Home Metabolism phosphatase inhibitor Sodium Monofluorophosphate

Sodium Monofluorophosphate

Catalog No.S4013 Synonyms: NSC248

For research use only.

Sodium Monofluorophosphate (NSC248) is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.

Sodium Monofluorophosphate Chemical Structure

CAS No. 10163-15-2

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Biological Activity

Description Sodium Monofluorophosphate (NSC248) is a competitive inhibitor of pyruvate kinase and alkaline phosphatase with Ki of 3.4 mM and 69 μM, respectively, which also irreversibly inhibits phosphorylase phosphatase with Ki of 0.5 mM.
Targets
alkaline phosphatase [1]
()		 phosphorylase phosphatase [1]
()		 pyruvate kinase [1]
()
69 μM(Ki) 0.5 mM(Ki) 3.4 mM(Ki)
In vitro

Monofluorophosphate (1 mM) induces a three-fold increase in proliferation rate of embryonic chick calvarial cells in serum-free monolayer cultures. 30 μM of Monofluorophosphate increases the activity of DNA and alkaline phosphatase in calvarial cells by 135% and 178%, respectively. In cultured intact calvaria, 30 μM of Monofluorophosphate increases DNA activity, alkaline phosphatase activity and collagen production by 143%, 186% and 151%, respectively. [2]
In vivo Monofluorophosphate displays potent activity on bone immobilization in vivo. Rats supplied with drinking water containing 50 ppm F as Monofluorophosphate show a significant increase in the bone sickness of mobile tibia and immobile tibia, compared with fluoride-free water group (1.48 vs 0.98 mm, 0.79 vs 0.40 mm, respectively). Monofluorophosphate does not change the specific gravities of the immobilized and non-immobilized tibiae of the rats. Administration of Monofluorophosphate increases the mineralization rate of bones and counteracts the decrease in bone thickness, ash content and specific gravity induced by immobilization, without changing the Ca/P ratio of the ash. [3]

Protocol (from reference)

Solubility (25°C)

In vitro

 Water 29 mg/mL
(201.45 mM)
DMSO Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 143.95
Formula

FNa2O3P
CAS No. 10163-15-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles [O-]P(=O)([O-])F.[Na+].[Na+]

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