Catalog No.S1937

For research use only.

Isoniazid is a prodrug that blocks the action of fatty acid synthase by interacting with KatG , used for the prevention and treatment of tuberculosis.

Isoniazid Chemical Structure

CAS No. 54-85-3

Selleck's Isoniazid has been cited by 3 Publications

Purity & Quality Control

Choose Selective Fatty Acid Synthase Inhibitors

Biological Activity

Description Isoniazid is a prodrug that blocks the action of fatty acid synthase by interacting with KatG , used for the prevention and treatment of tuberculosis.
In vitro

Isoniazid is a prodrug that requires activation by the mycobacterial catalase-peroxidase enzyme (KatG) to an active form that then exerts a lethal effect on an intracellular target or targets. [1] Isoniazid upregulates the expression of an operon containing five FAS II components, including kasA and acpM. Isoniazid results in the accumulation of ACP-bound lipid precursors to mycolic acids that are 26 carbons long and fully saturated. [2] Isoniazid enters the mycobacterial cell by passive diffusion. Isoniazid itself is not toxic to the bacterial cell, but acts as a prodrug and is activated by the mycobacterial enzyme KatG, a multifunctional catalase-peroxidase that has other activities, including peroxynitritase and NADH oxidase. Isoniazid inhibits cell wall lipid synthesis, coupled with the findings that inhibitory INH adducts of NAD+/NADP+ are formed from the isonicotinoyl radical, leading the field away from this area. [3] Isoniazid induces a concentration-dependent (0-40 mM) cytotoxic effect in day-1 treated HepG2 cells and not significantly affected by decreases in intracellular GSH concentrations. [4]

In vivo Isoniazid increases CYP2E1 protein, and the 6-hydroxychlorzoxazone formation rate is increased by 2.7 and 2.2-fold in liver and kidney, respectively. Isoniazid decreases liver and kidney 20-HETE content to 34% and 15.6% of control, respectively, without significantly altering tissue 19-HETE concentration. [5]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 27 mg/mL
(196.87 mM)
Water 27 mg/mL
(196.87 mM)
Ethanol 27 mg/mL
(196.87 mM)

Chemical Information

Molecular Weight 137.14


CAS No. 54-85-3
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CN=CC=C1C(=O)NN

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT05117788 Not yet recruiting -- Tuberculosis Multidrug-Resistant Foundation for Innovative New Diagnostics Switzerland|V. N. Karazin Kharkiv National University|Kenya Medical Research Institute|Universidad Peruana Cayetano Heredia|Bioneer Corporation January 2022 --
NCT05124678 Not yet recruiting Drug: Isoniazid Tablets Tuberculosis Makerere University November 18 2021 Phase 2
NCT05073926 Recruiting Other: No intervention is part of the study protocol Latent Tuberculosis|Staphylococcus Aureus Region Skane September 30 2021 --
NCT04972903 Not yet recruiting -- Tuberculosis|Pulmonary Institut National de la Santé Et de la Recherche Médicale France|National Agency for Research on AIDS and Viral Hepatitis (ANRS) August 2021 --

(data from, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Isoniazid | Isoniazid ic50 | Isoniazid price | Isoniazid cost | Isoniazid solubility dmso | Isoniazid purchase | Isoniazid manufacturer | Isoniazid research buy | Isoniazid order | Isoniazid mouse | Isoniazid chemical structure | Isoniazid mw | Isoniazid molecular weight | Isoniazid datasheet | Isoniazid supplier | Isoniazid in vitro | Isoniazid cell line | Isoniazid concentration | Isoniazid nmr