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C75 trans Fatty Acid Synthase inhibitor

Name: C75 trans
Brand: Selleck Chemicals
SKU: S8915
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S8915

C75 trans (trans-C75, (±)-C75) is a novel, potent synthetic inhibitor of fatty acid synthase (FAS/FASN) with IC50 of 35 μM and 50 μM in clonogenic assays and spheroid growth assay, respectively. C75 is used as a tool for studying fatty acid synthesis in metabolic disorders and cancer.

Chemical Structure

Molecular Weight: 254.32

Jump to Mechanism of Action Cell Culture & Working Concentration Solubility Chemical Information, Storage & Stability Specificity

Quality Control

Batch: Purity: 99.93%

99.93

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Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
MA104	Antiviral assay	24 hrs	Antiviral activity against Rotavirus SA11 in MA104 cells assessed as inhibition of viral replication after 24 hrs by immunofluorescent assay and Western blotting analysis, ED50 = 21.2 μM.	22365411
MA104	Cytotoxicity assay	24 hrs	Cytotoxicity against monkey MA104 cells after 24 hrs, TD50 = 28.5 μM.	22365411
A375	Function assay		Inhibition of FASN in human A375 cells, IC50 = 32.43 μM.	21726077
KB	Cytotoxicity assay		Cytotoxicity in human KB cells, IC50 = 36.5 μM.	21504156
Click to View More Cell Line Experimental Data

Chemical Information, Storage & Stability

Molecular Weight	254.32	Formula	C₁₄H₂₂O₄	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	191282-48-1	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	trans-C75, (±)-C75			Smiles	CCCCCCCCC1C(C(=C)C(=O)O1)C(=O)O

Solubility

In vitro
Batch:

DMSO : 51 mg/mL (200.53 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 26 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.500mg/ml (9.83mM)	Taking the 1 mL working solution as an example, add 50 μL of 50 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.260mg/ml (4.95mM)	Taking the 1 mL working solution as an example, add 50 μL of 25.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	FASN ^[1] (clonogenic assay) 35 μM FASN ^[1] (spheroid growth assay) 50 μM
In vitro	C75 is toxic to prostate cancer cells. C75 reduces cell migration, while enhances radiation-induced clonogenic kill and enhances the spheroid growth delay induced by radiation exposure. Apoptosis induction by radiation exposure is enhanced by C75 treatment.^[1] C75 inhibits fatty acid synthesis and growth of established cancer cell lines from neu-N transgenic mice. C75 reduces the proliferation and expression of FAS, neu, and related genes in mammary epithelium.^[2]
In vivo	C75 inhibits fatty acid synthesis and in vivo mammary cancer development. C75 treatment prevents mammary cancer development and retards mammary development in neu-N transgenic mice.^[2]
References	[1] https://pubmed.ncbi.nlm.nih.gov/26484401/ [2] https://pubmed.ncbi.nlm.nih.gov/15489885/

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Handling Instructions

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