Name: BAI1
Brand: Selleck Chemicals
SKU: S8865
Rating: 5 (4 reviews)

BAI1 is a direct allosteric inhibitor of BAX with a dissociation constant (Kd) of 15.0 ± 4 μM.

BAI1 Chemical Structure

CAS: 335165-68-9

Selleck's BAI1 has been cited by 4 publications

Purity & Quality Control

Batch: S886501 DMSO] 18 mg/mL] false] Ethanol] 3 mg/mL] false] Water] Insoluble] false Purity: 99.94%

99.94

BAI1 Related Products

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Signaling Pathway

Bcl-2 Signaling Pathway

Choose Selective Bcl-2 Inhibitors

Biological Activity

Description

BAI1 is a direct allosteric inhibitor of BAX with a dissociation constant (Kd) of 15.0 ± 4 μM.

Targets

Bax ^[1]
(Cell-free assay)

15.0 μM(Kd)

In vitro
In vitro	BAX activation inhibitor 1 (BAI1) demonstrates inhibition of tBID-induced BAX-mediated membrane permeabilization in a dose dependent manner with an IC50 of 3.3 μM. BAI1 binds to monomeric BAX at a novel allosteric site. BAI1 is a potent inhibitor of BAX conformational activation and oligomerization in solution, not by direct competition with the activator, but rather through an allosteric mechanism that favors the inactive BAX. Moreover, BAI1 inhibited BAX membrane association and translocation induced either by tBID (IC50 = 5 ± 1 μΜ) or BIM SAHB (IC50 = 2 ± 1 μΜ) in a dose dependent manner and with a similar IC50 to BAX-mediated liposomal release. BAI1 selectively inhibits BAX-dependent cell death^[1].
Cell Research	Cell lines	MEFs
	Concentrations	0-10 μM
	Incubation Time	8 h
	Method	Caspase 3/7 assay of WT MEFs upon pro-apoptotic TNFα/CHX treatment in the absence or presence of co-treatment with BAI1 at indicated doses for 8 hours.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT05477862	Not yet recruiting	Alzheimer Disease	Northwestern University	August 15 2023	Not Applicable

References

[1] Garner TP, et al. Nat Chem Biol. 2019, 15(4):322-330.

Chemical Information & Solubility

Molecular Weight	467.20	Formula	C₁₉H₂₁Br₂N₃O
CAS No.	335165-68-9	SDF	--
Smiles	C1CN(CCN1)CC(CN2C3=C(C=C(C=C3)Br)C4=C2C=CC(=C4)Br)O.Cl.Cl
Storage (From the date of receipt)	3 years -20°C powder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years -20°C powder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 18 mg/mL ( (38.52 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 3 mg/mL

Water : Insoluble

Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.

In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
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