SB-3CT
For research use only.
Catalog No.S7430
18 publications
CAS No. 292605-14-2
SB-3CT is an effective and selective gelatinase inhibitor with Ki of 13.9 nM and 600 nM for MMP-2 and MMP-9, respectively.
4 Customer Reviews
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The matrix metalloproteinase 9(MMP9) inhibitor(SB-3CT) attenuated angiotensin II (Ang II)-induced cardiomyocyte hypertrophy in vitro. (A) Representative immunofluorescent (α-actinin) images of neonatal rat cardiomyocytes (NRCMs) in the presence or absence of MMP9 inhibitor that were administered PBS or Ang II for 48 h. Bar=200 μm. (B) Statistical results for the cell surface area (CSA) of NRCMs. (C-E) The relative mRNA expression levels of atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and β-myosin heavy chain (β-MHC) in NRCMs in the presence or absence of MMP9 inhibitor that were administered PBS or Ang II for 48 h. The intensities were normalized to the levels of GAPDH mRNA with the value in the PBS group defined as 1. * P<0.05 versus the control group, # P<0.05 versus the Ang II group. n=6 samples per experimental group
Cell Physiol Biochem, 2017, 44(6):2281-2295. SB-3CT purchased from Selleck.
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A) Inhibitory effect of SB‐3CT at the indicated concentrations against migration of HUVECs analyzed by a wound‐healing assay. These photos were obtained under fluorescence microscopy (×100) at 0 and 24 h after scraping. Bar = 200 μm.
Mol Nutr Food Res, 2018, doi:10.1002/mnfr.201700969. SB-3CT purchased from Selleck.
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B) Representative immunoblot and quantitative analysis of the effect of SB-3CT on the decrease in claudin-5 (25 kDa) and (C) AQP4 (35 kDa) expressions induced by MDMA at 1 h (n=4-6).
Neuropharmacology, 2017, 118:157-166. SB-3CT purchased from Selleck.
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EdU assays of directly co-cultured EOC cells with or without SB-3CT were performed. EOC cells cultured alone were used as controls. SB-3CT mediated MMP2/9 inhibition partially offset the proliferation-promoting effects of ADSCs in EOC.
Exp Cell Res, 2018, 337(1):16-27. SB-3CT purchased from Selleck.
Purity & Quality Control
Choose Selective MMP Inhibitors
Biological Activity
| Description | SB-3CT is an effective and selective gelatinase inhibitor with Ki of 13.9 nM and 600 nM for MMP-2 and MMP-9, respectively. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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| In vitro |
SB-3CT directly inhibits bone marrow endothelial cell invasion and tubule formation in Matrigel in vitro. [3] |
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| Cell Data |
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| Assay |
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| In vivo | In L-CI.5s T-cell lymphoma model, SB-3CT (5-50 mg/kg/d i.p.) potently inhibit liver metastasis and increases survival of mice. [2] SB-3CT (50 mg/kg/d i.p.) inhibits human prostate cancer growth, osteolysis and angiogenesis in a bone metastasis model. [3] In a mouse model of embolus-induced “permanent” focal cerebral ischemia, SB-3CT counteracts degradation of neuronal laminin, protects neurons from ischemic cell death, and ameliorates neurobehavioral outcomes after embolic MCA occlusion. [4] |
Protocol
| Animal Research:[2] |
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Solubility (25°C)
| In vitro | DMSO | 61 mg/mL (199.08 mM) |
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| Ethanol | 10 mg/mL warmed (32.63 mM) | |
| Water | Insoluble | |
| In vivo | Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 4% DMSO+corn oil For best results, use promptly after mixing. |
10mg/mL |
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
Chemical Information
| Molecular Weight | 306.40 |
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| Formula | C15H14O3S2 |
| CAS No. | 292605-14-2 |
| Storage |
powder in solvent |
| Synonyms | N/A |
| Smiles | C1C(S1)CS(=O)(=O)C2=CC=C(C=C2)OC3=CC=CC=C3 |
In vivo Formulation Calculator (Clear solution)
| Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment) | ||||||||||
| Dosage | mg/kg |  |
Average weight of animals | g | |
Dosing volume per animal | ul | |
Number of animals | |
| Step 2: Enter the in vivo formulation () | ||||||||||
| % DMSO % % Tween 80 % ddH2O | ||||||||||
| CalculateReset | ||||||||||
Calculation results:
Working concentration： mg/ml；
Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL，)
Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH2O，mix and clarify.
1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.
Bio Calculators
Molarity Calculator
Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:
Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)
*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).
Dilution Calculator
Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )
* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).
Molecular Weight Calculator
Enter the chemical formula of a compound to calculate its molar mass and elemental composition:
Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2
Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.
Molarity Calculator
Tech Support
Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.
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