Camptothecin

For research use only.

Catalog No.S1288 Synonyms: NSC-100880, CPT, Campathecin, (S)-(+)-Camptothecin

78 publications

Camptothecin Chemical Structure

CAS No. 7689-03-4

Camptothecin (NSC-100880, CPT, Campathecin, (S)-(+)-Camptothecin) is a specific inhibitor of DNA topoisomerase I (Topo I) with IC50 of 0.68 μM in a cell-free assay. Camptothecin induces apoptosis in cancer cells via microRNA-125b-mediated mitochondrial pathways. Phase 2.

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Selleck's Camptothecin has been cited by 78 publications

Purity & Quality Control

Choose Selective ADC Cytotoxin Inhibitors

Biological Activity

Description Camptothecin (NSC-100880, CPT, Campathecin, (S)-(+)-Camptothecin) is a specific inhibitor of DNA topoisomerase I (Topo I) with IC50 of 0.68 μM in a cell-free assay. Camptothecin induces apoptosis in cancer cells via microRNA-125b-mediated mitochondrial pathways. Phase 2.
Targets
Topo I [2]
(Cell-free assay)
0.68 μM
In vitro

Camptothecin, a plant alkaloid orignially isolated from Camptotheca acuminate in 1966. [1] Camptothecin is noted to halt cells during the S phase of mitosis. Camptothecin displays nanomolar potency in cytotoxicity against many human tumor cell lines, including HT29, LOX, SKOV3, and SKVLB, with IC50 values ranging from 37 nM to 48 nM. [2] In combination with TNF, Camptothecin induces apoptosis in primary mouse hepatocytes, with an IC50 value of 13 μM. Camptothecin also abrogated the TNF-induced NF-κB Activation, as well as the expression of TNF-receptor associated factor 2 (TRAF2), X-linked inhibitor of apoptosis protein (X-IAP), and FLICE-inhibitory protein (FLIP). [4] In HCT116 cells, Camptothecin (5 μM) induces proteasome-mediated degradation of mixed lineage leukemia 5 (MLL5) protein, which leads to phosphorylation of p53 at Ser392. [5] Due to the low solubility and adverse effects of Camptothecin, various Camptothecin analogues have been developed, and two of them, topotecan and irinotecan, has been approved by FDA and are used in cancer chemotherapy.

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
P388 M{fvU2dzd3e2aDDpcohq[mm2b4L5JIF{e2G7 MWfJR|UxRTN{IH7N NXjOPXJlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCzOFY6OzNpPkGwN|Q3QTN|PD;hQi=>
P388CPT5 R M{PxU2dzd3e2aDDpcohq[mm2b4L5JIF{e2G7 Mn\LTWM2OD1{Lkig{txO NV;FU3JkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUCzOFY6OzNpPkGwN|Q3QTN|PD;hQi=>
KBwt MoT4S5Jwf3SqIHnubIljcXSxcomgZZN{[Xl? M3j1UGlEPTB;NECgcm0> Ml7GQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTB2MUG0O|YoRjFyNEGxOFc3RC:jPh?=
KBMDR NWLDdW8yT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= MVfJR|UxRTdyIH7N NFT3OoE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMESxNVQ4Pid-MUC0NVE1PzZ:L3G+
KBV20C NGmydoJIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= NUSzWGJJUUN3ME2zNEBvVQ>? NGHLc4M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMESxNVQ4Pid-MUC0NVE1PzZ:L3G+
KB7D NXnCUJR4T3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NHLOfoJKSzVyPUO1JI5O NVjL[3JXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC0NVE1PzZpPkGwOFEyPDd4PD;hQi=>
KBCamp M1\0Wmdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NXm2[og3UUN3ME2xMlA1KM7:TR?= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODRzMUS3Okc,OTB2MUG0O|Y9N2F-
HT29 MVHjfZRwfG:6aXPpeJkh[XO|YYm= NVLjW284UUN3ME24NEBvVQ>? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yODh2MUiwPEc,OTB6NEG4NFg9N2F-
A549 MmT4Z5l1d3SxeHnjbZR6KGG|c3H5 NUjwW3BkUUN3ME22O{BvVQ>? NWjiUYx2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC4OFE5ODhpPkGwPFQyQDB6PD;hQi=>
T24 MofVZ5l1d3SxeHnjbZR6KGG|c3H5 M3:yTWlEPTB;OEigcm0> NYjQfXpHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUC4OFE5ODhpPkGwPFQyQDB6PD;hQi=>
HOP-62 NF;qXXJIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M2XGSmlEPTB;MUCgcm0> NGHPZVg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUCyNFI5Oyd-MUGwNlAzQDN:L3G+
HCT-116 NVv6fWFoT3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NFvONIhKSzVyPUOwJI5O M1zUeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMEKwNlg{Lz5zMUCyNFI5OzxxYU6=
SF-539 MYfHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? MWDJR|UxRTFyIH7N M4nnNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMEKwNlg{Lz5zMUCyNFI5OzxxYU6=
UACC-62 NV36fWV4T3Kxd4ToJIlvcGmkaYTvdpkh[XO|YYm= NFjHZlRKSzVyPUGwJI5O M2TP[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMEKwNlg{Lz5zMUCyNFI5OzxxYU6=
OVCAR-3 NILDfmFIem:5dHigbY5pcWKrdH;yfUBie3OjeR?= M1[yR2lEPTB;MkKwJI5O NEHxbJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUCyNFI5Oyd-MUGwNlAzQDN:L3G+
SN12C MWfHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? NFrTPZBKSzVyPUKwJI5O M1fVXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzMEKwNlg{Lz5zMUCyNFI5OzxxYU6=
DU-145 M3PDPGdzd3e2aDDpcohq[mm2b4L5JIF{e2G7 NH:1[JJKSzVyPUGwJI5O MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTB{MEK4N{c,OTFyMkCyPFM9N2F-
MDA-MB-435 MYfHdo94fGhiaX7obYJqfG:{eTDhd5NigQ>? NH[wUI9KSzVyPUSwJI5O MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTB{MEK4N{c,OTFyMkCyPFM9N2F-
WiDr NFPqOo5kgXSxdH;4bYNqfHliYYPzZZk> MmTmSG1UVw>? NGrYcYZKSzVyPUG3JI5O MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTN|NEW2PUc,OTF|M{S1Olk9N2F-
A549 NUXsVo16[3m2b4TvfIlkcXS7IHHzd4F6 NYrQZ2w5TE2VTx?= MnXzTWM2OD1zNDDuUS=> MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOTN|NEW2PUc,OTF|M{S1Olk9N2F-
MKN45 MkX2Z5l1d3SxeHnjbZR6KGG|c3H5 NHn5UHZFVVOR MlraTWM2OD1zNzDuUS=> NEHLUZc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUOzOFU3QSd-MUGzN|Q2Pjl:L3G+
SK-OV-3 MlXnZ5l1d3SxeHnjbZR6KGG|c3H5 NXfvWHFXTE2VTx?= NUf5fYR6UUN3ME2yNEBvVQ>? NEPMd4o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUOzOFU3QSd-MUGzN|Q2Pjl:L3G+
H128 NUXpd|BI[3m2b4TvfIlkcXS7IHHzd4F6 MlPySG1UVw>? MXfJR|UxRTF6IH7N MnjZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF|M{S1OlkoRjFzM{O0OVY6RC:jPh?=
SK-BR-3 NVnyOXpD[3m2b4TvfIlkcXS7IHHzd4F6 NFzpO|BFVVOR NWWzTlU5UUN3ME2yNEBvVQ>? M1;Vc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzM{O0OVY6Lz5zMUOzOFU3QTxxYU6=
LX-1 MkPSZ5l1d3SxeHnjbZR6KGG|c3H5 NUHM[GU6TE2VTx?= NFu3VoVKSzVyPUGyNEBvVQ>? NYrM[YZDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUG4OVg4OzdpPkGxPFU5PzN5PD;hQi=>
HCT116 NVrjTY9r[3m2b4TvfIlkcXS7IHHzd4F6 NXjmRZRETE2VTx?= NXvwTpFsUUN3ME25JI5O Ml7hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTF6NUi3N|coRjFzOEW4O|M4RC:jPh?=
A2780 MoC0Z5l1d3SxeHnjbZR6KGG|c3H5 NYPDSoZlTE2VTx?= MWHJR|UxRTRibl2= NIDrZXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUi1PFc{Pyd-MUG4OVg4Ozd:L3G+
IMR-32 MlzYZ5l1d3SxeHnjbZR6KGG|c3H5 MW\+NVAh|ryP MnfTSG1UVw>? NH;1UWFKSzVyPUKuNlEhdk1? NHPpNmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[xO|g6PCd-MUK2NVc5QTR:L3G+
P388 M3r2b4N6fG:2b4jpZ4l1gSCjc4PhfS=> NELJPHRKSzVyPUGzJI5O Mn\nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ4MkCwPFEoRjF{NkKwNFgyRC:jPh?=
Lewis NIO4cY1kgXSxdH;4bYNqfHliYYPzZZk> MYXJR|UxRTN|IH7N M{Dab|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NkKwNFgyLz5zMk[yNFA5OTxxYU6=
JLC NEKzPIRkgXSxdH;4bYNqfHliYYPzZZk> MYnJR|UxRTVwNjDuUS=> MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjZ{MEC4NUc,OTJ4MkCwPFE9N2F-
HT-29 NX2wPZR7[3m2b4TvfIlkcXS7IHHzd4F6 NFvpZ|RKSzVyPUGuOEDPxE1? NUjEcZV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUK2N|k2PDFpPkGyOlM6PTRzPD;hQi=>
Caki-2 MYDjfZRwfG:6aXPpeJkh[XO|YYm= MVnJR|UxRTNwOU[g{txO MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjZ|OUW0NUc,OTJ4M{m1OFE9N2F-
A549 M3W3coN6fG:2b4jpZ4l1gSCjc4PhfS=> MmDNTWM2OD1{LkWzJO69VQ>? NEDZNJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[zPVU1OSd-MUK2N|k2PDF:L3G+
HEC-1-B MVXjfZRwfG:6aXPpeJkh[XO|YYm= NW\wVGJTUUN3ME24MlY1KM7:TR?= NFP6b5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[zPVU1OSd-MUK2N|k2PDF:L3G+
HL-60 MkPwZ5l1d3SxeHnjbZR6KGG|c3H5 M4HjWGlEPTB;Nk[gcm0> NEHlZmo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMk[zPVU1OSd-MUK2N|k2PDF:L3G+
Col2 M4\tTIN6fG:2b4jpZ4l1gSCjc4PhfS=> NVW0cVdZhjFizszN NHHxbmRGTDVyPUW3JI5O M1:4RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MESzOFA4Lz5zNUC0N|QxPzxxYU6=
HUVEC MlXoZ5l1d3SxeHnjbZR6KGG|c3H5 NVnXTGE{hjFizszN NHjIbmtGTDVyPUK1PEBvVQ>? M3vne|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MESzOFA4Lz5zNUC0N|QxPzxxYU6=
KB M3vKeoN6fG:2b4jpZ4l1gSCjc4PhfS=> MWn+NUDPxE1? NFjHNJNGTDVyPUKyJI5O NY\FPWJ{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWwOFM1ODdpPkG1NFQ{PDB5PD;hQi=>
LCNaP NGD1TZdkgXSxdH;4bYNqfHliYYPzZZk> MXn+NUDPxE1? NGfBSYFGTDVyPUK4JI5O M4\uPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3MESzOFA4Lz5zNUC0N|QxPzxxYU6=
Lu1 NF;aWG1kgXSxdH;4bYNqfHliYYPzZZk> NF75bFR,OSEQvF2= NEPocWdGTDVyPUK5JI5O NV\zVYtPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUWwOFM1ODdpPkG1NFQ{PDB5PD;hQi=>
RPMI8402 M4TSSYN6fG:2b4jpZ4l1gSCjc4PhfS=> MkjLglExKM7:TR?= MmnjTWM2OD14IH7N NVzVNHZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW0PFI6OjlpPkG1OFgzQTJ7PD;hQi=>
CPT-K5 M1HXUIN6fG:2b4jpZ4l1gSCjc4PhfS=> NEnzVoJ,OTBizszN MUnJR|UxRjFyIN88US=> Ml\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV2OEK5NlkoRjF3NEiyPVI6RC:jPh?=
P388 MYDjfZRwfG:6aXPpeJkh[XO|YYm= NX3kSoxOhjFyIN88US=> NFvtdJlKSzVyPUG0JI5O M3zPTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NEiyPVI6Lz5zNUS4NlkzQTxxYU6=
P388/CPT45 NFLa[oNkgXSxdH;4bYNqfHliYYPzZZk> NGHiVnJ,OTBizszN MVTJR|UxRjFyIN88US=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR6MkmyPUc,OTV2OEK5Nlk9N2F-
KB3-1 NH;1OGVkgXSxdH;4bYNqfHliYYPzZZk> MmPsglExKM7:TR?= NVjEbFRwUUN3ME20NEBvVQ>? MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTR6MkmyPUc,OTV2OEK5Nlk9N2F-
KBV-1 + MDR1 NFfWNHRkgXSxdH;4bYNqfHliYYPzZZk> MnvTglExKM7:TR?= NH75ZYhKSzVyPUS0NEBvVQ>? NIPTOVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS4NlkzQSd-MUW0PFI6Ojl:L3G+
KBH MnfLZ5l1d3SxeHnjbZR6KGG|c3H5 MWH+NVAh|ryP M{jlNmlEPTB;NESwJI5O NF3TVo49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUS4NlkzQSd-MUW0PFI6Ojl:L3G+
HOP-62 M4rGXoN6fG:2b4jpZ4l1gSCjc4PhfS=> NEjseG1,OTBizszN Mn7GS2k2OD1zMDDuUS=> NX34NlV6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1NFkyPjRpPkG1OVA6OTZ2PD;hQi=>
HCT-116 MWTjfZRwfG:6aXPpeJkh[XO|YYm= M2\TWJ4yOCEQvF2= MYjHTVUxRTNyIH7N MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTVyOUG2OEc,OTV3MEmxOlQ9N2F-
F-539 NF6wPJpkgXSxdH;4bYNqfHliYYPzZZk> MVL+NVAh|ryP NFjaeVZIUTVyPUGwJI5O MmnkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV3MEmxOlQoRjF3NUC5NVY1RC:jPh?=
UACC-62 M4rqUYN6fG:2b4jpZ4l1gSCjc4PhfS=> M2m2WZ4yOCEQvF2= M{LxXGdKPTB;MUCgcm0> M2O5clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NUC5NVY1Lz5zNUWwPVE3PDxxYU6=
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HCT116 M{nHNGN6fG:2b4jpZ4l1gSCjc4PhfS=> NGnsPYI4OiCqcoO= MnHvR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTGNVOTF4IHPlcIx{KGG|c3Xzd4VlKGG|IILl[JVkfGmxbjDpckBk\WyuII\pZYJqdGm2eTDpcoN2[mG2ZXSg[o9zKDd{IHjyd{BjgSCPVGSgZZN{[XluIFnDOVA:OS56ON88UU4> NYntZnRZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5NlM{QDhpPkK4PVI{Ozh6PD;hQi=>
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KB NGHpXVJEgXSxdH;4bYNqfHliYYPzZZk> NV\JWoxDPzJiaILz NX\kOGdsS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hU0JiY3XscJMh[W[2ZYKgO|IhcHK|IHL5JHNTSiCjc4PhfUwhUUN3ME2wMlA2|ryPLh?= NUPER4VJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMki5Nlc4QTBpPkK4PVI4PzlyPD;hQi=>
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MDA-MB-231 NEGz[FhEgXSxdH;4bYNqfHliYYPzZZk> MWe3NkBpenN? NILoflREgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOTEFvTVKtNlMyKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTDTVmIh[XO|YYmsJGlEPTB;MD6zNFfPxE1w M4nyNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ6OUK3O|kxLz5{OEmyO|c6ODxxYU6=
HL60 NELZeJJCdnSrcILvcIln\XKjdHn2[UBie3OjeR?= MlLuO|IhcHK| NVTWTWNpSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBpfW2jbjDIUFYxKGOnbHzzJIFnfGW{IEeyJIhzeyCkeTD0dplxcGGwIHLseYUhe3SjaX7pcoch[mG|ZXSgbIVu[XSxY4n0c41mfHKrYzDt[ZRpd2RuIFnDOVA:OC57Mt88UU4> NETSVlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUGzNVYyPid-MkmxN|E3OTZ:L3G+
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SW480 Mn:1SpVv[3Srb36gZZN{[Xl? M2jIbmlvcGmkaYTpc44hd2ZiRF7BJJRweG:rc3;t[ZJie2ViMTDpckBJd22xIIPhdIlmdnNiKHj1cYFvMSCVV{S4NEBk\WyuczygTWM2OD1|Lke1{txONg>? MmXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlUwLz6FaFXNRmw9N2F-
HCT116 M{\h[2Z2dmO2aX;uJIF{e2G7 MkjuTY5pcWKrdHnvckBw\iCGTlGgeI9xd2m|b33ldoF{\SBzIHnuJGhwdW9ic3HwbYVveyBqaIXtZY4qKEiFVEGxOkBk\WyuczygTWM2OD12Lke1{txONg>? M4rFNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY2Nyd-Q3jFUWJNRC:jPh?=
VACO 241 MoX2SpVv[3Srb36gZZN{[Xl? NEPJZ5VKdmirYnn0bY9vKG:oIFTORUB1d3CxaYPvcYVz[XOnIEGgbY4hUG:vbzDzZZBq\W6|IDjoeY1idiliVlHDU{AzPDFiY3XscJMtKEmFNUC9OE46Pc7:TT6= M3TMRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY2Nyd-Q3jFUWJNRC:jPh?=
SAS MoHRRY51cXC{b3zp[oVz[XSrdnWgZZN{[Xl? M3PjTlczKGi{cx?= MlrTRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDoeY1idiCVQWOgZ4VtdHNiaX7jeYJifGWmIH\vdkA4OiCqcoOgZpkhVVSWIHHzd4F6NCCLQ{WwQVbPxE1w M4[3d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFY2Nyd-Q3jFUWJNRC:jPh?=
MDA-MB-231 MorjR5l1d3SxeHnjbZR6KGG|c3H5 M2q0cFQ5KGi{cx?= M{j6b2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJG1FSS2PQj2yN|Eh[2WubIOgZYZ1\XJiNEigbJJ{KGK7IF3UWEBie3OjeTygTWM2OD1zNj60OO69VS5? NIPYOlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3PS9pPlPoSW1DVDxxYU6=
PC3 NWLhdmMyS3m2b4TvfIlkcXS7IHHzd4F6 NX:0PZVVPDhiaILz NHXWbWlEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBRSzNiY3XscJMh[W[2ZYKgOFghcHK|IHL5JG1VXCCjc4PhfUwhUUN3ME2xPE4zPM7:TT6= Mmf2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlUwLz6FaFXNRmw9N2F-
AGS M1r2PWFvfGmycn;sbYZmemG2aY\lJIF{e2G7 NELvdGc4OiCqcoO= MWLBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHj1cYFvKEGJUzDj[YxteyCrbnP1ZoF1\WRiZn;yJFczKGi{czDifUBOXFRiYYPzZZktKEmFNUC9NlMvPzcQvF2u Mnu1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlUwLz6FaFXNRmw9N2F-
MCF7 NYjFVnpITnWwY4Tpc44h[XO|YYm= NX2wOZJ2OTBidV2= NGK4SGEyOiCqcoO= MkXlVI9qe2:waX7nJIFkfGm4aYT5JIF1KHSxcH;pd49u\XKjc3WgNUBqdiCqdX3hckBOS0Z5IHPlcIx{KGG2IEGwJJVOKGGodHXyJFEzKGi{czDifUBqdW23bn;iZY5lKGSncHzleIlwdiCjc4PhfS=> MkGzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlUwLz6FaFXNRmw9N2F-
A549 M3HSc2Fxd3C2b4Ppd{Bie3OjeR?= M1q0e|EzKHSxIEG1JIhzew>? NXj4SIRbUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KG[xcn3heIlwdiCxZjDhdI9xfG:2aXOgZo9lcWW|IHHmeIVzKDF{IITvJFE2KGi{czDifUBndG:5IHP5eI9u\XS{aXOgcYV1cG:m NHzo[|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3PS9pPlPoSW1DVDxxYU6=
A549 NXGyZ4ZZSXCxcITvd4l{KGG|c3H5 MmXrNVIhfG9iMUWgbJJ{ MYrJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEF3NEmgZ4VtdHNiYYPz[ZN{\WRiYYOgZ4VtdCClb37k[Y5{[XSrb36gZYZ1\XJiMUKgeI8hOTViaILzJIJ6KG[ub4egZ5l1d22ndILpZ{Bu\XSqb3S= MlHTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOlUwLz6FaFXNRmw9N2F-
MDA-MB-231 NXXpWZB2S2WubDDjfYNt\SCjc4PhfS=> MnHhNUB2VQ>? M{TvSVE3KGi{cx?= MorWR4VtdCCleXPs[UBienKnc4SgbY4hcHWvYX6gUWRCNU2ELUKzNUBk\WyuczDhd5Nme3OnZDDhd{Bi[2O3bYXsZZRqd25iYYSgV{1xcGG|ZTDheEAyKHWPIHnuZ5Vj[XSnZDDmc5IhOTZiaILzJIJ6KG[ub4egZ5l1d22ndIL5 NHXSbGk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw3PS9pPlPoSW1DVDxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Cyclin1 / CDK1 / CDK2 ; 

PubMed: 29963130     


Western blot analysis indicated that camptothecin (6 µM) downregulates the expression of cell-cycle-associated proteins, cyclin 1, CDK1 and CDK2 in NPC cells. β-actin was used as a loading control.

Vimentin / Fibronectin / E-cadherin ; 

PubMed: 29963130     


Western blot analysis revealed that camptothecin (6 µM) treatment downregulates the expression of vimentin and fibronectin, and upregulates the expression of E-cadherin in NPC cells. β-actin was used as a loading control. NPC, nasopharyngeal carcinoma.

TGF-β / PI3K / pPI3K / AKT / pAKT ; 

PubMed: 29963130     


Western blot analysis revealed that camptothecin (6 µM) treatment inhibits the expression of TGF-β, PI3K and AKT in NPC cells. 

p53 / Cleaved caspase-3 / Cleaved PARP ; 

PubMed: 17548347     


NPCs in trophic factor-containing media were treated with 10 μM camptothecin for 0, 2, 4, 6, or 8 h, and extracts were immunoblotted for p53, active cleaved caspase-3, cleaved PARP, and total GSK3α/β as a loading control.

29963130 17548347
In vivo Camptothecin (8 mg/kg) displays complete growth inhibition and regression in mice xenografts of various tumors, including colon, lung, breast, stomach, and ovary tumors. [3] In mice, combinations of Camptothecin (50 mg/kg) and TNF (5 and 7 μg/kg), but not Camptothecin alone, induces liver damage. [4]

Protocol

Kinase Assay:[2]
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Topoisomerase I Cleavable Complex Assay:

Topoisomerase I is isolated from calf thymus and is devoid of topoisomerase II. All reactions are carried out in 10 mL volumes of reaction buffer (50 mM Tris-HCl, pH 7.5, 100 mM KCl, 0.5 mM EDTA, and 30 pg/mL BSA) in microtiter plates. Camptothecin is dissolved in DMSO at 10 mg/mL and serially diluted in 96-well microtiter plates to which the 32P end-labeled pBR322 DNA and topoisomerase enzyme are added. The reaction mixture is incubated at room temperature for 30 min and then the reaction stopped by adding 2 mL of a mixture of sodium dodecyl sulfate and proteinase K (1.6% and 0.14 mg/mL final concentrations, respectively). The plates are heated at 50 °C for 30 min, 10 mL of standard stop mixture containing 0.45 N NaOH is added in order to generate single-stranded DNA, and the samples are electrophoresed in 1.5% agarose gels in TBE buffer. Gels are blotted on nitrocellulose paper, dried, and exposed to X-ray film. The units of cleavage are calculated from the autoradiographs and plotted against the log drug concentration. The IC50 values are then obtaine
Cell Research:[2]
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  • Cell lines: U87MG, A549 and H838 cells
  • Concentrations: 0.17 nM–10 mM
  • Incubation Time: 48 hours
  • Method: Tumor cells are plated in 100 μL of medium in 96-well microtiter plates at a density of 1500 to 4000 cells per well and allowed to adhere overnight. Cells are incubated with Camptothecin for 48 hours and then with fresh medium for 48 hours. Camptothecin at each concentration is added in quadruplicate. Following a 4-hour incubation of treated cells with MTT, the reduced dye product is extracted from the cells with 0.2 mL of DMSO followed by 50 μL of Sorensen's buffer. The plates are shaken briefly, and the absorbance at 570 nm is read and quantitated. Curves are fitted to the MTT assay data using a four-parameter logistic equation.
    (Only for Reference)
Animal Research:[3]
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  • Animal Models: Nude mice (NIH-I high fertility strain) bearing xenografts of CASE, SW48, DOY, SPA, and CLO cells
  • Dosages: 0–8 mg/kg
  • Administration: Administered via i.m. or i.v. injection
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 3 mg/mL (8.61 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 348.35
Formula

C20H16N2O4

CAS No. 7689-03-4
Storage powder
in solvent
Synonyms NSC-100880, CPT, Campathecin, (S)-(+)-Camptothecin
Smiles CCC1(C2=C(COC1=O)C(=O)N3CC4=CC5=CC=CC=C5N=C4C3=C2)O

In vivo Formulation Calculator (Clear solution)

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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
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    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04419272 Not yet recruiting Drug: Methylphenidate|Other: Placebo Epilepsy VA New York Harbor Healthcare System|Portland VA Medical Center|Miami VA Healthcare System April 2022 Phase 4
NCT04657289 Not yet recruiting Drug: PDS Implant filled with 100 mg/mL Ranibizumab Neovascular Age-related Macular Degeneration (nAMD) Hoffmann-La Roche May 6 2021 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID