Obatoclax Mesylate (GX15-070)

Name: Obatoclax Mesylate (GX15-070)
Brand: Selleck Chemicals
SKU: S1057
Rating: 5 (106 reviews)

For research use only.

Catalog No.S1057

106 publications

Obatoclax Mesylate (GX15-070) Chemical Structure

CAS No. 803712-79-0

Obatoclax Mesylate (GX15-070) is an antagonist of Bcl-2 with K_i of 0.22 μM in a cell-free assay, can assist in overcoming MCL-1 mediated resistance to apoptosis. Phase 3.

Selleck's Obatoclax Mesylate (GX15-070) has been cited by 106 publications

Cancer Cell, 2019, 36(1):68-83

PubMed: 31257073 ( click the link to review the publication )

Nature, 2017, 551(7679):247-250

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Cell, 2010, 20;142(4):590-600.

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Cancer Res, 2021, canres.0955.2021

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Transl Oncol, 2021, 14(8):101116

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Biomaterials, 2020, 232:119709

PubMed: 31896513 ( click the link to review the publication )

Int J Mol Sci, 2020, 5;21(5) pii: E1773

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Invest New Drugs, 2020, 4

PubMed: 32367199 ( click the link to review the publication )

NPJ Breast Cancer, 2020, 6:30

PubMed: 32715085 ( click the link to review the publication )

Cell Chem Biol, 2019, 10.1016/j.chembiol.2019.04.011

PubMed: 31155509 ( click the link to review the publication )

Int J Mol Sci, 2019, 20(6)

PubMed: 30875757 ( click the link to review the publication )

Front Microbiol, 2019, 10:2236

PubMed: 31636613 ( click the link to review the publication )

Prostate, 2019, 79(11):1347-1359

PubMed: 31228231 ( click the link to review the publication )

Cancers (Basel), 2018, 10(12)

PubMed: 30563080 ( click the link to review the publication )

Cell Death Discov, 2018, 4:107

PubMed: 30479840 ( click the link to review the publication )

Cell Death Dis, 2018, 92(10)

PubMed: 23640461 ( click the link to review the publication )

Antioxidants (Basel), 2018, 10.3390/antiox7110150

PubMed: 30373097 ( click the link to review the publication )

BMC Cancer, 2018, 18(1):965

PubMed: 30305055 ( click the link to review the publication )

J Clin Invest, 2017, 127(1):153-168

PubMed: 27893461 ( click the link to review the publication )

Cancer Res, 2017, 77(13):3513-3526

PubMed: 28522750 ( click the link to review the publication )

Cancer Res, 2017, 10.1158/0008-5472.CAN-16-3424

PubMed: ( click the link to review the publication )

Genome Med, 2017, 9(1):40

PubMed: 28446242 ( click the link to review the publication )

J Exp Clin Cancer Res, 2017, 36(1):52

PubMed: 28403890 ( click the link to review the publication )

Arch Toxicol, 2017, 91(5):2191-2208

PubMed: 27807597 ( click the link to review the publication )
FEBS J, 2017, 284(2):222-236

PubMed: 27860276 ( click the link to review the publication )

Antimicrob Agents Chemother, 2017, 61(3)

PubMed: 27993855 ( click the link to review the publication )

Electrophoresis, 2017, 10.1002/elps.201600447

PubMed: 28608427 ( click the link to review the publication )

J Exp Med, 2017, 214(6):1691-1710

PubMed: 28450382 ( click the link to review the publication )

Oncotarget, 2017, 8(5):8670-8678

PubMed: 28055968 ( click the link to review the publication )

Viruses, 2017, 9(10)

PubMed: 28946654 ( click the link to review the publication )

Blood, 2016, 6;128(14):1834-1844

PubMed: 27465916 ( click the link to review the publication )

Nat Commun, 2016, 7:11190

PubMed: 27048913 ( click the link to review the publication )

Autophagy, 2016, 2;12(6):999-1014

PubMed: 27124102 ( click the link to review the publication )

Clin Cancer Res, 2016, 22(21):5370-5382

PubMed: 27178741 ( click the link to review the publication )

Proc Natl Acad Sci U S A, 2016, 113(46):13150-13155

PubMed: 27799526 ( click the link to review the publication )

ACS Appl Mater Interfaces, 2016, 8(21):13218-31

PubMed: 27160664 ( click the link to review the publication )

Antiviral Res, 2016, 126:69-80

PubMed: 26738783 ( click the link to review the publication )

Cancer Cell Int, 2016, 16:27

PubMed: 27042160 ( click the link to review the publication )

Int J Oncol, 2016, 49(3):953-60

PubMed: 27572939 ( click the link to review the publication )

Oncotarget, 2016, 7(3):3520-32

PubMed: 26657288 ( click the link to review the publication )

Tumour Biol, 2016, 37(8):10839-49

PubMed: 26880588 ( click the link to review the publication )

Oncotarget, 2016, 7(50):82013-82027

PubMed: 27852038 ( click the link to review the publication )

Mol Cancer Ther, 2016, 15(10):2357-2369

PubMed: 27512118 ( click the link to review the publication )

Oncotarget, 2016, 7(35):56253-56265

PubMed: 27494845 ( click the link to review the publication )

Oncotarget, 2016,

PubMed: 26910910 ( click the link to review the publication )

Oncotarget, 2016, 8(36):60060-60079

PubMed: 28947954 ( click the link to review the publication )

Oncotarget, 2016, 7(23):34453-71

PubMed: 27144341 ( click the link to review the publication )

Nat Chem Biol, 2015, 10.1038/nchembio.1986

PubMed: 26656090 ( click the link to review the publication )
Genes Dev, 2015, 29(20):2140-52

PubMed: 26494789 ( click the link to review the publication )

Int J Cancer, 2015, 137(12):2959-70

PubMed: 26096065 ( click the link to review the publication )

Cancer Lett, 2015, 10.1016/j.canlet.2015.05.008

PubMed: 25979228 ( click the link to review the publication )

Cell Death Dis, 2015, 6:e2014

PubMed: 26658189 ( click the link to review the publication )

Mol Oncol, 2015, 9(2):409-21

PubMed: 25308513 ( click the link to review the publication )

Mol Oncol, 2015, 9(2):409-21

PubMed: ( click the link to review the publication )

J Biol Chem, 2015, 290(34):20841-55

PubMed: 26124276 ( click the link to review the publication )

Cancer Cell Int, 2015, 15:97

PubMed: 26464561 ( click the link to review the publication )

Int J Oncol, 2015, 46(4):1844-8

PubMed: 25647738 ( click the link to review the publication )

Phys Chem Chem Phys, 2015, 17(39):25946-55

PubMed: 26120608 ( click the link to review the publication )

BMC Cancer, 2015, 15:919

PubMed: 26585594 ( click the link to review the publication )

BMC Cancer, 2015, 15:568

PubMed: 26231047 ( click the link to review the publication )

Open Biol, 2015, 5(3)

PubMed: 25761368 ( click the link to review the publication )

Mol Cells, 2015, 38(3):279-84

PubMed: 25666350 ( click the link to review the publication )

Oral Dis, 2015,

PubMed: 25482163 ( click the link to review the publication )

Cell Biochem Funct, 2015, 33(5):334-40

PubMed: 26178811 ( click the link to review the publication )

Anticancer Drugs, 2015, 26(3):331-42

PubMed: 25521557 ( click the link to review the publication )

Oncotarget, 2015,

PubMed: 26008975 ( click the link to review the publication )

Arch Pharm Chem Life Sci, 2015, 348: 1-11

PubMed: 25641608 ( click the link to review the publication )

J Pharm Pharmacol, 2015, 67(11):1575-84

PubMed: 26255619 ( click the link to review the publication )

Oncotarget, 2015, 6(40):42445-67

PubMed: 26636651 ( click the link to review the publication )

Oncotarget, 2015,

PubMed: 25749045 ( click the link to review the publication )

Cancer Lett, 2014, 348(1-2):20-8

PubMed: 24534203 ( click the link to review the publication )

Endocr Relat Cancer, 2014, 21(5):755-67

PubMed: 25012986 ( click the link to review the publication )
Oral Oncol, 2014, 50(2):120-7

PubMed: 24216166 ( click the link to review the publication )

Antimicrob Agents Chemother, 2014, 58(7):3689-96

PubMed: 24752266 ( click the link to review the publication )

Biochem Pharmacol, 2014, 92(1):55-61

PubMed: 25107702 ( click the link to review the publication )

J Biol Chem, 2014, 289(38):26481-91

PubMed: 25096574 ( click the link to review the publication )

Exp Cell Res, 2014, 322(1):217-25

PubMed: 24355809 ( click the link to review the publication )

Life Sci, 2014, 10.1016/j.lfs.2014.07.006

PubMed: 25066930 ( click the link to review the publication )

Oncol Rep, 2014, 32(6):2789-94

PubMed: 25333301 ( click the link to review the publication )

J Cell Biochem, 2014, 115(9):1624-35

PubMed: 24788582 ( click the link to review the publication )

PLoS One, 2014, 9(11):e111927

PubMed: 25409302 ( click the link to review the publication )

PLoS One, 2014, 9(10):e111146

PubMed: 25343454 ( click the link to review the publication )

PLoS One, 2014, 9(9):e106571

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Genes Cancer, 2014, 5(11-12):445-59

PubMed: 25568669 ( click the link to review the publication )

Erasmus University Rotterdam, 2014, 978-94-6259-077-9

PubMed: http://repub.eur.nl/pub/50827/ ( click the link to review the publication )

Assay Drug Dev Technol, 2014, 12(9-10):514-26

PubMed: 25506801 ( click the link to review the publication )

Proc Natl Acad Sci U S A, 2013, 110(11):4321-6

PubMed: 23447565 ( click the link to review the publication )

Int J Cancer, 2013, 133(8):1813-24

PubMed: 23580240 ( click the link to review the publication )

Cell Death Dis, 2013,

PubMed: 23681223 ( click the link to review the publication )

Cell Death Dis, 2013, 4:e742

PubMed: 23887633 ( click the link to review the publication )

Mol Cancer Ther, 2013, 12(4):448-59

PubMed: 23395885 ( click the link to review the publication )

J Immunol, 2013, 190(9):4621-6

PubMed: 23547112 ( click the link to review the publication )

Apoptosis, 2013, 18(10):1163-74

PubMed: 23887890 ( click the link to review the publication )

Am J Respir Cell Mol Biol, 2013, 49(4):609-18

PubMed: 23668615 ( click the link to review the publication )

Cancer Biol Ther, 2013, 14(10):982-96

PubMed: 24025251 ( click the link to review the publication )

Exp Cell Res, 2013, 319(10):1443-50

PubMed: 23416067 ( click the link to review the publication )
PLoS One, 2013, 8(2):e55982

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Helsinki Metropolia University, 2013, Jens Desloovere

PubMed: ( click the link to review the publication )

Cancer Res, 2012, 72(12):3069-79

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Oncogene, 2012, 32(37):4331-42

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Cancer Lett, 2012, 339(2):288-97

PubMed: 23200668 ( click the link to review the publication )

Cell Death Dis, 2012, 3:e351

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Cell Death Dis, 2012, 3:e299

PubMed: 22513873 ( click the link to review the publication )

Toxicol Sci, 2012, 128(1):79-91

PubMed: 22491427 ( click the link to review the publication )

Cancer Res, 2011, 71(13):4494-505

PubMed: 21555370 ( click the link to review the publication )

BMC Cancer, 2011, 11:24

PubMed: 21247487 ( click the link to review the publication )

Purity & Quality Control

Choose Selective Bcl-2 Inhibitors

Biological Activity

Description

Obatoclax Mesylate (GX15-070) is an antagonist of Bcl-2 with K_i of 0.22 μM in a cell-free assay, can assist in overcoming MCL-1 mediated resistance to apoptosis. Phase 3.

Features

Potential 1st-in-class small molecule antagonist of Bcl-2 designed to inhibit all relevant Bcl-2 family members, including Mcl-1.

Targets

Bcl-2 ^[1]
(Cell-free assay)

0.22 μM(Ki)

In vitro

Obatoclax completely inhibits Bak recovery of Mcl-1 at 5 μM in SK-Mel5 cells and overcomes resistance to ABT-373-induced apoptosis conferred by Mcl-1 in KB/Bcl-2 cells. ^[1] Obatoclax is a BH3 mimetic which binds to a broad spectrum of Bcl-2 family members, including Bcl-2, Bcl-xL, and Mcl-1. Obatoclax uniquely displaces BH3 domains by activation of the pocket of Mcl-1 followed by a triggering of apoptosis mediated by oligomerization of Bak and release of cytochrome c. Obatoclax is sensitive to Bcl-xL in cell lines lacking it or showing low expression. It shows low cytotoxicity to Mcl-1, Bcl-2, and Bcl-xL in all the strongly-expressed cell lines. Obatoclax inhibits multiple myeloma (MM) cell lines (KMS12PE, KMS18, MY5, etc.) with IC50 values ranging from 52 to 1100 nM and the inhibition is umimpaired even in the presence of IL-6 or IGF-1, which are resistance to cytotoxic agents, at a concentration of 150 nM. Obatoclax enhances the antimyeloma activity induced by melphalan, dexamethasone, or bortezomib. ^[2] Obatoclax potentiates TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-mediated apoptosis by unsequestering Bak and Bim from Bcl-2/Bcl-xL or Mcl-1 proteins in PANC-1 and BxPC-3 cells. ^[3]

Cell Data

Cell Lines	Assay Type	Activity Description	PMID
KARPAS-45	NVm2eW1YT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NYHnV2JtUUN3ME2wMlAxODB\|MUeg{txO	Mlf2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
SK-MEL-30	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mn3PTWM2OD1yLkCwNFM2PSEQvF2=	NFX2WGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
NALM-6	NHnSN4JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mn\3TWM2OD1yLkCwNFM2PiEQvF2=	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
KYSE-450	M4n3NGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M4HrdGlEPTB;MD6wNFA1PjlizszN	NEPVU3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
BHY	M4DpfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYrJR\|UxRTBwMECwOlAyKM7:TR?=	NVGzUoJRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
GR-ST	MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NVfoPVJYUUN3ME2wMlAxODZ2ODFOwG0>	NYPMcnhVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
786-0	NYLtb4o{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHTqTXdKSzVyPUCuNFAxPzd4IN88US=>	NHX0TlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
C-33-A	MlfpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NIjlbIdKSzVyPUCuNFAxQDR6IN88US=>	NU\COlljRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
BHT-101	MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEfxSIJKSzVyPUCuNFAyODZizszN	Mn\BQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
EB2	MnniS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NV7yT3F[UUN3ME2wMlAxOTF6IN88US=>	M2iwU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
G-361	NEj2WGpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mn7zTWM2OD1yLkCwNVI1KM7:TR?=	M2PpbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
KE-37	M1vhfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWDJR\|UxRTBwMECxNlgh\|ryP	Mm\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
MHH-PREB-1	MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MV3JR\|UxRTBwMECxOEDPxE1?	NUDmcINiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
MCF7	M1X6Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MoTqTWM2OD1yLkCwNVU6KM7:TR?=	NGLuVXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
LOUCY	NYDuVoJmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MUPJR\|UxRTBwMECxO\|Uh\|ryP	M1LnNFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
EW-7	M1frbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M2q1dWlEPTB;MD6wNFE4PSEQvF2=	MlHJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
KYSE-510	NWTKb4RjT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M4rYW2lEPTB;MD6wNFI{OyEQvF2=	NGj3SW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
SK-MEL-3	NEXOfYdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4LJXGlEPTB;MD6wNFI3PCEQvF2=	NV;JTWp{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
HAL-01	NH\3Z\|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NX;TOFB6UUN3ME2wMlAxOjl5IN88US=>	MnS5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
HCC2998	M1nvTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MWDJR\|UxRTBwMECzN\|Uh\|ryP	MkXUQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
A375	MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mly2TWM2OD1yLkCwN\|Uh\|ryP	NHL6R\|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
MZ7-mel	NH7UZ5lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1;MbWlEPTB;MD6wNFM6QSEQvF2=	NEHx[YI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
SH-4	NY[3N2lNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmDoTWM2OD1yLkCwOFE4KM7:TR?=	MnLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
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NCI-H1734	NX[xb4c4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NWm3UHJqUUN3ME2wMlQzODF\|IN88US=>	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
HDLM-2	NV7ndmtVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmrNTWM2OD1yLkSyNFM{KM7:TR?=	M4Gzc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
AU565	M4LqcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MVTJR\|UxRTBwNEK0Olgh\|ryP	NWLjenRrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
FADU	NGPnc\|hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MmfFTWM2OD1yLkSyOlQ6KM7:TR?=	MlLMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
OS-RC-2	M{HGN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Ml[0TWM2OD1yLkSzNFg{KM7:TR?=	MkXzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
CAMA-1	MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NX[3N29YUUN3ME2wMlQ{PjJ4IN88US=>	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
MONO-MAC-6	M2nlemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NYH5Tlc4UUN3ME2wMlQ{QDR7IN88US=>	M{XxT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
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SW982	Mkj1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHm5RZRKSzVyPUCuOFQ2OjhizszN	NGS0eo09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
SCC-3	MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NWfETIV3UUN3ME2wMlQ1PTd7IN88US=>	NXPvRmhQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
C32	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M3nqdWlEPTB;MD60OVIzQSEQvF2=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
BFTC-909	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NF7jT2lKSzVyPUCuOFUzQTlizszN	M{Xjb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
MEG-01	MmnnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MY\JR\|UxRTBwNEWzO\|Mh\|ryP	NF3oeGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
M14	M{HQdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NUnFWlRTUUN3ME2wMlQ2QTd7IN88US=>	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
MPP-89	NX7vT4dNT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHv1W\|FKSzVyPUCuOFYzPTZizszN	MnnEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
HCC1937	NIPufJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MUDJR\|UxRTBwNE[zPFQh\|ryP	MoHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
SW837	MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MoqwTWM2OD1yLkS2O\|IzKM7:TR?=	M3vVR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
DU-145	NXj5VVJJT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MojPTWM2OD1yLkS2O\|I6KM7:TR?=	M2jHV\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
LB996-RCC	MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MUnJR\|UxRTBwNEewOVQh\|ryP	NXXZfGs2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
C8166	MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M4PYc2lEPTB;MD60O\|Qh\|ryP	MmrWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
MOLT-4	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NGLMN5NKSzVyPUCuOFc4OzRizszN	NWToc2l2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
DMS-53	NE\hRYxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NHSwR4pKSzVyPUCuOFc5ODlizszN	MnvTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
TE-5	MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M3;wTWlEPTB;MD60PFMxPSEQvF2=	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
KYSE-520	NYn4b4pTT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MnTaTWM2OD1yLkS4N\|Q{KM7:TR?=	MkDZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
Detroit562	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MUDJR\|UxRTBwNEi4OkDPxE1?	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
SIMA	MnPlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M4P3Z2lEPTB;MD60PVQyPyEQvF2=	Moj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
NCI-H1651	M1vnbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NEPvRnhKSzVyPUCuOFk3PyEQvF2=	NGnyeZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
HCC1599	NYXlNIp3T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M3z0UGlEPTB;MD60PVk2PSEQvF2=	M2\ENFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
ABC-1	NFrITY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXHJR\|UxRTBwNUC0PVYh\|ryP	NFGwdGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
CAL-33	NYnOXVhVT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1zCOGlEPTB;MD61NFg1PCEQvF2=	NE\qVYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
OPM-2	MoHkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYLJR\|UxRTBwNUC5N\|ch\|ryP	NYjtfYlyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
RPMI-7951	MkmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYXJR\|UxRTBwNUC5OVch\|ryP	NHnmNo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
HT-1080	NUTj[ZByT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NY\ERoc6UUN3ME2wMlUyOTF7IN88US=>	NHXKV2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
LC4-1	NXrKW4pGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NEn0SGxKSzVyPUCuOVE1ODNizszN	MmLVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
OVCAR-3	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmPDTWM2OD1yLkWxOVch\|ryP	MoHLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
CAPAN-1	NIGxUJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MkXPTWM2OD1yLkWyNlg5KM7:TR?=	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
SK-OV-3	NELQWFdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MkGyTWM2OD1yLkWyOFM1KM7:TR?=	NGTpWoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
HuP-T3	NF3aepRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MmTaTWM2OD1yLkWyPFI4KM7:TR?=	NFTIT289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
NCI-H838	NX3hZ3dFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MYDJR\|UxRTBwNUK4OFkh\|ryP	NWSzUJRsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
K5	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MnrXTWM2OD1yLkWzNlAyKM7:TR?=	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
CAL-72	NGe3ZoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M1;VWGlEPTB;MD61N\|M5PyEQvF2=	M2\1e\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
NH-12	NIHMWotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MUDJR\|UxRTBwNUO5OVkh\|ryP	NUfQfnl5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
SNB75	MmLiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHfac\|ZKSzVyPUCuOVU4OyEQvF2=	NIPnTlk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
BPH-1	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MmLlTWM2OD1yLkW2N\|Q1KM7:TR?=	NXTSXGNJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
NOMO-1	NXXCeHM1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NH\HV2lKSzVyPUCuOVY{PzJizszN	MmjLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
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NCI-H2052	NFHE[pNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MYjJR\|UxRTBwNU[3OFEh\|ryP	Mo\FQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
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HSC-4	MnvUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MlXsTWM2OD1yLk[2Olk5KM7:TR?=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
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DK-MG	MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MV;JR\|UxRTBwN{GwNVYh\|ryP	NVjrbnUzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
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NCI-H510A	Ml;NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MlP0TWM2OD1yLke3NVg3KM7:TR?=	NUnqfotsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
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NCI-SNU-16	MmT3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M3Xw[mlEPTB;MUOuNVI2QCEQvF2=	NWDY[ZlRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
HD-MY-Z	NXLjbpF5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	Mmm1TWM2OD1zND62N\|Y{KM7:TR?=	MoG1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
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LB647-SCLC	NWS4PWFLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmnGTWM2OD1zNT63NVk2KM7:TR?=	NH;x[VQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
NCI-H345	MoPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Ml7ETWM2OD1zNz6zOFMh\|ryP	Mof3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
NCI-H1563	M1npe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NW[3bmpiUUN3ME2xPE44PjBzIN88US=>	NG\RUoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
RL	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MVzJR\|UxRTJzLkK2OVYh\|ryP	NH\3Soo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
KY821	MnjoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYDJR\|UxRTJ{LkC1OVMh\|ryP	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
JVM-3	M17XSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYLJR\|UxRTJ3LkC0NVMh\|ryP	NWPmVFJ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
NCI-H1793	MkH1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NUX0VFFMUUN3ME2yPE42PDl7IN88US=>	NUjOUnMxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
LU-134-A	NF:1dWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M{W4eGlEPTB;M{CuNlA{PyEQvF2=	NUXRR3p4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
NCI-H1395	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXzJR\|UxRTN{LkK0N\|Eh\|ryP	NG[wV2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
NCI-H1993	NWP2U2RCT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MV;JR\|UxRTN{LkiyNVgh\|ryP	NHTFbJY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
P31-FUJ	M4LUXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M3XENmlEPTB;M{OuOFg6QSEQvF2=	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
LS-513	NFP0WHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mn75TWM2OD1\|NT64PFc6KM7:TR?=	NIfkSIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmwzOTB5M{W4M{c,W0GQR1XSQE9iRg>?
SK-MEL-1	NYLCNpVUT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M{jjR2lEPTB;NECuN\|Q6QSEQvF2=	Mo\lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMNlExPzN3OD:nQnNCVkeHUkyvZV4>
SW684	MmmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MnHzTWM2OD12Mz65OlYyKM7:TR?=	NX3VNXpLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?
COR-L88	Mo\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MnfRTWM2OD12ND6wOlY2KM7:TR?=	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
NCI-H889	NF23U3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXzJR\|UxRTR3LkCwPFch\|ryP	M2e2UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFIyODd\|NUivK\|5USU6JRWK8M4E,
TALL-1	MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEWxVXdKSzVyPUS2MlEyQTlizszN	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQlyyNVA4OzV6Lze+V2FPT0WUPD;hQi=>
KARPAS-299	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NGrr[YpKSzVyPUS3MlE6QTNizszN	NXz0cVNwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNOjFyN{O1PE8oRlODTlfFVlww[T5?

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Cyclin D1 / p27 Kip1 ; PubMed: 28382170 Theranostics Western blotting showed that Obatoclax impacted cell cycle distribution through lowering cyclin D1 in K1 cells but in BCPAP cells, it impacted expression of p27 Kip1 rather than cyclin D1. p-Cyclin D1 ; PubMed: 28035994 Int J Mol Sci Cell line-dependent effect of obatoclax on cyclin D1 T286 phosphorylation. HCT116, HT-29, and LoVo cells were subject to 24 h-treatment with obatoclax (200 nM), followed by immunoblotting to evaluate the levels of threonine 286 (T286)-phosphorylated cyclin D1 (p-Cyclin D1) at indicated time points. α-Tubulin levels served as equal loading control; p-GSK3β / GSK3β / p-ERK / ERK / p-p38 / p38; PubMed: 28035994 Int J Mol Sci Obatoclax inhibits GSK3β but activates p38 (MAPK), while barely affecting ERK1/2 activity in HT-29 cells. HT-29 cells were exposed to 200 nM of obatoclax for 24 h. The status of activation of GSK3β, ERK1/2, and p38(MAPK) at indicated time points was determined by immunoblotting for corresponding phosphorylation. Specifically, serine 9-phosphorylation of GSK3β (p-GSK3β) reveals inhibition of GSK3β activity, whereas dual phosphorylation at threonine 202/tyrosine 204 of ERK1/2 (p-ERK1/2) and threonine 180/tyrosine 182 of p38 MAPK (p-p38) indicate activation of ERK1/2 and p38(MAPK), respectively. α-Tubulin levels served as equal loading control;	28382170 28035994
Growth inhibition assay	Cell proliferation ; PubMed: 28035994 Int J Mol Sci Obatoclax inhibits colorectal cancer cell proliferation. Human colorectal carcinoma cell lines HCT116, HT-29, and LoVo cells were treated with obatoclax (0, 50, 100, 200 nM) for 24, 48, and 72 h. The numbers of cells at each time point were counted using a Neubauer chamber. Data were presented as the percentage relative to the drug-untreated control. Cell viability; PubMed: 21368880 Cell Death Dis H727 NSCLC cells are resistant to obatoclax. H727 and H1975 NSCLC cells were treated with obatoclax at the indicated doses for 48 (left) or 72 h (right), and the viability of cells determined by ViaLight assay. The EC50 values of cells at 48 h were; H1975 267 nM; H727 3.2 μM. The EC50 values of cells at 72 h were; H1975 66 nM; H727 621 nM. Data are expressed as mean±S.D.	28035994 21368880

In vivo

Obatoclax also exhibits high antitumor activity in SCID mice bearing human C33A cerivical carcinoma tumors at a dose of 0.5 mg/kg. ^[1]

Protocol

Kinase Assay:^[1]	- Collapse Bcl-2 Binding affinity calculation: A predicted binding affinity for Obatoclax binding to BCL-2 is calculated using the SIE scoring function. ^[4] As a control in determining the reliability of the calculation, predicted binding affinities K_i) are calculated for a set of 12 small molecules with experimentally measured binding affinities to BCL-2.
Cell Research:^[2]	- Collapse Cell lines: Human MM cells (HMCLs), peripheral blood lymphocytes (PBLs), bone marrow stromal cells (BMSCs) Concentrations: ~10 μM Incubation Time: 48-72 hours Method: Obatoclax is dissolved in DMSO at a stock concentration of 5 mM. Cell viability is assayed by MTT. Human MM cells (HMCLs), peripheral blood lymphocytes (PBLs), bone marrow stromal cells (BMSCs) are seeded in 96-well plates at a density of 2 × 10⁴ per well for HMCLs or 5~10 × 10³ for PBLs. Various concentrations of Obatoclax are added to the cells, with or without IGF-1 (50 ng/mL) or IL-6 (10 ng/mL). Cells are incubated for 48-72 hours and cell viability is determined. (Only for Reference)
Animal Research:^[1]	- Collapse Animal Models: Female BALB/c or CB17 SCID/SCID mice bearing SW480, C33A, PC3, and 4T1 cells are used. Dosages: 0.0313, 0.25, 0.5 and 2 mg/kg Administration: Administered intravenously (tail vein) once a day (Only for Reference)

References

[4] Naim M, et al. J Chem Inf Model, 2007, 47(1), 122-133.

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Solubility (25°C)

In vitro	DMSO	83 mg/mL (200.73 mM)
	Water	Insoluble
	Ethanol	Insoluble
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 30% PEG400+0.5% Tween80+5% propylene glycol For best results, use promptly after mixing.	30 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Obatoclax Mesylate (GX15-070) SDF

Molecular Weight	413.49
Formula	C₂₀H₁₉N₃O.CH₄O₃S
CAS No.	803712-79-0
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	N/A
Smiles	CC1=CC(=C(N1)C=C2C(=CC(=C3C=C4C=CC=CC4=N3)N2)OC)C.CS(=O)(=O)O

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

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Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-09-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT00438178	Completed	Drug: Obatoclax mesylate (GX15-070MS)	Hematological Malignancies	Gemin X\|Teva Pharmaceutical Industries	October 2005	Phase 1
NCT00600964	Completed	Drug: GX15-070MS	Chronic Lymphocytic Leukemia	Gemin X\|Teva Pharmaceutical Industries	September 2004	Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Bcl-2 Signaling Pathway Map

Bcl-2 Inhibitors with Unique Features

Pan Bcl-2 Inhibitor

ABT-737 : Bcl-xL, EC50=78.7 nM; Bcl-2, EC50=30.3 nM; Bcl-w, EC50=197.8 nM.
Most Potent Bcl-2 Inhibitor

ABT-263 (Navitoclax) : Bcl-xL, K_i≤ 0.5 nM; Bcl-2, K_i≤ 1 nM; Bcl-w, K_i≤ 1 nM.
Classic Bcl-2 Inhibitor

ABT-737 : BH3-mimetic inhibitor, Bcl-xL, EC50=78.7 nM; Bcl-2, EC50=30.3 nM; Bcl-w, EC50=197.8 nM.

Related Bcl-2 Products

MI-773 (SAR405838) ^New

MI-773 (SAR405838) is an orally available MDM2 antagonist with K_i of 0.88 nM. Phase 1.
Navitoclax (ABT-263)

Navitoclax (ABT-263) is a potent inhibitor of Bcl-xL, Bcl-2 and Bcl-w with K_i of ≤ 0.5 nM, ≤1 nM and ≤1 nM in cell-free assays, but binds more weakly to Mcl-1 and A1. Phase 2.
Venetoclax (ABT-199)

Venetoclax (ABT-199, GDC-0199) is a Bcl-2-selective inhibitor with K_i of <0.01 nM in cell-free assays, >4800-fold more selective versus Bcl-xL and Bcl-w, and no activity to Mcl-1. Venetoclax is reported to induce cell growth suppression, apoptosis, cell cycle arrest, and autophagy in triple negative breast cancer MDA-MB-231 cells. Phase 3.

Features:Re-engineered version of ABT-263 (Navitoclax).
ABT-737

ABT-737 is a BH3 mimetic inhibitor of Bcl-xL, Bcl-2 and Bcl-w with EC50 of 78.7 nM, 30.3 nM and 197.8 nM in cell-free assays, respectively; no inhibition observed against Mcl-1, Bcl-B or Bfl-1. ABT-737 induces mitochondrial pathway apoptosis and mitophagy. Phase 2.
Sabutoclax

Sabutoclax (BI-97C1) is a pan-Bcl-2 inhibitor, including Bcl-xL, Bcl-2, Mcl-1 and Bfl-1 with IC50 of 0.31 μM, 0.32 μM, 0.20 μM and 0.62 μM, respectively.
(R)-(-)-Gossypol acetic acid

(R)-(-)-Gossypol (AT-101) acetic acid, the R-(-) enantiomer of Gossypol acetic acid, binds with Bcl-2, Bcl-xL and Mcl-1 with K_i of 0.32 μM, 0.48 μM and 0.18 μM in cell-free assays; does not inhibit BIR3 domain and BID. AT-101 simultaneously triggers apoptosis and a cytoprotective type of autophagy. Phase 2.

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Obatoclax Mesylate (GX15-070)