LMK-235

For research use only.

Catalog No.S7569

27 publications

LMK-235 Chemical Structure

CAS No. 1418033-25-6

LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.

Selleck's LMK-235 has been cited by 27 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.
Targets
HDAC5 [1]
(Cell-free assay)
HDAC4 [1]
(Cell-free assay)
4.2 nM 11.9 nM
In vitro

LMK-235 causes HDAC inhibition with IC50 of <1 μM in human cancer cell lines with different sensitivity towards cisplatin. In breast cancer cell line MDA-MB-231, tongue cancer cell line Cal27, and esophagus cell line Kyse510 cell line, LMK-235 displays a high cytotoxicity, and markedly enhances the cytotoxicity of cisplatin. [1] In addition, LMK-235 also shows nanomolar activity against multiple malaria parasite life cycle stages. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780 NHXPeIREgXSxdH;4bYNqfHliYYPzZZk> M4PPZlczKGi{cx?= MkfrR5l1d3SxeHnjbZR6KGGpYXnud5Qh[2m|cHzheIlvKHKnc3nzeIFvfCCqdX3hckBCOjd6MDDj[YxteyCjZoTldkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVAvOzMQvF2= MnO0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
A2780 MWTGeY5kfGmxbjDhd5NigQ>? M33jOVE5KGi{cx?= NIfwU4dKdmirYnn0bY9vKG:oIFjERWMhcW5iY3nzdIxifGmwIILld4l{fGGwdDDoeY1idiCDMke4NEBk\WyuczDh[pRmeiBzODDodpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxRTBwM{NOwG0> NHrBT2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
DLD1 Ml\SSpVv[3Srb36gZZN{[Xl? MnHiNUB1dyBzMDD1US=> MnzqNlQhcHK| MWjJcohq[mm2aX;uJI9nKF[SU{O0JIlvKGi3bXHuJGRNTDFiY3XscJMh[XO|ZYPz[YQh[XNicILleoVvfGmxbjDv[kBPS0:DNDDwdo91\WmwIHTl[5Ji\GG2aX;uJIF1KDFidH:gNVAhfU1iaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NYDiO3V2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
DLD1 MkHZSpVv[3Srb36gZZN{[Xl? MoW1NUB1dyBzMDD1US=> NFvsfJYzPCCqcoO= M2j5bmlvcGmkaYTpc44hd2ZiVmDTN|QhcW5iaIXtZY4hTEyGMTDj[YxteyCjc4Pld5Nm\CCjczDwdoV3\W62aX;uJI9nKHB4MjDk[Ydz[WSjdHnvckBifCBzIITvJFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= NU\mR4lERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
DLD1 M2fycGZ2dmO2aX;uJIF{e2G7 NHTjNVkyKHSxIEGwJJVO NGe2d5QzPCCqcoO= NFu3[FhKdmirYnn0bY9vKG:oIG\QV|M1KGmwIHj1cYFvKESOREGgZ4VtdHNiYYPz[ZN{\WRiYYOgdJJmfmWwdHnvckBw\iCQQmKxJJBzd3SnaX6g[IVoemGmYYTpc44h[XRiMTD0c{AyOCC3TTDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= NXTOcWVrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
DLD1 NVfaXFRxTnWwY4Tpc44h[XO|YYm= NVX5XZg6OSC2bzCxNEB2VQ>? MUmyOEBpenN? NVPyepoyUW6qaXLpeIlwdiCxZjDWVHM{PCCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJJBz\X[nboTpc44hd2ZiTlTQOVIheHKxdHXpckBl\We{YXTheIlwdiCjdDCxJJRwKDFyIIXNJIlv[3WkYYTl[EBnd3JiMkSgbJJ{KGK7IGfld5Rmem5iYnzveEBidmGueYPpdy=> Moj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
DLD1 MlXOSpVv[3Srb36gZZN{[Xl? NUTHUJdQOSC2bzCxNEB2VQ>? M3zBbVI1KGi{cx?= NULMend4UW6qaXLpeIlwdiCxZjDWVHM{PCCrbjDoeY1idiCGTFSxJINmdGy|IHHzd4V{e2WmIHHzJJBz\X[nboTpc44hd2ZiRmTINUBxem:2ZXnuJIRm\3KjZHH0bY9vKGG2IEGgeI8hOTBidV2gbY5kfWKjdHXkJIZweiB{NDDodpMh[nliV3XzeIVzdiCkbH;0JIFv[Wy7c3nz M1HJNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
A2780 NV;3PVhIS3m2b4TvfIlkcXS7IHHzd4F6 MX:3NkBpenN? NVi0UXJQS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjR7zszN Mom1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
A2780 NGHKXpdHfW6ldHnvckBie3OjeR?= M33iWFE5KGi{cx?= M{DvUGlvcGmkaYTpc44hd2ZiSFTBR{BqdiCqdX3hckBCOjd6MDDj[YxteyCjZoTldkAyQCCqcoOgZpkh\my3b4Lld4NmdmOnIHHzd4F6NCCLQ{WwQVAvPjYQvF2= NU\HVmxNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
KYSE-510 NFj4RXVHfW6ldHnvckBie3OjeR?= M{[wclE5KGi{cx?= MVzJcohq[mm2aX;uJI9nKEiGQVOgbY4h[2m|cHzheIlvKHOnboPpeIl3\SCqdX3hckBMYVOHLUWxNEBk\WyuczDh[pRmeiBzODDodpMh[nliZnz1c5Jme2OnbnPlJIF{e2G7LDDJR|UxRTIQvF2= NYLlO40{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
CAL27 MojuR5l1d3SxeHnjbZR6KGG|c3H5 MnHwO|IhcHK| NWLjT4ROS3m2b4TvfIlkcXS7IHHnZYlve3RiY3nzdIxifGmwIIPlcpNqfGm4ZTDoeY1idiCFQVyyO{Bk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTFwMEROwG0> NHiwe|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
MDA-MB-231 NUL1[W5WS3m2b4TvfIlkcXS7IHHzd4F6 M161NVczKGi{cx?= NE[5N3hEgXSxdH;4bYNqfHliYXfhbY5{fCClaYPwcIF1cW5ic3Xud4l1cX[nIHj1cYFvKE2GQT3NRk0zOzFiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xMlM4|ryP M4PLNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
MDA-MB-231 NVvwRVA3S3m2b4TvfIlkcXS7IHHzd4F6 MlLSO|IhcHK| M3GxUWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGOrc4DsZZRqdiC{ZYPpd5RidnRiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGuOljPxE1? M4fpVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
CAL27 NVfxVoZtS3m2b4TvfIlkcXS7IHHzd4F6 MV:3NkBpenN? M{\uTWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGOrc4DsZZRqdiC{ZYPpd5RidnRiaIXtZY4hS0GOMkegZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0yNjhzzszN Mn;5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
KYSE-510 MlTKR5l1d3SxeHnjbZR6KGG|c3H5 MYS3NkBpenN? MWjDfZRwfG:6aXPpeJkh[WejaX7zeEBkcXOybHH0bY4hemW|aYP0ZY51KGi3bXHuJGt[W0VvNUGwJINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:Oi52ON88US=> NEWyfZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
KYSE-510 MoXOR5l1d3SxeHnjbZR6KGG|c3H5 Mly4O|IhcHK| NHPIV5FEgXSxdH;4bYNqfHliYXfhbY5{fCClaYPwcIF1cW5ic3Xud4l1cX[nIHj1cYFvKEu\U1WtOVExKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mj65Ou69VQ>? MkXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
HepG2 NUS4cpZNSW62aX3hcIFzcWGuIHHzd4F6 MkP6OFghcHK| NYPwNGgySW62aX3hcIFzcWGuIHHjeIl3cXS7IHHnZYlve3RiZYjvMYVzgXSqcn;jfZRq[yCob4LtJI9nKFCuYYPtc4RqfW1iYnXy[4hmcSCrbn\lZ5Rm\CCrbjDoeY1idiCKZYDHNkBk\WyuczDh[pRmeiB2ODDodpMtKEmFNUC9NE4yPs7:TR?= MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyNEm2O{c,OjR7MES5Olc9N2F-
HepG2 NHX1ellEgXSxdH;4bYNqfHliYYPzZZk> NF\PR4k1QCCqcoO= NX\GR5BRS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPDhiaILzMEBKSzVyPUGuNlbPxE1? NH7VXnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwOFk3Pyd-MkS5NFQ6Pjd:L3G+

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:

[1]

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HDAC IC50 Profiling:

The in vitro inhibitory activity of compounds against seven human HDAC isoforms (1, 2, 4 C2A, 5 C2A, 6, 8, and 11) are performed with a fluorescent based assay according to the company’s standard operating procedure. The IC50 values are determined using 10 different concentrations with 3-fold serial dilution starting at 10 μM. TSA and vorinostat are used as reference compounds.
Cell Research:

[1]

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  • Cell lines: A2780, Cal27, Kyse510, and MDA-MB-231 cell lines
  • Concentrations: ~10 μM
  • Incubation Time: 72 hours
  • Method:

    The rate of cell survival under the action of test substances is evaluated by an improved MTT assay. The assay is based on the ability of viable cells to metabolize yellow MTT to violet formazan that can be detected spectrophotometrically. In brief, A2780, Cal27, Kyse510, and MDA-MB-231 cell lines are seeded at a density of 5000, 7000, 8000, and 10 000 cells/well in 96-well plates. After 24 h, cells are exposed to increased concentrations of the test compounds. Incubation is ended after 72 h, and cell survival is determined by addition of MTT solution (5 mg/mL in phosphate buffered saline). The formazan precipitate is dissolved in DMSO. Absorbance s measured at 544 and 690 nm in a FLUOstar microplate reader.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (197.04 mM)
Ethanol 58 mg/mL (197.04 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+30%PEG300+5%TW80+ddH2O
For best results, use promptly after mixing.
13mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 294.35
Formula

C15H22N2O4

CAS No. 1418033-25-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CC(=CC(=C1)C(=O)NOCCCCCC(=O)NO)C

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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HDAC Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID