Home Metabolism phosphatase inhibitor Sephin-1 For research use only.

Sephin-1

Synonyms: NSC-65390

Sephin-1 (NSC-65390) is a selective inhibitor of a holophosphatase, a small molecule that safely and selectively inhibits a regulatory subunit of protein phosphatase 1 in vivo. Sephin-1 (NSC-65390) selectively bounds and inhibits the stress-induced PPP1R15A.

Sephin-1 Chemical Structure

Sephin-1 Chemical Structure

CAS: 951441-04-6

Purity & Quality Control

Batch: S639101 DMSO] 39 mg/mL] false] Ethanol] 39 mg/mL] false] Water] Insoluble] false Purity: 99.83%
99.83

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Choose Selective phosphatase Inhibitors

Biological Activity

Description Sephin-1 (NSC-65390) is a selective inhibitor of a holophosphatase, a small molecule that safely and selectively inhibits a regulatory subunit of protein phosphatase 1 in vivo. Sephin-1 (NSC-65390) selectively bounds and inhibits the stress-induced PPP1R15A.
Targets
holophosphatase [1]
In vitro
In vitro

Sephin1 stimulates ATF4 synthesis in the absence of stress.[2]
Cell Research Cell lines HEK293 and HeLa cells
Concentrations 10 µM
Incubation Time 1.5, 3, 6, 18 to 24 h
Method

To characterize UPR activation, HEK293 cells and primary cortical neurons were treated with Thap (100 nM) alone or in combination with the UPR modulators described above [ISRIB (500 nM), GSK (2 µM), or 4µ8C (5, 16, and 50 µM), and Sephin1 (10 µM), and DMSO as a control]. Cells were harvested for Western blot (WB) analysis at different time points after treatment (from 1.5, 3, 6, 18 to 24 h).

Chemical Information & Solubility

Molecular Weight 196.64 Formula

C8H9ClN4
CAS No. 951441-04-6 SDF --
Smiles NC(=N)N\N=C\C1=CC=CC=C1Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 39 mg/mL ( (198.33 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 39 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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