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Batimastat (BB-94)

Name: Batimastat (BB-94)
Brand: Selleck Chemicals
SKU: S7155
Rating: 5 (26 reviews)

Catalog No.S7155

For research use only.

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. Also inhibits the activitity of other metalloproteases, such as ADAM17.

CAS No. 130370-60-4

Selleck's Batimastat (BB-94) has been cited by 26 publications

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MMP Signaling Pathway Map

Biological Activity

Description

Targets

MMP-1 ^[1]	MMP-2 ^[1]	MMP-9 ^[1]	MMP-7 ^[1]	MMP-3 ^[1]
3 nM	4 nM	4 nM	6 nM	20 nM

In vitro

Batimastat (BB-94) is a potent, broad spectrum matrix metalloprotease (MMP) inhibitor for MMP-1, MMP-2, MMP-9, MMP-7 and MMP-3 with IC50 of 3 nM, 4 nM, 4 nM, 6 nM and 20 nM, respectively. ^[1]Batimastat exhibits an unexpected binding geometry, with the thiophene ring deeply inserted into the primary specificity site. ^[2]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID

Sf9 insect cells	M4\GO2Z2dmO2aX;uJIF{e2G7			NWDmWG1EUW6qaXLpeIlwdiCxZjDoeY1idiCvYYTybZghdWW2YXzsc5Bzd3SnYYPlMVIh\XiycnXzd4VlKGmwIGPmPUBqdnOnY4SgZ4VtdHNuIFnDOVA:OC5yMUOg{txO	M1fnRVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NUiyOFM3Lz5zNUW4NlQ{PjxxYU6=
Sf9 insect cells	Mk\jSpVv[3Srb36gZZN{[Xl?			NGrIO2JKdmirYnn0bY9vKG:oIHj1cYFvKG2jdILpfEBu\XSjbHzvdJJwfGWjc3WtPUBmgHC{ZYPz[YQhcW5iU3[5JIlve2WldDDj[YxteyxiSVO1NF0xNjB{NTFOwG0>	M13sblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NUiyOFM3Lz5zNUW4NlQ{PjxxYU6=
HT1080	NIr6WHJHfW6ldHnvckBie3OjeR?=			M3jFW2lvKH[rdILvJIlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBu[XS{aYigcYV1[Wyub4Dyc5Rm[XOnIEKgbZNwdGG2ZXSg[pJwdSCqdX3hckBJXDFyOECg[oljem:\|YYLjc41iKGOnbHzzJIlv\HWlZXSge4l1cCCWTl[sJGlEPTB;MD6wNFIy\|ryP	NUDLUoVYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxOUi3N\|cyOid-OUi3N\|cyOjxxYU6=
white blood cells	Ml3TRY51cW[rYoLveIlkKGG\|c3H5	M3;1TFI2KHSxIEG1NEBu\y:tZx?=	MmHtNVMh\GG7cx?=	Ml;HRY51cW[rYoLveIlkKGGldHn2bZR6KGmwIHLs[Y9ugWOrbj3pcoR2[2WmIFO1O2IyNzZibX;1d4UhdW:mZXygZZN{\XO\|ZXSgZZMhcW6qaXLpeIlwdiCxZjD3bIl1\SCkbH;v[EBk\WyuczDpckBjem:wY3jvZYx3\W:uYYKgcIF3[WenIHH0JFI2KHSxIEG1NEBu\y:tZzygdI8h[mmmIHHkcYlvcXO2ZYLl[EAzKGi{czDwdolweiCkbHXvcZlkcW5iaX7keYN1cW:wIH\vdkAyOyCmYYnz	NX\se3MxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW1PFI1OzZpPkG1OVgzPDN4PD;hQi=>
RPM18866	M4\nWmZ2dmO2aX;uJIF{e2G7			M{f4WXRme3SnZDDmc5IhcW6qaXLpeIlwdiCjZ3HpcpN1KEOGMkOgLGloTSC{ZXPldJRweilicILveIVwdHm\|aYOgbY4hdWWvYoLhcoV{KGSncnn2[YQh\nKxbTDSVG0yQDh4NjDj[YxteyBqIHGgbJVu[W5iQj3j[YxtKGyrbnWpMEBKSzVyPUCuNe69VQ>?	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek86QDdzNkKyK\|46QDdzNkKyQE9iRg>?
RPM18866	MUPGeY5kfGmxbjDhd5NigQ>?			MmrYTY5pcWKrdHnvckBi\2GrboP0JGNFOjNiKFnnSUBz\WOncITvdkkheHKxdHXvcJl{cXNiaX6gcYVu[nKjbnXzJIRmemm4ZXSg[pJwdSCUUF2xPFg3PiClZXzsd{whUUN3ME2wMlHPxE1?	NHPObHU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi97OEexOlI{Lz57OEexOlI{RC:jPh?=

Click to View More Cell Line Experimental Data

In vivo

Batimastat can inhibit metastatic spread and growth of B16-BL6 murine melanoma. ^[1] In an orthotopic colon tumor model in mice, timastat treatment results in inhibition of primary tumor growth (by 50%), local/regional spread(from 67% to 35%), and distant metastasis(from 30% to 10%).^[3]Batimastat reduces in vivo growth of experimental hemangiomas, most probably by blocking endothelial cell recruitment by the transformed cells or by interfering with cell organization in vascular structures. ^[4]

Protocol (from reference)

Animal Research:^[3]	Animal Models: Nude mouse Dosages: 30mg/ml Administration: i.p.

References

[4] Taraboletti G, et al. J Natl Cancer Inst, 1995, 87(4), 293-298.

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Solubility (25°C)

In vitro


In vivo	Add solvents to the product individually and in order (Data is from Selleck tests instead of citations): 2% DMSO+40% PEG 300+2% Tween 80+ddH2O For best results, use promptly after mixing. Click to purchase: PEG300 Tween 80	2mg/mL

Chemical Information

Molecular Weight	477.64
Formula	C₂₃H₃₁N₃O₄S₂
CAS No.	130370-60-4
Storage	3 years	-20°C	powder
Storage	2 years	-80°C	in solvent
Smiles	CC(C)CC(C(CSC1=CC=CS1)C(=O)NO)C(=O)NC(CC2=CC=CC=C2)C(=O)NC

Download Batimastat (BB-94) SDF

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

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Dilution Calculator Molecular Weight Calculator

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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