β-Glycerophosphate sodium salt hydrate

Catalog No.S3620

For research use only.

β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor.

β-Glycerophosphate sodium salt hydrate Chemical Structure

CAS No. 13408-09-8

Selleck's β-Glycerophosphate sodium salt hydrate has been cited by 4 Publications

Purity & Quality Control

Choose Selective phosphatase Inhibitors

Biological Activity

Description β-Glycerophosphate (sodium salt hydrate) is a potent protein phosphatase inhibitor.
Targets
phosphatase [1]
()
In vitro

β-glycerophosphate accelerates in vitro calcification by BVSMC and induces extensive calcium deposition in a manner analogous to in vitro mineralization by osteoblasts. β-glycerophosphate can increase ALP activity in osteoblasts[2].

Protocol (from reference)

Cell Research:[2]
  • Cell lines: BVSMC (bovine vascular smooth muscle cells)
  • Concentrations: 10 mmol/L
  • Incubation Time: 14 d
  • Method: BVSMC are cultured in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS (growing medium). After confluence, the cells are inoculated in DMEM containing 10 mmol/L sodium pyruvate supplemented with 15% FBS in the presence of 10 mmol/L β-glycerophosphate, 10-7 mol/L insulin, and 50 μg/mL of ascorbic acid for 14 days (calcification medium). The medium is replaced with fresh medium every 3 days. In certain experiments, only β-glycerophosphate is used to induce BVSMC calcification. Moreover, in the time-course experiments, before being cultured in calcification medium (defined as day 0), cells are selected to be used as controls.

Solubility (25°C)

In vitro

Water 61 mg/mL
(199.27 mM)
DMSO Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 306.11
Formula

C3H9O6P.5H2O.2Na

CAS No. 13408-09-8
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C(C(CO)OP(=O)([O-])[O-])O.O.O.O.O.O.[Na+].[Na+]

In vivo Formulation Calculator (Clear solution)

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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