BI 2536

Catalog No.S1109

For research use only.

BI-2536 is a potent Plk1 inhibitor with IC50 of 0.83 nM in a cell-free assay. BI-2536 inhibits Bromodomain 4 (BRD4) with Kd of 37 nM and potently suppresses c-Myc expression. BI-2536 induces apoptosis and attenuates autophagy. Phase 2.

BI 2536 Chemical Structure

CAS No. 755038-02-9

Selleck's BI 2536 has been cited by 261 publications

Purity & Quality Control

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Biological Activity

Description BI-2536 is a potent Plk1 inhibitor with IC50 of 0.83 nM in a cell-free assay. BI-2536 inhibits Bromodomain 4 (BRD4) with Kd of 37 nM and potently suppresses c-Myc expression. BI-2536 induces apoptosis and attenuates autophagy. Phase 2.
Features The first potent and selective Plk1 inhibitor that induces all hallmarks of Plk1 inhibition.
Targets
c-Myc [3] PLK1 [1]
(Cell-free assay)
PLK2 [1]
(Cell-free assay)
PLK3 [1]
(Cell-free assay)
BRD4 [3]
(Cell-free assay)
0.83 nM 3.5 nM 9.0 nM 37 nM(Kd)
In vitro

BI 2536 blocks the activities of Plk2 and Plk3 to a slightly lesser extent with IC50 of 3.5 nM and 9.0 nM, respectively. In HeLa cells, BI 2536 treatment ranging from 10-100 nM leads to the blocking of the recruitment of γ-tubulin and phosphorylation of Apc6 at mitotic centrosomes, inhibition of cohesin release from chromosome arms, induction of monopolar spindles, as well as a range of other mitotic processes that are known to depend on Plk1. BI 2536 treatment leads to the HeLa cells arrested in G2/M, subsequently a sub-G1 DNA peak indicative of DNA breakdown and apoptosis, and accumulated cleaved PARP p85 fragments in a concentration-dependent manner. BI 2536 inhibits the growth of a panel of 32 human cancer cell lines with EC50 of 2-25 nM, while blocking the proliferation of exponentially growing hTERT-RPE1, human umbilical vein endothelial cells (HUVECs), and normal rat kidney (NRK) cells with EC50 of 12-31 nM. [1] Plk1 inhibition by BI 2536 reduces the growth and viability of anaplastic thyroid carcinoma (ATC) cells such as CAL62, OCUT-1, SW1736, 8505C, and ACT-1 with EC50 values of 1.4-5.6 nM. [2]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
PSN1 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfxTWM2OD1yLkCwO|c3KM7:TR?= MmLPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
ACN NWTuXJBuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXTJR|UxRTBwMEC4OFMh|ryP NFLBcoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
TE-8 NW\LUmNRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVuxV4kxUUN3ME2wMlAxQTRzIN88US=> MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
LC-2-ad NH61SWhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWj0V5hMUUN3ME2wMlAyODl|IN88US=> M4jLOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
ALL-PO MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLKemxOUUN3ME2wMlAyOyEQvF2= MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
EW-16 M{jXO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVrJR|UxRTBwMEGzN|Yh|ryP M2[5SVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
KM12 NFPUemxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLiRmtKUUN3ME2wMlAyOzR6IN88US=> MkjpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
GI-1 NGfRXpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn\CTWM2OD1yLkCxN|g{KM7:TR?= M1HzTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LS-411N MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17vZWlEPTB;MD6wNVQxPyEQvF2= NFnK[pk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
ES8 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXHJR|UxRTBwMEG0PVQh|ryP NF;VbpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
SNU-C2B NY\aN2ZjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M36wV2lEPTB;MD6wNVUzPiEQvF2= NH3TSHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
CESS NEj1eGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHHHVWFKSzVyPUCuNFE3ODJizszN NF23TVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
697 NW\aXlNtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH31U3pKSzVyPUCuNFE3PjhizszN MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H1963 M2P5XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mnq4TWM2OD1yLkCxOlc{KM7:TR?= MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
IA-LM MoLBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoXJTWM2OD1yLkCxOlg5KM7:TR?= MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
SW954 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVvTdZc3UUN3ME2wMlAyPzB3IN88US=> M1\wclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
RL95-2 MlL6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXy3c41KUUN3ME2wMlAyPzN6IN88US=> M{Dsd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
LXF-289 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrzXFlVUUN3ME2wMlAyPzdizszN MlvVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
OCUB-M Mnq3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXHJR|UxRTBwMEG4NlYh|ryP NEDIS4U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
RKO Mn[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUjJR|UxRTBwMEG5PFch|ryP NFPTdmQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
LOUCY MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX71XWJEUUN3ME2wMlAzODVizszN M1P2TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NOS-1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDmW2xKSzVyPUCuNFIxQTNizszN MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
A4-Fuk NVXIc|lIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmr5TWM2OD1yLkCyNlE5KM7:TR?= MlTEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
A388 MlnJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofDTWM2OD1yLkCyNlI4KM7:TR?= M2PabVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
TE-9 MmTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLUTWM2OD1yLkCyNlYyKM7:TR?= NUHBUmFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
KURAMOCHI MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\qOJpKSzVyPUCuNFIzQDVizszN NGfyUGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
KALS-1 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVvJR|UxRTBwMEKzN|gh|ryP NEPQcHQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HOP-62 NYLvN|drT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYjJR|UxRTBwMEK0NkDPxE1? MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
RPMI-8402 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYXJR|UxRTBwMEK1NFgh|ryP NG\XTY49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
TE-1 NEXae3VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LnZmlEPTB;MD6wNlUyOiEQvF2= MmrDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CCRF-CEM MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXsTWM2OD1yLkCyOVQ{KM7:TR?= NEjWN|Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HUTU-80 NWLLT4lkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYPJR|UxRTBwMEK2NlQh|ryP M1;4VFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H1648 NVHucoZlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjhWmZKSzVyPUCuNFI3PDdizszN NGLpc2E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HCE-4 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnmxTWM2OD1yLkCyOlgzKM7:TR?= NIj3SFI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
ETK-1 NV3sR2FnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWqwRVBNUUN3ME2wMlAzPzN4IN88US=> M2q3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
TE-15 NGS2coxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfOWZlKSzVyPUCuNFI4OzhizszN M{P2clxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
BE-13 NXWwT29RT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTBwMEK3OEDPxE1? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
KE-37 NVrlbo1KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHjDXWxKSzVyPUCuNFI4PDFizszN NUTseYRKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H1355 MlzjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{f2VGlEPTB;MD6wNlc5OyEQvF2= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
TE-11 M3WxV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUXsUpZnUUN3ME2wMlAzQDB7IN88US=> NIHzNpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
BL-70 NXOwbnRbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkDsTWM2OD1yLkCyPFU4KM7:TR?= M2\VeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
ES6 NVT4SW9FT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXzzUGFqUUN3ME2wMlAzQDhzIN88US=> NFTGWVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
GT3TKB MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTBwMEK5OVMh|ryP MnT0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
LB647-SCLC NIDzVGNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlziTWM2OD1yLkCzNVk2KM7:TR?= MnjuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
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LB2241-RCC MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmK5TWM2OD1yLkWyOVI2KM7:TR?= M3:zc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H1417 MoL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYjRNGNwUUN3ME2wMlU{PDN3IN88US=> NETIZpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H2081 M3TkSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrOWWxnUUN3ME2wMlU5PjFzIN88US=> NUD0N5ZVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
NCI-H1522 NYKzUnhST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWr0N5BrUUN3ME2wMlU6Ozh|IN88US=> NHLiZmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H889 Ml7SS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTBwNUm4OFQh|ryP M3TTXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
CGTH-W-1 NXzodZFZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlL2TWM2OD1yLk[wO|U2KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
EB-3 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGD3UpFKSzVyPUCuOlEyPTNizszN NFu2SGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
D-336MG Mn3hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;PTWM2OD1yLk[xO|kyKM7:TR?= MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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MHH-NB-11 NFSw[I1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIXOeohKSzVyPUCuOlM5ODFizszN MnXQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
SK-N-FI MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M163eGlEPTB;MD62N|kyKM7:TR?= MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
NCI-H69 NFzZOoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mmm1TWM2OD1yLk[0PFc6KM7:TR?= M3;HdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
SCH M1HSUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEjSRVRKSzVyPUCuOlYxOjNizszN MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
LS-513 Mkj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmr1TWM2OD1yLk[3NlU6KM7:TR?= MoLIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
CP67-MEL Moq1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4DrdmlEPTB;MD62O|U6PiEQvF2= NEPaOWs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
GCIY NGnXcmRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NE\ye2JKSzVyPUCuO|A{PjNizszN MkHuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
SHP-77 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWnLTmxNUUN3ME2wMlcxPzN2IN88US=> M3T6WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
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D-247MG MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlK0TWM2OD1yLkezNlMzKM7:TR?= NVftcVhXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
Raji MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXG1O5huUUN3ME2wMlc1QTh7IN88US=> NFL4VoM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
MPP-89 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoXFTWM2OD1yLke1PVc5KM7:TR?= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
EKVX MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnTLTWM2OD1yLke5NFc{KM7:TR?= NX7sS|E{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
HCC2218 NGPJeXVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWPvSlV7UUN3ME2wMlc6OTNzIN88US=> NETTPIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
KNS-81-FD MkXZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2OxPGlEPTB;MD64NVAyOSEQvF2= NEfCTIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
HCC2157 MnyzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NETB[GZKSzVyPUCuPFQ6PTFizszN MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
BT-474 MonsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTBwOEi4OVQh|ryP NGewT5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
NCI-H748 MnrYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnKxTWM2OD1yLkmxNkDPxE1? NIDvXJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw2OTN7MEmvK|5USU6JRWK8M4E,
EW-24 MmHqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjmTWM2OD1yLkmyNFAzKM7:TR?= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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OMC-1 MmjpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVrJR|UxRTBwOU[zO|Mh|ryP M3:1O|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
NCI-H2196 MnWxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX3JR|UxRTBwOUe3O|ch|ryP NV3hR|FMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
HAL-01 MoLDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\FTWM2OD1yLkm5PFczKM7:TR?= Ml\WQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
NCI-H2107 NFH3[pdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnoUWRKSzVyPUGuNFE5PTRizszN Mn33QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
LB996-RCC NYG5XlB1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3sbZhKSzVyPUGuNFgxPTFizszN NX3rOo16RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
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CHP-126 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLCTWM2OD1zLkG2NVk6KM7:TR?= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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CAS-1 Mli1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFzvRXJKSzVyPUGuN|QzOzFizszN NWDOOWdKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
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DMS-153 M2\3W2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXHJR|UxRTFwNUe2O|Qh|ryP M3u4flxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFUyOzlyOT:nQnNCVkeHUkyvZV4>
SK-MEL-2 NHXhZ5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEjGW29KSzVyPUGuO|E4QDlizszN Mon2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
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NCI-H1092 M3rRbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M176eWlEPTB;MT63OlE5OiEQvF2= MlvDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
LU-165 M1jIfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M175VGlEPTB;MT64OlQ4PiEQvF2= NXL6[Gp2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
HL-60 NFL0cHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX75ZYQ1UUN3ME2xMlk6QTB6IN88US=> MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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THP-1 MmXpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnTBTWM2OD1{OT63NlAyKM7:TR?= MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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COLO-824 MlLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVXab3V{UUN3ME2zOE4yODB4IN88US=> NVLFXZFDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNPTF|OUC5M{c,W0GQR1XSQE9iRg>?
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KG-1 M4rHeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mm\uTWM2OD12MD64PVU2KM7:TR?= MkjkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMOVE{QTB7Lze+V2FPT0WUPD;hQi=>
TUR MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4CzZ2lEPTB;NEGuOlM6QSEQvF2= MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly1NVM6ODlxJ{7TRW5ITVJ:L3G+
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LNCaP  MnvNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NITkWmlKSzVyPUmxMlMhdk1? Mk\HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV3MEWxO|QoRjJ3NUC1NVc1RC:jPh?=
C4-2 NFnmO5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;lTWM2OD16IH7N NInNe3g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NUWwOVE4PCd-MkW1NFUyPzR:L3G+
JURKAT NXzINVl6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXSyOEBp MUnJR|UxRTRzNj62OkBvVQ>? NE\VWHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
MOLT-4 NXzWNWE2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXOyOEBp NHPVSGZKSzVyPUK4OVMvPSCwTR?= Mmm1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
REH MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVWyOEBp MorWTWM2OD1{OEeuOlghdk1? NV32[lhjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
NALM-6 MnTiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWKyOEBp M1nNPGlEPTB;M{SzN|Ahdk1? NGrwOoo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
RS 4;11 MmL3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MViyOEBp MULJR|UxRTVzOT6wOUBvVQ>? MoDHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
697 NEDoRZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHP[XJXOjRiaB?= MU\JR|UxRTJzMEeuNUBvVQ>? NFruXIQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
SIL-ALL Mn7OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7kNlQhcA>? NFKxcW1KSzVyPUG5PU44PyCwTR?= M1jkeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
CEM MkK0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmL5NlQhcA>? MUjJR|UxRTV{LkG4JI5O NIPGPGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
P12 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TUVlI1KGh? M3fGd2lEPTB;M{muNlchdk1? M2PhfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
NALM-16 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnfnNlQhcA>? Mnm2TWM2OD17Nj6yPEBvVQ>? NWLDVGRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
JURKAT NVi3fHV[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2m1V|Q5KGh? M1LHeWlEPTB;MUSuPFQhdk1? MnqyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
MOLT-4 NI[5cmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvtS21TPDhiaB?= NULtOnl4UUN3ME2yOk43KG6P NYjiNWM2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
REH MnHhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYi0PEBp MlPJTWM2OD12ND63NUBvVQ>? MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
NALM-6 M2TSb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXm0PEBp MUTJR|UxRTJ|LkK2JI5O MoH2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
RS 4;11 NHLX[FBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4C0NlQ5KGh? MoC2TWM2OD1zNkSuNlghdk1? M{X0bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
697 Mn\tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlfIOFghcA>? M4\RSWlEPTB;M{C1MlMhdk1? MlLZQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
SIL-ALL M3P0dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MX20PEBp MkftTWM2OD15ND62NkBvVQ>? MlzSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
CEM MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1\hXFQ5KGh? MVXJR|UxRTJ2LkW1JI5O NGXM[XM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWxPVk6PSd-MkS1NVk6QTV:L3G+
P12 NGi5NVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXy0PEBp M1LmW2lEPTB;MUiuOFYhdk1? Mk[wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
NALM-16 NFTIdmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH63bY01QCCq NH;odG5KSzVyPU[zMlI1KG6P MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
JURKAT NV7HS4dUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGrWbIk4OiCq M2jycGlEPTB;Mz62OUBvVQ>? MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
MOLT-4 NFr1cZBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4j3SFczKGh? MULJR|UxRTdwM{mgcm0> NVXid3RMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
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NALM-6 NH7rTIhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUO3NkBp M1fjO2lEPTB;MT63PEBvVQ>? Mnr1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
RS 4;11 M1rWWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoO3O|IhcA>? NIX2do1KSzVyPUSuN|ghdk1? M164bVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
697 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{K5NlczKGh? MV3JR|UxRTd2LkK4JI5O MonOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR3MUm5PVUoRjJ2NUG5PVk2RC:jPh?=
SIL-ALL M3nMUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{fkdlczKGh? NEPkZWhKSzVyPUeuNkBvVQ>? MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
CEM MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYfVZnZUPzJiaB?= NYfWPZRbUUN3ME21MlQzKG6P M1LQW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2NUG5PVk2Lz5{NEWxPVk6PTxxYU6=
P12 NYXDeXRHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmjSO|IhcA>? MV7JR|UxRTZwM{egcm0> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDVzOUm5OUc,OjR3MUm5PVU9N2F-
NALM-16 NELqPXlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGTqNGg4OiCq MmDWTWM2OD15Nz63N{BvVQ>? NV\vVphKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NVk6QTVpPkK0OVE6QTl3PD;hQi=>
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WER-Rb-1 MoH5RZBweHSxc3nzJGF{e2G7 MmTCNU02OCCwTR?= MWKxNk8zPCCq M3j1d2ROW09? NHPnUFRqdmS3Y3XkJIFxd3C2b4Ppd{B4cGmlaDDjZY4h[mViZX7oZY5k\WRiYomgTHQ> MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDN4Nk[2OUc,OjR|Nk[2OlU9N2F-
G7 NVXSNFA6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLPd2xWUUN3ME2zMlMhdk1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJyNEezN{c,OjR{MES3N|M9N2F-
G166 M2XQSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTdwMDDuUS=> NIn3d4w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKwOFc{Oyd-MkSyNFQ4OzN:L3G+
CB660 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYDmPFdOUUN3ME22MlQhdk1? NH30U2Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEKwOFc{Oyd-MkSyNFQ4OzN:L3G+
CB1117 NVX3bHJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYm0[HpNUUN3ME2xOU43KG6P NXOy[HRlRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyNFQ4OzNpPkK0NlA1PzN|PD;hQi=>
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SJ-GBM2 NYrsS3d{eUiWUzDhd5NigQ>? NEW5UotyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCFb37mbZJu[XSxcomgd4Nz\WWwIH\vdkBUUi2JQl2yJINmdGy| MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
TC32 NHL1WnVyUFSVIHHzd4F6 Mnr1dWhVWyCxZjDw[YRq[XS{aXOgZ4Fv[2W{IHPlcIwhdGmwZYOgeI8hcWSnboTp[pkhdXWudHnwcIUhd3Cyb4L0eY5qfGmnczDmc5Ih\HK3ZzDy[ZB2enCxc3nu[|ohS2:wZnnycYF1d3K7IIPjdoVmdiCob4KgWGM{OiClZXzsdy=> NGPTOVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
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Saos-2 MWXxTHRUKGG|c3H5 MVjxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBEd26oaYLtZZRwenlic3Py[YVvKG[xcjDTZY9{NTJiY3XscJM> NWrVRZBERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkm0N|UyOzlpPkK5OFM2OTN7PD;hQi=>
DU145 NF3vUoJCeG:ydH;zbZMh[XO|YYm= MWKxNFAhdk1? M3eyT|I1KGi{cx?= NWO4eWhoUW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDEWVE1PSClZXzsd{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDQRXJRKGOuZXH2ZYdmKGG2IEGwNEBvVSCjZoTldkAzPCCqcoOgZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:m MkPiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4OEW5OFEoRjJ3Nki1PVQyRC:jPh?=
HeLa MojzSpVv[3Srb36gZZN{[Xl? NUDPc5Q{OTByIH7N Ml\2TY5pcWKrdHnvckBw\iCSTFuxJHRpejJzMDDwbI9{eGixconsZZRqd25iaX6gbJVu[W5iSHXMZUBk\WyuczDheEAyODBibl2gZpkhcW2vdX7vZoxwfHSrbnegcYV1cG:m MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
DU145 NXXHXGVHTnWwY4Tpc44h[XO|YYm= M3z5ZVExOCCwTR?= Mn;1TY5pcWKrdHnvckBw\iCSTFuxJJBzd3SnaX6gZYNkfW23bHH0bY9vKGmwIHj1cYFvKESXMUS1JINmdGy|IHH0JFExOCCwTTDifUBqdW23bn;icI91fGmwZzDt[ZRpd2R? Mly4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjV4OEW5OFEoRjJ3Nki1PVQyRC:jPh?=
HeLa NGLkPYxHfW6ldHnvckBie3OjeR?= M2CxblExOCCwTR?= NYP6XlB{UW6qaXLpeIlwdiCxZjDQUGsyKHC{b4TlbY4h[WOldX31cIF1cW:wIHnuJIh2dWGwIFjlUIEh[2WubIOgZZQhOTByIH7NJIJ6KGmvbYXuc4Jtd3S2aX7nJI1mfGixZB?= NVfib3RqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkW2PFU6PDFpPkK1Olg2QTRzPD;hQi=>
HeLa M2fhZmZ2dmO2aX;uJIF{e2G7 MofuNVAxKG6P NFfNVHgyQCCqcoO= NY\MTYI6UW6mdXP0bY9vKG:oIH3vco9xd2yjcjDtbZRwfGmlIIPwbY5ldGW|IHnuJIh2dWGwIFjlUIEh[2WubIOgZZQhOTByIH7NJIlv[3WkYYTl[EBnd3JiMUigbJJ{KGK7IHnu[Ilz\WO2IHntcZVvd2[udX;y[ZNk\W6lZTDtbYNzd3Olb4D5 MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
HeLa NF;XXFlCeG:ydH;zbZMh[XO|YYm= NHnNUI8yODBibl2= NVv4bJozOjRiaILz NYfT[G53UW6mdXP0bY9vKG:oIHHwc5B1d3OrczDpckBpfW2jbjDI[WxiKGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIGDBVnAh[2ynYY\h[4Uh[XRiMUCwJI5OKGGodHXyJFI1KGi{czDifUBqdW23bn;icI91fGmwZzDt[ZRpd2R? NIDuR5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4OVk1OSd-MkW2PFU6PDF:L3G+
PC3 NIDCe|NHfW6ldHnvckBie3OjeR?= MUixNFAhdk1? NYezRo9mOThiaILz NXrwbYViUW6mdXP0bY9vKG:oIH3vco9xd2yjcjDtbZRwfGmlIIPwbY5ldGW|IHnuJIh2dWGwIGDDN{Bk\WyuczDheEAyODBibl2gbY5kfWKjdHXkJIZweiBzODDodpMh[nliaX7kbZJm[3RiaX3teY5w\my3b4Lld4NmdmOnIH3pZ5Jwe2OxcIm= NF6yd2Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NU[4OVk1OSd-MkW2PFU6PDF:L3G+
DU145 NFGx[nZHfW6ldHnvckBie3OjeR?= M3HT[VExOCCwTR?= NHrPPGZKdmirYnn0bY9vKG:oIGDMT|EhXGi{MkGwJJBpd3OyaH;yfYxifGmxbjDpckBpfW2jbjDEWVE1PSClZXzsd{BifCBzMECgcm0h[nliaX3teY5w[myxdITpcochdWW2aH;k MVy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPTZ6NUm0NUc,OjV4OEW5OFE9N2F-
Assay
Methods Test Index PMID
Western blot pro-caspase-8 / cleaved caspase-8 / cleaved caspase-3 / cleaved caspase-9 ; p-HH3 / PARP / Myc / p-Myc ; pMEK / MEK / pERK / ERK / pRSK1 / RSK1 ; pPlk1 / Plk1 / pMyt1 ; Aurora B / pAurora B / pAurora C / pH3 / Cyclin B1 / Securin / pBcl-XL / MPM2 27699933 24771642 28430578 25169932
Immunofluorescence p-H2AX / propidium iodide ; Pericentrin / α-tubulin 22309939 21772266
Growth inhibition assay Cell viability 21772266
In vivo

BI 2536 given i.v. once or twice per week is highly efficacious in diverse xenograft models with acceptable tolerability by inhibiting cell proliferation through a mitotic arrest, and subsequently induction of tumor-cell death. Administration of BI 2536 at 50 mg/kg once or twice per week significantly inhibits growth of HCT 116 xenografts with T/C of 15% and 0.3%, respectively. BI 2536 treatment twice-weekly also leads to excellent tumor-growth in BxPC-3 and A549 models with T/C of 5% and 14%, respectively. [1]

Protocol (from reference)

Kinase Assay:

[1]

  • Plk1 in vitro kinase assay:

    Recombinant human Plk1 (residues 1-603) is expressed as N-terminal, GST-tagged fusion protein with a baculoviral expression system and purified by affinity chromatography with Glutathione-agarose. Enzyme activity assays for Plk1 are performed in the presence of serially diluted BI 2536 with 20 ng of recombinant kinase and 10 μg casein from bovine milk as the substrate. Kinase reactions are performed in a final volume of 60 μL for 45 minutes at 30 °C (15 mM MgCl2, 25 mM MOPS [pH 7.0], 1 mM DTT, 1% DMSO, 7.5 mM ATP, 0.3 μCi γ-33P-ATP). Reactions are terminated by the addition of 125 μL of ice-cold 5% TCA. After transfer of the precipitates to Multi-Screen mixed ester cellulose filter plates, plates are washed with 1% TCA and quantified radiometrically. Dose-response curve is used for calculating IC50 value.

Cell Research:

[1]

  • Cell lines: HeLa, A43, SKOV-3, HT-29, K562, A549, Saos-2, MCF7, HCT116, COLO 205, Hep G2, Raji and PC-3 cells, etc.
  • Concentrations: Dissolved in DMSO, final concentrations ~1 μM
  • Incubation Time: 24 and 72 hours
  • Method:

    Cells are exposed to various concentrations of BI 2536 for 24, and 72 hours. Cell growth is assessed by the measurement of Alamar Blue dye conversion in a fluorescence spectrophotometer. For determining the DNA content of the cultures, cell suspensions are fixed in 80% ethanol, treated for 5 minutes with 0.25% Triton X-100 in PBS, and incubated with 0.1% RNase and 10 μg/mL propidium iodide (PI) in PBS for 20 minutes at RT. Cell-cycle profiles are determined by flow cytometric analysis.

Animal Research:

[1]

  • Animal Models: Female BomTac:NMRI-Foxn1nu mice injected subcutaneously with HCT 116, NCI-H460, or A549 cells
  • Dosages: ~50 mg/kg
  • Administration: Injection i.v. once or twice per week

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
5%DMSO+40%PEG300+5%Tween80+50%ddH2O
For best results, use promptly after mixing.

12.5mg/mL

Chemical Information

Molecular Weight 521.66
Formula

C28H39N7O3

CAS No. 755038-02-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CCC1C(=O)N(C2=CN=C(N=C2N1C3CCCC3)NC4=C(C=C(C=C4)C(=O)NC5CCN(CC5)C)OC)C

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02215044 Terminated Drug: BI 2536 intravenous|Drug: Gemcitabine intravenous Pancreatic Neoplasms Boehringer Ingelheim June 2007 Phase 1
NCT02211833 Completed Drug: BI 2536|Drug: Pemetrexed Carcinoma Non-Small-Cell Lung Boehringer Ingelheim October 2006 Phase 1
NCT00701766 Completed Drug: BI 2536 Leukemia Myeloid Acute Boehringer Ingelheim October 2006 Phase 2
NCT00706498 Completed Drug: BI 2536 Prostatic Neoplasms Boehringer Ingelheim September 2006 Phase 2
NCT00376623 Completed Drug: BI 2536 Carcinoma Non-Small-Cell Lung Boehringer Ingelheim July 25 2006 Phase 2
NCT02211872 Completed Drug: BI 2536 BS intravenous Neoplasms Boehringer Ingelheim August 2004 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
We need to use BI 2536 in preclinical studies using mice, how to formulate the inhibitor for this in vivo study?

Answer:
For in vivo study, DMSO should not be more than 5% because the toxicity. You can formulated BI2536 in hydrochloric acid (0.1 N), and diluted with 0.9% NaCl for animal study according to the reference :( Supplemental Data) http://linkinghub.elsevier.com/retrieve/pii/S0960-9822(06)02671-6

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