Pracinostat (SB939)

For research use only.

Catalog No.S1515

17 publications

Pracinostat (SB939) Chemical Structure

CAS No. 929016-96-6

Pracinostat (SB939) is a potent pan-HDAC inhibitor with IC50 of 40-140 nM with exception for HDAC6. It has no activity against the class III isoenzyme SIRT I. Pracinostat (SB939) induces apoptosis in tumor cells. Phase 2.

Size Price Stock Quantity  
10mM (1mL in DMSO) USD 300 In stock
USD 117 In stock
USD 210 In stock
USD 370 In stock
USD 870 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's Pracinostat (SB939) has been cited by 17 publications

5 Customer Reviews

  • Hyperacetylation of P. falciparum proteins by SB939. Synchronous 3D7 trophozoite-stage P. falciparum parasites were treated with 50 or 500 nM chloroquine (CQ), SAHA, or SB939 or with vehicle only (control; 0.05% DMSO) for 3 h. Following saponin lysis, parasite protein lysates were prepared and SDS-PAGE and Western blotting carried out using anti-acetyl H4 or anti-pan-acetyl lysine (K103) antibodies. Coomassie blue staining was carried out as a loading control.

    Antimicrob Agents Chemother 2012 56(7), 3849-56. Pracinostat (SB939) purchased from Selleck.

  • Breast cancer cells were treated with the indicated concentrations of SB939.



    Dr. Zhang of Tianjin Medical University. Pracinostat (SB939) purchased from Selleck.

  • A significant decrease in detectable PLA signal following HDAC inhibition in SYO-1 cells A, B. is also confirmed by immunoprecipitation C. The decrease in PLA co-localization signal correlates with apoptosis induction by HDAC inhibitor FK228 in SYO-1 cells.

    Oncotarget, 2016, 7(23):34384-94. Pracinostat (SB939) purchased from Selleck.

  • (B) Immunoblots showing induction of apoptosis in K562 BIMi2+/+, BIMi2+/- and BIMi2-/-. The data shown are representative of 3 experiments with similar results. Cells were treated for 48hours with SB939 at the indicated concentrations. p-BCR-ABL, phosphorylated BCR-ABL; STAT5, signal transducer and activator of transcription 5; p-STAT5, phosphorylated STAT5; BIMEL, BIM extra long isoform; BIML, BIM long isoform; Cl. CASP3, cleaved CASPASE3; Cl. PARP, cleaved PARP. 48-hour treatment as in A.

    PLoS One, 2017, 12(3):e0174107. Pracinostat (SB939) purchased from Selleck.

  • A: Mean fold change in γ-globin mRNA in erythroid progenitor cells treated with the designated therapeutic candidates (MS-275, SDMB, SB939, or Benserazide), compared to vehicle-treated cells from the same subject. All changes are significant, p <0.001. Error bars indicate SD. B. Mean change in proportions of cells expressing HbF protein (F-reticulocytes) in erythroid progenitor cells treated with therapeutic candidates (SDMB, Benserazide, MS275, SB939), compared to control cells from the same subject. Error bars indicate SD. * Asterisks indicate statistically significant differences (* p<0.05, ** p<0.01. *** p<0.001)

    Blood Cells Mol Dis, 2016, 56(1):62-9. Pracinostat (SB939) purchased from Selleck.

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Pracinostat (SB939) is a potent pan-HDAC inhibitor with IC50 of 40-140 nM with exception for HDAC6. It has no activity against the class III isoenzyme SIRT I. Pracinostat (SB939) induces apoptosis in tumor cells. Phase 2.
Features A new histone deacetylase inhibitor based on hydroxamic acid, with improved physicochemical, pharmaceutical, and pharmacokinetic properties.
HDAC10 [1]
(Cell-free assay)
HDAC3 [1]
(Cell-free assay)
HDAC5 [1]
(Cell-free assay)
HDAC1 [1]
(Cell-free assay)
HDAC4 [1]
(Cell-free assay)
40 nM 43 nM 47 nM 49 nM 56 nM
In vitro

SB939 has a 100-fold greater selectivity for HDACs than for Zn-binding non-HDAC enzymes, receptors, and ion channels. SB939 is a potent inhibitor of HDAC class I isoenzymes, HDAC1, HDAC2, HDAC3 and HDAC8 with the IC50 values ranging from 43 nM to 140 nM. SB939 inhibits HDAC class II isoenzymes , HDAC4, HDAC5, HDAC7, HDAC9 and HDAC10 significantly with the IC50 values ranging from 40 nM to 137 nM, with the exception of HDAC6 which shows IC50 of 1008 nM. It markedly inhibits HDAC11 of the HDAC class IV enzymes with IC50 of 93 nM, but shows no inhibitory activity against SIRT 1 of the class III HDACs. SB939 shows significant antiproliferative activity against a wide variety of tumor cell lines, especially Leukemia cells and cutaneous T-cell Lymphoma cells with IC50 values ranging from 50 nM (H9 cells) to 170 nM (HEL92.1.7 cells). [1]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
MCF7 cells MVTQdo9tcW[ncnH0bY9vKGG|c3H5 NGPvOZU1QCCq Mm\SRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCPQ1[3JINmdGy|IHHmeIVzKDR6IHjyd{BjgSC|dXzmc5Jpd2SjbXnu[UBDKGG|c3H5MEBIUTVyPUCuNVch|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFzOUW1OUc,OjRzMUm1OVU9N2F-
NCI-H460 cells MlTuVJJwdGmoZYLheIlwdiCjc4PhfS=> M1f1elQ5KGh? MkK0RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCQQ1mtTFQ3OCClZXzsd{Bi\nSncjC0PEBpenNiYomgd5Vt\m:{aH;kZY1qdmViQjDhd5NigSxiR1m1NF0xNjJ{IN88US=> NWTX[5VNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
HCT116 cells MnuwVJJwdGmoZYLheIlwdiCjc4PhfS=> MoPOOFghcA>? MXHBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEiFVEGxOkBk\WyuczDh[pRmeiB2ODDodpMh[nlic4Xs[o9zcG:mYX3pcoUhSiCjc4PhfUwhT0l3ME2wMlI1KM7:TR?= NF3idFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGxPVU2PSd-MkSxNVk2PTV:L3G+
MDA-MB-435 cells NH;oUm5Rem:uaX\ldoF1cW:wIHHzd4F6 NYT5cWhYPDhiaB?= MULBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKE2GQT3NRk01OzViY3XscJMh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFfJOVA:OC52ODFOwG0> NF;rS2c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGxPVU2PSd-MkSxNVk2PTV:L3G+
OVCAR5 cells MV7Qdo9tcW[ncnH0bY9vKGG|c3H5 MWe0PEBp NUWxNIFZSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDPWmNCWjViY3XscJMh[W[2ZYKgOFghcHK|IHL5JJN2dG[xcnjv[IFucW6nIFKgZZN{[XluIFfJOVA:OC54MTFOwG0> NWTT[3VoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
HepG2 M4nsXWFvfGmvYXzhdolidCCjc4PhfS=> NGLIRZlCdnSrbXHsZZJq[WxiYXP0bZZqfHliYXfhbY5{fCCneH:t[ZJ6fGi{b3P5eIlkKG[xcn2gc4YhWGyjc33v[Il2dSCkZYLnbIVqKGmwZnXjeIVlKGmwIHj1cYFvKEincFeyJINmdGy|LDDJR|UxKD1iMD6xOUDPxE1w MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyNEm2O{c,OjR7MES5Olc9N2F-
HepG2 NUDsZVR3SW62aYDsZZNud2SrYXygZZN{[Xl? MUK1N{BpenN? M4O1eWFvfGmybHHzcY9lcWGuIHHjeIl3cXS7IHHnZYlve3RiZYjv[ZJ6fGi{b3P5eIlkNXO2YXflJI9nKFCuYYPtc4RqfW1iYnXy[4hmcSCDTlvBJIlv\mWldHXkJIlvKGi3bXHuJGhmeEd{IHPlcIx{KGGodHXyJFU{KGi{czDifUBFSVCLIIP0ZYlvcW6pLXLhd4VlKG2ndHjv[EwhUUN3MDC9JFAvOTVizszNMi=> M2XN[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6MkSxNVEzLz5{OEK0NVEyOjxxYU6=
Sf9 MWLGeY5kfGmxbjDhd5NigQ>? NUnQSndyOiCqcoO= MWLJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEiGQVOtNkBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgbY5n\WO2ZXSgbY5{\WO2IGPmPUBk\WyuczD1d4lv\yCIbIXvdkBl\SCOeYOgZZMhe3Wkc4TyZZRmKHC{ZXnuZ5Vj[XSnZDDmc5IhOiCqcoOg[o9tdG:5ZXSgZpkhe3Wkc4TyZZRmKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFExKG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCNaTC9JFAvODF4IN88UU4> M4nKelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFE5PTF7NEOvK|5EcEWPQly8M4E,
HEK293-F MlTMSpVv[3Srb36gZZN{[Xl? Mk[xNkBpenN? MVnJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEiGQVOtPEBmgHC{ZYPz[YQhcW5iSFXLNlk{NUZiY3XscJMhfXOrbnegSox2d3JiZHWgUJl{KGG|IIP1ZpN1emG2ZTDwdoVqdmO3YnH0[YQh\m:{IEKgbJJ{KG[xbHzve4VlKGK7IIP1ZpN1emG2ZTDh[IRqfGmxbjDt[YF{fXKnZDDh[pRmeiBzMDDtbY5{KGK7IH\seY9z\XOlZX7j[UBie3OjeTygT4khRSByLkCxOkDPxE1w NUPrOGttRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOTh3MUm0N{8oRkOqRV3CUFww[T5?
Sf9 M{HjZ2Z2dmO2aX;uJIF{e2G7 MXeyJIhzew>? MUDJcohq[mm2aX;uJI9nKHKnY3;tZolv[W62IHj1cYFvKEiGQVOtNUBmgHC{ZYPz[YQhcW5iYnHjeYxwfmm{dYOgbY5n\WO2ZXSgbY5{\WO2IGPmPUBk\WyuczD1d4lv\yCIbIXvdkBl\SCOeYOgZZMhe3Wkc4TyZZRmKHC{ZXnuZ5Vj[XSnZDDmc5IhOiCqcoOg[o9tdG:5ZXSgZpkhe3Wkc4TyZZRmKGGmZHn0bY9vKG2nYYP1doVlKGGodHXyJFExKG2rboOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCNaTC9JFAvODF4IN88UU4> NEjWOWE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwyQDVzOUSzM{c,S2iHTVLMQE9iRg>?

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot
p-JAK2 / JAK2 / p-STAT5 / STAT5 / p-FLT3 / FLT3 ; 

PubMed: 22829971     

Western blot analyses from 25 or 50 μg (pFLT3 and FLT3 blots only) of total cell lysate of the indicated cell lines with JAK2V617F, JAK2, or FLT3-ITD or FLT3 wt are shown. (a, b) Cells were treated with the indicated concentrations of pracinostat (SB939) for 24 h.

In vivo Administration of SB939 (25 mg/kg to 100 mg/kg) displays a dose-dependent antitumor efficacy in a xenograft mice model of human colorectal cancer (HCT-116). This is approximately twice as efficacious as SAHA: SB939 causing a tumor growth inhibition of 94% versus 48% by SAHA with both at the maximum tolerated dose. Oral administration of SB939 at a dose of 50 mg/kg or 75 mg/kg in the APCmin genetic mice model of early-stage colon cancer markedly reduces the number of tumors , decreases cumulative hemocult scores and increases hematocrit values more effectively than 5-fluorouracil. [1]


Kinase Assay:[1]
- Collapse

HDAC enzyme assay:

All recombinant HDAC enzymes, with the exception of SIRT1, are cloned and expressed in S*BIO. The reaction mix containing 2.5 or 5 μL of the HDAC isoenzyme, assay buffer (25 mM Tris-HCl, pH 7.5; 137 mM NaCl; 2.7 mM KCl, 1 mM MgCl2 and 1 mg/mL BSA), different concentrations of SB939, and the fluorogenic deacetylase substrate Flour de LysTM in a total reaction volume of 33 μL is incubated at room temperature for 2 hours. 16 μL of Flour de LysTM developer is added and incubated for an additional 10 minutes. The emitted light is measured at 460 nm in a microplate reader. IC50 values are generated using the XLfit software.
Cell Research:[1]
- Collapse
  • Cell lines: HCT116, A2780, ACHN, MCF7, HL-60, et al.
  • Concentrations: Dissolved in DMSO (stock concentration, 10 mM), final concentrations 1.5 nM to 100 μM
  • Incubation Time: 96 hours
  • Method: Cells are seeded in 96-well plates in the log growth phase at a predetermined optimal density, and rested for 24 hours (adherent cells) or 2 hours (suspension cells), respectively. They are exposed to different concentrations of SB939 for 96 hours. Cell proliferation assays are done using either the CyQUANT cell proliferation assay kit for adherent cells or the CellTiter96 Aqueous One solution cell proliferation kit for suspension cells.
    (Only for Reference)
Animal Research:[1]
- Collapse
  • Animal Models: BALB/c nude mice bearing HCT-116 human colon cancer xenografts, Male and APCmin mice
  • Dosages: 25, 50, 75, or 100 mg/kg
  • Administration: Oral gavage once daily
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 72 mg/mL (200.84 mM)
Ethanol 27 mg/mL (75.31 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
1% DMSO+30% polyethylene glycol+1% Tween 80
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 358.48


CAS No. 929016-96-6
Storage powder
in solvent
Synonyms N/A

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02118909 Completed Drug: Pracinostat|Drug: Itraconazole|Drug: Ciprofloxacin Healthy Volunteers|Non-smokers Helsinn Healthcare SA May 2014 Phase 1
NCT02058784 Completed Drug: pracinostat Healthy Volunteers|Moderate to Heavy Smokers|Non-smokers Helsinn Healthcare SA|Celerion February 2014 Early Phase 1
NCT01112384 Completed Drug: SB939 Metastatic Sarcoma NCIC Clinical Trials Group|S*BIO|Canadian Cancer Trials Group March 18 2010 Phase 2
NCT00741234 Completed Drug: SB939|Drug: Azacitidine Solid Tumors|Hematologic Malignancies|Myelodysplastic Syndrome S*BIO April 2007 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

Related HDAC Products

Tags: buy Pracinostat (SB939) | Pracinostat (SB939) supplier | purchase Pracinostat (SB939) | Pracinostat (SB939) cost | Pracinostat (SB939) manufacturer | order Pracinostat (SB939) | Pracinostat (SB939) distributor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID