For research use only.

Catalog No.S2423 Synonyms: 10-HCPT

3 publications

(S)-10-Hydroxycamptothecin Chemical Structure

CAS No. 19685-09-7

(S)-10-Hydroxycamptothecin (10-HCPT) is a DNA topoisomerase I inhibitor with potent anti-tumor activity.

Selleck's (S)-10-Hydroxycamptothecin has been cited by 3 publications

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Biological Activity

Description (S)-10-Hydroxycamptothecin (10-HCPT) is a DNA topoisomerase I inhibitor with potent anti-tumor activity.
DNA topoisomerase I [1]
In vitro

10-Hydroxycamptothecin inhibits the growth of BT-20 and MDA-231 cell with IC50 of 34.3 nM and 7.27 nM, respectively, more potently than camptothecin (CPT) with IC50 of >500 nM. 10-Hydroxycamptothecin potently induces human topoisomerase I-mediated cleavable complex formation of pBR322 plasmid DNA with EC50 of 0.35 μM, displaying >50-fold potency than CPT with EC50 of 18.85 μM. [2] 10-Hydroxycamptothecin treatment induces dose-dependent growth inhibition of human microvascular endothelial cells (HMEC) with IC50 of 0.31 μM, and significantly inhibits the migration of HMEC with IC50 of 0.63 μM. Treatment of HMEC cells with 10-Hydroxycamptothecin also results in a dose-dependent inhibition of tube formation with IC50 of 0.96 μM. [3] 10-Hydroxycamptothecin (5-20 nM) significantly inhibits the proliferation of Colo 205 cells, arrests the cells in the G2 phase of the cell cycle and induces apoptosis through a caspase-3-dependent pathway. [4]

In vivo 10-Hydroxycamptothecin treatment inhibits angiogenesis in a concentration-dependent fashion in the CAM model, with 95% inhibition at dose of 25 nM, more potently than suramin which at 125 nM inhibits angiogenesis only by 60%. [3] Oral administration of 10-Hydroxycamptothecin at low doses of 2.5-7.5 mg/kg every 2 days leads to significant growth inhibition of Colo 205 xenografts in mice without acute toxicity. [4] LD50: Mice 104mg/kg (i.p.). [5]


Cell Research:[2]
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  • Cell lines: MDA-231, and BT-20 cells
  • Concentrations: Dissolved in DMSO, final concentrations ~50 μM
  • Incubation Time: 72 hours
  • Method: Cells are exposed to various concentrations of 10-Hydroxycamptothecin for 72 hours. Cell growth is monitored by the standard MTT assay.
    (Only for Reference)
Animal Research:[4]
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  • Animal Models: BALB/c-nu mice transplanted subcutaneously with Colo 205 cells
  • Dosages: ~7.5 mg/kg
  • Administration: Orally once per two days
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 8 mg/mL warmed (21.95 mM)
Water Insoluble
Ethanol Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
30% PEG400+0.5% Tween80+5% propylene glycol
For best results, use promptly after mixing.
30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 364.35


CAS No. 19685-09-7
Storage powder
in solvent
Synonyms 10-HCPT
Smiles CCC1(C2=C(COC1=O)C(=O)N3CC4=C(C3=C2)N=C5C=CC(=CC5=C4)O)O

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT01963182 Completed Drug: Irinotecan First Line Metastatic Colorectal Cancer Centre Jean Perrin October 2013 Phase 2
NCT00520390 Completed Drug: EZN-2208 Lymphoma|Advanced Solid Tumors Enzon Pharmaceuticals Inc. May 2007 Phase 1
NCT00520637 Completed Drug: EZN-2208 Advanced Solid Tumors|Lymphoma Enzon Pharmaceuticals Inc. May 2007 Phase 1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID