C75

C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid) , a more stable synthetic mimic of cerulenin, inhibits fatty-acid synthase (FAS/FASN) with an IC50 of 15.53 μM and has potential therapeutic effects in several cancer models. C75 is also a potent CPT1A activator.

C75 Chemical Structure

C75 Chemical Structure

CAS: 218137-86-1

Selleck's C75 has been cited by 2 publications

Purity & Quality Control

Batch: S981901 DMSO] 51 mg/mL] false] Ethanol] 51 mg/mL] false] Water] Insoluble] false Purity: 99.93%
99.93

C75 Related Products

Choose Selective Fatty Acid Synthase Inhibitors

Biological Activity

Description C75 (4-Methylene-2-octyl-5-oxotetrahydrofuran-3-carboxylic acid) , a more stable synthetic mimic of cerulenin, inhibits fatty-acid synthase (FAS/FASN) with an IC50 of 15.53 μM and has potential therapeutic effects in several cancer models. C75 is also a potent CPT1A activator.
Targets
FAS/FASN [1]
15.53 μM
In vitro
In vitro

C75, the competitive irreversible fatty acid synthase (FASN) inhibitor, possesses radiosensitizing activity in prostate cancer cell lines that is affected by the availability of fatty acids, also increases expression of fatty acid transporter proteins FATP1 and CD36.[3]

Cell Research Cell lines PC3, UVW and LNCaP cell lines, SK-N-BE(2c) and MCF7 cells
Concentrations 35 µM
Incubation Time 24 h
Method

Cells are seeded in 25 cm2 flasks at 105 cells/flask. When cultures are in exponential growth phase, medium is removed and replaced with fresh medium (including 1% or 10% serum) containing C75 (35 mM) and simultaneous administration of x-rays (2 Gy). Cells are then incubated for 24 h at 37℃ in 5% CO2. After treatment, cells are counted and seeded for clonogenic survival assay.  

In Vivo
In vivo

C75, an alpha-methylene-gamma-butyrolactone, reduces adipose tissue and fatty liver in treated DIO mice, despite high levels of malonyl-CoA.[4]

Animal Research Animal Models Diet-induced obese mice model
Dosages 20, 15, 10, 15, 15 mg/kg
Administration i.p.

Chemical Information & Solubility

Molecular Weight 254.32 Formula

C14H22O4

CAS No. 218137-86-1 SDF --
Smiles CCCCCCCCC1OC(=O)C(=C)C1C(O)=O
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 51 mg/mL ( (200.53 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 51 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy C75 | C75 supplier | purchase C75 | C75 cost | C75 manufacturer | order C75 | C75 distributor