LMK-235

For research use only.

Catalog No.S7569

22 publications

LMK-235 Chemical Structure

CAS No. 1418033-25-6

LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.

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Selleck's LMK-235 has been cited by 22 publications

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Choose Selective HDAC Inhibitors

Biological Activity

Description LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.
Targets
HDAC5 [1]
(Cell-free assay)
HDAC4 [1]
(Cell-free assay)
4.2 nM 11.9 nM
In vitro

LMK-235 causes HDAC inhibition with IC50 of <1 μM in human cancer cell lines with different sensitivity towards cisplatin. In breast cancer cell line MDA-MB-231, tongue cancer cell line Cal27, and esophagus cell line Kyse510 cell line, LMK-235 displays a high cytotoxicity, and markedly enhances the cytotoxicity of cisplatin. [1] In addition, LMK-235 also shows nanomolar activity against multiple malaria parasite life cycle stages. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A2780 NFPDWGdEgXSxdH;4bYNqfHliYYPzZZk> NUXqTotnPzJiaILz M{nqRWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGOrc4DsZZRqdiC{ZYPpd5RidnRiaIXtZY4hSTJ5OECgZ4VtdHNiYX\0[ZIhPzJiaILzJIJ6KE2WVDDhd5NigSxiSVO1NF0xNjN{zszN NEjsdlM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
A2780 M4fJdWZ2dmO2aX;uJIF{e2G7 NFjOfWgyQCCqcoO= M{jRT2lvcGmkaYTpc44hd2ZiSFTBR{BqdiClaYPwcIF1cW5icnXzbZN1[W62IHj1cYFvKEF{N{iwJINmdGy|IHHmeIVzKDF6IHjyd{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVA:OC5|Mt88US=> MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
DLD1 Mlu2SpVv[3Srb36gZZN{[Xl? M4TaV|EhfG9iMUCgeW0> NGLUVFgzPCCqcoO= MoL1TY5pcWKrdHnvckBw\iCYUGOzOEBqdiCqdX3hckBFVERzIHPlcIx{KGG|c3Xzd4VlKGG|IIDy[ZZmdnSrb36gc4YhVkORQUSgdJJwfGWrbjDk[Ydz[WSjdHnvckBifCBzIITvJFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
DLD1 NIPlU4tHfW6ldHnvckBie3OjeR?= MkXoNUB1dyBzMDD1US=> NY[3RoFmOjRiaILz MYnJcohq[mm2aX;uJI9nKF[SU{O0JIlvKGi3bXHuJGRNTDFiY3XscJMh[XO|ZYPz[YQh[XNicILleoVvfGmxbjDv[kBxPjJiZHXndoFl[XSrb36gZZQhOSC2bzCxNEB2VSCrbnP1ZoF1\WRiZn;yJFI1KGi{czDifUBY\XO2ZYLuJIJtd3RiYX7hcJl{cXN? Mni5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
DLD1 MnzESpVv[3Srb36gZZN{[Xl? M1\LWFEhfG9iMUCgeW0> NVLkdm5yOjRiaILz NGC0PW1KdmirYnn0bY9vKG:oIG\QV|M1KGmwIHj1cYFvKESOREGgZ4VtdHNiYYPz[ZN{\WRiYYOgdJJmfmWwdHnvckBw\iCQQmKxJJBzd3SnaX6g[IVoemGmYYTpc44h[XRiMTD0c{AyOCC3TTDpcoN2[mG2ZXSg[o9zKDJ2IHjyd{BjgSCZZYP0[ZJvKGKub4SgZY5idHm|aYO= M3K0NFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
DLD1 MVTGeY5kfGmxbjDhd5NigQ>? NH3oUFgyKHSxIEGwJJVO MnjGNlQhcHK| MnLxTY5pcWKrdHnvckBw\iCYUGOzOEBqdiCqdX3hckBFVERzIHPlcIx{KGG|c3Xzd4VlKGG|IIDy[ZZmdnSrb36gc4YhVkSSNUKgdJJwfGWrbjDk[Ydz[WSjdHnvckBifCBzIITvJFExKHWPIHnuZ5Vj[XSnZDDmc5IhOjRiaILzJIJ6KFenc4Tldo4h[myxdDDhcoFtgXOrcx?= M4WzcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
DLD1 MmrPSpVv[3Srb36gZZN{[Xl? MoX2NUB1dyBzMDD1US=> M3i4TVI1KGi{cx?= MnrJTY5pcWKrdHnvckBw\iCYUGOzOEBqdiCqdX3hckBFVERzIHPlcIx{KGG|c3Xzd4VlKGG|IIDy[ZZmdnSrb36gc4YhTlSKMTDwdo91\WmwIHTl[5Ji\GG2aX;uJIF1KDFidH:gNVAhfU1iaX7jeYJifGWmIH\vdkAzPCCqcoOgZpkhX2W|dHXyckBjdG:2IHHuZYx6e2m| NFi0UII9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
A2780 M4LmfWN6fG:2b4jpZ4l1gSCjc4PhfS=> NX\3UGNpPzJiaILz Ml;XR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVI4QDBiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2wMlQ6|ryP M1HpO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
A2780 NW\Fc3VWTnWwY4Tpc44h[XO|YYm= NUS0eGhlOThiaILz NYfvNWRJUW6qaXLpeIlwdiCxZjDISGFEKGmwIHj1cYFvKEF{N{iwJINmdGy|IHHmeIVzKDF6IHjyd{BjgSCobIXvdoV{[2WwY3WgZZN{[XluIFnDOVA:OC54Nd88US=> MX68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
KYSE-510 NH20NldHfW6ldHnvckBie3OjeR?= MYOxPEBpenN? NYP0eJo5UW6qaXLpeIlwdiCxZjDISGFEKGmwIHPpd5Bt[XSrbjDz[Y5{cXSrdnWgbJVu[W5iS2nTSU02OTBiY3XscJMh[W[2ZYKgNVghcHK|IHL5JIZtfW:{ZYPj[Y5k\SCjc4PhfUwhUUN3ME2x{txO NViw[IkxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
CAL27 MVXDfZRwfG:6aXPpeJkh[XO|YYm= MWi3NkBpenN? NE\KRoFEgXSxdH;4bYNqfHliYXfhbY5{fCClaYPwcIF1cW5ic3Xud4l1cX[nIHj1cYFvKEODTEK3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS5yM988US=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
MDA-MB-231 MkDtR5l1d3SxeHnjbZR6KGG|c3H5 MU[3NkBpenN? MmTSR5l1d3SxeHnjbZR6KGGpYXnud5Qh[2m|cHzheIlvKHOnboPpeIl3\SCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;MT6zO:69VQ>? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
MDA-MB-231 MlrSR5l1d3SxeHnjbZR6KGG|c3H5 NFXCdo84OiCqcoO= M{PxVGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGOrc4DsZZRqdiC{ZYPpd5RidnRiaIXtZY4hVUSDLV3CMVI{OSClZXzsd{Bi\nSncjC3NkBpenNiYomgUXRVKGG|c3H5MEBKSzVyPUGuOljPxE1? NX\vO5M2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
CAL27 NFzDXWdEgXSxdH;4bYNqfHliYYPzZZk> Mnq1O|IhcHK| NHj1fJlEgXSxdH;4bYNqfHliYXfhbY5{fCClaYPwcIF1cW5icnXzbZN1[W62IHj1cYFvKEODTEK3JINmdGy|IHHmeIVzKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS56Md88US=> MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
KYSE-510 MWHDfZRwfG:6aXPpeJkh[XO|YYm= NX;ZSHlMPzJiaILz NEnjcmNEgXSxdH;4bYNqfHliYXfhbY5{fCClaYPwcIF1cW5icnXzbZN1[W62IHj1cYFvKEu\U1WtOVExKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDNWHQh[XO|YYmsJGlEPTB;Mj60PO69VQ>? M1W5OVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
KYSE-510 MYfDfZRwfG:6aXPpeJkh[XO|YYm= NXr4TYxTPzJiaILz M1iyNmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGOrc4DsZZRqdiC|ZX7zbZRqfmViaIXtZY4hU1mVRT21NVAh[2WubIOgZYZ1\XJiN{KgbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1{Lkm2{txO MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
HepG2 MWfBcpRqdWGuYYLpZYwh[XO|YYm= M1;JO|Q5KGi{cx?= MnizRY51cW2jbHHybYFtKGGldHn2bZR6KGGpYXnud5Qh\XixLXXyfZRpem:leYTpZ{Bnd3KvIH;mJHBt[XOvb3TpeY0h[mW{Z3jlbUBqdm[nY4Tl[EBqdiCqdX3hckBJ\XCJMjDj[YxteyCjZoTldkA1QCCqcoOsJGlEPTB;MD6xOu69VQ>? NGW3WG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwOFk3Pyd-MkS5NFQ6Pjd:L3G+
HepG2 NWLofllDS3m2b4TvfIlkcXS7IHHzd4F6 M3X3eVQ5KGi{cx?= Mo\YR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVxTzJiY3XscJMh[W[2ZYKgOFghcHK|LDDJR|UxRTFwMkdOwG0> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyNEm2O{c,OjR7MES5Olc9N2F-

... Click to View More Cell Line Experimental Data

Protocol

Kinase Assay:

[1]

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HDAC IC50 Profiling:

The in vitro inhibitory activity of compounds against seven human HDAC isoforms (1, 2, 4 C2A, 5 C2A, 6, 8, and 11) are performed with a fluorescent based assay according to the company’s standard operating procedure. The IC50 values are determined using 10 different concentrations with 3-fold serial dilution starting at 10 μM. TSA and vorinostat are used as reference compounds.
Cell Research:

[1]

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  • Cell lines: A2780, Cal27, Kyse510, and MDA-MB-231 cell lines
  • Concentrations: ~10 μM
  • Incubation Time: 72 hours
  • Method:

    The rate of cell survival under the action of test substances is evaluated by an improved MTT assay. The assay is based on the ability of viable cells to metabolize yellow MTT to violet formazan that can be detected spectrophotometrically. In brief, A2780, Cal27, Kyse510, and MDA-MB-231 cell lines are seeded at a density of 5000, 7000, 8000, and 10 000 cells/well in 96-well plates. After 24 h, cells are exposed to increased concentrations of the test compounds. Incubation is ended after 72 h, and cell survival is determined by addition of MTT solution (5 mg/mL in phosphate buffered saline). The formazan precipitate is dissolved in DMSO. Absorbance s measured at 544 and 690 nm in a FLUOstar microplate reader.


    (Only for Reference)

Solubility (25°C)

In vitro DMSO 58 mg/mL (197.04 mM)
Ethanol 58 mg/mL (197.04 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+30%PEG300+5%TW80+ddH2O
For best results, use promptly after mixing.
13mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 294.35
Formula

C15H22N2O4

CAS No. 1418033-25-6
Storage powder
in solvent
Synonyms N/A
Smiles CC1=CC(=CC(=C1)C(=O)NOCCCCCC(=O)NO)C

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HDAC Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID