Enoxacin

Catalog No.S1756 Synonyms: AT-2266, CI919, Pd107779, NSC 629661

For research use only.

Enoxacin (AT-2266, CI919, Pd107779, NSC 629661) is an oral broad-spectrum fluoroquinolone antibacterial agent by inhibiting bacterial DNA gyrase and topoisomerase IV, used to treat a wide variety of infections.

Enoxacin  Chemical Structure

CAS No. 74011-58-8

Selleck's Enoxacin has been cited by 1 Publication

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Biological Activity

Description Enoxacin (AT-2266, CI919, Pd107779, NSC 629661) is an oral broad-spectrum fluoroquinolone antibacterial agent by inhibiting bacterial DNA gyrase and topoisomerase IV, used to treat a wide variety of infections.
Targets
Topoisomerase II [1] Topoisomerase IV [1]
In vitro

Enoxacin, a fluoroquinolone used as an antibacterial compound, enhances the production of miRNAs with tumor suppressor functions by binding to the miRNA biosynthesis protein TAR RNA-binding protein 2 (TRBP). [1] Enoxacin binds to the DNA active site and alters the breakage/reunion activity of the enzyme. Enoxacin stimulates cleavage of both relaxed and supercoiled forms of DNA in the absence of ATP, whereas CcdB induces cleavage only after many cycles of ATP-dependent breakage and reunion. [2] Enoxacin dose dependently reduces the number of osteoclasts differentiating in mouse marrow cultures stimulated with 1,25-dihydroxyvitamin D(3), as well as markers of osteoclast activity, and the number of resorption lacunae formed on bone slices. Enoxacin inhibits osteoclast formation at concentrations where osteoblast formation is not altered. [3] Enoxacin dose-dependently reduces the number of multinuclear cells expressing tartrate-resistant acid phosphatase (TRAP) activity produced by RANK-L-stimulated osteoclast precursors. Enoxacin directly inhibits osteoclast formation without affecting cell viability by a novel mechanism that involves changes in posttranslational processing and trafficking of several proteins with known roles in osteoclast function. [4] Enoxacin is able to decrease cell viability, induce apoptosis, cause cell cycle arrest, and inhibit the invasiveness of prostate cancer (PCa) cell lines. Enoxacin is also effective in restoring the global expression of miRNAs in prostate cancer (PCa) cell lines. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 320.32
Formula

C15H17FN4O3

CAS No. 74011-58-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCN1C=C(C(=O)C2=CC(=C(N=C21)N3CCNCC3)F)C(=O)O

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04840823 Recruiting Drug: Enoxacin|Drug: Placebo Amyotrophic Lateral Sclerosis McGill University|Weizmann Institute of Science|Apotex Inc. March 26 2021 Phase 1|Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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