For research use only.

Catalog No.S7569

20 publications

LMK-235 Chemical Structure

CAS No. 1418033-25-6

LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.

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Selleck's LMK-235 has been cited by 20 publications

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Biological Activity

Description LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.
HDAC5 [1]
(Cell-free assay)
HDAC4 [1]
(Cell-free assay)
4.2 nM 11.9 nM
In vitro

LMK-235 causes HDAC inhibition with IC50 of <1 μM in human cancer cell lines with different sensitivity towards cisplatin. In breast cancer cell line MDA-MB-231, tongue cancer cell line Cal27, and esophagus cell line Kyse510 cell line, LMK-235 displays a high cytotoxicity, and markedly enhances the cytotoxicity of cisplatin. [1] In addition, LMK-235 also shows nanomolar activity against multiple malaria parasite life cycle stages. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human A2780 cells MkH5R5l1d3SxeHnjbZR6KGG|c3H5 MkftO|IhcA>? NWW5cZZuS3m2b4TvfIlkcXS7IHHnZYlve3RiY3nzdIxifGmwIILld4l{fGGwdDDoeY1idiCDMke4NEBk\WyuczDh[pRmeiB5MjDodpMh[nliTWTUJIF{e2G7LDDJR|UxRTBwM{Kg{txO MmrFNlMzPTJ4MEO=
human A2780 cells NHTOVmdEgXSxdH;4bYNqfHliYYPzZZk> MWe3NkBp MV3DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBNlc5OCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUCuOFkh|ryP M2W1flI{OjV{NkCz
human A2780 cells MVvDfZRwfG:6aXPpeJkh[XO|YYm= MYmxPEBp NGm4NZFKdmirYnn0bY9vKG:oIFjERWMhcW5iaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhOThiaILzJIJ6KG[udX;y[ZNk\W6lZTDhd5Nige,:jDDJR|UxRTBwNkWg{txO MVSyN|I2OjZyMx?=
human HepG2 cells NHr6RpNEgXSxdH;4bYNqfHliYYPzZZk> Mn[4OFghcA>? NYrqdphJS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hUGWyR{KgZ4VtdHNiYX\0[ZIhPDhiaILzMEBKSzZyPUGuNlYh|ryP NXXxUHNmOjR7MES5Olc>
human MDA-MB-231 cells NHLycZNEgXSxdH;4bYNqfHliYYPzZZk> MkO1O|IhcA>? MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBkcXOybHH0bY4he2Wwc3n0bZZmKGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zLkO3JO69VQ>? M2LuRVI{OjV{NkCz
human KYSE-510 cells MV\DfZRwfG:6aXPpeJkh[XO|YYm= MWG3NkBp NW\HW2pQS3m2b4TvfIlkcXS7IHHnZYlve3RiY3nzdIxifGmwIILld4l{fGGwdDDoeY1idiCNWWPFMVUyOCClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUKuOFgh|ryP NHjtZY0zOzJ3Mk[wNy=>

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Kinase Assay:


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HDAC IC50 Profiling:

The in vitro inhibitory activity of compounds against seven human HDAC isoforms (1, 2, 4 C2A, 5 C2A, 6, 8, and 11) are performed with a fluorescent based assay according to the company’s standard operating procedure. The IC50 values are determined using 10 different concentrations with 3-fold serial dilution starting at 10 μM. TSA and vorinostat are used as reference compounds.
Cell Research:


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  • Cell lines: A2780, Cal27, Kyse510, and MDA-MB-231 cell lines
  • Concentrations: ~10 μM
  • Incubation Time: 72 hours
  • Method:

    The rate of cell survival under the action of test substances is evaluated by an improved MTT assay. The assay is based on the ability of viable cells to metabolize yellow MTT to violet formazan that can be detected spectrophotometrically. In brief, A2780, Cal27, Kyse510, and MDA-MB-231 cell lines are seeded at a density of 5000, 7000, 8000, and 10 000 cells/well in 96-well plates. After 24 h, cells are exposed to increased concentrations of the test compounds. Incubation is ended after 72 h, and cell survival is determined by addition of MTT solution (5 mg/mL in phosphate buffered saline). The formazan precipitate is dissolved in DMSO. Absorbance s measured at 544 and 690 nm in a FLUOstar microplate reader.

    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (197.04 mM)
Ethanol 58 mg/mL (197.04 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
For best results, use promptly after mixing.

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 294.35


CAS No. 1418033-25-6
Storage powder
in solvent
Synonyms N/A

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID