Tubacin

Catalog No.S2239

Tubacin Chemical Structure

Molecular Weight(MW): 721.86

Tubacin is a highly potent and selective, reversible, cell-permeable HDAC6 inhibitor with an IC50 of 4 nM in a cell-free assay, approximately 350-fold selectivity over HDAC1.

Size Price Stock Quantity  
USD 370 In stock
USD 670 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Cited by 11 Publications

3 Customer Reviews

  • Verification of Hdac6 deletion in knockout MEFs. Expression of HDAC6 and acetylation of tubulin were analyzed by immunoblotting.

    Nat Biotechnol, 2015, 33(4): 415-23 . Tubacin purchased from Selleck.

    Inhibition of HDAC6 by tubacin induced the acetylation and membrane translocation of wild-type PTEN, but not K163R mutant. U-87 MG or NCl-H1650 cells were transfected with EGFP-PTEN or EGFP-PTEN-K163R for 24 h and then treated with tubacin for 24 h. *P<0.01.

    Oncogene, 2016, 35(18):2333-44. Tubacin purchased from Selleck.

  • A, immunoblotting analysis of E-cadherin, ZO-1, Vimentin, N-cadherin, acetyl-α-tubulin, and α-tubulin in MCF-10A cells. Cells were treated with SB431542 (5 μM), Tubacin (5 μM), Paclitaxol (PTX) (5 nM), Nocodazol (NDL) (5 nM), TSA (50 ng/liter), or Nicotiamide (Nico) (10 mM), followed by co-incubation with TGF-β (2 ng/ml).

    J Biol Chem, 2016, 291(10):5396-405. Tubacin purchased from Selleck.

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Tubacin is a highly potent and selective, reversible, cell-permeable HDAC6 inhibitor with an IC50 of 4 nM in a cell-free assay, approximately 350-fold selectivity over HDAC1.
Features The first known selective inhibitor of α-tubulin deacetylation.
Targets
HDAC6 [1]
(Cell-free assay)
4 nM
In vitro

Tubacin, without directly stabilizing microtubules, induces an increase in α-tubulin acetylation with EC50 of 2.5 μM in A549 cells. Tubacin inhibits HDAC6-mediated α-tubulin deacetylation, and inhibits the migration of both wild-type and HDAC6-overexpressing cells. [2] Tubacin, in combination with paclitaxel, synergistically enhances tubulin acetylation. [3] Tubacin significantly inhibits both drug-sensitive and drug–resistant MM cell growth with IC50 of 5–20 μM, and induces cell apoptosis by activation of caspases. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human HeLa cells M4\hW2Z2dmO2aX;uJIF{e2G7 MV:2JIg> NUnTV3lsUW6qaXLpeIlwdiCxZjDISGFEPiCrbjDoeY1idiCKZVzhJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBqdiCNNECgbJlx\XKjY3X0fYxifGmxbjDv[kBidHCqYT30eYJ2dGmwIHnuZ5Vj[XSnZDDmc5IhPiCqcoOgZpkhcW2vdX7v[ox2d3Knc3PlcoNmKGG|c3H5MEBKSzVyPUKuPUDPxE1? MXuyOVQ2PDJ5MB?=
human A549 cells M{fuNGZ2dmO2aX;uJIF{e2G7 MVnJcohq[mm2aX;uJI9nKEiGQVO2JIlvKGi3bXHuJGE2PDliY3XscJMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFteGijLYT1ZpVtcW5iYXPleJlt[XSrb36gZpkh\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcImsJGVEPTB;Mj65JO69VQ>? MXWxOlQxQDByMx?=
human T24 cells NFXyXHpHfW6ldHnvckBie3OjeR?= NWn0WYZXUW6mdXP0bY9vKG:oIHjpd5RwdmViSEOgZYNmfHmuYYTpc44hcW5iaIXtZY4hXDJ2IHPlcIx{NCCHQ{WwQVIvQSEQvF2= MlP6NVkyOTF2Nk[=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Acetyl-α-tubulin / α-tubulin / pERK / ERK / p-MLC / MLC; 

PubMed: 26783207     


BT474 cells were daily treated with Tubacin at the indicated concentration for 2 days, and harvested for western blot analysis.

26783207
Immunofluorescence
p62 / HDAC6 ; 

PubMed: 26643866     


Tubacin leads to an increase in HDAC6 foci in PC3 cells. PC3 cells were treated with control (DMSO) or tubacin for 24 h prior to processing for HDAC6 (red) and p62 (green) visualization by confocal microscopy. HDAC6 formed cytoplasmic foci in response to tubacin. HDAC6 foci strongly colocalized with p62 foci. Scale bar is 10 µm.

Phalloidin / MYH9 ; 

PubMed: 25311840     


Immunofluorescence images of HeLa cells treated with DMSO or tubacin and stained with the anti-MYH9 antibody, phalloidin, and DAPI. 

Acetylated Microtubules / Viral HA ; 

PubMed: 25031336     


(A to F) Effect of tubacin treatment on the intracellular and cell surface distribution of HA. A549 cells were infected with PR8 at an MOI of 1.0 and either mock treated (−Tubacin) or treated with 10 μM tubacin (+Tubacin). After 24 h, cells were fixed, permeabilized, and stained with mouse anti-acetylated α-tubulin followed by Alexa 594-conjugated rabbit anti-mouse IgG antibody. Cells were then stained with goat anti-HA followed by Alexa 488-conjugated rabbit anti-goat IgG antibody (left and middle columns). Cells shown in the right column were stained without permeabilization. (G and H) Mock-infected cells were stained as described above. Finally, cells were stained with the DNA-binding dye Hoechst and analyzed by confocal microscopy. Scale bar, 10 μm. (I to K) Quantification of the cell-surface HA by flow cytometry. A549 cells were treated as follows: infected with PR8 at an MOI of 1.0 and mock treated or treated with 10 μM tubacin (Tub)

26643866 25311840 25031336
Growth inhibition assay
Cell viability; 

PubMed: 29845122     


Human glioma cell lines LN229 and U251 were plated in 96-well plates (5×103/well) and cultured at 37°C for 24 hours. Then cells were treated with or without various concentrations of tubacin for 8 hours. Cell growth was monitored by crystal violet staining. 

29845122
In vivo In chick embryos, inhibition of HDAC6 activity by Tubacin reduces the formation of new blood vessels in matrigel/nylon mesh. In angioreactors implanted in mice, Tubacin also impairs the formation of new blood vessels. [5]

Protocol

Kinase Assay:

[1]

+ Expand

Enzyme Inhibition Assay:

Enzyme inhibition assays are performed using the Reaction Biology HDAC Spectrum platform. The HDAC1, 2, 4, 5, 6, 7, 8, 9, 10, and 11 assays used isolated recombinant human protein; HDAC3/NcoR2 complex is used for the HDAC3 assay. Substrate for HDAC1, 2, 3, 6, 10, and 11 assays is a fluorogenic peptide from p53 residues 379-382 (RHKKAc); substrate for HDAC8 is fluorogenic diacyl peptide based on residues 379-382 of p53 (RHKAcKAc). Acetyl-Lys(trifluoroacetyl)-AMC substrate is used for HDAC4, 5, 7, and 9 assays. Compounds are dissolved in DMSO and tested in 10-dose IC50 mode with 3-fold serial dilution starting at 30 μM. Control Compound Trichostatin A (TSA) is tested in a 10-dose IC50 with 3-fold serial dilution starting at 5 μM. IC50 values are extracted by curve-fitting the dose/response slopes.
Cell Research:

[4]

+ Expand
  • Cell lines: Drug-sensitive (MM.1S, U266, INA-6, and RPMI8226) and drug-resistant (RPMI-LR5 and RPMI-Dox40) MM cell lines
  • Concentrations: ~20 μM
  • Incubation Time: 72 hours
  • Method:

    The inhibitory effect of bortezomib and/or tubacin on MM cell growth is assessed by measuring 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye absorbance. All experiments are performed in quadruplicate.


    (Only for Reference)
Animal Research:

[5]

+ Expand
  • Animal Models: Athymic nude mice implanted with angioreactors
  • Formulation: DMSO
  • Dosages: --
  • Administration: Tubacin is filled in semiclosed angioreactors, and then implanted into the mice.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (138.53 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
10mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 721.86
Formula

C41H43N3O7S

CAS No. 537049-40-4
Storage powder
in solvent
Synonyms N/A

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

HDAC Signaling Pathway Map

Related HDAC Products

Tags: buy Tubacin | Tubacin supplier | purchase Tubacin | Tubacin cost | Tubacin manufacturer | order Tubacin | Tubacin distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID