BI 2536

For research use only.

Catalog No.S1109

249 publications

BI 2536 Chemical Structure

CAS No. 755038-02-9

BI-2536 is a potent Plk1 inhibitor with IC50 of 0.83 nM in a cell-free assay. BI-2536 inhibits Bromodomain 4 (BRD4) with Kd of 37 nM and potently suppresses c-Myc expression. BI-2536 induces apoptosis and attenuates autophagy. Phase 2.

Selleck's BI 2536 has been cited by 249 publications

Purity & Quality Control

Choose Selective PLK Inhibitors

Biological Activity

Description BI-2536 is a potent Plk1 inhibitor with IC50 of 0.83 nM in a cell-free assay. BI-2536 inhibits Bromodomain 4 (BRD4) with Kd of 37 nM and potently suppresses c-Myc expression. BI-2536 induces apoptosis and attenuates autophagy. Phase 2.
Features The first potent and selective Plk1 inhibitor that induces all hallmarks of Plk1 inhibition.
Targets
c-Myc [3]
()
PLK1 [1]
(Cell-free assay)
PLK2 [1]
(Cell-free assay)
PLK3 [1]
(Cell-free assay)
BRD4 [3]
(Cell-free assay)
0.83 nM 3.5 nM 9.0 nM 37 nM(Kd)
In vitro

BI 2536 blocks the activities of Plk2 and Plk3 to a slightly lesser extent with IC50 of 3.5 nM and 9.0 nM, respectively. In HeLa cells, BI 2536 treatment ranging from 10-100 nM leads to the blocking of the recruitment of γ-tubulin and phosphorylation of Apc6 at mitotic centrosomes, inhibition of cohesin release from chromosome arms, induction of monopolar spindles, as well as a range of other mitotic processes that are known to depend on Plk1. BI 2536 treatment leads to the HeLa cells arrested in G2/M, subsequently a sub-G1 DNA peak indicative of DNA breakdown and apoptosis, and accumulated cleaved PARP p85 fragments in a concentration-dependent manner. BI 2536 inhibits the growth of a panel of 32 human cancer cell lines with EC50 of 2-25 nM, while blocking the proliferation of exponentially growing hTERT-RPE1, human umbilical vein endothelial cells (HUVECs), and normal rat kidney (NRK) cells with EC50 of 12-31 nM. [1] Plk1 inhibition by BI 2536 reduces the growth and viability of anaplastic thyroid carcinoma (ATC) cells such as CAL62, OCUT-1, SW1736, 8505C, and ACT-1 with EC50 values of 1.4-5.6 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PSN1 M1HMe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MofqTWM2OD1yLkCwO|c3KM7:TR?= NW\VbpBzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
ACN MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTtTWM2OD1yLkCwPFQ{KM7:TR?= NVvkTmc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
TE-8 M2r4RWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NU\3TWZTUUN3ME2wMlAxQTRzIN88US=> NY[zV4lNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
LC-2-ad NXOzPIZ{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\lNmx1UUN3ME2wMlAyODl|IN88US=> NH3xSpQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
ALL-PO MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{f5UmlEPTB;MD6wNVMh|ryP MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
EW-16 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfoTWM2OD1yLkCxN|M3KM7:TR?= NGrPWos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
KM12 M2jtZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXrYd203UUN3ME2wMlAyOzR6IN88US=> NHnaSFE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
GI-1 M3;mWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1\ydmlEPTB;MD6wNVM5OyEQvF2= NInydZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
LS-411N MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTBwMEG0NFch|ryP M3zldlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
ES8 Mlf4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3\FRWlEPTB;MD6wNVQ6PCEQvF2= NEHtUGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SNU-C2B NYnKd4k6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIHtOGVKSzVyPUCuNFE2OjZizszN MoqxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CESS MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXzJR|UxRTBwMEG2NFIh|ryP MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
697 NGrrWW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2i5WGlEPTB;MD6wNVY3QCEQvF2= NFO3W3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1963 MnfyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXS2eGlXUUN3ME2wMlAyPjd|IN88US=> NH3nOpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
IA-LM M4foeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPsd|RKSzVyPUCuNFE3QDhizszN M1vNelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
SW954 NU\NNnFvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEHoN45KSzVyPUCuNFE4ODVizszN NFzte|U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
RL95-2 M4XYXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXTJR|UxRTBwMEG3N|gh|ryP MkDRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
LXF-289 NV;zTGRVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzBVoNWUUN3ME2wMlAyPzdizszN MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
OCUB-M NGX2dZNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nBdmlEPTB;MD6wNVgzPiEQvF2= MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
RKO M3nPZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fpPGlEPTB;MD6wNVk5PyEQvF2= NFr3[IY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
LOUCY NH\jdohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PtOWlEPTB;MD6wNlA2KM7:TR?= M1TxXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NOS-1 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\CTWM2OD1yLkCyNFk{KM7:TR?= Mmm4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
A4-Fuk MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTBwMEKyNVgh|ryP NUW4[oV4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
A388 MmD3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjxTWM2OD1yLkCyNlI4KM7:TR?= NVe1RW1pRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
TE-9 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlvnTWM2OD1yLkCyNlYyKM7:TR?= M{XSVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
KURAMOCHI M1zwOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2n2XmlEPTB;MD6wNlI5PSEQvF2= NFjBOpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
KALS-1 NGLJfHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVTJR|UxRTBwMEKzN|gh|ryP NXrGTZYxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
HOP-62 NHSzOG5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2TIZmlEPTB;MD6wNlQzKM7:TR?= MkGxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
RPMI-8402 M1fSN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2nRb2lEPTB;MD6wNlUxQCEQvF2= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
TE-1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHPqSnZKSzVyPUCuNFI2OTJizszN NY\nd|BvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
CCRF-CEM MoThS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnv1TWM2OD1yLkCyOVQ{KM7:TR?= NHy3cVc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HUTU-80 NFziUY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVLWS2xiUUN3ME2wMlAzPjJ2IN88US=> NF\5SmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1648 NGrmUGxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{PZOmlEPTB;MD6wNlY1PyEQvF2= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
HCE-4 M4PGfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHnEU3dKSzVyPUCuNFI3QDJizszN NVLkboNQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
ETK-1 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnxXXNGUUN3ME2wMlAzPzN4IN88US=> NWDjRZFORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
TE-15 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmDaTWM2OD1yLkCyO|M5KM7:TR?= MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
BE-13 NUnwOHlQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrqfXZKSzVyPUCuNFI4PCEQvF2= NF3Y[mo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
KE-37 NUHPXWxzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGXQbm5KSzVyPUCuNFI4PDFizszN MlKzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H1355 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVHSPXhxUUN3ME2wMlAzPzh|IN88US=> M3Pt[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
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GT3TKB NVu5NYhLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1nwSGlEPTB;MD6wNlk2OyEQvF2= NHTSToo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
LB647-SCLC MljlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTBwMEOxPVUh|ryP MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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LB2241-RCC NIDEWY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVvJR|UxRTBwNUK1NlUh|ryP MmLrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H1417 M3;1[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYHJR|UxRTBwNUO0N|Uh|ryP MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H2081 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUTWOYZnUUN3ME2wMlU5PjFzIN88US=> NHPob4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1522 NFfzb29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHMSnFlUUN3ME2wMlU6Ozh|IN88US=> M3fXRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H889 MnPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHzpTnpKSzVyPUCuOVk5PDRizszN Mm\QQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CGTH-W-1 MkLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfWZ|dEUUN3ME2wMlYxPzV3IN88US=> M3TSdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
EB-3 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWX1WWtZUUN3ME2wMlYyOTV|IN88US=> MonsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
D-336MG MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX\lfo1FUUN3ME2wMlYyPzlzIN88US=> M4fve|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LC-1F NVfLc|B5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXrJR|UxRTBwNkK1PVIh|ryP NELqb2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
MHH-NB-11 MormS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\JZZlKSzVyPUCuOlM5ODFizszN NYTwcZhJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
SK-N-FI M{Xrb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4rMemlEPTB;MD62N|kyKM7:TR?= M{D5U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H69 M4\HN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TPZWlEPTB;MD62OFg4QSEQvF2= NFPGdJk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SCH MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFTIZnJKSzVyPUCuOlYxOjNizszN NVXwT2dURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
LS-513 NITqNXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mnz4TWM2OD1yLk[3NlU6KM7:TR?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
CP67-MEL MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37idWlEPTB;MD62O|U6PiEQvF2= NH:2Z2o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
GCIY NITrT2RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rofGlEPTB;MD63NFM3OyEQvF2= M3y1flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
SHP-77 NYLKc41wT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjzNXdKSzVyPUCuO|A4OzRizszN MkXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
EHEB MkPxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml65TWM2OD1yLkexPFU1KM7:TR?= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
D-247MG MlLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmjLTWM2OD1yLkezNlMzKM7:TR?= NUfLNIhURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
Raji Mnf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHLre4lKSzVyPUCuO|Q6QDlizszN M3vOWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
MPP-89 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfXTWM2OD1yLke1PVc5KM7:TR?= M3zJRlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
EKVX NYLSSVZXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWLJR|UxRTBwN{mwO|Mh|ryP M4fvelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
HCC2218 M3TieGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYmzN|VnUUN3ME2wMlc6OTNzIN88US=> MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
KNS-81-FD NX7EeFhtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUnJR|UxRTBwOEGwNVEh|ryP MofPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
HCC2157 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEfaSpRKSzVyPUCuPFQ6PTFizszN NHfTSnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
BT-474 MlO2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPnO5V6UUN3ME2wMlg5QDV2IN88US=> M1HzdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H748 NUHCTYFJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTOTWM2OD1yLkmxNkDPxE1? MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
EW-24 MlnFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHIR45KSzVyPUCuPVIxODJizszN NXTESI5qRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
D-263MG MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGe0ZXlKSzVyPUCuPVQ2OTJizszN MlrvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
OMC-1 MnPDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHoUnVwUUN3ME2wMlk3Ozd|IN88US=> MmXSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H2196 MnS1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mny2TWM2OD1yLkm3O|c4KM7:TR?= M{n2RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
HAL-01 NGfKSYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHLLUHpKSzVyPUCuPVk5PzJizszN M33rblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H2107 NVfxXG1kT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1f6R2lEPTB;MT6wNVg2PCEQvF2= MnLMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
LB996-RCC NVridmxxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;leYo5UUN3ME2xMlA5ODVzIN88US=> NGL5N3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H1395 NFHn[odIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfYd5Z[UUN3ME2xMlExPjdizszN MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
CHP-126 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\EVHFuUUN3ME2xMlE3OTl7IN88US=> MlfCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NB69 MlrUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWnJR|UxRTFwMk[xOFgh|ryP M2DD[lxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
CAS-1 NF7GZ5BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPifZRKSzVyPUGuN|QzOzFizszN M3zrd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
JVM-3 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjJTWM2OD1zLkS3PFU2KM7:TR?= NWLPVJVERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
DMS-153 NVj4e3NyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjBTWM2OD1zLkW3Olc1KM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
SK-MEL-2 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHvGeFBKSzVyPUGuO|E4QDlizszN MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H1155 NYDZWWt1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnD[FZsUUN3ME2xMlc{Pjd4IN88US=> M1W3XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H1092 MlnaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnXZTWM2OD1zLke2NVgzKM7:TR?= M13VcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LU-165 NU\TZ3lMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVv6S|I4UUN3ME2xMlg3PDd4IN88US=> NVnadJg2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
HL-60 NWLXUJdnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVnJR|UxRTFwOUm5NFgh|ryP MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
REH NHXJ[pBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmHETWM2OD1{LkCwOVgzKM7:TR?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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RS 4;11 NFnPN|dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF[3bJIzPCCq NHjaT4NKSzVyPUWxPU4xPSCwTR?= M4nkO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
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SIL-ALL NFXuWZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPzNlQhcA>? MoLJTWM2OD1zOUmuO|chdk1? M{O4d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
CEM MoXYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUKyOEBp MUHJR|UxRTV{LkG4JI5O M{\jXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
P12 M2exNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWnTTXA5OjRiaB?= MmHXTWM2OD1|OT6yO{BvVQ>? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
NALM-16 M2DCfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PZeVI1KGh? NWXSWGZ2UUN3ME25Ok4zQCCwTR?= NILKNXI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
JURKAT MlPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITMZWI1QCCq NYrzeFY{UUN3ME2xOE45PCCwTR?= MkXkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
MOLT-4 M2jKcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILPV5c1QCCq MljLTWM2OD1{Nj62JI5O M1jYXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
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NALM-6 MmXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlv5OFghcA>? NG\ZUHZKSzVyPUKzMlI3KG6P MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
RS 4;11 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXG0PEBp MX\JR|UxRTF4ND6yPEBvVQ>? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
697 NVLC[5RrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIX5SZc1QCCq MV3JR|UxRTNyNT6zJI5O MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
SIL-ALL MnPBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4K5elQ5KGh? NVLHN4F7UUN3ME23OE43OiCwTR?= NX\4WnFKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
CEM MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXXX[mw{PDhiaB?= M2ThRmlEPTB;MkSuOVUhdk1? MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
P12 NEDwWWlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnmyOFghcA>? NYTBcY9OUUN3ME2xPE41PiCwTR?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
NALM-16 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4\I[FQ5KGh? MVPJR|UxRTZ|LkK0JI5O MmrMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
JURKAT MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fneFczKGh? NUfiVVFPUUN3ME2zMlY2KG6P NHPXTWY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
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SIL-ALL MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUT3OJdwPzJiaB?= NFzPSZBKSzVyPUeuNkBvVQ>? NV3WcFRXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
CEM M2Th[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWW3NkBp M2jsXWlEPTB;NT60NkBvVQ>? NXu1PHhiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
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OHS-50 Mk\1dWhVWyCjc4PhfS=> M1rHbZFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJG9JWy13MDDj[Yxtew>? M2r1dlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
RD NYfEV5BJeUiWUzDhd5NigQ>? M3H3O5FJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KEOxbn\pdo1ifG:{eTDzZ5Jm\W5iZn;yJHJFKGOnbHzz NInlRXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
SJ-GBM2 MnnOdWhVWyCjc4PhfS=> M3fObpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCVSj3HRm0zKGOnbHzz MkD2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjl2M{WxN|koRjJ7NEO1NVM6RC:jPh?=
SK-N-MC MYDxTHRUKGG|c3H5 NED4OolyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiU1utUk1OSyClZXzsdy=> M2DIW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
NB-EBc1 MnPKdWhVWyCjc4PhfS=> NInrOHhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKtSWJkOSClZXzsdy=> NHTmcXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
LAN-5 MkCzdWhVWyCjc4PhfS=> M4HhZpFJXFNib3[gdIVlcWG2cnnjJINidmOncjDj[YxtKGyrbnXzJJRwKGmmZX70bYZ6KG23bITpdIxmKG:ycH;yeJVvcXSrZYOg[o9zKGS{dXegdoVxfXKyb4Ppcoc7KFC{aX3hdpkhe2O{ZXXuJIZweiCOQV6tOUBk\Wyucx?= M{HwV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Rh18 NYXKPI04eUiWUzDhd5NigQ>? NG[wTY9yUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiUnixPEBk\Wyucx?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
NB1643 NX3TdXhUeUiWUzDhd5NigQ>? NUnDcJVqeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhVkJzNkSzJINmdGy| M{fGPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SK-N-MC MlK0dWhVWyCjc4PhfS=> NFi0NHdyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUy2QLV3DJINmdGy| M13mSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
SJ-GBM2 MWPxTHRUKGG|c3H5 NXTrcJJ1eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW0pvR1LNNkBk\Wyucx?= MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 MWTxTHRUKGG|c3H5 NVvkPIhZeUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhXEN|MjDj[Yxtew>? MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
Rh18 NWT6[Jh4eUiWUzDhd5NigQ>? MnHldWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgVogyQCClZXzsdy=> M1jaZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
Saos-2 MmDZdWhVWyCjc4PhfS=> NV;JTHp4eUiWUzDv[kBx\WSrYYTybYMh[2GwY3XyJINmdGxibHnu[ZMhfG9iaXTlcpRq\nlibYXseIlxdGVib4Dwc5J1fW6rdHnld{Bnd3JiZIL1[{Bz\XC3coDvd4lv\zpiQ3;u[olzdWG2b4L5JJNkemWnbjDmc5IhW2Gxcz2yJINmdGy| M3LEe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ7NEO1NVM6Lz5{OUSzOVE{QTxxYU6=
DU145 MojrRZBweHSxc3nzJIF{e2G7 MUGxNFAhdk1? NWHtcW5vOjRiaILz M2DIdmlv\HWldHnvckBw\iCjcH;weI9{cXNiaX6gbJVu[W5iRGWxOFUh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iUFHSVEBkdGWjdnHn[UBifCBzMECgcm0h[W[2ZYKgNlQhcHK|IHL5JIludXWwb3Lsc5R1cW6pIH3leIhw\A>? M1zEbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nki1PVQyLz5{NU[4OVk1OTxxYU6=
HeLa NWrMbGdpTnWwY4Tpc44h[XO|YYm= MYGxNFAhdk1? Ml7JTY5pcWKrdHnvckBw\iCSTFuxJHRpejJzMDDwbI9{eGixconsZZRqd25iaX6gbJVu[W5iSHXMZUBk\WyuczDheEAyODBibl2gZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:m M2LmO|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nki1PVQyLz5{NU[4OVk1OTxxYU6=
DU145 NIj3OGpHfW6ldHnvckBie3OjeR?= NFLuT5cyODBibl2= M3zqTGlvcGmkaYTpc44hd2ZiUFzLNUBxem:2ZXnuJIFk[3WvdXzheIlwdiCrbjDoeY1idiCGVUG0OUBk\WyuczDheEAyODBibl2gZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:m Mn;4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4OEW5OFEoRjJ3Nki1PVQyRC:jPh?=
HeLa MV;GeY5kfGmxbjDhd5NigQ>? NVv3WJZUOTByIH7N MX7Jcohq[mm2aX;uJI9nKFCOS{GgdJJwfGWrbjDhZ4N2dXWuYYTpc44hcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCxNFAhdk1iYomgbY1ufW6xYnzveJRqdmdibXX0bI9l MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
HeLa M2q2VmZ2dmO2aX;uJIF{e2G7 NUH5[HpUOTByIH7N M374OlE5KGi{cx?= MWXJcoR2[3Srb36gc4YhdW:wb4DvcIFzKG2rdH;0bYMhe3CrbnTs[ZMhcW5iaIXtZY4hUGWOYTDj[YxteyCjdDCxNFAhdk1iaX7jeYJifGWmIH\vdkAyQCCqcoOgZpkhcW6maYLlZ5QhcW2vdX7v[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHl? NUXVS4dURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFU6PDFpPkK1Olg2QTRzPD;hQi=>
HeLa MYjBdI9xfG:|aYOgZZN{[Xl? MmXzNVAxKG6P M4\YclI1KGi{cx?= NEDFbG1KdmS3Y4Tpc44hd2ZiYYDvdJRwe2m|IHnuJIh2dWGwIFjlUIEh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iUFHSVEBkdGWjdnHn[UBifCBzMECgcm0h[W[2ZYKgNlQhcHK|IHL5JIludXWwb3Lsc5R1cW6pIH3leIhw\A>? M4HMZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ3Nki1PVQyLz5{NU[4OVk1OTxxYU6=
PC3 NHf6VGdHfW6ldHnvckBie3OjeR?= NWjMS3Z1OTByIH7N MoTrNVghcHK| Mo\rTY5lfWO2aX;uJI9nKG2xbn;wc4xieiCvaYTveIlkKHOyaX7kcIV{KGmwIHj1cYFvKFCFMzDj[YxteyCjdDCxNFAhdk1iaX7jeYJifGWmIH\vdkAyQCCqcoOgZpkhcW6maYLlZ5QhcW2vdX7v[ox2d3Knc3PlcoNmKG2rY4Lvd4NweHl? NVLKflc{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFU6PDFpPkK1Olg2QTRzPD;hQi=>
DU145 NF7PO41HfW6ldHnvckBie3OjeR?= NGrLO3cyODBibl2= MXTJcohq[mm2aX;uJI9nKFCOS{GgWIhzOjFyIIDoc5NxcG:{eXzheIlwdiCrbjDoeY1idiCGVUG0OUBk\WyuczDheEAyODBibl2gZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:m NWq1d2s4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFU6PDFpPkK1Olg2QTRzPD;hQi=>

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
pro-caspase-8 / cleaved caspase-8 / cleaved caspase-3 / cleaved caspase-9; 

PubMed: 27699933     


PLKi‐induced caspase activation. HeLa cells were treated with BI 2536 (10 nmol/L), BI 6727 (68 nmol/L), or GSK461364 (11 nmol/L), and HCT 116 cells were treated with BI 2536 (20 nmol/L), BI 6727 (70 nmol/L), or GSK461364 (12 nmol/L) for 48 h. The levels of cleaved caspase (CASP)‐3, ‐8, and ‐9 were examined by Western blot analysis.

p-HH3 / PARP / Myc / p-Myc; 

PubMed: 24771642     


Western blotting showing more mitotic arrested (shown by phospho-histone H3) and apoptotic (shown by PARP) cells as well as lower MYC levels in LNCaP-Pim1 cells after BI 2536 treatment compared with LNCaP-Neo cells. The difference is obvious at lower dose of BI 2536 (10 nM). Both total MYC expression and phosphor-MYC levels were lower in Pim1 cells than Neo cells after BI 2536 treatment.

pMEK / MEK / pERK / ERK / pRSK1 / RSK1; 

PubMed: 28430578     


(A) and (B) Western blot analysis of the expression and activation of components of the MEK-ERK-RSK1 pathway in MKN45 and MKN74 cancer cells treated with BI 2536 and BI 6727 for 24 and 48 hours, respectively.

pPlk1 / Plk1 / pMyt1; 

PubMed: 28430578     


(A) and (B) Western blot analysis of the expression and phosphorylation of Plk1, and phosphorylation of Myt1 in the CSC-like and cancer cells after treatment with BI 2536 and BI 6727 for 24 and 48 hours, respectively.

Aurora B / pAurora B / pAurora C / pH3 / Cyclin B1 / Securin / pBcl-XL / MPM2; 

PubMed: 25169932     


Western blot analysis of cells treated for 24 h with the indicated inhibitor concentrations. Cell lysates were immunoblotted for Plk1, Phospho-Aurora B (T232)/Aurora C (T198), Aurora B, histone H3 phosphorylated at S10 (pH3), Cdk1, Cyclin B1, Securin, Phospho-Bcl-XL, Induced myeloid leukemia cell differentiation protein Mcl-1, MPM-2 and β-Actin. Left (Hela cells); Right (HUVEC)

27699933 24771642 28430578 25169932
Immunofluorescence
p-H2AX / propidium iodide; 

PubMed: 22309939     


Apoptotic images of the cells after BI 2536 treatment, as revealed by HCS (high-content screening) instrument (×10) with phospho-H2AX antibody or PI (propidium iodide) intake. 

Pericentrin / α-tubulin; 

PubMed: 21772266     


MEFs were labelled using Plk1, pericentrin and α-tubulin antibodies and 4,6-diamidino-2-phenylindole for the analysis by immunofluorescence microscopy. Representative examples of BI 2536-treated and control MEFs are depicted. Scale bar, 5 μm.

22309939 21772266
Growth inhibition assay
Cell viability; 

PubMed: 21772266     


Proliferative activity of MEFs at different BI 2536 concentrations (25–250 nM; means±s.d., n=3, for each concentration). *P<0.05, **P<0.01, ***P<0.001, Student's t-test, unpaired and two-tailed.

21772266
In vivo

BI 2536 given i.v. once or twice per week is highly efficacious in diverse xenograft models with acceptable tolerability by inhibiting cell proliferation through a mitotic arrest, and subsequently induction of tumor-cell death. Administration of BI 2536 at 50 mg/kg once or twice per week significantly inhibits growth of HCT 116 xenografts with T/C of 15% and 0.3%, respectively. BI 2536 treatment twice-weekly also leads to excellent tumor-growth in BxPC-3 and A549 models with T/C of 5% and 14%, respectively. [1]

Protocol

Kinase Assay:

[1]

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Plk1 in vitro kinase assay:

Recombinant human Plk1 (residues 1-603) is expressed as N-terminal, GST-tagged fusion protein with a baculoviral expression system and purified by affinity chromatography with Glutathione-agarose. Enzyme activity assays for Plk1 are performed in the presence of serially diluted BI 2536 with 20 ng of recombinant kinase and 10 μg casein from bovine milk as the substrate. Kinase reactions are performed in a final volume of 60 μL for 45 minutes at 30 °C (15 mM MgCl2, 25 mM MOPS [pH 7.0], 1 mM DTT, 1% DMSO, 7.5 mM ATP, 0.3 μCi γ-33P-ATP). Reactions are terminated by the addition of 125 μL of ice-cold 5% TCA. After transfer of the precipitates to Multi-Screen mixed ester cellulose filter plates, plates are washed with 1% TCA and quantified radiometrically. Dose-response curve is used for calculating IC50 value.
Cell Research:

[1]

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  • Cell lines: HeLa, A43, SKOV-3, HT-29, K562, A549, Saos-2, MCF7, HCT116, COLO 205, Hep G2, Raji and PC-3 cells, etc.
  • Concentrations: Dissolved in DMSO, final concentrations ~1 μM
  • Incubation Time: 24 and 72 hours
  • Method:

    Cells are exposed to various concentrations of BI 2536 for 24, and 72 hours. Cell growth is assessed by the measurement of Alamar Blue dye conversion in a fluorescence spectrophotometer. For determining the DNA content of the cultures, cell suspensions are fixed in 80% ethanol, treated for 5 minutes with 0.25% Triton X-100 in PBS, and incubated with 0.1% RNase and 10 μg/mL propidium iodide (PI) in PBS for 20 minutes at RT. Cell-cycle profiles are determined by flow cytometric analysis.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Female BomTac:NMRI-Foxn1nu mice injected subcutaneously with HCT 116, NCI-H460, or A549 cells
  • Dosages: ~50 mg/kg
  • Administration: Injection i.v. once or twice per week
    (Only for Reference)

Solubility (25°C)

In vitro Ethanol 100 mg/mL (191.69 mM)
DMSO 21 mg/mL (40.25 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
For best results, use promptly after mixing.
12.5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 521.66
Formula

C28H39N7O3

CAS No. 755038-02-9
Storage powder
in solvent
Synonyms N/A
Smiles CCC1C(=O)N(C2=CN=C(N=C2N1C3CCCC3)NC4=C(C=C(C=C4)C(=O)NC5CCN(CC5)C)OC)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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% DMSO % % Tween 80 % ddH2O
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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02215044 Terminated Drug: BI 2536 intravenous|Drug: Gemcitabine intravenous Pancreatic Neoplasms Boehringer Ingelheim June 2007 Phase 1
NCT02211833 Completed Drug: BI 2536|Drug: Pemetrexed Carcinoma Non-Small-Cell Lung Boehringer Ingelheim October 2006 Phase 1
NCT00701766 Completed Drug: BI 2536 Leukemia Myeloid Acute Boehringer Ingelheim October 2006 Phase 2
NCT00706498 Completed Drug: BI 2536 Prostatic Neoplasms Boehringer Ingelheim September 2006 Phase 2
NCT00376623 Completed Drug: BI 2536 Carcinoma Non-Small-Cell Lung Boehringer Ingelheim July 2006 Phase 2
NCT02211872 Completed Drug: BI 2536 BS intravenous Neoplasms Boehringer Ingelheim August 2004 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    We need to use BI 2536 in preclinical studies using mice, how to formulate the inhibitor for this in vivo study?

  • Answer:

    For in vivo study, DMSO should not be more than 5% because the toxicity. You can formulated BI2536 in hydrochloric acid (0.1 N), and diluted with 0.9% NaCl for animal study according to the reference :( Supplemental Data) http://linkinghub.elsevier.com/retrieve/pii/S0960-9822(06)02671-6

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID