For research use only.

Catalog No.S7790

40 publications

A-1210477 Chemical Structure

CAS No. 1668553-26-1

A-1210477 is a potent and selective MCL-1 inhibitor with Ki and IC50 of 0.454 nM and 26.2 nM, respectively, >100-fold selectivity over other Bcl-2 family members.

Selleck's A-1210477 has been cited by 40 publications

Purity & Quality Control

Choose Selective Bcl-2 Inhibitors

Biological Activity

Description A-1210477 is a potent and selective MCL-1 inhibitor with Ki and IC50 of 0.454 nM and 26.2 nM, respectively, >100-fold selectivity over other Bcl-2 family members.
MCL-1 [1]
(Cell-free assay)
26.2 nM
In vitro

In H929 cells, A-1210477 binds to MCL-1 with high affinity and induces MCL-1 protein elevation. In H929, H2110, and H23 cells, A-1210477 induce the hallmarks of apoptosis, and inhibits MCL-1-dependent cell viability. A-1210477 also synergizes with navitoclax to kill a variety of cancer cell lines. [1] In SKBR3 cells, A-1210477 inhibits MCL-1–BIM interaction and induces classical features of apoptosis. [2] In addition, A-1210477 sensitizes non-Hodgkin's lymphoma cell lines to venetoclax (ABT-199). [3]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human H23 cells NXfrdWFIS3m2b4TvfIlkcXS7IHHzd4F6 NUHrW5RvOjRiaILz MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDINlMh[2WubIOgbY5kfWKjdHXkJIZweiB{NDDodpMh[nliQ1PLPEBie3OjeR?= MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8{OTN6OU[5PUc,OzF|OEm2PVk9N2F-

... Click to View More Cell Line Experimental Data

Methods Test Index PMID
Western blot

PubMed: 30796196     

NB4 cells were incubated with 3 µM A-1210477 for 0-24 h or 10-20 µM Gossypol for 6 h. 

PARP / MEK / Cyt c/ Tom20 ; 

PubMed: 30796196     

Jurkat wt, c3, and c4 cells were incubated with 0–20 µM A-1210477 for 6 h. Digitonin permeabilization was performed to assess cytochrome c release. All incubations included 10 µM QVD to prevent loss of cytochrome c from apoptotic cells. PARP, MEK1/2, and Tom20 were used as fraction controls.

pERK / ERK / p-MCL1 / Caspase-3 ; 

PubMed: 27765849     

CRC (RKO, HT29) and melanoma (A375) cell lines were treated with A-1210477 alone or combined with cobimetinib for 48 h at the indicated doses. ERK activation, BIM isoform expression, and cleavage of PARP and CASPASE3 were analyzed by immunoblotting.

30796196 27765849


Kinase Assay:[1]
- Collapse

Binding affinity assays:

TR-FRET-binding affinity assays are performed for BCL-2, BCL-XL, and MCL-1 in 4.52 mM monobasic potassium phosphate, 15.48 mM dibasic potassium phosphate, 1 mM sodium EDTA, 0.05% Pluronic F-68 detergent, 50 mM sodium chloride, and 1 mM DTT (pH 7.5). For MCL-1 assays, GST-tagged MCL-1 (1 nM) is mixed with 100 nM f-Bak, 1 nM Tb-labeled anti-GST antibody, and compound at room temperature (RT) for 60 min. Fluorescence is measured on an Envision plate reader using a 340/35 nm excitation filter and 520/525 (f-Bak) and 495/510 nm (Tb-labeled anti-GST antibody) emission filters.
Cell Research:[1]
- Collapse
  • Cell lines: H929, H2110, and H23 cells
  • Concentrations: ~30 μM
  • Incubation Time: 48 h or 72 h
  • Method: Adherent cell lines are seeded at 50 000 cells per well in 96-well plates and treated for 48 h with compounds diluted in half-log steps starting at 30 μM and ending at 0.001 μM. Multiple myeloma cell lines were seeded at 15 000–20 000 cells per well and treated similarly. Effects on proliferation and viability were determined using CellTiter-Glo reagent according to the manufacturer’s instructions. IC50 values are determined by non-linear regression analysis of the concentration response data.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 3 mg/mL warmed (3.52 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 850.04


CAS No. 1668553-26-1
Storage powder
in solvent
Synonyms N/A

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Bcl-2 Signaling Pathway Map

Related Bcl-2 Products

Tags: buy A-1210477|A-1210477 ic50|A-1210477 price|A-1210477 cost|A-1210477 solubility dmso|A-1210477 purchase|A-1210477 manufacturer|A-1210477 research buy|A-1210477 order|A-1210477 mouse|A-1210477 chemical structure|A-1210477 mw|A-1210477 molecular weight|A-1210477 datasheet|A-1210477 supplier|A-1210477 in vitro|A-1210477 cell line|A-1210477 concentration|A-1210477 nmr|A-1210477 in vivo|A-1210477 clinical trial|A-1210477 inhibitor|A-1210477 Apoptosis inhibitor
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID