Sodium butyrate

Catalog No.S1999 Synonyms: NaB, Butanoic acid sodium salt

For research use only.

Sodium butyrate (NaB, Butanoic acid sodium salt), sodium salt of butyric acid, is a histone deacetylase inhibitor and competitively binds to the zinc sites of class I and II histone deacetylases (HDACs). Sodium butyrate (NaB) inhibits cell cycle progression, promotes differentiation, and induces apoptosis and autophagy in several types of cancer cells.

Sodium butyrate Chemical Structure

CAS No. 156-54-7

Selleck's Sodium butyrate has been cited by 19 publications

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Biological Activity

Description Sodium butyrate (NaB, Butanoic acid sodium salt), sodium salt of butyric acid, is a histone deacetylase inhibitor and competitively binds to the zinc sites of class I and II histone deacetylases (HDACs). Sodium butyrate (NaB) inhibits cell cycle progression, promotes differentiation, and induces apoptosis and autophagy in several types of cancer cells.
Targets
Histone deacetylase [6]
In vitro

Butyrate mediates growth inhibition of colonic cancer cells and thereby to elucidate the molecular link between a high-fiber diet and the arrest of colon carcinogenesis. Butyrate induces p21 mRNA expression in an immediate-early fashion, through transactivation of a promoter cis-element(s) located within 1.4 kb of the transcriptional start site, independent of p53 binding. [1] Sodium butyrate, at physiological concentrations, induces apoptosis in 2 adenoma cell lines, RG/C2 and AA/Cl, and in the carcinoma cell line PC/JW/FI. [2] Butyrate exerts potent effects on a variety of colonic mucosal functions such as inhibition of inflammation and carcinogenesis, reinforcing various components of the colonic defence barrier and decreasing oxidative stress. [3] Butyrate inhibits most HDAC except class III HDAC and class II HDAC6 and -10. Butyrate also is the most effective fatty acid in stimulating or repressing the expression of specific genes. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human K562 cells NVXnbHh[WHKxbHnm[ZJifGmxbjDhd5NigQ>? M3TCblEhdU1? MljQRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCNNU[yJINmdGy|IHH0JFEhdU1iaX6gdJJme2WwY3Wgc4Yh\3Wjbn;zbY5m NFP1R5IzOzl|N{m3PS=>
human H1299 cells MUDGeY5kfGmxbjDhd5NigQ>? MYi3JIRigXN? Moq3TY5pcWKrdHnvckBw\iCqdX3hckBWW1BzL2XBSlEhcW5iaIXtZY4hUDF{OUmgZ4VtdHNiYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJIlvKGOxbH;ufUBnd3KvYYTpc44h[W[2ZYKgO{Bl[Xm|LDDFR|UxRTNizszN NFz6dZkzPTJ{OU[0Ny=>
human JEG3 cells MnXQSpVv[3Srb36gZZN{[Xl? M1ntbmlvcGmkaYTpc44hd2ZiYYLvcYF1[XOnIHnuJIh2dWGwIFrFS|Mh[2WubIOgZpkhe2OrboTpcIxifGmxbjDzdIVkfHKxbXX0dpktKEmFNUC9NE4xODF3IN88US=> M1PPZVIxOTR6NU[0
Assay
Methods Test Index PMID
Western blot Cyclin D3 / CDK4 / CDK6 / CDK2 ; p15INK4b / p21Cip1 ; Phospho-H3 Ser10 / Acetyl-H3Lys9 / Acetyl-H3Lys14 / Dimethyl-H3 24280698
Immunofluorescence Acetyl-H3Lys9 24280698
In vivo Sodium butyrate significantly extends survival in a dose-dependent manner, improves body weight and motor performance, and delays the neuropathological sequelae in the R6/2 transgenic mouse model of Huntington's disease (HD). Sodium butyrate also increases histone and specificity protein-1 acetylation and protects against 3-nitropropionic acid neurotoxicity. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 110.09
Formula

C4H7NaO2

CAS No. 156-54-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCCC(=O)[O-].[Na+]

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT00800930 Completed Biological: Sodium Butyrate|Biological: Saline Shigellosis International Centre for Diarrhoeal Disease Research Bangladesh|Swedish International Development Cooperation Agency (SIDA)|Karolinska Institutet January 2005 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-08-01)

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