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Liproxstatin-1 Ferroptosis inhibitor

Name: Liproxstatin-1
SKU: S7699
Availability: InStock
Rating: 5 (275 reviews)

Cat.No.S7699

Liproxstatin-1 is a potent ferroptosis inhibitor with an IC50 of 22 nM.

Chemical Structure

Molecular Weight: 340.85

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.88%

99.88

Products Often Used Together with Liproxstatin-1

RSL3

It and RSL3, in combination with iFSP1, induce cell death in human esophageal squamous cell carcinoma (ESCC) cell lines KYSE30/KYSE510/KYSE520.

DHA (Dihydroartemisinin)

It and Dihydroartemisinin (DHA) have an antagonistic effect on the growth of blood-stage parasites of malaria.

iFSP1

It and RSL3 combination induce cell death in human esophageal squamous cell carcinoma (ESCC) cell lines KYSE30/KYSE510/KYSE520.

S63845

This compound completely protects BAX/BAK double knock-out (DKO) U251 cells from cell death induced by the combination of S63845 and A1331852.

A-1331852

It effectively reduces cell death of U251 cells when treated with A1331852 plus S63845.

Related Targets	P450 (e.g. CYP17) Dehydrogenase PDE phosphatase PPAR HSP Vitamin Transferase Carbohydrate Metabolism Mitochondrial Metabolism Casein Kinase Serine/threonin kinase Lipoxygenase Phospholipase (e.g. PLA) Retinoid Receptor DPP ACE Peroxidases Amino Acids and Derivatives Fatty Acid Synthase FXR HMG-CoA Reductase Carbonic Anhydrase Lipase Decarboxylase DHFR IDO/TDO Hydroxylase PCSK9 OXPHOS
Related Products	Ferrostatin-1 (Fer-1) Erastin RSL3 FIN56 IKE (Imidazole Ketone Erastin) UAMC-3203 iFSP1 SRS16-86 SRS11-92 Erastin2 NPD4928 icFSP1 Ferric citrate Butylhydroxyanisole GPX4-IN-5 FSEN1 N6F11

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
MRC-5 lung fibroblast cells					DNDI: Cytotoxicity against human MRC-5 lung fibroblast cells, IC50 = 9.14 μM	ChEMBL
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	340.85	Formula	C₁₉H₂₁ClN₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	950455-15-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	N/A			Smiles	C1CNCCC12C(=NCC3=CC(=CC=C3)Cl)NC4=CC=CC=C4N2

Solubility

In vitro
Batch:

DMSO : 68 mg/mL (199.5 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 18 mg/mL

Water : Insoluble

DMSO : 625 mg/mL (1833.65 mM) Warmed with 50°C water bath; Ultrasonicated;
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 34 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	2%DMSO 1 40%PEG300 2 2%Tween80 3 56%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.000mg/ml (8.80mM)	Taking the 1 mL working solution as an example, add 20 μL of 150 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 20 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 560 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.000mg/ml (2.93mM)	Taking the 1 mL working solution as an example, add 50 μL of 20 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	ferroptosis ^[1] (Cell-free assay) 22 nM
In vitro	Liproxstatin-1 is able to inhibit ferroptosis in the low nanomolar range and inhibit the growth of Gpx4^−/−cells with IC50 of 22 nM. This compound (50 nM) completely prevents lipid peroxidation in Gpx4^−/−cells. It (200 nM) protects against FINs, such as BSO (10 µM), erastin (1 µM) and RSL3 (0.5 µM), in a dose dependent manner, whereas it fails to rescue cell death induced by staurosporine (0.2 µM) and H₂O₂ (200 µM).^[1]
In vivo	Liproxstatin-1 remarkably extends survival compared with the vehicle-treated group, delays ferroptosis in tubular cells, and mitigates tissue injury in ischaemia/reperfusion-induced liver injury. ^[1]
References	[1] https://pubmed.ncbi.nlm.nih.gov/25402683/

Applications

Methods	Biomarkers	PMID
Western blot	COX2 / GPx4 / GAPDH β-actin / COX2 / GPx4 β-actin / COX2 / GPx4 NeuN / Syn / SNAP / GFAP / Actin / Tubulin GRP78 / CHOP / Actin GRP78 / CHOP / Actin GPX4 / β-actin	33889074
Growth inhibition assay	Cell viability	31641008
Immunofluorescence	β-actin / COX2 / GPx4 NeuN / ACSL4 mitochondrial Fe2+	30737476

Tech Support

Handling Instructions

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