Pracinostat (SB939)

Catalog No.S1515

Pracinostat (SB939) Chemical Structure

Molecular Weight(MW): 358.48

Pracinostat (SB939) is a potent pan-HDAC inhibitor with IC50 of 40-140 nM with exception for HDAC6. It has no activity against the class III isoenzyme SIRT I. Phase 2.

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In DMSO USD 300 In stock
USD 210 In stock
USD 370 In stock
USD 870 In stock
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Cited by 8 Publications

5 Customer Reviews

  • Hyperacetylation of P. falciparum proteins by SB939. Synchronous 3D7 trophozoite-stage P. falciparum parasites were treated with 50 or 500 nM chloroquine (CQ), SAHA, or SB939 or with vehicle only (control; 0.05% DMSO) for 3 h. Following saponin lysis, parasite protein lysates were prepared and SDS-PAGE and Western blotting carried out using anti-acetyl H4 or anti-pan-acetyl lysine (K103) antibodies. Coomassie blue staining was carried out as a loading control.

    Antimicrob Agents Chemother 2012 56(7), 3849-56. Pracinostat (SB939) purchased from Selleck.

    Breast cancer cells were treated with the indicated concentrations of SB939.



    Dr. Zhang of Tianjin Medical University. Pracinostat (SB939) purchased from Selleck.

  • A significant decrease in detectable PLA signal following HDAC inhibition in SYO-1 cells A, B. is also confirmed by immunoprecipitation C. The decrease in PLA co-localization signal correlates with apoptosis induction by HDAC inhibitor FK228 in SYO-1 cells.

    Oncotarget, 2016, 7(23):34384-94. Pracinostat (SB939) purchased from Selleck.

    (B) Immunoblots showing induction of apoptosis in K562 BIMi2+/+, BIMi2+/- and BIMi2-/-. The data shown are representative of 3 experiments with similar results. Cells were treated for 48hours with SB939 at the indicated concentrations. p-BCR-ABL, phosphorylated BCR-ABL; STAT5, signal transducer and activator of transcription 5; p-STAT5, phosphorylated STAT5; BIMEL, BIM extra long isoform; BIML, BIM long isoform; Cl. CASP3, cleaved CASPASE3; Cl. PARP, cleaved PARP. 48-hour treatment as in A.

    PLoS One, 2017, 12(3):e0174107. Pracinostat (SB939) purchased from Selleck.

  • A: Mean fold change in γ-globin mRNA in erythroid progenitor cells treated with the designated therapeutic candidates (MS-275, SDMB, SB939, or Benserazide), compared to vehicle-treated cells from the same subject. All changes are significant, p <0.001. Error bars indicate SD. B. Mean change in proportions of cells expressing HbF protein (F-reticulocytes) in erythroid progenitor cells treated with therapeutic candidates (SDMB, Benserazide, MS275, SB939), compared to control cells from the same subject. Error bars indicate SD. * Asterisks indicate statistically significant differences (* p<0.05, ** p<0.01. *** p<0.001)

    Blood Cells Mol Dis, 2016, 56(1):62-9. Pracinostat (SB939) purchased from Selleck.

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Pracinostat (SB939) is a potent pan-HDAC inhibitor with IC50 of 40-140 nM with exception for HDAC6. It has no activity against the class III isoenzyme SIRT I. Phase 2.
Features A new histone deacetylase inhibitor based on hydroxamic acid, with improved physicochemical, pharmaceutical, and pharmacokinetic properties.
HDAC10 [1]
(Cell-free assay)
HDAC3 [1]
(Cell-free assay)
HDAC5 [1]
(Cell-free assay)
HDAC1 [1]
(Cell-free assay)
HDAC4 [1]
(Cell-free assay)
40 nM 43 nM 47 nM 49 nM 56 nM
In vitro

SB939 has a 100-fold greater selectivity for HDACs than for Zn-binding non-HDAC enzymes, receptors, and ion channels. SB939 is a potent inhibitor of HDAC class I isoenzymes, HDAC1, HDAC2, HDAC3 and HDAC8 with the IC50 values ranging from 43 nM to 140 nM. SB939 inhibits HDAC class II isoenzymes , HDAC4, HDAC5, HDAC7, HDAC9 and HDAC10 significantly with the IC50 values ranging from 40 nM to 137 nM, with the exception of HDAC6 which shows IC50 of 1008 nM. It markedly inhibits HDAC11 of the HDAC class IV enzymes with IC50 of 93 nM, but shows no inhibitory activity against SIRT 1 of the class III HDACs. SB939 shows significant antiproliferative activity against a wide variety of tumor cell lines, especially Leukemia cells and cutaneous T-cell Lymphoma cells with IC50 values ranging from 50 nM (H9 cells) to 170 nM (HEL92.1.7 cells). [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 cells Ml3FVJJwdGmoZYLheIlwdiCjc4PhfS=> NGC3VII1QCCq MmXjRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBIUTVyPUCuNVch|ryP M1u2U|I1OTF7NUW1
NCI-H460 cells MX3Qdo9tcW[ncnH0bY9vKGG|c3H5 NVr5dog4PDhiaB?= NV\KXI5WSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDOR2kuUDR4MDDj[YxteyCjZoTldkA1QCCqcoOgZpkhe3WuZn;ybI9l[W2rbnWgRkBie3OjeTygS2k2OD1yLkKyJO69VQ>? M3TycVI1OTF7NUW1
HCT116 cells MULQdo9tcW[ncnH0bY9vKGG|c3H5 MlTaOFghcA>? NV:xOod5SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIR3QyOTZiY3XscJMh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFfJOVA:OC5{NDFOwG0> NFTPPJQzPDFzOUW1OS=>
MDA-MB-435 cells MmjEVJJwdGmoZYLheIlwdiCjc4PhfS=> M1jLWVQ5KGh? NH[5PY5CdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIh2dWGwIF3ERU1OSi12M{WgZ4VtdHNiYX\0[ZIhPDhiaILzJIJ6KHO3bH\vdohw\GGvaX7lJGIh[XO|YYmsJGdKPTB;MD60PEDPxE1? M1rxclI1OTF7NUW1
OVCAR5 cells MXfQdo9tcW[ncnH0bY9vKGG|c3H5 MXe0PEBp M1vUVGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4SgbJVu[W5iT2\DRXI2KGOnbHzzJIFnfGW{IES4JIhzeyCkeTDzeYxnd3Kqb3ThcYlv\SCEIHHzd4F6NCCJSUWwQVAvPjFizszN M1PzWFI1OTF7NUW1

... Click to View More Cell Line Experimental Data

In vivo Administration of SB939 (25 mg/kg to 100 mg/kg) displays a dose-dependent antitumor efficacy in a xenograft mice model of human colorectal cancer (HCT-116). This is approximately twice as efficacious as SAHA: SB939 causing a tumor growth inhibition of 94% versus 48% by SAHA with both at the maximum tolerated dose. Oral administration of SB939 at a dose of 50 mg/kg or 75 mg/kg in the APCmin genetic mice model of early-stage colon cancer markedly reduces the number of tumors , decreases cumulative hemocult scores and increases hematocrit values more effectively than 5-fluorouracil. [1]


Kinase Assay:[1]
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HDAC enzyme assay:

All recombinant HDAC enzymes, with the exception of SIRT1, are cloned and expressed in S*BIO. The reaction mix containing 2.5 or 5 μL of the HDAC isoenzyme, assay buffer (25 mM Tris-HCl, pH 7.5; 137 mM NaCl; 2.7 mM KCl, 1 mM MgCl2 and 1 mg/mL BSA), different concentrations of SB939, and the fluorogenic deacetylase substrate Flour de LysTM in a total reaction volume of 33 μL is incubated at room temperature for 2 hours. 16 μL of Flour de LysTM developer is added and incubated for an additional 10 minutes. The emitted light is measured at 460 nm in a microplate reader. IC50 values are generated using the XLfit software.
Cell Research:[1]
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  • Cell lines: HCT116, A2780, ACHN, MCF7, HL-60, et al.
  • Concentrations: Dissolved in DMSO (stock concentration, 10 mM), final concentrations 1.5 nM to 100 μM
  • Incubation Time: 96 hours
  • Method: Cells are seeded in 96-well plates in the log growth phase at a predetermined optimal density, and rested for 24 hours (adherent cells) or 2 hours (suspension cells), respectively. They are exposed to different concentrations of SB939 for 96 hours. Cell proliferation assays are done using either the CyQUANT cell proliferation assay kit for adherent cells or the CellTiter96 Aqueous One solution cell proliferation kit for suspension cells.
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: BALB/c nude mice bearing HCT-116 human colon cancer xenografts, Male and APCmin mice
  • Formulation: Dissolved in DMSO and prepared in 0.5% methylcellulose (w/v) and 0.1% Tween-80 in water
  • Dosages: 25, 50, 75, or 100 mg/kg
  • Administration: Oral gavage once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 72 mg/mL (200.84 mM)
Ethanol 27 mg/mL (75.31 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% DMSO+30% polyethylene glycol+1% Tween 80
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 358.48


CAS No. 929016-96-6
Storage powder
in solvent
Synonyms N/A

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03848754 Not yet recruiting Relapsed Adult AML Medical College of Wisconsin March 2019 Phase 1
NCT03848754 Not yet recruiting Relapsed Adult AML Medical College of Wisconsin March 2019 Phase 1
NCT03495934 Enrolling by invitation Healthy Subjects Helsinn Healthcare SA|Quotient Sciences February 8 2018 Phase 1
NCT03495934 Enrolling by invitation Healthy Subjects Helsinn Healthcare SA|Quotient Sciences February 8 2018 Phase 1
NCT03151304 Active not recruiting Myelodysplastic Syndromes Helsinn Healthcare SA June 1 2017 Phase 2
NCT03151408 Recruiting Acute Myeloid Leukemia Helsinn Healthcare SA|Clinipace Worldwide June 23 2017 Phase 3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID