdGmoZYLheIlwdiCjc4PhfS=>			M3jWcWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iT2\DRXI1KGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0{NjJzIN88US=>	NWDqRWoyOjF4MkG4PFM>
OVCAR5 cells	NV\DfZcyWHKxbHnm[ZJifGmxbjDhd5NigQ>?			M{nrWGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iT2\DRXI2KGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjJ4IN88US=>	MnvKNlE3OjF6OEO=
OVCAR8 cells	NHi1RXlRem:uaX\ldoF1cW:wIHHzd4F6			NUnudGVISW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDPWmNCWjhiY3XscJMh[nliU2LCJIF{e2G7LDDHTVUxRTBwNEWg{txO	NGKxc44zOTZ{MUi4Ny=>
NCI/ADR-RES cells	NIXCPI1Rem:uaX\ldoF1cW:wIHHzd4F6			MVnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE6FST;BSHIuWkWVIHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD1yLkG4JO69VQ>?	NHnnVWEzOTZ{MUi4Ny=>
SKOV3 cells	NYXuNnJGWHKxbHnm[ZJifGmxbjDhd5NigQ>?			NGLuclNCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGPLU3Y{KGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0xNjV{NDFOwG0>	NHnsZWIzOTZ{MUi4Ny=>
786-0 cells	MkfFVJJwdGmoZYLheIlwdiCjc4PhfS=>			M4S3cmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iN{i2MVAh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVIvOzhizszN	Mm\6NlE3OjF6OEO=
A498 cells	MUPQdo9tcW[ncnH0bY9vKGG\|c3H5			M4rObWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQUS5PEBk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9NU4yOSEQvF2=	NXHnc3pKOjF4MkG4PFM>
ACHN cells	MWnQdo9tcW[ncnH0bY9vKGG\|c3H5			MnnyRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCDQ1jOJINmdGy\|IHL5JHNTSiCjc4PhfUwhT0l3ME2xMlEh\|ryP	NFnKTG8zOTZ{MUi4Ny=>
Caki1 cells	NWj0OWFFWHKxbHnm[ZJifGmxbjDhd5NigQ>?			M1zpemFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQ3HrbVEh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvPjF7IN88US=>	MoLPNlE3OjF6OEO=
SN12C cells	M2\QeXBzd2yrZnXyZZRqd25iYYPzZZk>			M2DFXGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iU16xNmMh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVMvQTJizszN	MmPDNlE3OjF6OEO=
TK10 cells	MlfmVJJwdGmoZYLheIlwdiCjc4PhfS=>			MYLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFSNMUCgZ4VtdHNiYomgV3JDKGG\|c3H5MEBIUTVyPUCuOFQ1KM7:TR?=	M3KzZ\|IyPjJzOEiz
UO31 cells	NE\4[5VRem:uaX\ldoF1cW:wIHHzd4F6			MYHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKFWRM{GgZ4VtdHNiYomgV3JDKGG\|c3H5MEBIUTVyPUCuOFUzKM7:TR?=	NYrreXdyOjF4MkG4PFM>
PC3 cells	M3;4WHBzd2yrZnXyZZRqd25iYYPzZZk>			NGO2c3JCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIGDDN{Bk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9NlAh\|ryP	NFzZdoszOTZ{MUi4Ny=>
DU145 cells	MVXQdo9tcW[ncnH0bY9vKGG\|c3H5			M3;rR2FvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iRGWxOFUh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVI6NjlizszN	MmHWNlE3OjF6OEO=
MCF7 cells	NV7ET3F3WHKxbHnm[ZJifGmxbjDhd5NigQ>?			M2K4XWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iTVPGO{Bk\WyuczDifUBUWkJiYYPzZZktKEeLNUC9Ok46OSEQvF2=	MVKyNVYzOTh6Mx?=
MDA-MB-231 cells	NIfROI5Rem:uaX\ldoF1cW:wIHHzd4F6			MlvRRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPRFGtUWIuOjNzIHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD1\|MT63JO69VQ>?	NVnSVXBSOjF4MkG4PFM>
Hs 578T cells	MX;Qdo9tcW[ncnH0bY9vKGG\|c3H5			NX[4S3ZOSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDId{A2PziWIHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD13LkiyJO69VQ>?	NF2xNZMzOTZ{MUi4Ny=>
BT549 cells	MlnZVJJwdGmoZYLheIlwdiCjc4PhfS=>			M1rwdmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iQmS1OFkh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVYvODRizszN	M3LyS\|IyPjJzOEiz
T47D cells	NUnWSFJYWHKxbHnm[ZJifGmxbjDhd5NigQ>?			NV7qb\|RoSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDUOFdFKGOnbHzzJIJ6KFOUQjDhd5NigSxiR1m1NF0zQC56IN88US=>	M{DRV\|IyPjJzOEiz
MDA-MB-468 cells	MmPkVJJwdGmoZYLheIlwdiCjc4PhfS=>			MmLVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCPRFGtUWIuPDZ6IHPlcIx{KGK7IGPSRkBie3OjeTygS2k2OD13Lki4JO69VQ>?	NUjte\|NqOjF4MkG4PFM>
NCI-H226 cells	NIjMb2FEgXSxdH;4bYNqfHliYYPzZZk>			MnzNR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gUmNKNUh{Mk[gZ4VtdHNuIFzDOVA:OjBizszN	NXvETZNkOjF4MkG4PFM>
KM12 cells	M2\sZ2N6fG:2b4jpZ4l1gSCjc4PhfS=>			MmLoR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gT20yOiClZXzsd{whVEN3ME2yPU46KM7:TR?=	Mn;2NlE3OjF6OEO=
SF295 cells	M3LBVWN6fG:2b4jpZ4l1gSCjc4PhfS=>			MlqwR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2YzQTViY3XscJMtKEyFNUC9Ok46OSEQvF2=	MYqyNVYzOTh6Mx?=
SNB19 cells	MlHIR5l1d3SxeHnjbZR6KGG\|c3H5			M2XGb2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNPSjF7IHPlcIx{NCCOQ{WwQVMyNjdizszN	MV[yNVYzOTh6Mx?=
U251 cells	MVTDfZRwfG:6aXPpeJkh[XO\|YYm=			NYn2[2tFS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hXTJ3MTDj[YxteyxiTFO1NF02Njh{IN88US=>	MnjzNlE3OjF6OEO=
LOXIMVI cells	MV;DfZRwfG:6aXPpeJkh[XO\|YYm=			M2T2fGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGxQYEmPVlmgZ4VtdHNuIFzDOVA:Pi5yNDFOwG0>	NV;1e4djOjF4MkG4PFM>
MDA-MB-435 cells	MWDDfZRwfG:6aXPpeJkh[XO\|YYm=			NVrubIVtS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVUSDLV3CMVQ{PSClZXzsd{whVEN3ME2yPE45KM7:TR?=	M2T1TVIyPjJzOEiz
SK-MEL-28 cells	NUD5[VhES3m2b4TvfIlkcXS7IHHzd4F6			MWHDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvMkigZ4VtdHNuIFzDOVA:OzFwOTFOwG0>	MYSyNVYzOTh6Mx?=
SK-MEL-5 cells	NF6yOW5EgXSxdH;4bYNqfHliYYPzZZk>			MYnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT{1OTUxvNTDj[YxteyxiTFO1NF01NjB{IN88US=>	NXTVVINTOjF4MkG4PFM>
UACC257 cells	M{XTZWN6fG:2b4jpZ4l1gSCjc4PhfS=>			MlzvR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gWWFESzJ3NzDj[YxteyxiTFO1NF01PC56IN88US=>	MnGyNlE3OjF6OEO=
UACC62 cells	NETpZVVEgXSxdH;4bYNqfHliYYPzZZk>			M1OxcGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHVCS0N4MjDj[YxteyxiTFO1NF03NjN7IN88US=>	NXK2[VZkOjF4MkG4PFM>
IGROV1 cells	Mm[wR5l1d3SxeHnjbZR6KGG\|c3H5			NHnwenFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBKT1KRVkGgZ4VtdHNuIFzDOVA:PDhwOTFOwG0>	NUn4fWZNOjF4MkG4PFM>
OVCAR5 cells	MXfDfZRwfG:6aXPpeJkh[XO\|YYm=			NH\hWm1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBQXkODUkWgZ4VtdHNuIFzDOVA:OzhwOTFOwG0>	M1;Q[\|IyPjJzOEiz
SKOV3 cells	MYPDfZRwfG:6aXPpeJkh[XO\|YYm=			M4XtSGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMV1Z\|IHPlcIx{NCCOQ{WwQVI5NjVizszN	NYX0[YxmOjF4MkG4PFM>
A498 cells	M2naZWN6fG:2b4jpZ4l1gSCjc4PhfS=>			NXjw[o1PS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTR7ODDj[YxteyxiTFO1NF02Njh6IN88US=>	M{n0dlIyPjJzOEiz
Caki1 cells	MoW3R5l1d3SxeHnjbZR6KGG\|c3H5			NHPiWJFEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBE[WurMTDj[YxteyxiTFO1NF02Njd7IN88US=>	MmPXNlE3OjF6OEO=
HCT116 cells	NVnnPZNjWHKxbHnm[ZJifGmxbjDhd5NigQ>?			NHHyPIFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjDWFEyPiClZXzsd{whUUN3ME2wMlAyQSEQvF2=	MoPFNlE4PDJ2OU[=
HCT116 cells	NX\hU4pKWHKxbHnm[ZJifGmxbjDhd5NigQ>?			NVryTIQ5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[XO\|ZYPz[YQh[XNiZ4Lve5RpKGmwaHnibZRqd25uIFnDOVA:OC5yMUmg{txO	MUGyNVY2ODJ{MR?=
H1299 cells	M1PtW3Bzd2yrZnXyZZRqd25iYYPzZZk>			MnTlRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCKMUK5PUBk\WyuczygTWM2OD1yLkGg{txO	MmfwNlE3PTB{MkG=
HCT116 cells	NX;SZYxRWHKxbHnm[ZJifGmxbjDhd5NigQ>?			MoHMTY5pcWKrdH;yfUBkd26lZX70doF1cW:wIHHnZYlve3RiSFPUNVE3KGOnbHzzJJBzd2yrZnXyZZRqd25uIFnDOVA:OC5yMTFOwG0>	NUnHXmdPOTR4MUOzNVI>
RPMI8226 cells	NFX5bpFRem:uaX\ldoF1cW:wIHHzd4F6			M{LOeGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iUmDNTVgzOjZiY3XscJMh[nliU2LCJIF{e2G7LDDHTVUxRTBwNUKzJO69VQ>?	MlzqNlE3OjF6OEO=
CHO cells	MlrLSpVv[3Srb36gZZN{[Xl?			NHPCR5VKdmirYnn0bY9vKG:oIHj1cYFvKEWURzDlfJBz\XO\|ZXSgbY4hS0iRIHPlcIx{KGK7IHH1eI9u[XSnZDDwZZRkcCClbHHtdEBmdGWldILvdIh6e2mxbH;nfUBie3OjeTygTWM2OD1zMj6yJO69VQ>?	NF\FN5YzODJyNUO5OC=>
HL-60(TB) cells	NILGWZZRem:uaX\ldoF1cW:wIHHzd4F6			NIfnbpBCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIFjMMVYxMFSEKTDj[YxteyCkeTDTVmIh[XO\|YYmsJGdKPTB;MD6yOFkh\|ryP	NXPyZZl{OjF4MkG4PFM>
RXF393 cells	NWjnToMzWHKxbHnm[ZJifGmxbjDhd5NigQ>?			MlrGRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6\|dDDoeY1idiCUWF[zPVMh[2WubIOgZpkhW1KEIHHzd4F6NCCJSUWwQVAvODFizszN	MWiyNVYzOTh6Mx?=
RXF393 cells	MVzDfZRwfG:6aXPpeJkh[XO\|YYm=			NXfNWo85S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hWliIM{mzJINmdGy\|LDDMR\|UxRTF3LkOg{txO	M2XBbVIyPjJzOEiz

Click to View More Cell Line Experimental Data

In vivo

In HCT116 and human colon tumor xenografts in nude mice, LAQ824 treatment at 100 mg/kg produces the inhibitory effects on tumor growth in a dose-dependent mode without general cytotoxicity. ^[1]

Protocol (from reference)

Kinase Assay:^[1]	In Vitro Histone Deacetylase Assay: HDAC enzymes are partially purified from H1299 cell lysate by ion exchange chromatography using the Q Sepharose Fast Flow column. Enzyme complexes are collected from 500 mg of total cell lysate by immunoprecipitation with cdk2 polyclonal antibody or cdk1/cdc2 monoclonal antibody. Immunoprecipitates are resuspended in kinase buffer (50 mM Hepes, pH 8, 10 mM MgCl₂, 2.5 mM EDTA, 1 mM dithiothreitol, 20 mM ATP, 10 mM β-glycerophosphate, 0.1 mM NaVO₄, 1 mM sodium fluoride, 50 mM ATP, 10 μCi of [γ-³²P]ATP) along with 1 μg of pRb recombinant protein substrate (cdk2) or 10 mL of H1 histone mixture containing 20 μg of substrate (cdc2). Phosphorylated Rb and H1 histone are resolved by electrophoresis and quantitated using a PhosphorImager.
Cell Research:^[1]	Cell lines: H1299, HCT116, DU145, PC3 and MDA435 cells Concentrations: 0-10 μM Incubation Time: 48 hours Method: Cell proliferation is measured using an adaptation of published procedures (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfonyl)-2H-tetrazolium assay). The cells are seeded in 12-well dishes and cultured in RPMI 1640 containing 10% FBS. The cells are cultured in the presence of various concentrations of TSA (up to 1,000 ng/mL). To examine the growth inhibition by TSA, viable cell numbers are determined by trypan blue dye exclusion, counted in a Nesbauer-type hemocytometer for 0 hour, 24 hours, and 48 hours. The same amount of ethanol is added to the RPMI 1640 medium as the control experiment. All experiments are performed in duplicate and repeated 3 times The average background value (treatment with medium alone) is subtracted from each experimental well; triplicate values are averaged for each compound dilution. The following formulas are used to calculate the percentage of growth: If X0, %Growth=(X-T₀)/T₀100; If X>T₀, %Growth=(X-T₀)/(GC-T₀)100. where T₀ is the average value of T₀ − background, GC is the average value of untreated cells (in triplicate) − background, and X is the average value of compound-treated cells (in triplicate)-background. The “% Growth” is plotted against compound concentration and used to calculate the IC50 using the linear regression techniques between data points to predict the concentration of compounds at 50% inhibition. (Only for Reference)
Animal Research:^[1]	Animal Models: HCT116 cells is injected s.c. into the right axillary (lateral) region of outbred athymic (nu/nu) female mice. Dosages: ≤100 mg/kg Administration: Administered via i.v. (Only for Reference)

References

Solubility (25°C)

In vitro	DMSO	76 mg/mL (200.28 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 5%DMSO+40%PEG300+5%Tween80+50%ddH2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	15 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight	379.459
Formula	C₂₂H₂₅N₃O₃
CAS No.	404951-53-7
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	C1=CC=C2C(=C1)C(=CN2)CCN(CCO)CC3=CC=C(C=C3)C=CC(=O)NO

Download Dacinostat (LAQ824) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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