Tubacin

PubMed: 26783207      Nat Commun

BT474 cells were daily treated with Tubacin at the indicated concentration for 2 days, and harvested for western blot analysis.

PubMed: 26643866      Exp Biol Med (Maywood)

Tubacin leads to an increase in HDAC6 foci in PC3 cells. PC3 cells were treated with control (DMSO) or tubacin for 24 h prior to processing for HDAC6 (red) and p62 (green) visualization by confocal microscopy. HDAC6 formed cytoplasmic foci in response to tubacin. HDAC6 foci strongly colocalized with p62 foci. Scale bar is 10 µm.

PubMed: 25311840      Protein Cell

Immunofluorescence images of HeLa cells treated with DMSO or tubacin and stained with the anti-MYH9 antibody, phalloidin, and DAPI. 

PubMed: 25031336      J Virol

(A to F) Effect of tubacin treatment on the intracellular and cell surface distribution of HA. A549 cells were infected with PR8 at an MOI of 1.0 and either mock treated (−Tubacin) or treated with 10 μM tubacin (+Tubacin). After 24 h, cells were fixed, permeabilized, and stained with mouse anti-acetylated α-tubulin followed by Alexa 594-conjugated rabbit anti-mouse IgG antibody. Cells were then stained with goat anti-HA followed by Alexa 488-conjugated rabbit anti-goat IgG antibody (left and middle columns). Cells shown in the right column were stained without permeabilization. (G and H) Mock-infected cells were stained as described above. Finally, cells were stained with the DNA-binding dye Hoechst and analyzed by confocal microscopy. Scale bar, 10 μm. (I to K) Quantification of the cell-surface HA by flow cytometry. A549 cells were treated as follows: infected with PR8 at an MOI of 1.0 and mock treated or treated with 10 μM tubacin (Tub)

PubMed: 29845122      Open Med (Wars)

Human glioma cell lines LN229 and U251 were plated in 96-well plates (5×103/well) and cultured at 37°C for 24 hours. Then cells were treated with or without various concentrations of tubacin for 8 hours. Cell growth was monitored by crystal violet staining.