LMK-235

For research use only.

Catalog No.S7569

20 publications

LMK-235 Chemical Structure

CAS No. 1418033-25-6

LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.

Size Price Stock Quantity  
USD 137 In stock
USD 477 In stock
USD 957 In stock
Bulk Discount

Free Overnight Delivery on orders over $ 500
Next day delivery by 10:00 a.m. Order now.

Selleck's LMK-235 has been cited by 20 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.
Targets
HDAC5 [1]
(Cell-free assay)
HDAC4 [1]
(Cell-free assay)
4.2 nM 11.9 nM
In vitro

LMK-235 causes HDAC inhibition with IC50 of <1 μM in human cancer cell lines with different sensitivity towards cisplatin. In breast cancer cell line MDA-MB-231, tongue cancer cell line Cal27, and esophagus cell line Kyse510 cell line, LMK-235 displays a high cytotoxicity, and markedly enhances the cytotoxicity of cisplatin. [1] In addition, LMK-235 also shows nanomolar activity against multiple malaria parasite life cycle stages. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human A2780 cells MkH5R5l1d3SxeHnjbZR6KGG|c3H5 MkftO|IhcA>? NWW5cZZuS3m2b4TvfIlkcXS7IHHnZYlve3RiY3nzdIxifGmwIILld4l{fGGwdDDoeY1idiCDMke4NEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwM{Kg{txO MmrFNlMzPTJ4MEO=
human A2780 cells NHTOVmdEgXSxdH;4bYNqfHliYYPzZZk> MWe3NkBp MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuOFkh|ryP M2W1flI{OjV{NkCz
human A2780 cells MVvDfZRwfG:6aXPpeJkh[XO|YYm= MYmxPEBp NGm4NZFKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KG[udX;y[ZNk\W6lZTDhd5Nige,:jDDJR|UxRTBwNkWg{txO MVSyN|I2OjZyMx?=
human HepG2 cells NHr6RpNEgXSxdH;4bYNqfHliYYPzZZk> Mn[4OFghcA>? NYrqdphJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPDhiaILzMEBKSzZyPUGuNlYh|ryP NXXxUHNmOjR7MES5Olc>
human MDA-MB-231 cells NHLycZNEgXSxdH;4bYNqfHliYYPzZZk> MkO1O|IhcA>? MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBkcXOybHH0bY4he2Wwc3n0bZZmKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zLkO3JO69VQ>? M2LuRVI{OjV{NkCz
human KYSE-510 cells MV\DfZRwfG:6aXPpeJkh[XO|YYm= MWG3NkBp NW\HW2pQS3m2b4TvfIlkcXS7IHHnZYlve3RiY3nzdIxifGmwIILld4l{fGGwdDDoeY1idiCNWWPFMVUyOCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUKuOFgh|ryP NHjtZY0zOzJ3Mk[wNy=>

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:

[1]

- Collapse

HDAC IC50 Profiling:

The in vitro inhibitory activity of compounds against seven human HDAC isoforms (1, 2, 4 C2A, 5 C2A, 6, 8, and 11) are performed with a fluorescent based assay according to the company’s standard operating procedure. The IC50 values are determined using 10 different concentrations with 3-fold serial dilution starting at 10 μM. TSA and vorinostat are used as reference compounds.
Cell Research:

[1]

- Collapse
  • Cell lines: A2780, Cal27, Kyse510, and MDA-MB-231 cell lines
  • Concentrations: ~10 μM
  • Incubation Time: 72 hours
  • Method:

    The rate of cell survival under the action of test substances is evaluated by an improved MTT assay. The assay is based on the ability of viable cells to metabolize yellow MTT to violet formazan that can be detected spectrophotometrically. In brief, A2780, Cal27, Kyse510, and MDA-MB-231 cell lines are seeded at a density of 5000, 7000, 8000, and 10 000 cells/well in 96-well plates. After 24 h, cells are exposed to increased concentrations of the test compounds. Incubation is ended after 72 h, and cell survival is determined by addition of MTT solution (5 mg/mL in phosphate buffered saline). The formazan precipitate is dissolved in DMSO. Absorbance s measured at 544 and 690 nm in a FLUOstar microplate reader.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (197.04 mM)
Ethanol 58 mg/mL (197.04 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+30%PEG300+5%TW80+ddH2O
For best results, use promptly after mixing.
13mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 294.35
Formula

C15H22N2O4

CAS No. 1418033-25-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CC(=CC(=C1)C(=O)NOCCCCCC(=O)NO)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

HDAC Signaling Pathway Map

Related HDAC Products

Tags: buy LMK-235 | LMK-235 supplier | purchase LMK-235 | LMK-235 cost | LMK-235 manufacturer | order LMK-235 | LMK-235 distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID