For research use only.

Catalog No.S8061 Synonyms: BI-97C1

7 publications

Sabutoclax Chemical Structure

CAS No. 1228108-65-3

Sabutoclax (BI-97C1) is a pan-Bcl-2 inhibitor, including Bcl-xL, Bcl-2, Mcl-1 and Bfl-1 with IC50 of 0.31 μM, 0.32 μM, 0.20 μM and 0.62 μM, respectively.

Selleck's Sabutoclax has been cited by 7 publications

2 Customer Reviews

  • (e) Abs (490 nm) was measured by MTS assay in siControl or siPTBP1 treated PC3 cells with DMSO, 1000 nM ABT737, or 100 nM Sabutoclax for 48 h, showing no significant change in cell viability following siPTBP1 treatment in DMSO control, ABT737, or Sabutoclax treated cells. (f) PC3 cells transfected with siControl or a mixture of two siPTBP1 were treated with 1000 nM ABT737 or 100 nM Sabutoclax combined with various doses of docetaxel for 48 h and cell viability was assessed by MTS assay. The viability of cells with 1000 nM ABT737 or 100 nM Sabutoclax alone was set as 100%. All data are presented as mean±S.E.M., n=3. The statistical significance was determined by unpaired student t-test where *P<0.05; **P<0.01; ***P<0.001.

    Cell Death Differ, 2016, 23(10):1681-90.. Sabutoclax purchased from Selleck.

    Expression levels of cleaved PARP and cleaved caspase-3 in CALDOX cells, as well as expression levels of Bim, Puma, Bax and Survivin in MCF-7/A02 and CALDOX cells determined by western blotting after sabutoclax treatment for 48 h. Numerical data are presented as mean±S.D. of three independent experiments. *P<0.05

    Cancer Lett, 2018, 423:47-59. Sabutoclax purchased from Selleck.

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Choose Selective Bcl-2 Inhibitors

Biological Activity

Description Sabutoclax (BI-97C1) is a pan-Bcl-2 inhibitor, including Bcl-xL, Bcl-2, Mcl-1 and Bfl-1 with IC50 of 0.31 μM, 0.32 μM, 0.20 μM and 0.62 μM, respectively.
Mcl-1 [1]
(Cell-free assay)
Bcl-xL [1]
(Cell-free assay)
Bcl-2 [1]
(Cell-free assay)
Bfl-1 [1]
(Cell-free assay)
0.20 μM 0.31 μM 0.32 μM 0.62 μM
In vitro

BI-97C1 potently inhibits cell growth of human prostate cancer, lung cancer, and lymphoma cell lines with EC50 values of 0.13, 0.56, and 0.049 μM, respectively, and shows little cytotoxicity against bax-/-bak-/- cells[1]. It is suggest that treatment with the combination regimen of mda-7/IL-24 and BI-97C1 induces autophagy that facilitates apoptosis in association with up-regulation of NOXA, accumulation of Bim, and activation of Bax and Bak[2].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
human H460 cells  NVHqcmo2S3m2b4TvfIlkyqCjc4PhfS=> MVOzJIRigXN? MWnDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDIOFYxKGOnbHzzJIFnfGW{IEOg[IF6eyCkeTDBWHAuVEmWRTDhd5NigSxiRVO1NF0xNjd6IN88US=> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR2M{[yO{c,OjB2NEO2Nlc9N2F-
human PC3 cells MoHMR5l1d3SxeHnjxsBie3OjeR?= M4H5cFMh\GG7cx?= MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDQR|Mh[2WubIOgZYZ1\XJiMzDkZZl{KGK7IFHUVE1NUVSHIHHzd4F6NCCHQ{WwQVQvPjRizszN MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR2M{[yO{c,OjB2NEO2Nlc9N2F-
MEF  MnH3SpVv[3Srb36gZZN{[Xl? NIXzTXg{OCEQvF2= M4rWUVI1KGh? M1LlVmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gUWVHKGW6cILld5Nqdmdid3ns[EB1gXCnIFLjcE0zKHC{b4TlbY4h[XRiM{CgeW0h[W[2ZYKgNlQhcHK|IHL5JGZKXENvY3;ubpVo[XSnZDDhco5mgGmwIG[gZY5lKHC{b4Dp[Il2dSCrb3Tp[IUhe3SjaX7pcocu[mG|ZXSgSmFEWyCjbnHsfZNqew>? NYrXTVZDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OFM3OjdpPkKwOFQ{PjJ5PD;hQi=>
BP3 NF\abFBCeG:ydH;zbZMh[XO|YYm= M3vCZ|EhfG9iMjDkZZl{ MVzJcoR2[3Srb36gc4Yh[XCxcITvd4l{KGmwIHj1cYFvKEKSMzDj[YxteyCjZoTldkAyKHSxIEKg[IF6eyCkeTDGTXRENWOxbnr1[4F1\WRiYX7u[ZhqdiCYIHHu[EBxem:yaXTpeY0hcW:maXTlJJN1[WmwaX7nMYJie2WmIF\BR3Mh[W6jbInzbZMtKEWFNUC9NE4xPDoQvF2= MkDIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NEO2NlcoRjJyNESzOlI4RC:jPh?=
M2182 M3;hcGFvfGm2dX3vdkBie3OjeR?= MnfuNUB1dyB3IH3nM4to NHjOO2RCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBO[2xvMTDveoVz\XiycnXzd4lv\yCqdX3hckBOOjF6MjDj[YxteyC6ZX7v[5Ji\nSnZDDpckBDSUyEL3OgcY92e2ViYYPz[ZN{\WRiYYOgdoVlfWO2aX;uJI9nKHS3bX;yJJNqgmViYYSgNUB1dyB3IH3nM4toNCCrcDDh[I1qdmm|dHXy[YQhQSC2aX3ld{BmfmW{eTCyJIRigXN? M2jJUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNESzOlI4Lz5{MES0N|YzPzxxYU6=
M2182 M4rOV2FvfGm2dX3vdkBie3OjeR?= MUm1JI1oN2up MUDBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDNZ4wuOSCxdnXy[ZhxemW|c3nu[{BpfW2jbjDNNlE5OiClZXzsd{B5\W6xZ4Lh[pRm\CCrbjDCRWxDN2NibX;1d4Uh[XO|ZYPz[YQh[XNiY3;tdIxmfGViaX7obYJqfGmxbjDv[kB1fW2xcjDndo94fGhiYYSgOUBu\y:tZzygbZAh[WSvaX7pd5RmemWmIEmgeIlu\XNiZY\ldpkhOiCmYYnz M3LnPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNESzOlI4Lz5{MES0N|YzPzxxYU6=

... Click to View More Cell Line Experimental Data

In vivo BI-97C1 displays in vivo efficacy in transgenic mice in which Bcl-2 is overexpressed in splenic B-cells and also demonstrates superior single-agent antitumor efficacy in a prostate cancer mouse xenograft model that depends on Mcl-1 for survival[1]. Treatment with Ad.5/3-mda-7 and BI-97C1 significantly inhibits the growth of human PC xenografts in nude mice and spontaneously induced PC in Hi-myc transgenic mice. Tumor growth inhibition correlats with increased TUNEL staining and decreased Ki-67 expression in both PC xenografts and prostates of Hi-myc mice[2].


Kinase Assay:[1]
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Competitive fluorescence polarization assays (FPA) :

A Bak BH3 peptide (F-BakBH3) (GQVGRQLAIIGDDINR) is labeled at the N-terminus with fluorescein isothiocyanate (FITC) and purified by HPLC. For competitive binding assays, 100 nM GST-Bcl-XL ΔTM protein is preincubated with the tested compound at varying concentrations in 47.5 μL PBS (pH = 7.4) in 96-well black plates at room temperature for 10 min, and then 2.5 μL of 100 nM FITC-labeled Bak BH3 peptide is added to produce a final volume of 50 μL. The wild-type and mutant Bak BH3 peptides are included in each assay plate as positive and negative controls, respectively. After 30 min incubation at room temperature, the polarization values in millipolarization units are measured at excitation/emission wavelengths of 480/535 nm with a multilabel plate reader. IC50 is determined by fitting the experimental data to a sigmoidal dose-response nonlinear regression model. Data reported are mean of three independent experiments. Performance of Bcl-2 and Mcl-1FPA are similar. Briefly, 50 nM of GST-Bcl-2 or -Mcl-1are incubatedwith various concentrations of compound (4 and 11-14) for 2 min, and then 15 nM FITC-conjugated-Bim BH3 peptide is added in PBS buffer. Fluorescence polarization is measured after 10 min.
Cell Research:[1]
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  • Cell lines: PC3, H460, H1299
  • Concentrations: ~1 μM
  • Incubation Time: 72 h
  • Method: ATP-LITE assay
    (Only for Reference)
Animal Research:[1]
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  • Animal Models: Bcl-2 transgenic mice, human prostate cancer xenografts
  • Dosages: 1 mg/kg, 3 mg/kg, 5 mg/kg
  • Administration: intraperitoneally
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (142.69 mM)
Ethanol 54 mg/mL warmed (77.05 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 700.78


CAS No. 1228108-65-3
Storage powder
in solvent
Synonyms BI-97C1
Smiles CC1=CC2=C(C(=C(C=C2C(=C1C3=C(C4=CC(=C(C(=C4C=C3C)C(=O)NCC(C)C5=CC=CC=C5)O)O)O)O)O)O)C(=O)NCC(C)C6=CC=CC=C6

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID