Catalog No.S2239

Tubacin Chemical Structure

Molecular Weight(MW): 721.86

Tubacin is a highly potent and selective, reversible, cell-permeable HDAC6 inhibitor with an IC50 of 4 nM in a cell-free assay, approximately 350-fold selectivity over HDAC1.

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Cited by 8 Publications

3 Customer Reviews

  • Verification of Hdac6 deletion in knockout MEFs. Expression of HDAC6 and acetylation of tubulin were analyzed by immunoblotting.

    Nat Biotechnol, 2015, 33(4): 415-23 . Tubacin purchased from Selleck.

    Inhibition of HDAC6 by tubacin induced the acetylation and membrane translocation of wild-type PTEN, but not K163R mutant. U-87 MG or NCl-H1650 cells were transfected with EGFP-PTEN or EGFP-PTEN-K163R for 24 h and then treated with tubacin for 24 h. *P<0.01.

    Oncogene, 2016, 35(18):2333-44. Tubacin purchased from Selleck.

  • A, immunoblotting analysis of E-cadherin, ZO-1, Vimentin, N-cadherin, acetyl-α-tubulin, and α-tubulin in MCF-10A cells. Cells were treated with SB431542 (5 μM), Tubacin (5 μM), Paclitaxol (PTX) (5 nM), Nocodazol (NDL) (5 nM), TSA (50 ng/liter), or Nicotiamide (Nico) (10 mM), followed by co-incubation with TGF-β (2 ng/ml).

    J Biol Chem, 2016, 291(10):5396-405. Tubacin purchased from Selleck.

Purity & Quality Control

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Biological Activity

Description Tubacin is a highly potent and selective, reversible, cell-permeable HDAC6 inhibitor with an IC50 of 4 nM in a cell-free assay, approximately 350-fold selectivity over HDAC1.
Features The first known selective inhibitor of α-tubulin deacetylation.
HDAC6 [1]
(Cell-free assay)
4 nM
In vitro

Tubacin, without directly stabilizing microtubules, induces an increase in α-tubulin acetylation with EC50 of 2.5 μM in A549 cells. Tubacin inhibits HDAC6-mediated α-tubulin deacetylation, and inhibits the migration of both wild-type and HDAC6-overexpressing cells. [2] Tubacin, in combination with paclitaxel, synergistically enhances tubulin acetylation. [3] Tubacin significantly inhibits both drug-sensitive and drug–resistant MM cell growth with IC50 of 5–20 μM, and induces cell apoptosis by activation of caspases. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HeLa cells MoPYSpVv[3Srb36gZZN{[Xl? NGXDcFQ3KGh? M2PKUWlvcGmkaYTpc44hd2ZiSFTBR|YhcW5iaIXtZY4hUGWOYTDj[YxteyCjc4Pld5Nm\CCjczDy[YR2[3Srb36gbY4hUzRyIHj5dIVz[WOndInsZZRqd25ib3[gZYxxcGFvdIXieYxqdiCrbnP1ZoF1\WRiZn;yJFYhcHK|IHL5JIludXWwb3\seY9z\XOlZX7j[UBie3OjeTygTWM2OD1{Lkmg{txO NIDZO3IzPTR3NEK3NC=>
human A549 cells M{LXPGZ2dmO2aX;uJIF{e2G7 MX\Jcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFteGijLYT1ZpVtcW5iYXPleJlt[XSrb36gZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcImsJGVEPTB;Mj65JO69VQ>? NFLPUVQyPjRyOECwNy=>
human T24 cells MUTGeY5kfGmxbjDhd5NigQ>? M{TLbmlv\HWldHnvckBw\iCqaYP0c45mKEh|IHHj[ZR6dGG2aX;uJIlvKGi3bXHuJHQzPCClZXzsd{whTUN3ME2yMlkh|ryP NUX2ZVBtOTlzMUG0OlY>

... Click to View More Cell Line Experimental Data

In vivo In chick embryos, inhibition of HDAC6 activity by Tubacin reduces the formation of new blood vessels in matrigel/nylon mesh. In angioreactors implanted in mice, Tubacin also impairs the formation of new blood vessels. [5]


Kinase Assay:


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Enzyme Inhibition Assay:

Enzyme inhibition assays are performed using the Reaction Biology HDAC Spectrum platform. The HDAC1, 2, 4, 5, 6, 7, 8, 9, 10, and 11 assays used isolated recombinant human protein; HDAC3/NcoR2 complex is used for the HDAC3 assay. Substrate for HDAC1, 2, 3, 6, 10, and 11 assays is a fluorogenic peptide from p53 residues 379-382 (RHKKAc); substrate for HDAC8 is fluorogenic diacyl peptide based on residues 379-382 of p53 (RHKAcKAc). Acetyl-Lys(trifluoroacetyl)-AMC substrate is used for HDAC4, 5, 7, and 9 assays. Compounds are dissolved in DMSO and tested in 10-dose IC50 mode with 3-fold serial dilution starting at 30 μM. Control Compound Trichostatin A (TSA) is tested in a 10-dose IC50 with 3-fold serial dilution starting at 5 μM. IC50 values are extracted by curve-fitting the dose/response slopes.
Cell Research:


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  • Cell lines: Drug-sensitive (MM.1S, U266, INA-6, and RPMI8226) and drug-resistant (RPMI-LR5 and RPMI-Dox40) MM cell lines
  • Concentrations: ~20 μM
  • Incubation Time: 72 hours
  • Method:

    The inhibitory effect of bortezomib and/or tubacin on MM cell growth is assessed by measuring 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye absorbance. All experiments are performed in quadruplicate.

    (Only for Reference)
Animal Research:


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  • Animal Models: Athymic nude mice implanted with angioreactors
  • Formulation: DMSO
  • Dosages: --
  • Administration: Tubacin is filled in semiclosed angioreactors, and then implanted into the mice.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (138.53 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 721.86


CAS No. 537049-40-4
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID