CRISPR/Cas9

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CRISPR/Cas9 Products

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  • CRISPR/Cas9 Inhibitors (2)
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  • New CRISPR/Cas9 Products
Catalog No. Product Name Information Product Use Citations Product Validations
S0102 L 189 L 189 is a novel human DNA ligase inhibitor with IC50 of 5 μM, 9 μM and 5 μM for hLigI, hLigIII and hLigIV, respectively. L 189 inhibits base excision repair (BER) and non-homologous end joining (NHEJ). L 189 specifically sensitizes cancer cells to DNA damage.
S8844 BRD0539 BRD0539 is a potent inhibitor of Streptococcus pyogenes Cas9 (SpCas9) with IC50 of 22 μM in an in vitro DNA cleavage assay.
Mol Cell, 2021, S1097-2765(21)00168-4
S2638 NU7441 (KU-57788) NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits mTOR and PI3K with IC50 of 1.7 μM and 5 μM in cell-free assays, respectively. It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage.
Cell Metab, 2022, S1550-4131(21)00542-8
Immunity, 2021, S1074-7613(21)00069-8
Cell Res, 2021, 10.1038/s41422-021-00528-3
S2775 Nocodazole (R17934) Nocodazole (R17934, Oncodazole, NSC238159) is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole induces apoptosis.
Mol Cancer Res, 2022, molcanres.0612.2021
Cancer Cell, 2021, S1535-6108(21)00383-4
Nat Immunol, 2021, 10.1038/s41590-021-01090-1
S7046 Brefeldin A Brefeldin A is a lactone antibiotic and ATPase inhibitor for protein transport with IC50 of 0.2 μM in HCT 116 cells, induces cancer cell differentiation and apoptosis. It could also improve the HDR(homology-directed repair) efficiency and be an enhancer of CRISPR-mediated HDR. Brefeldin A is also an inhibitor of autophagy and mitophagy.
Nat Cell Biol, 2021, 10.1038/s41556-021-00704-y
Nat Biomed Eng, 2021, 10.1038/s41551-020-00675-9
Cell Rep, 2021, 36(6):109516
S7742 SCR7 SCR7 is a specific DNA Ligase IV inhibitor, which blocks nonhomologous end-joining (NHEJ). It increases the efficiency of HDR-mediated genome editing up to 19-fold using CRISPR/Cas9 in mammalian cells and mouse embryos.
Protein Cell, 2021, 10.1007/s13238-021-00879-y 10.1126/science.abc5386
Pharmacol Res, 2021, S1043-6618(21)00518-1
Cold Spring Harb Protoc, 2021, 10.1101/pdb.prot106989
S7827 4-Hydroxytamoxifen (Afimoxifene) 4-Hydroxytamoxifen (Afimoxifene) is the active metabolite of tamoxifen and a selective estrogen receptor (ER) modulator that is widely used in the therapeutic and chemopreventive treatment of breast cancer. It activates on-target modification frequency for intein-Cas9 variants.
Sci Adv, 2021, 7(43):eabh1022
Nat Commun, 2021, 12(1):6020
BMC Cancer, 2021, 21(1):931
S7974 L755507 L-755,507 is characterized as a potent and selective β3 adrenergic receptor partial agonist with EC50 of 0.43 nM. It is also recently identified to enhance CRISPR-mediated homology-directed repair (HDR) efficiency in human induced pluripotent stem cells (iPSCs) and other cell types.
Hum Mutat, 2019, 10.1002/humu.23848
S8234 RS-1 RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer that enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.
Int J Biol Sci, 2021, 17(4):1026-1040
S2579 Zidovudine Zidovudine (ZDV, Azidothymidine, NSC 602670) is a nucleoside analogue reverse transcriptase inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.
J Virol, 2022, JVI0173021
Biochem Pharmacol, 2021, 197:114901
PLoS Pathog, 2020, 16;16(3):e1008341
S0102 L 189 L 189 is a novel human DNA ligase inhibitor with IC50 of 5 μM, 9 μM and 5 μM for hLigI, hLigIII and hLigIV, respectively. L 189 inhibits base excision repair (BER) and non-homologous end joining (NHEJ). L 189 specifically sensitizes cancer cells to DNA damage.
S8844 BRD0539 BRD0539 is a potent inhibitor of Streptococcus pyogenes Cas9 (SpCas9) with IC50 of 22 μM in an in vitro DNA cleavage assay.
Mol Cell, 2021, S1097-2765(21)00168-4
S2638 NU7441 (KU-57788) NU7441 (KU-57788) is a highly potent and selective DNA-PK inhibitor with IC50 of 14 nM and also inhibits mTOR and PI3K with IC50 of 1.7 μM and 5 μM in cell-free assays, respectively. It reduces the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage.
Cell Metab, 2022, S1550-4131(21)00542-8
Immunity, 2021, S1074-7613(21)00069-8
Cell Res, 2021, 10.1038/s41422-021-00528-3
S2775 Nocodazole (R17934) Nocodazole (R17934, Oncodazole, NSC238159) is a rapidly-reversible inhibitor of microtubule polymerization, also inhibits Abl, Abl(E255K) and Abl(T315I) with IC50 of 0.21 μM, 0.53 μM and 0.64 μM in cell-free assays, respectively. Nocodazole induces apoptosis.
Mol Cancer Res, 2022, molcanres.0612.2021
Cancer Cell, 2021, S1535-6108(21)00383-4
Nat Immunol, 2021, 10.1038/s41590-021-01090-1
S7046 Brefeldin A Brefeldin A is a lactone antibiotic and ATPase inhibitor for protein transport with IC50 of 0.2 μM in HCT 116 cells, induces cancer cell differentiation and apoptosis. It could also improve the HDR(homology-directed repair) efficiency and be an enhancer of CRISPR-mediated HDR. Brefeldin A is also an inhibitor of autophagy and mitophagy.
Nat Cell Biol, 2021, 10.1038/s41556-021-00704-y
Nat Biomed Eng, 2021, 10.1038/s41551-020-00675-9
Cell Rep, 2021, 36(6):109516
S7742 SCR7 SCR7 is a specific DNA Ligase IV inhibitor, which blocks nonhomologous end-joining (NHEJ). It increases the efficiency of HDR-mediated genome editing up to 19-fold using CRISPR/Cas9 in mammalian cells and mouse embryos.
Protein Cell, 2021, 10.1007/s13238-021-00879-y 10.1126/science.abc5386
Pharmacol Res, 2021, S1043-6618(21)00518-1
Cold Spring Harb Protoc, 2021, 10.1101/pdb.prot106989
S7827 4-Hydroxytamoxifen (Afimoxifene) 4-Hydroxytamoxifen (Afimoxifene) is the active metabolite of tamoxifen and a selective estrogen receptor (ER) modulator that is widely used in the therapeutic and chemopreventive treatment of breast cancer. It activates on-target modification frequency for intein-Cas9 variants.
Sci Adv, 2021, 7(43):eabh1022
Nat Commun, 2021, 12(1):6020
BMC Cancer, 2021, 21(1):931
S7974 L755507 L-755,507 is characterized as a potent and selective β3 adrenergic receptor partial agonist with EC50 of 0.43 nM. It is also recently identified to enhance CRISPR-mediated homology-directed repair (HDR) efficiency in human induced pluripotent stem cells (iPSCs) and other cell types.
Hum Mutat, 2019, 10.1002/humu.23848
S8234 RS-1 RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer that enhances Cas9- and TALEN-mediated knock-in efficiency in rabbit embryos both in vitro and in vivo.
Int J Biol Sci, 2021, 17(4):1026-1040
S2579 Zidovudine Zidovudine (ZDV, Azidothymidine, NSC 602670) is a nucleoside analogue reverse transcriptase inhibitor, used to treat HIV. It could decrease the HDR efficiency and decrease CRISPR-mediated sequence-specific genome knockin events while increaseing knockout efficiency.
J Virol, 2022, JVI0173021
Biochem Pharmacol, 2021, 197:114901
PLoS Pathog, 2020, 16;16(3):e1008341
Tags: CRISPR/Cas9 enhancer|CRISPR/Cas9 activator|CRISPR/Cas9 inhibitor