Catalog No.S9915 Synonyms: FSY-NH2

For research use only.

FSLLRY-NH2 TFA (FSY-NH2) is a selective protease-activated receptor 2 (PAR2) inhibitor with an ic50 of 50 µM in PAR2-KNRK cells.

FSLLRY-NH2 TFA Chemical Structure

CAS No. 245329-02-6 (free base)

Purity & Quality Control

Choose Selective PAR Inhibitors

Biological Activity

Description FSLLRY-NH2 TFA (FSY-NH2) is a selective protease-activated receptor 2 (PAR2) inhibitor with an ic50 of 50 µM in PAR2-KNRK cells.
PAR2 [1]
(in PAR2-KNRK cells)
50 µM
In vitro

FSLLRY-NH2 blocks the ability of trypsin to activate PAR(2) either in PAR(2)-expressing Kirsten virus-transformed kidney (KNRK) cell lines or in a rat aorta ring preparation. The mechanism of this activity does not involve a simple inhibition of trypsin proteolytic activity, but possibly by interacting with a tethered ligand receptor-docking site.<sup><a class="sref"href="#s_ref">[1]</a></sup>

In vivo

Treatment with FSLLRY-NH2 significantly improves neurological outcome and reduces the number of degenerating hippocampal neurons after ACA (asphyxial CA).<sup><a class="sref"href="#s_ref">[2]</a></sup>

Protocol (from reference)

Cell Research:

<sup><a class="sref" href="#s_ref">[1]</a></sup>

  • Cell lines: Kirsten virus-transformed kidney (KNRK) cells
  • Concentrations: 200 μM
  • Incubation Time: 1 h
  • Method:

    KNRK cells are incubated with 10 nM trypsin  at 37°C for 10 to 60 min in the presence or absence of 200 μM FSLLRY-NH2. The absorbance of the released resorufin, reflecting proteolytic activity, is measured using a Beckman DU 640B Spectrophotometer at 574 nm for assay of proteolytic activity of trypsin.

Animal Research:

<sup><a class="sref" href="#s_ref">[2]</a></sup>

  • Animal Models: ACA rat mode rats(ladult male Sprague-Dawley)
  • Dosages: 50 μg
  • Administration: i.n.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 910.98


CAS No. 245329-02-6 (free base)
Storage 3 years -20°C powder
2 years -80°C in solvent

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