Sanguinarine chloride

Catalog No.S5452

For research use only.

Sanguinarine, a plant alkaloid, is a potent and specific protein phosphatase (PP) 2C inhibitor. Sanguinarine chloride can stimulate apoptosis via activating the production of reactive oxygen species (ROS). Sanguinarine-induced apoptosis is associated with the activation of JNK and NF-κB.

Sanguinarine chloride Chemical Structure

CAS No. 5578-73-4

Selleck's Sanguinarine chloride has been cited by 1 Publication

Purity & Quality Control

Choose Selective phosphatase Inhibitors

Biological Activity

Description Sanguinarine, a plant alkaloid, is a potent and specific protein phosphatase (PP) 2C inhibitor. Sanguinarine chloride can stimulate apoptosis via activating the production of reactive oxygen species (ROS). Sanguinarine-induced apoptosis is associated with the activation of JNK and NF-κB.
Targets
PP2C [1]
(Cell-free)
0.68 μM(Ki)
In vitro

Sanguinarine inhibited PP2C competitively with respect to α-casein (Ki=0.68 μM) and showed selectivity for PP2C as compared with PP1, PP2A, and PP2B in vitro. In vivo, sanguinarine showed cytotoxicity toward human promyelocytic leukemia cell line HL60, with an IC50 value of 0.37 μM, and induced apoptosis through a caspase-3/7-dependent mechanism involving the phosphorylation of p38, a PP2C substrate[1].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: HL60 cells
  • Concentrations: 1 μM
  • Incubation Time: 0-6 h
  • Method:

    HL60 cells (2×105 cells/ml) were treated with 1 μM sanguinarine for the indicated durations. Thereafter, cell lysates were assayed for caspase-3/7 activity using a chemiluminescent assay. kit.

  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 5 mg/mL
(13.59 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 367.78
Formula

C20H14NO4.Cl

CAS No. 5578-73-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C[N+]1=C2C(=C3C=CC4=C(C3=C1)OCO4)C=CC5=CC6=C(C=C52)OCO6.[Cl-]

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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